real time in situ studies of dissipative structures in

... in the movement of the C-terminal domain of CRP by % A towards the N-terminal domain, which in consequence leads to rearrangement of DNA-binding domains and cAMPbinding domains of the protein ... nucleotide-binding domain to the DNA-binding domain of the protein We have used single tryptophancontaining mutants of CRP The mutations were localized in the N-terminal domain at position 85 or 13 in ... cAMP-binding pocket of the N-terminal domain, and Cys178, uorescently labeled by AEDANS, localized in the helixturnhelix motif of the C-terminal domain of the protein The lifetimes obtained for...

Ngày tải lên: 20/02/2014, 23:20

... reproduction within Brachiaria makes it an interesting system for understanding the molecular pathways involved in both modes of reproduction The identification of genes involved in apomictic development ... present in more than 300 angiosperm species [10] and is being investigated by many groups due to the biotechnological interest of controlling the process of cloning through seeds The occurrence of ... high-throughput gene expression profiles [15,16] One of the methodologies to determine gene expression levels in qRT-PCR is by comparing the expression of the gene of interest in different conditions with...

Ngày tải lên: 12/08/2014, 03:20

... emergency handling Recorded emergency events were also used to provide the audio-video documentation of interventions In those situations, the use of real- time multimedia streaming in uences the ... usability From the GPRS network point of view, two different kinds of terminals were used for the test The smartphone having + timeslots with a CS2 coding scheme resulting in 53.6 kbps downstream and ... containing large motions and not in low complex sequences The obtained gain in complexity reduction is particularly favorable in slow-motion situations because of the restricted number of motion...

Ngày tải lên: 22/06/2014, 19:20

... complaints of spinal and leg bone pain, was recognized as a type of chronic toxicity induced by excess Cd contamination of drinking water and cereals (mainly rice) Since then, the contamination of ... with lines depicting the kinetics of initial arrival of Cd in the respective ROI The X-intercepts of the fitting lines were adopted as the arrival times of the theoretical “leading edge” of the ... quantification of the differences in whole-body Cd transport from the roots to the grains of intact plants within rice cultivars that differ in grain Cd concentrations, by using PETIS, a real- time imaging...

Ngày tải lên: 11/08/2014, 11:21

... 98 4.24 Cosine signatures for in- situ real- time control through the PEB process with spin coating at 5500 rpm at the wafer edge 98 xii 4.25 Averaged cosine signatures for in- situ real- time control ... baking at 130 ◦ C for 125 sec 91 4.15 Cosine signatures for in- situ real- time control through the PEB process at the wafer center 92 4.16 Cosine signatures for in- situ real- time ... conventional baking at 130 ◦ C for 125 sec with spin coating at 5500 rpm 97 4.23 Cosine signatures for in- situ real- time control through the PEB process with spin coating at 5500...

Ngày tải lên: 08/09/2015, 19:26

... trace of m/z 273 in Fig The more interesting thing is that the shape of individual pulses (indicated by the slope of the arrows) kept changing For example, at the beginning of the acetylation ... real- time, on-line monitoring of chemical reactions in industry, providing instant data for the feedback loop to correct possible reaction deviations In addition to real- time monitoring, tandem ... 301) and intermediate B (m/z 273) as a function of time are shown in Fig The spikes in these traces result from the intermittent sampling of the reaction mixture every 4–5 During one sampling cycle,...

Ngày tải lên: 14/02/2014, 08:20

... DNA bending [20] induced by dimer binding (61), a free energy of ()21.2 to 7.8) = )14.5 kJặmol)1 is expected for binding of a single DNA-binding domain without the need to force DNA bending This ... protein dimers not act as initiators in plasmid replication [21] A positive effect on monomer-binding afnity thus provides a way of selecting against dimer binding, favoring monomer binding and ... DNA-binding domains involved in binding), or 1DR-short (as a dimer, but presumably with only one domain involved), or indeed when bound as a monomer to 1DR This suggests that binding to both inverted...

Ngày tải lên: 07/03/2014, 04:20

... Related Work In the last decade, interest in mining sentiment and opinions in text has grown rapidly, due in part to the large increase of the availability of documents and messages expressing personal ... approach combines real- time data processing and statistical sentiment modeling informed by, and contributing to, an understanding of the cultural and political practices at work through the use of Twitter ... his ex-wife at the beginning of the debate Within minutes, our dashboard showed his negative sentiment increase rapidly – it became three times more negative in just two minutes This illustrates...

Ngày tải lên: 16/03/2014, 20:20

... detecting binary interactions, this approach was used to build multicomponent systems, as well as to identify the interacting domains of proteins Identifying the functions of protein domains may ... detection priniciple is shown in Fig This sensing technology has been used to detect the binding of single virus particles [102], small molecule binding to proteins (1 nm Gleevec in the presence of 100 ... the spotting of expression plasmids, instead of purified proteins, on the array surface and using a mammalian cell-free expression system to express the proteins in situ at the time of the assay...

Ngày tải lên: 23/03/2014, 15:21

... the interface superheating and undercooling, during which the RDF was tried to be applied in determining the interface Tint DT Vint Dz melt-solid interface temperature interfacial superheating/undercooling ... allowing the determination of the value of C1 Relatively little work being done to determine the interface velocity-superheating/undercooling function even for single-component materials because of ... difficulties in measuring the interface temperature during rapid resolidification, however, in order to analyze interface kinetics, the interface location and the interface temperature need to be determined...

Ngày tải lên: 06/05/2014, 08:54

... fit of separate address spaces in real- time operating systems,” in Proceedings of the 23rd IEEE Real- Time Systems Symposium (RTSS ’02), pp 124–133, Austin, Texas, USA, December, 2002 [28] U Steinberg, ... correct functioning of the system Delays in interrupt handling can lead to jitter in time- based operations, missed deadlines, and the lateness or loss of time- sensitive data Unfortunately, in many general-purpose ... according to strictly timed protocols should disable interrupts to avoid interferences Interrupts and system architecture In Linux, interrupts and device drivers can easily interfere with real- time...

Ngày tải lên: 22/06/2014, 19:20

... the following reasons: (1) It minimizes the establishment of infection by non-inhalation routes The probability of acquiring infection through non-inhalation routes, such as ingestion of test agent ... decontaminate an animal with a bleach (or similar) solution during inhalation exposure studies using other types of dissemination devices, (3) It reduces potential contamination of animal housing ... min) × t (min) (2) The conc of virus in impinger A for a given C s is calculated (3) The conc of virus in impinger B is calculated for the same C s in step (4) The volume sampled by both impingers...

Ngày tải lên: 12/08/2014, 04:20

... protein molecules and their interactions with DNA in real- time reactions and the dynamics of their DNA-binding Therefore, my objective of finding the characteristics of protein binding to single ... HU bind to DNA non-cooperatively causing DNA bending (20) at low protein binding density While at high protein binding density, IHF can crosslink DNA causing DNA condensation in the presence of ... is an NAP constituting 7–10% of the total protein in Mtb (78) It consists of 205 amino acid residues and contains large amounts of alanine (23.78% of total amino acids) and lysine (18.93%), with...

Ngày tải lên: 09/09/2015, 11:25

... borazine and benzene cyclacenes to obtain insights into the structural and electronic properties as a function of number of rings presented in cyclacenes In particular, we were interested in comparing ... 4k+2 in the peripheral circuit Two transannulene/annulenic circuits in the ring reveal interesting cryptoannulenic effect Fluorine (F) substitution increases the binding energy of the system in ... N-apexed and B-apexed borazine cyclacene with a more efficient delocalisation of electrons in the B-apexed ring Fluorination of borazine cyclacene results in an increase of bonding energy (BE) and ∆gap...

Ngày tải lên: 17/09/2015, 17:18

... lifetime instead of the values of lifetimes of the resolved components Binding of cAMP to anticAMP-binding sites leads to signicant changes in the uorescing properties of Trp13 of CRP(cAMP)2, including ... scattering measurements of the CRP DNA complex, which indicate that this structural change in the N-terminal domain of the protein occurs upon binding of DNA to the C-terminal domain of CRP [30] ... change in orientation of the DNA-binding domain relative to the cAMPbinding domain [6] Indeed, our Forster resonance ă energy transfer (FRET) measurements show that the binding of anticAMP in the...

Ngày tải lên: 07/03/2014, 16:20

... dynamics studies of ultrafast laser-induced nonthermal melting Fig Snapshots of atomic distributions of (a) laser heating with absorbed laser fluence of 13.1 J/m2 and (b) nonthermal melting triggered ... structure, since part of the energy deposited into the coherent phonons is converted to the kinetic energy of atoms However, we call this phenomena “nonthermal” melting in order to distinguish it ... in the ultrafast laser melting of silver [22] It Y Wang, X Xu Fig Temperature distribution in Bi2 Te3 thin film (a) Laser heating with absorbed laser fluence of 13 J/m2 and (b) nonthermal melting...

Ngày tải lên: 06/05/2014, 08:54

... apparent decrease in binding lifetimes (Fig 11B), suggesting that during the sliding the protein maintains registry with the DNA, and does not hop on and off, since the rate of re-binding to the DNA ... by avidin–biotin linkage while the other end was free in solution The protein was fluorescently labeled with Cy3B at the C-terminal engineered cysteine When these proteins were introduced into a ... We will discuss combing in more detail in section ‘‘Stretching by a moving interface’’ As mentioned in the section ‘‘Introduction,’’ stretching and immobilization of DNA using flow occurs either...

Ngày tải lên: 22/06/2014, 18:20

Ngày tải lên: 14/11/2014, 13:55

... (IE) of Ag atoms with respect to the case in Figure 1.1: A schematic diagram of the energy levels of diffused Ag atoms in pentacene thin film Ag diffusion induces hole doping in pentacene thin film ... effect of metal diffusion on the interfacial electronic structure of M/O interface by BEEM and in- situ charge transport measurements • to investigate the interplay between charge injection at M/O interface ... injection and in- situ charge transport measurements would yield information on the reliability of M/O interfaces under stressing and help in understanding the break-down mechanism of M/O interfaces...

Ngày tải lên: 11/09/2015, 08:59

... Smears were fixed in methanol for 10 minutes Immunofluorescence staining was carried out on the smears using pp65 staining kit obtained from Argene SA, France The smears were stained with mouse ... (64%) of the recipients were not As a result of the increased use of Gancyclovir prophylaxis, the incidence and severity of CMV disease is significantly reduced However, there is an increasing incidence ... value of real time PCR (qRT-PCR) with pp65 antigenemia The importance of correlating the test results with clinical symptoms is given Results A total of 57 donor recipient pairs were included in...

Ngày tải lên: 12/08/2014, 02:20