radiometric system indirect susceptibility testing for mycobacterium tuberculosis
Characterisation of toll like receptor 8 as a new innate immune receptor for mycobacterium tuberculosis
... Prof!Ng!Mah!Lee!and!Yap!Han !for! electron!microscopy!work;!!Prof!Mike!Kemeny! and!Christopher!Yang! !for! cloning!the!TLR8.2!receptor;!and!A/Prof!Lu!Jinhua!and! Jason!Goh !for! the!TLR8!WT!plasmid!and!techniques !for! the!dual!luciferase!assay.!! ... Buffers !for! Cell!Culture!and!Isolation!of!Primary!Cells 40! 2.1.2! Buffers !for! Mycobacteria!Culture!and!Work 40! 2.1.3! Buffers !for! SDS.PAGE!and!Western!Blot 41! 2.1.4! Buffers !for! DNA!Gel!Electrophoresis ... potential against Mycobacterium- tuberculosis. ! (Manuscript! in!preparation)! ! ! xvii! ! Chapter.1 Introduction ! 1! ! ! 1.1 Chapter!1:!Introduction! ! Mycobacterium- tuberculosis 1.1.1...
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Tài liệu Testing for tuberculosis pptx
... investigation for active pulmonary tuberculosis They are mainly used for detecting latent tuberculosis in Latent infection people when active tuberculosis has been excluded, and for Screening for latent tuberculosis ... pulmonary tuberculosis Screening for latent tuberculosis infection The Australian National Tuberculosis Advisory Committee recommends tuberculin skin testing as the standard test for latent tuberculosis ... responsible for transmission of tuberculosis Other testing tuberculosis remain the most effective strategies for public (including further medical imaging, immunological tests or health control of tuberculosis...
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Guidelines for Preventing the Transmission of Mycobacterium tuberculosis in Health-Care Settings, 2005 pot
... After baseline testing for infection with M tuberculosis, HCWs should receive TB screening annually (i.e., symptom screen for all HCWs and testing for infection with M tuberculosis for HCWs with ... consists of four major components: 1) baseline testing for M tuberculosis infection, 2) serial testing for M tuberculosis infection, 3) serial screening for symptoms or signs of TB disease, and 4) ... tuberculosis are available (30,31,39) Baseline Testing for M tuberculosis Infection Baseline testing for M tuberculosis infection is recommended for all newly hired HCWs, regardless of the risk...
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Báo cáo khoa học: Mycobacterium tuberculosis possesses a functional enzyme for the synthesis of vitamin C, L-gulono-1,4-lactone dehydrogenase doc
... UDPglucose dehydrogenase Mycobacterium tuberculosis possesses a gene encoding a putative UDP-glucose dehydrogenase (Rv0322) that is necessary for UDPglucuronic acid formation Moreover, the pathogen ... physiologic role of the Rv1771 protein in M tuberculosis? Mycobacterium tuberculosis Fig Sequence relationship between L-gulono-1,4-lactone dehydrogenase of M tuberculosis and previously identified ... stages of M tuberculosis infection Interestingly, the promoter of the cell wall catalase-peroxidase ⁄ NADH oxidase [43] gene katG of M tuberculosis, which is important for virulence and for the activation...
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Báo cáo khoa học: Adenylyl cyclase Rv0386 from Mycobacterium tuberculosis H37Rv uses a novel mode for substrate selection ppt
... cyclases in Mycobacterium tuberculosis H37Rv: cloning and characterization J Biol Chem 276, 35141–35149 17 Linder JU, Schultz A & Schultz JE (2002) Adenylyl cyclase Rv1264 from Mycobacterium tuberculosis ... classification of cNMP-binding proteins and nucleotide cyclases with implications for novel regulatory pathways in Mycobacterium tuberculosis Genome Res 10, 204–219 16 Reddy SK, Kamireddi M, Dhanireddy ... Adenylyl cyclase Rv1625c of Mycobacterium tuberculosis: a progenitor of mammalian adenylyl cyclases EMBO J 20, 3667–3675 FEBS Journal 272 (2005) 3085–3092 ª 2005 FEBS M tuberculosis adenylyl cyclase...
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CLSI 2013: performance standard for antimicrobial susceptibility testing 2013
... (AST) systems is found in Table 3C for disk diffusion and Table 4F for MIC testing Guidance for troubleshooting out-of-range results is addressed in Table 3D for disks and Table 4G for MIC testing ... M45—Methods for Antimicrobial Dilution and Disk Susceptibility Testing of Infrequently Isolated or Fastidious Bacteria provides suggestions for standardized methods for susceptibility testing, including ... ranges for QC strains are provided in Tables 3A and 3B for disk diffusion and Tables 4A through 4E for MIC testing Guidance for frequency of QC and modifications of antimicrobial susceptibility testing...
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Báo cáo y học: " Theoretical basis for reducing time-lines to the determination of positive Mycobacterium tuberculosis cultures using thymidylate kinase (TMK) assays" ppt
... identification of Mycobacterium tuberculosis (M.tb) cultures There are several existing methods for detecting TB within cultures, but they mostly lack inexpensive platforms for routine use within ... still form the most reliable and readily affordable method for diagnosing TB in many resourcelimited settings [10] In general, in vitro culture of pathogens on appropriate growth media forms ... Infection with Mycobacterium tuberculosis (M.tb), the causative agent of tuberculosis (TB), is one of the leading global health challenges [1,2] An estimated 8–10 million persons acquire tuberculosis...
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Tiểu luận:tiểu luận Vacxin phòng bệnh do vi khuẩn Mycobacterium tuberculosis
... vật ký chủ Vi khuẩn Mycobacteriumtuberculosis gh Vi khuẩn Mycobacteriumtuberculosis Khuẩn lạc vi khuẩn Mycobacteriumtuberculosis Phân loại : Phức hợp M tuberculosis gồm loài Mycobacterium khác ... đặc Bệnh vi khuẩn Mycobacterium tuberculosis: - Gồm loại vi khuẩn lao là: vi khuẩn lao người (Mycobacterium tuberculosis) Vi khuẩn lao bò (Mycobacterium bovis) Vi khuẩn lao chim (Mycobacterium avium) ... bệnh nhân lao mạn tính II Tổng quan : Mycobacteriumtuberculosis (MBT): - Vào năm 1882, nhà vi sinh học người Đức Robert Koch phát khuẩn que u lao (Mycobacterium tuberculosis) , tác nhân gây bệnh lao...
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Tài liệu Testing for No Records docx
... Test Orders DataReader for records result.Append("DataReader " + ORDERS_TABLE + ": Has records = " + orderReader.HasRows + Environment.NewLine); // Test Orders DataReader for records bool readerHasRecords ... // Test Orders DataTable for records bool tableHasRecords = orderTable.Rows.Count > 0; result.Append("DataTable " + ORDERS_TABLE ... DataRowCollection has a Count property that returns the number of rows in the table The Count property for an empty table has a value of The HasRows property of the DataReader returns a Boolean value...
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Tài liệu Fiber Managment System Delivers Quick ROI for Central Office doc
... a best practices initiative for long-term reliability and service availability Before the project, just having long fiber jumpers around the CO was an invitation for technicians to use them by ... rearrangements neither taxed manpower nor presented serious issues for customers However, with introduction of new high-speed, high-density fiber platforms, fiber cable congestion was starting to become an ... CO personnel decided to proactively attack the issue by designing a standard fiber management system for the CO The objectives were to reduce trouble-tracing time, improve restoration time, and...
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Tài liệu Báo cáo " Development of system of HydrodynamicEnvironmental models for coastal area (Case study in Quang Ninh - Hai Phong region) " doc
... Model for suspended particulate matter transport and bottom bathymetry evolution The model for SPM and bottom bathymetry evolution includes sub-models: 3D SPM Model for water column and 2D model for ... we need to build a system of models for each environment which is related to each other through the boundary conditions In this case, we can introduce the system of models for oil slick, oil-in-water ... wind Conclusion The preliminary results for the combined system of 2D, 3D hydrodynamicenvironmental models for SPM and bottom layer change show the ability to forecast the fate and transport of contaminants...
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Tài liệu Antimicrobial Susceptibility Testing Protocols ppt
... broth For fastidious animal pathogens For fastidious animal pathogens For dilution tests of anaerobes For dilution tests of anaerobes For dilution tests of anaerobes For dilution tests of yeasts For ... funigatus (204305) For dilution and disk tests For β-lactam/inhibitor combinations For ESBL screening tests For dilution and disk tests, especially for aminoglycosides vs P aeruginosa For dilution ... Institute Performance standards for antimicrobial susceptibility testing, informational supplement M100-S16, 16th ed., CLSI, Wayne, PA, 2006 2 Antimicrobial Classifications: Drugs for Bugs Cassandra...
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Tài liệu Báo cáo khoa học: Survival mechanisms of pathogenic Mycobacterium tuberculosis H37Rv ppt
... of isocitrate lyase in Mycobacterium avium and Mycobacterium tuberculosis J Bacteriol 181, 7161– 7167 Graham JE & Clark-Curtiss JE (1999) Identification of Mycobacterium tuberculosis RNAs synthesized ... virulence of Mycobacterium tuberculosis Microb Pathog 45, 12–17 ´ 79 Perez E, Samper S, Bordas Y, Guilhot C, Gicquel B & Martı´ n C (2001) An essential role for phoP in Mycobacterium tuberculosis ... & Ratledge C (1994) Metabolism of Mycobacterium tuberculosis In Tuberculosis: Pathogenesis, Protection and Control (Bloom B ed), pp 353–385 American Society for Microbiology, Washington, DC Voss...
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Tài liệu A New Banking System The Needful Capital for Rebuilding the Burnt District docx
... under this system, make no absolute promise to pay specie on demand, the system nevertheless affords a much better practical guaranty for specie payments, than the old specie paying system (so ... natural rights, for a government to forbid the lending and hiring of money for manufacturing industry, as it is to forbid the lending and hiring of land, or agricultural implements, for agricultural ... IV SECURITY OF THE SYSTEM Supposing the property mortgaged to be ample, the system, as a system, is absolutely secure The currency would be absolutely incapable of insolvency; for there could never...
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Tài liệu Báo cáo khoa học: Studies on structural and functional divergence among seven WhiB proteins of Mycobacterium tuberculosis H37Rv pdf
... 0.05 were selected for the cross-reactivity assay and were as follows: : 35 000 for WhiB1; : 25 000 for WhiB2; : 20 000 for WhiB3, WhiB4 and WhiB5; : 15 000 for WhiB6 and : 50 000 for WhiB7 The activity ... properties of M tuberculosis WhiB proteins as follows: 17 550 m)1Æcm)1 for WhiB1, 13 140 m)1Æcm)1 for WhiB2, 18 830 m)1Æcm)1 for WhiB3 and WhiB4, 16 980 m)1Æcm)1 for WhiB5, 27 200 m)1Æcm)1 for WhiB6 ... induced by the addition of 0.3 mm isopropyl thio-b-d-galactoside for 18–20 h at 16 °C (for WhiB proteins) or for h at 30 °C (for IscS) Cells from 1000 mL culture were re-suspended in 10 mL buffer...
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Tài liệu Báo cáo khoa học: The Mycobacterium tuberculosis membrane protein Rv2560 ) biochemical and functional studies pdf
... synthesis was observed in the following strains: M tuberculosis H37Rv, M tuberculosis H37Ra, Mycobacterium bovis, M bovis BCG, Mycobacterium africanum and Mycobacterium microti (Fig 1A, lanes 2–7) tblastn ... were carried out after DNA had been denatured for at 94 °C: 56 °C for 30 s, 72 °C for 40 s and 95 °C for 40 s A final extension cycle was then performed at 72 °C The amplification products were ... antibiotics were preincubated for h in the presence of 50, 100 or 200 lm HABP 11024 for U937 cells and 100)105 nm or 200 lm HABP 11024 for A549 cells before the addition of · 107 M tuberculosis H37Rv bacilli...
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Tài liệu Báo cáo Y học: Evidence that a eukaryotic-type serine/threonine protein kinase from Mycobacterium tuberculosis regulates morphological changes associated with cell division docx
... (denaturation, 95 °C for 30 s per cycle; annealing, 50 °C for 30 s per cycle; elongation, 68 °C for for first 10 cycles and then for the remaining 20 cycles the elongation step was extended for an additional ... obtained was used for PCR amplification of pknA The Expand Long Template PCR system (mixture of Pwo and Taq DNA polymerases; Roche Molecular Biochemicals) was used for this purpose The forward (CC7: ... of Mycobacterium tuberculosis: characterization and localization Microbiology 147, 2307–2314 17 Av-Gay, Y & Everett, M (2000) The eukaryotic-like ser/thr protein kinases of Mycobacterium tuberculosis...
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Tiểu luận:Vacxin phòng bệnh do vi khuẩn Mycobacterium tuberculosis doc
... vật ký chủ Vi khuẩn Mycobacteriumtuberculosis gh Vi khuẩn Mycobacteriumtuberculosis Khuẩn lạc vi khuẩn Mycobacteriumtuberculosis Phân loại : Phức hợp M tuberculosis gồm loài Mycobacterium khác ... đặc Bệnh vi khuẩn Mycobacterium tuberculosis: - Gồm loại vi khuẩn lao là: vi khuẩn lao người (Mycobacterium tuberculosis) Vi khuẩn lao bò (Mycobacterium bovis) Vi khuẩn lao chim (Mycobacterium avium) ... bệnh nhân lao mạn tính II Tổng quan : Mycobacteriumtuberculosis (MBT): - Vào năm 1882, nhà vi sinh học người Đức Robert Koch phát khuẩn que u lao (Mycobacterium tuberculosis) , tác nhân gây bệnh lao...
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Báo cáo khoa học: A novel inhibitor of indole-3-glycerol phosphate synthase with activity against multidrug-resistant Mycobacterium tuberculosis pptx
... rapid radiometric method for drug susceptibility testing of Mycobacterium tuberculosis J Clin Microbiol 13, 908–912 35 Bradford MM, McRorie RA & Williams WL (1976) A rapid and sensitive method for ... (http://pymol.sourceforge.net) and vmd [29] M tuberculosis H37Rv, M tuberculosis H37Ra and clinical isolates of M tuberculosis were provided by Shanghai Pulmonary Hospital of China M tuberculosis and Mycobacterium ... (lgÆmL)1) M tuberculosis H37Ra M tuberculosis H37Rv M bovis BCG 1 0.10 0.10 0.10 Table Susceptibility of M tuberculosis clinical isolates to ATB107 measured by the BACTEC radiometric system The...
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Báo cáo khoa học: Kinetic and mechanistic characterization of Mycobacterium tuberculosis glutamyl–tRNA synthetase and determination of its oligomeric structure in solution pptx
... biosynthesis are essential for M tuberculosis For these reasons, with the dual goal of contributing to understanding of the metabolism of this pathogen and providing the enzyme for the identication ... encoding the putative M tuberculosis GluTR (Mt-GluTR) was also cloned in vectors for protein production in E coli in order to ask questions about GluRSGluTR complex formation for the mycobacterial ... number is similar to that reported for Ec-GluRS, as are the KM values for ATP and tRNAGlu However, KL-Glu was found to be $ 20fold higher for Mt-GluRS than for the E coli enzyme Such a difference...
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