... Oxidative half -reaction of wild-type and variants of DAAO.(A) Comparison of time courses of the (re)oxidation of reducedwild-type and W50 variants of DAAO followed at 455 nm upon mix-ing of 10 ... contributes to the d-amino acid preference of yeast DAAO.Kinetic mechanism of DAAO variantsSteady-state kineticsDependence of the catalytic activity of DAAO variantson the oxygen and d -alanine concentrations ... half -reaction of wild-type and variants of DAAO.Comparison of time courses of flavin reduction followed at 455 nm(vertical bars = experimental data points) for W50 variants (A) and T201L ⁄ V and...
... consistency and texture of fermented food. One hundred and fifteenstrains of Lactic Acid Bacteria (LAB) were isolated and characterized from some fermented dairy (“Nono”,“Fura”, Yogurt, “Wara”) and ... their biosynthesis and molecular organization and mainly because of their GRAS (generally regarded as safe) fermentation strategy is rather scarce and the kinetics of status [1] and their rheological ... 10ml of mESM broth and microbiological analysis. the broths were incubated anaerobically for 24h at 30°C.Isolation of lactic acid bacteria: Serial dilutions of flasks containing 90 ml of mESM...
... Dental Sciences and Surgery, University of Bari, Bari, Italy. Correspondence to: Prof. F.R. GRASSI, Professor and Dean, Department of Dental Sciences and Surgery - University of Bari, P.zza ... Larsson A, Welander U, Frithiof L, Engstrom GN. The effect of hyaluronan on bone and soft tissue and immune response in wound healing. J Perio-dontol. 2001 Sep; 72(9):1192-200. Tables and Figures ... and other tissues. HA structure consists of polyanionic disaccharide units of glucouronic acidand N-acetyl-glucosamine con-nected by alternating β 1–3 and β1–4 bonds [1]. There is no anti-genic...
... findings of nonedetectable. The authors also reported 101 ( 38 and 22.1 ( 15.2àg -carotene/g FW in extracted samples of aril and mesocarp,respectively, and 16 and 9 àg/g of zeaxanthin and 35 and ... identification of major carotenoids, includes carotenoidprofiles, measuring both trans- and cis-isomers of lycopene and β-carotene. We also provide a detailed fatty acid analysis of gac seed and aril, ... linoleic and R-linoleic acids.The aril also contains a significant, but varying, amount of stearic acidand small amounts of cis-vaccenic, myristic, eicosa-11-enoic, arachidic, and palmitoleic acids.Our...
... substrate specificity of Table 2. Kinetic parameters for the reductive and oxidative half-reactions of wild-type, H181A, H184A and Y186F PETN reductases. Kineticdata are shown in Figs 3 and 4. Parameters ... architec-ture of OYE1 and PETN reductase, and (ii) geometryfor the binding of steroid compounds and 2-cyclohe-xen-1-one [2,19]. The structure of the Y186F enzyme isessentially identical to that of wild-type ... 2005)doi:10.1111/j.1742-4658.2005.04875.xThe roles of His181, His184 and Tyr186 in PETN reductase have beenexamined by mutagenesis, spectroscopic and stopped-flow kinetics, and bydetermination of crystallographic structures...
... length and orientation of helices. The superposition of the IPDC monomer on the subunit of ScPDC and ZmPDCresults in rmsd of 1.24 A˚for 470 out of 563 Ca atoms and 1.48 A˚for 496 out of 568 ... fromL-trypto-phan, and consists of three steps: (a) the conversion of tryptophan to indole-3-pyruvic acid; (b) the formation of indole-3-acetaldehyde; and (c) the production of IAA(Fig. 1). The first step of ... structure elements of IPDC are shown inFig. 4, together with the aligned amino acid sequences of IPDC and ZmPDC. The topology of the IPDC monomer issimilar to that of ScPDC and ZmPDC with some...
... producingtransformant and was used for the purification of GALA.Growth of this transformant and subsequent purification of the enzyme were performed as described in Materials and methods. Purity of the preparation ... Purification and properties of a-L-arabinofuranosidase and endo-b-D-1,4-galactanase from Aspergillus niger v. TieghemKF-267 which liquified the Okara. In Genetics, Biochemistry and Ecology of Cellulose ... to facilitate purification of GALA. The enzyme has a molecular mass of 48.5 kDa and a pH optimum between 4 and 4.5. Incubations of ara-binogalactans of potato, onion and soy with GALA resultedinitially...
... thebasis of the kinetic constants in Table 1, and those obtainedfor the glutaminase reaction in the presence of 0.1 mMeach of UTP and ATP-cS (data not shown). Apparently, thevalue of Ka, ... of nucleotides (Table 1).The rate of CTP synthesis was dramatically influenced bythe presence of GTP, and 20 and 41-fold increases in kcatwere obtained with GTP concentrations of 0.1 mM and 1mM, ... activation of theglutaminase activity in the presence of 0.1 mM(open circles) or 1 mM(closedcircles)eachofUTPandATP-cS. (C) GTP activation of CTPsynthesis at 0.1 mMeach of UTP and ATP.4776...
... results of a steady-state kineticanalysis of DptE. The aim of this kinetic study was todetermine the specificity of DptE for various fattyacids and noncognate ACPs. The Michaelis–Mentenkinetic ... chain fatty acids and Table 2. Kinetic parameters for steady-state analysis of the DptE-catalysed lipidation of DptF determined at varying concentrations of fatty acids or DptF, LipD and AcpK.Substrate ... fattyacids naturally found in A21987C. Hexanoic acid, palmitic acidand 15-methylhexadecanoic acid were notaccepted by DptE.In summary, DptE is capable of transferring avariety of fatty acids...
... protection of enzymes and membranes from thedetergent effects of excess fatty acids by sequesteringthem, and (d) modulation of cell growth and differenti-ation by transport of fatty acids to the nucleus ... uptake of fattyacids across the plasma membrane and transport tovarious subcellular organelles, (b) modulation of theactivity of enzymes involved in fatty acid metabolism,(c) protection of enzymes ... consists of four exons of 112, 174, 89 and 141 bp, coding for 22, 59, 30 and 20amino acids, respectively. The intron ⁄ exon structure of the fabp6 gene from zebrafish is consistent with that of all...