reaction restriction fragment length polymorphism analysis

... insertion segment IS900 by PCR (Sigurðardóttir et al 1999) IS900 restriction fragment length polymorphism analysis (RFLP) RFLP analysis was performed as described by Pavlik et al (1999) Briefly, ... Molecular epidemiology of Mycobacterium avium subsp paratuberculosis: IS900 restriction fragment length polymorphism and IS1311 polymorphism analyses of isolates from animals and a human in Australia ... (Stevensen et al 2002) Therefore, the potential of many different methods, such as IS900 restriction fragment length polymorphism (RFLP) (Whipple et al 1990, Collins et al 1990, Pavlik et al 1995), pulsed-field...

Ngày tải lên: 12/08/2014, 15:21

... 332 Sandeep Kumar Singh et al of M tuberculosis and M bovis Restriction fragment length polymorphism (RFLP) analysis based on IS6110 and IS1081 sequences easily and rapidly discriminates ... 1998, 4, 59-70 Das S, Paramasivan CN, Lowrie DB, Prabhakar R, Narayanan PR IS6110 restriction fragment length polymorphism typing of clinical isolates of Mycobacterium tuberculosis from patients ... Narayanan S, Sahadevan R, Narayanan PR, Krishnamurthy PV, Paramasivam CN, Prabhakar R Restriction fragment length polymorphism of M tuberculosis strains from various regions of India, using direct...

Ngày tải lên: 07/08/2014, 18:20

... kb), while a 2.5-kb restriction fragment Probe P8, one of the fragments adja- cent to P6 in Petunia, detected a BglII fragment similar to that of P6 in Q petraea samples (5-kb fragment) However, ... focus of our interest, as the most important oak species in Austria Direct DNA analysis by restriction frag- ment length polymorphism (RFLP), produces a theoretically infinite number of DNA markers ... did not show polymorphisms with the restriction enzymes used The polymorphism detected by the P6 Petunia probe with BglII had been described earlier (Kreike et al, 1991) The other polymorphisms...

Ngày tải lên: 08/08/2014, 19:21

... microarray data is unavailable [25] This method employs two restriction enzymes to generate short fragments in the analysis of AFLP The choice of restriction enzyme depends on the complexity of the target ... cropped gel fragments contained cDNAs of multiple genes (Additional file 1) These results had been expected, because the gel fragments included any cDNAs in the region Therefore, further analysis ... expression Figure RT-PCR analysis of the expression profile of the cDNA-AFLP-derived cDNA fragments ACAG2-1-, ACCT8-1-, ACCT2-1-, ACCT13-, and actin-specific RT-PCR analysis was carried out using...

Ngày tải lên: 11/08/2014, 11:21

... Thus, based on the analysis of this small portion of viral genome, subclade F1 is still present in São Paulo and roughly accounts for 4.05% of circulating strains Analysis of full -length subtype ... further phylogenetic analysis of the complete coding sequences and part of LTR region Of the 36 patients investigated, chimeric viruses comprised of B/F1 and F1/ C based on fragment analysis were detected ... designed the study, did the data analysis of the sequences, and wrote the first draft of the manuscript ÉRP conducted the characterization of the fulllength genome analysis WKN help with the laboratory...

Ngày tải lên: 12/08/2014, 04:21

... Keygene phát triển kỹ thuật AFLP ( Amplified Fragments Length Polymorphism) AFLP kết hợp RFLP (Restriction Fragments Length Polymorphism) PCR( polymerase chain reaction) đa hình đọan cắt RE khuếch đại ... http://www.ncbi.nlm.nih.gov/projects/genome/probe/doc/TechAFLP shtml http://doc.edu.vn/tai-lieu/de-tai-chi-thi-phan-tu-ky-thuat-aflp-amplified -fragment- lengthpolymorphisms-61035/ http://idoc.vn/tai-lieu/chi-thi-phan-tu-ky-thuat-aflp.html http://tailieu.vn/doc/ky-thuat-aflp-348752.html ... // -G MseI AATTC // T // -CTTAA G // AAT recognition sequences TAA // T // DNA restriction Chạy PCR với lọai mồi chọn lọc, mồi cấu tạo gồm phần: Trình tự nòng cốt( CORE), trình...

Ngày tải lên: 18/12/2014, 13:15

... Keygene phát triển kỹ thuật AFLP ( Amplified Fragments Length Polymorphism) AFLP kết hợp RFLP (Restriction Fragments Length Polymorphism) PCR( polymerase chain reaction) đa hình đọan cắt RE khuếch đại ... http://www.ncbi.nlm.nih.gov/projects/genome/probe/doc/TechAFLP.shtml http://doc.edu.vn/tai-lieu/de-tai-chi-thi-phan-tu-ky-thuat-aflp-amplified -fragment- lengthpolymorphisms-61035/ http://idoc.vn/tai-lieu/chi-thi-phan-tu-ky-thuat-aflp.html http://tailieu.vn/doc/ky-thuat-aflp-348752.html...

Ngày tải lên: 06/01/2015, 21:50

... biến đổi) chọn lọc dựa kiểu phép chọn lọc truyền thống thực Bằng kỹ thuật AFLP (amplified fragment length polymorphism) , phương pháp có hiệu để nhân dòng giải trình tự marker liên kết để xác định ... adapter primer dùng phân tích AFLP III KỸ THUẬT PCR (Polymerase Chain Reaction) Khái quát PCR chữ viết tắt cụm từ Polymerase Chain Reaction (Phản ứng chuỗi trùng hợp - phản ứng khuếch đại gen) Đây ... sử dụng lượng nhỏ DNA ban đầu (2.5pg-25ng) - Khuếch đại có chọn lọc lượng lớn đoạn DNA đa hình (polymorphism) phản ứng PCR, phù hợp cho việc phân tích đa dạng quần thể có quan hệ gần - Không cần...

Ngày tải lên: 21/07/2015, 15:36

... utilized to provide diagnosis of publication bias (Linear regression analysis, ref.[34] All analysis was done by using the Statistical Analysis System software (v.9.1.3, SAS Institute, Cary, NC) and ... meta -analysis study Thus, it is reasonable to hypothesize that the Arg72Pro polymorphism with reduced activity of p53 may play more important role in esophageal cancer risk In the present meta -analysis ... more than 95% of esophageal malignancies [48] In our meta -analysis, we had wanted to analysis the association between these two gene polymorphisms and risk of esophageal cancer according to the...

Ngày tải lên: 25/10/2012, 11:40

... utilized to provide diagnosis of publication bias (Linear regression analysis, ref [21]) All analysis was done by using the Statistical Analysis System software (v.9.1.3, SAS Institute, Cary, NC) and ... that the -28C/G polymorphism with higher activity of RANTES may play more important role in asthma risk In the present meta -analysis on the association between RANTES -28C/G polymorphism and ... association between RANTES -28C/G polymorphism and asthma risk Human studies, regardless of sample size, were included if they met the following criteria: (1) RANTES polymorphism at -28C/G were determined...

Ngày tải lên: 26/10/2012, 09:39

... by HPLC analysis (data not shown) Notably, the catalytic fragment is still active at a temperature of 30 C, which is important with regard to our approach to investigate the molecular reaction ... resolved by global t analysis and reect the formation and decay of a single observable reaction intermediate Given the many intermediates that have been recognized during the reaction mechanism ... order to study the reaction mechanism of the ATPase, we explored here whether a truncated variant of CopB could act as model for the holoenzyme Therefore, the soluble catalytic fragment CopB-B,...

Ngày tải lên: 07/03/2014, 00:20

... substrate ATP The analysis of mutant variants of HypE demonstrates that the carbamoylation of the C-terminal thiol and the dehydration of the S-carboxamide to the thiocyanate are independent reactions ... reactions and that HypE per se is able to catalyze the dehydration reaction In addition, it is shown that during the carbamoyltransfer reaction HypE forms a complex with the HypF protein The results ... excised from plasmid pHypED by restriction with MluI, treatment with Klenow enzyme and SpeI digestion and cloned into plasmid pTE-C2 via replacement of a HindIII -fragment that was treated with...

Ngày tải lên: 16/03/2014, 18:20

... strand-exchange reaction, because the reaction only depended on the DMC1 concentration (Fig 5, lanes 1–6) Similarly, the conventional DMC1 protein did not affect the DMC1-I37N-mediated strand-exchange 460 reaction ... were added to the reaction mixture, which was incubated at 37 °C for 10 The reactions were then initiated by the addition of 20 lm /X174 linear dsDNA, and were continued for h The reactions were ... Science; ATP sodium salt), the reaction was initiated by adding mm ATP At the indicated times, 20 lL of the reaction mixture was mixed with 30 lL of 100 mm EDTA to quench the reaction The amount of inorganic...

Ngày tải lên: 23/03/2014, 06:20

... of Participation Restriction appeared to have acceptable properties The item analysis resulted in the removal of Impairment items, Activity Limitation items and 11 Participation Restriction items ... redundant or misfitting items In an item analysis, the candidate items are completed by participants from the target population and analysed statistically This analysis can suggest items that may ... to item analysis is based on correlational data and the procedures usually involve maximising Cronbach's alpha [12] and selecting items with high factor loadings using exploratory factor analysis...

Ngày tải lên: 18/06/2014, 18:20

... much greater with the intact antibody than it is for the fragment The Fab fragments, smaller in size, clear even faster than an F(ab’)2 fragment and have been developed predominantly as imaging ... reports utilizing a fragment of panitumumab for either imaging or therapeutic applications This paper represents the first in vitro and in vivo characterization of panitumumab F(ab’) fragment with ... using a BSA standard [28] and the product was analyzed by SDS-PAGE Upon completion of the analysis, F(ab’)2 fragments were then prepared from g of panitumumab In this situation, a tangential flow...

Ngày tải lên: 21/06/2014, 02:20

... balances In this work, a reaction calorimeter containing a homogeneous reaction mixture was employed To describe the development of heat fluxes due to reaction in the reaction calorimeter, a mathematical ... heat & fluxes here are the heat flux of the reaction, Q reaction, and the heat flux of cooling, & Q cooling They are defined as: & Qreaction = ΔH reaction rVr (10) & Qcooling = UA(Tr − T j ) ... used to calculate the reaction rate, r, using the same kinetics used to solve the mass balance The reaction rate is then used to calculate the & heat flux of reaction, Q reaction, according to...

Ngày tải lên: 22/07/2014, 03:20

... [27] emphasised that crown length is an important parameter which is related to a tree’s diameter growth and length of the clear bole Nevertheless, our knowledge on crown length estimation is restricted ... wide range of crown lengths (Tab I) The sampled trees were from different sociological positions, including codominant and dominated trees All sample trees were felled and stem analysis was conducted ... branch death see Tab III and Sect methods) 2.5 Design of branch whorl analysis A precise reconstruction of a tree’s crown length requires the examination of each whorl in the dead branch zone...

Ngày tải lên: 08/08/2014, 01:21

... This method was chosen because it is more precise than HPLC analysis for the measurement of the average molecular weight of HMW hyaluronan Analysis of message expression by quantitative realtime ... control value) (Fig 1) Expression profile of hyaluronidase isozyme messages Statistical analysis Statistical analysis was with Wilcoxon's matched-pairs signed rank test A probability value of

Ngày tải lên: 09/08/2014, 01:24

... Đoàn Bộ và nnk. / Tạp chí Khoa học ĐHQGHN, Khoa học Tự nhiên và Công nghệ 26, Số 3S (2010) 295‐301 301 Using the Length- Based Cohort Analysis Model (LCA) to study ocean large pelagic fish resources and fishery management ... on some species of ocean large pelagic fish in offshore waters of Vietnam Centre, the Length- based Cohort Analysis and Thompson & Bell prediction models have been used The obtained results showed...

Ngày tải lên: 09/08/2014, 17:23