receptor mediated delivery systems for cancer therapeutics

... Laser II Viral and non-viral delivery systems (Non-physical delivery methods) including: Biological gene delivery systems (viral vectors) Non-biological gene delivery systems (non-viral vectors) ... http://www.molecular -cancer. com/content/10/1/3 Page of 20 Figure Live/non-live delivery systems Live or biological gene delivery systems include viral and/or bacterial vectors Non-live or nonbiological delivery systems ... polymers have been designated as feasible candidates for drug delivery systems, anti -cancer agents and vaccine immunotherapy For example, DNA vaccine delivery to APCs has been facilitated by microencapsulation...

Ngày tải lên: 06/03/2014, 02:21

... suitable candidates for a nanoparticle-based drug delivery system to improve their pharmacological performance For this purpose, a “longboat delivery system” has been prepared for the platinum warhead ... reaching the cancer focuses Furthermore, the solubilizer cremophor in current formulation (Taxol) has exhibited allergenic activity, prompting the search for alternative delivery systems For this ... reached about whether CNTs are the best carriers in anticancer drug delivery systems because there are many other anticancer drug delivery systems based on other nanomaterials, such as cationic...

Ngày tải lên: 20/06/2014, 22:20

... nutraceuticals for cancer prevention, similar research is routinely accomplished in the search for new cancer drugs and drugs for other diseases Cell kinetics is the repetitive cycle (time) for cell ... Table 3.4 This information is similar to the information presented for a food ingredient/nutraceutical petition for FDA self-afÞrmed Generally Recognized as Safe (GRAS) status or for a health claim ... common tag line for these products is “Stimulation for Body and Mind.” The term stimulator beverage was chosen for this review rather than the more common terms of energy drink or performance enhancer...

Ngày tải lên: 25/10/2013, 21:20

... Gene delivery systems 14 Viral gene delivery systems .14 Non-viral gene delivery systems 14 Naked (unformulated) nucleic acids .15 Barriers to non-viral gene delivery ... delivery systems for gene therapy of genetic or acquired human diseases has motivated the search for, and utilisation of, safer carrier molecules Non-viral delivery systems such as naked or formulated ... various problems for successful clinical gene therapy [15,16] 13 Gene delivery systems Viral gene delivery systems Viral gene delivery systems are based on recombinant viruses that are genetically modified...

Ngày tải lên: 23/08/2015, 17:29

... light delivery systems In this thesis a new light delivery system consisting of the nano-aperture vertical-cavity surface emitting laser (VCSEL) as a potential candidate for an alternative light delivery ... Wakil Shehzad for their guidance, help and encouragement A special thanks to my i best friend Ms Nurjiha, for always being with me during all the ups and downs I owe these people a lot for everything ... power) in the far-field [53] Therefore it cannot be used for high power applications; a 50-nm circular aperture has a transmission efficiency less than 10-5 for visible light [54] Betzig et al...

Ngày tải lên: 09/09/2015, 08:17

... BACTERIAL VECTORS FOR ANTIGEN DELIVERY 22 1.5.1 Rationale for using a live bacterial vector 22 1.5.2 Attenuated pathogens 24 1.5.2.1 B pertussis as a live vector for nasal delivery of heterologous ... BORDETELLA PERTUSSIS AS A LIVE VECTOR FOR THE PRODUCTION AND MUCOSAL DELIVERY OF DENV2 EDIII 4.3 147 LACTOCOCCUS LACTIS AS A LIVE VECTOR FOR THE PRODUCTION AND MUCOSAL DELIVERY OF DENV2 EDIII 153 CHAPTER ... therefore indicated that BPZE1 was not ideal for the presenting EDIII to the respiratory mucosal immune system As an alternative approach, we chose the lactic acid bacterium L lactis for EDIII delivery...

Ngày tải lên: 09/09/2015, 08:18

... (Butler, 2011) For men, lung and bronchus cancer has the highest occurrence and fatality rate worldwide among the cancer incidents; for women, breast cancer holds the top position (American Cancer Society, ... highest in terms for fatality for men, there is a high rate of reoccurrence and lack of targeting ligands for bladder cancer that can be applicable for various types of bladder cancers Hence, it ... aptamer L3 has good potential for targeted drug therapeutics Currently, there is limited information in the literature on targeting ligands for specific bladder cancer drug delivery Hence, in Chapter...

Ngày tải lên: 09/09/2015, 10:21

... drug and gene delivery Expert opinion on drug delivery 2010; 7(2), 159-171 Wang Z., Chui WK Ho PC Nanoparticulate delivery system targeted to tumor neovasculature for combined anticancer and antiangiogenesis ... nanoparticle for targeted chemotherapeutic delivery of paclitaxel (Submitted for review) Wang Z., Liew G F., Ho PC Double targeting cRGD-HA-DSPE self-assembled PLGA core-shell nanoparticle for the delivery ... invasive cancers (3) 1.1.2 Metastasis of cancer Metastasis, the spread of cancer from its primary site to a distant organ, is responsible for the major cause of morbidity and mortality of cancer...

Ngày tải lên: 11/09/2015, 09:58

... scaffolds as drug delivery systems for rhBMP-2 Biomaterials 2005; 26(17): 3739-3748 B Rai, S.H Teoh and K.H Ho An in vitro evaluation of PCL-TCP composites as delivery systems for platelet-rich ... PCL-TCP scaffolds 164 9.1.3 PCL-TCP scaffolds as delivery systems for a single growth factor 166 9.1.4 PCL-TCP scaffolds as delivery systems for multiple growth factors 167 9.1.5 Rat femoral ... protein delivery systems for the repair 28 of bone tissue The merit of the work was that the PCL-based scaffolds could serve not only as a template for bone growth but also as a delivery vehicle for...

Ngày tải lên: 15/09/2015, 17:10

... them to serve as "Trojan horses" for the delivery of genes for cancer therapy Indeed, clostridial spores that were genetically manipulated to harbour genes for cancerstatic factors, prodrug converting ... and the foreseeable promises and problems when compared with existing viral vector systems The development and use of viral vector systems for cancer gene therapy (A) Retroviral vector systems ... the Ludwig Center for Cancer Genetics & Therapeutics, the Johns Hopkins Kimmel Cancer Center, Baltimore, Maryland, USA for instrumental comments on the use of clostridium for oncolytic therapy...

Ngày tải lên: 14/08/2014, 19:22

... denaturation at 94 °C for min, followed by 32 cycles of 98 °C for 10 s, 55 °C for 20 s and 60 °C for 20 s The amplified PCR products (expected size 168 bp) were analyzed by performing agarose gel ... Japan) at 42 °C for 60 after the initial denaturation at 72 °C for min, followed by heat inactivation of enzyme at 99 °C for The cDNAs were diluted and further utilized as the templates for quantitative ... cells within 10 through the FP receptor Enhanced de novo synthesis of PGF2a and PGE2 by activation of FP receptor MEK ⁄ ERK cascade Next we examined whether FP receptor- mediated activation of the...

Ngày tải lên: 28/03/2014, 22:20

... formation much By contrast, these peptides inhibited SNARE complex formation very efficiently when added before preincubation Therefore, N-terminal-mimicking peptides inhibited SNARE complex formation ... parental SNARE proteins for SNARE complex formation, inhibiting SNARE assembly In an initial attempt to search for the potent peptide sequences that inhibit SNARE complex formation and neurotransmitter ... analysis of SNARE complex formation was performed after the membrane fusion assay in order to confirm that the N-terminal-mimicking peptides inhibited SNARE complex formation Numbers below the...

Ngày tải lên: 30/03/2014, 04:20

... metastatic breast cancer The fluorescence uptake by the MCF-7 cells could provide a useful model to assess the in vitro therapeutic effect of the Taxoids in the various formulations for breast cancer treatment ... (ONT-093) Cancer Chemother Pharmacol 2005, 55(1):72-78 Feng SS, Mei L, Anitha P, Gan CW, Zhou W: Poly (lactide)-vitamin E derivative/montmorillonite nanoparticle formulations for the oral delivery ... DMAB-modified PLGA-TPGS nanoparticle suspension at 37°C for h, the cells were rinsed with cold PBS for three times and then fixed by ethanol for 20 The cells were further washed twice with PBS,...

Ngày tải lên: 21/06/2014, 08:20

... computed for each of the raters using the respective other raters as reference, that is, the mean of the four other raters for the LE group and the three other raters for the OC group For the ... frequency, is in a normal range, the formants and their range may be affected Sumita et al showed that the first formant (F1) is higher in the vowel /i/, and the second formant (F2) is lower in vowels ... included the excision of the tumour and additional radiotherapy for most of the patient except for T1 grading Recording was performed at least months after the treatment had been completed The...

Ngày tải lên: 21/06/2014, 20:20

... chi-square test for trend Results Cloning of novel alternative splice variants coding for secreted receptor isoforms To identify novel splice variants from cell surface receptor genes, we performed RT-PCR ... is in widespread use as a tool for developing new therapeutics Work in the acute CIA model formed the basis for the widespread clinical use of TNFα inhibitors for treatment of RA [29-32] Moreover, ... FGFR4ASV was 30 cycles of 95°C for 45 seconds, 60°C for 50 seconds, and 72°C for minute The reaction was terminated with an elongation step of 72°C for 10 minutes For quantitative RT-PCR, gene-specific...

Ngày tải lên: 09/08/2014, 10:23

... and information systems Other answers could come from comparing performance improvement for the eight topic areas created by QUERI with others not so targeted but still part of the VHA performance ... effort in progress, its delineation of strategies for different target audiences may be useful for others Berwick has described this scale-up/spread problem, identifying seven recommendations for ... 2009, 4:9 their use of a variety of performance measures to compare 12 community samples with 12 VA systems [2] Patients from the VHA scored significantly higher for adjusted overall quality (67%...

Ngày tải lên: 11/08/2014, 05:21

... coworkers [53,54] realized that if data for shRNA -mediated changes in cellular fitness were available for enough cancer cell lines representing two different classes (for example, K-Ras wild-type and ... of human cancer Conclusions In summary, synthetic lethality provides a conceptual framework for discovering drugs that selectively kill cancer cells while sparing normal tissues and for tackling ... framework for the development of drugs that will kill cancer cells (bearing the sensitizing mutation) while sparing normal cells (which not; Figure 1) Moreover, it provides a framework for pharmacologically...

Ngày tải lên: 11/08/2014, 12:20

... requirement for microtubules depends upon which phagocytic receptor is involved For example, inhibiting microtubule function blocks complement receptor- mediated, but not Fc receptor- mediated, particle ... them as type I (Fc receptor- mediated) and type II (complement receptor- mediated) Microtubule poisons such as nocodazole paralyze complement -mediated, but not Fc receptor- mediated, particle internalization ... necessary for SR -mediated phagocytosis by MØ The requirement for PI-3K and tyrosine kinases is consistent with a recent report showing that PI-3K and the Src kinase Lyn are both required for SR-Amediated...

Ngày tải lên: 12/08/2014, 15:21

... biological applications Advanced Drug Delivery Reviews 2001, 47:277-294 Cristano RJ, Curiel DT: Strategies to gene delivery via the receptor- mediated endocytosis pathway Cancer Gene Ther 1996, 3:49-57 ... production of growth factors Systemic gene delivery systems are needed for therapeutic applications in which the target cells are not directly accessible [3] However, for several reasons including lack ... (32.5 mg/kg) were performed hours and just a few minutes before intravenous injection of conjugates The tolerance of chloroquine (used to prevent the degradation of the plasmid for transfection assays,...

Ngày tải lên: 14/08/2014, 19:22

... 500 μl of chloroform were added to the suspension The mixture was maintained under agitation for 60 The sample was centrifuged at 14,000 rpm for and the supernatant was separated for analysis The ... Biodegradable polymers as drug delivery systems Edited by: Chasin M, Langer R New York, N.Y: Marcel Dekker; 1990:1-43 Barman SP, Lunsford L, Chambers P, Hedley ML: Two methods for quantifying DNA extracted ... Chemicals, Wankee, WI, USA) as surfactant, to form a stable water-in-oil in-water emulsion The mixture was stirred for h with a RW20 IKA homogenizer for solvent evaporation Microspheres were collected...

Ngày tải lên: 14/08/2014, 19:22