r l stine the haunting hour afraid of clowns

... the marks of nonhuman (alien, artificially intelli- gent, or supernatural) design (Ratzsch 2001, 118–20). This is the premise of the Search for Extra Terrestrial Intelligence (SETI), of research ... supposing that the complete history of a football team starts with the moment that the coach gathers together the players, thereby ignoring the important work the players had already done. Before Johnson ... practice. For Rea, the problem is that naturalism can- not explain the modal qualities of particular physical objects. For Koons, the problem is that naturalism cannot account for the reliability...

Ngày tải lên: 01/11/2013, 08:20

... heterologous host. The encoded proteins were purified and their kinetic properties characterized. GLD1 catalyses the conversion of d-glyceralde- hyde and l- glyceraldehyde to glycerol, whereas GLD2 ... activity with C5 or C6 sugars. We tested d-glyceraldehyde and l- glyceraldehyde individually and observed similar activities; the activity with l- glyceraldehyde was only slightly lower. For d-glyceraldehyde ... enzymes were purified and characterized. We show that one enzyme catalyses the reduction of d-glyceraldehyde and l- glyceraldehyde to glycerol, whereas the other reduces DHA. This is the first report on...

Ngày tải lên: 19/02/2014, 06:20

... few other short stretches, the structures were all very similar over the entire polypeptide chain. However, the presence of the ligands significantly reduced the length of the disordered regions: ... site residues (including Arg223 and Ser227), either directly or indirectly. Similarly, the 33 ordered residues in the T structure (that are disordered in the N form) are not related to the Tris-binding ... unified set of procedure for evaluating the quality of macromolecular structure-factor data and their agreement with atomic model. Acta Crystallogr D Biol Crystallogr 55, 191–205. 42 Krissinel E &...

Ngày tải lên: 06/03/2014, 09:22

... found in the cyto- plasm a nd extracellular matrix where they may have regulatory functions, unlike in somatic cells of multicellular organisms where these enzymes are primarily lysosomal and thought ... represent the pI values of the major bands in e and f, while the numbers at the left are the pI values of standard proteins. The arrow at the right represents the pI value of 6.84 calculated for ... Biological Sciences, University of Windsor, Windsor, Ontario, Canada; 2 Department of Molecular Cell Biology, Sylvius Laboratory, Leiden, the Netherlands We reported previously that the major cysteine...

Ngày tải lên: 07/03/2014, 16:20

... b-strand (A) parallel to the first b-strand, B, of the jelly roll core. The linkage of the fourth (E) and fifth (F) b-strand of the jelly roll fold is built up by an extended insert (Val199–Leu296) consisting ... auto- mated program for molecular replacement. J Appl Crystallogr 30, 1022–1025. 38 Emsley P & Cowtan K (2004) Coot: model-building tools for molecular graphics. Acta Crystallogr D Biol Crystallogr ... form a hydrogen bond and salt bridge with the a-amino group, although the carboxyl group of Glu170 also coordinates the catalytic iron. Furthermore, the carboxyl group of l- arginine forms a salt...

Ngày tải lên: 16/03/2014, 02:20

... sugar-binding site because of the num- ber of intermolecular hydrogen bonds and of residues interacting with lactose. Our results agreed well with those from the crystal structure of the complex between ... 6108–6117. 53 Koradi R, Billeter M & Wu ¨ thrich K (1996) MOLMOL: a program for display and analysis of macromolecule structures. J Mol Graphics 14, 51–55. Supporting information The following supplementary ... aggregates of glycopro- tein and glycolipid receptors. Thus, the carbohydrate cross-linking properties of lectins are a key feature of their biological activities [30,43,44]. R- type lectins are reported...

Ngày tải lên: 23/03/2014, 04:21

... this report include those funding as sequestrable discretionary resources. That is because the STA requires for purposes of this report that OMB assume all discretionary appropriations are repeated ... 251A(5), the total reduction of $54.667 billion is allocated pro- portionately between discretionary appropriations and direct spending. The total base is the sum of the FY 2013 revised discretionary ... and classifications in the report are preliminary. If the sequestration were to occur, the actual results would differ based on changes in law and ongoing legal, budgetary, and technical analysis....

Ngày tải lên: 23/03/2014, 23:21

... harvested before the sugars were utilized, and the crude cell extract was analyzed for l- rhamnose dehydrogenase activity. Cells grown on l- rhamnose as a sole carbon source had an l- rhamnose dehydrogenase ... Marechal LR & Veiga LA (1979) Induction and catabolite repression of L- rham- nose dehydrogenase in Pullularia pullulans. J Bacteriol 138, 55–59. 12 Jeffries TW, Grigoriev IV, Grimwood J, Laplaza ... fractions of plant biomass, such as hemicellulose and pectin. Several microorganisms liv- ing on decaying plant material are able to use l- rham- nose as a source of carbon and energy. There are...

Ngày tải lên: 30/03/2014, 04:20

... of extracellular CP may be favored by the increased expression of vacuolar-type H + -ATPase components, which reduces the pH of the pericellular environment of macrophages [20]. This raises the ... France). N a -benzoyl- L -arginine-4-nitroanilide ( L -BAPA) was from Merck KgaA (Darmstadt, Germany). E-64 and phenyl- methanesulfonylfluoride were from Sigma-Aldrich (St Quentin le Fallavier, France). Molecular ... National Institute of Health grant, AR46182. C. D. holds a doctoral fellowship from MENRT (Ministe ` re de l Education Nationale, de la Recherche et de la Technologie, France). References 1. Mu ¨ ller-Esterl,...

Ngày tải lên: 31/03/2014, 07:20

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Ngày tải lên: 02/04/2014, 14:43