quantitative characterization of biological tissue using optical spectroscopy

... and that mechanical characterization of protein materials may allow for gaining insight into the biological functions of mechanical proteins Mechanical characterization of biological molecules ... we present the mesoscopic model of biological protein materials composed of protein crystals prescribed by Go potential for characterization of elastic behavior of protein materials Specifically, ... mechanical properties of biological materials There have been few literatures26-28 on the characterization of macroscopic mechanical properties such as Young’s modulus of biological materials...

Ngày tải lên: 22/02/2014, 08:20

... Therefore, further reduction of the reflectance will be expected from the optimization of the synthesis conditions of SiNWs For analysis of the electrical properties, the I–V curve of the fabricated SiNWs ... FE-SEM image of the phosphorous-diffused SiNWs The shapes of SiNWs after formation of pn junction were slightly curved As mentioned above, we expect the SiNWs solar cells to show improved optical ... at λ b 700 nm [20] The difference of the reflectance might be the effect of synthesis conditions, such as thickness of metal film and SiH4 gas flow The reduction of reflectance is very helpful to...

Ngày tải lên: 16/03/2014, 15:12

... concentration of approximately 20 to 50 ng of DNA per μl); pmol of each primer (Microsynth, Switzerland) (list of primers used are given in Table 1); 500 μM each of dNTPs (Sigma-Aldrich, USA); 0.7 unit of ... results of the microsatellite analysis in terms of number Genetic characterization of Barbari goats 75 of alleles observed, alleles size, polymorphism information content and heterozygosity of Barbari ... for molecular characterization of goat germplasm In general, the PIC values are suggestive of high polymorphic nature of the microsatellite loci analysed It refers to the value of a marker for...

Ngày tải lên: 07/08/2014, 23:22

... construction of the biological system model on the 'model canvas' (Figure 2a) A MetaReg model consists of a set of biological variables and their regulatory logics The 'variables' represent different biological ... minutes of incubation and in amino acid deprivation after 1, 1.5 and hours of incubation [16]; six profiles in histidine starvation and various Gcn4 perturbations [17]; six profiles of chemostat ... and six profiles in nitrogen depletion after 8, 12 and 24 hours of treatment and in amino acid and adenine starvation after 1, and hours of treatment [19] A complete description of the profiles,...

Ngày tải lên: 14/08/2014, 08:20

... 153] 26 E Optical clearing The application of optical clearing methods has been shown to decrease tissue scattering, increasing optical transmittance [146, 154-158] The application of optical clearing ... in three dimensions in the context of tissues and organs in living animals In brain tissue, the first tissue to be studied using MPM, images were collected of neurons in invertebrate ganglia, ... deep tissue imaging MPM utilizes near-infrared wavelengths of light (700-1000 nm) for excitation These wavelengths are within an optical window” in the absorption spectrum of biological tissue, ...

Ngày tải lên: 24/08/2014, 13:39

Ngày tải lên: 04/10/2014, 22:10

... and progenitor properties of these CIC [42] Table 2: List of various types of cancers in which cancer initiating cells are isolated using marker profiles Table 3: List of various cancer cell lines ... Schematic of the PM-SHIM set up Figure 16: Chirp analyses of laser beam of the PM-SHIM shows a distinct temporal profile improvement after AOM Figure 17: Chirp analyses of the laser beam of the ... and spreading of tumor A quantitative observation and characterization of this ECM remodeling especially in the case of side population will give us useful insights into the process of tumor progression...

Ngày tải lên: 14/09/2015, 08:42

... enormous amount of credit belongs to a multitude of people for making this research successful First of all, I would like to thank my supervisor, Prof Chan Sze On Hardy of Department of Chemistry ... mechanism of the PANI-CS nanoparticle41 2.3.3 Effect of reaction parameters on the size of PANI-CS nanoparticles… 47 2.3.4 Effect of reaction parameters on the colloidal stability of PANI-CS ... Formation Mechanism of PPy-CS nanoparticles….67 3.3.3 Effect of Reaction Parameters on the Size of PPy-CS Nanoparticles….73 3.3.4 Effect of Reaction Parameters on the Colloidal Stability of PPy-CS Dispersion………………………………………………………………74...

Ngày tải lên: 15/09/2015, 17:10

... power of the emitters, sensitivity of the detector, type of modulation code and bit error rate (BER) can influence the optical quality of the signal, in terms of optical modulation amplitude (OMA) ... value of P0 at first output We can conclude that in spite of using off-the-shelf LCs after a correct optimization and without the need of precise systems of alignment, the limiting factor in the optical ... increased to the overload level of the receiver Optical modulation amplitude of the system The power of logic can be decreased by using return-to-zero (RZ) signal instead of nonreturn-to-zero (NRZ)...

Ngày tải lên: 22/06/2014, 22:20

... facilities to carry out the research work i Table of Contents Acknowledgement Table of Contents Summary List of Tables List of Figures List of Equations List of Symbols and Abbreviations Chapter Introduction ... focusing on the development of novel sensing strategies and the improvement of specificity, sensitivity, and response time Optical methods which have been widely used in biosensors include spectroscopy ... angle of incidence is varied using a vibrating mirror which rotates 30 over an angle of 4° with a frequency of 44 Hz In each cycle, SPR curves were scanned on the forward and backward movement of...

Ngày tải lên: 03/10/2015, 20:32

... also the selective efficiency of the UOH of H and of the VOH or UCH modes of CH3OH Quantum yields for the C —> U photoconversion of the HI/H complex were estimated of 0.75 and 0.41 respectively ... band does not appear in the spectrum of either component alone The position of the band is often related to the ionization of the donor, the electron affinity of the acceptor and the charge separation ... Molecular Orbital (LUMO) of the acceptor and the exact strength of interaction depends on the difference in energy of the two concerned orbitals The concept of hard and soft acids and bases (HSAB...

Ngày tải lên: 05/07/2016, 10:40

... carried out using XLSTAT software (Addinsoft, France) 2.4.2 Quantitative descriptive data analysis Data from the Quantitative Descriptive Analysis was analysed by means of the ANOVA test using the ... lx) First of all, consumers were briefed on the concept of the methodology and the procedure The aim of this was to allow for development of the vocabulary to describe the four types of dry-cured ... 55.58% of the Table Demographic, socio-economic characteristics and frequency of dry-cured ham consumption of the participants (% of respondents, n = 109) Socio-demographic characteristic % of respondents...

Ngày tải lên: 03/04/2013, 21:07

... II melt of Form II Form I to Form II melt of Form II loss of water melt of Form I Form I to Form II melt of Form II loss of water melt of Form III melt of Form VI loss of water melt of amorphous ... loss of water melt of Form III? loss of water melt of Form III Form I to Form II melt of Form II loss of water melt of Form III Form I to Form II melt of Form II Form I to Form II melt of Form ... Form II melt of Form II melt of Form VI melt of Form VI Form I to Form II melt of Form II loss of water melt of Form VI loss of water melt of Form VI Table Summary of combined DSC/XRD data, suggested...

Ngày tải lên: 14/02/2014, 03:20

... media containing isoform I Both isoforms were also expressed intracellularly, as evidenced by the appearance of protein bands of the expected size upon SDS ⁄ PAGE analysis of the crude extracts ... recombinant isoforms Lane M, molecular mass standards production of the human recombinant isoforms might be enhanced by the presence of metal ions in the growth medium We found that addition of 200 ... the mean of three independent experiments and are presented as copies of the target variant per 109 copies of 18S RNA Quantitation of ACMSD variants in liver, kidney and brain An isoform-specific...

Ngày tải lên: 19/02/2014, 02:20

... determination of TNF-a in duplicate at two different dilutions The data are representative of three independent measurements LAL assay The LAL test is based on the property of lysates of amebocytes of the ... form a solid gel in the presence of minute amounts of endotoxins The comparison of LPS and MfGl-II in the LAL assay shows, as expected, activity of LPS in the range of down to 10 pgÆmL)1, whereas ... Endotoxin activity of the glycolipids was determined by a quantitative kinetic assay [27] based on the reactivity of Gram-negative endotoxin with Limulus amebocyte lysate (LAL) using test kits from...

Ngày tải lên: 21/02/2014, 00:20

... Brownian rotation of the fluorescent nucleotide Fig Binding of Ant-dATP to AC (d) and AC–CaM (m) The degree of binding of the ligand was calculated from the value of the amplitude of the long lifetime, ... residues of the catalytic site, favors a new activation mechanism Recently, the resolution of the 3D structure of the AC exotoxin of B anthracis and of its complex with CaM led to the proposal of a ... a direct exploitation of the structural results of the former to explain the mechanism of CaM activation of the latter Crystallization and resolution of the 3D structures of AC and AC–CaM, which...

Ngày tải lên: 07/03/2014, 15:20

... degree of substitution of the resin, 0.5 mmol of Na-Boc-Met per gram of resin was used for Na-Boc-Met coupling, yielding after acetylation (acetic anhydride/ dichloromethane, : 5), a substitution of ... the Teflon vessel of an HF apparatus and the peptide was cleaved from the resin by treatment with 1.5 mL of anisole, 0.25 mL of dimethyl sulfide, and 10 mL of anhydrous HF per gram of peptide-resin ... concentration of agonist A, in the presence of the antagonist B, which is equiactive to [A], the concentration of agonist in the absence of antagonist Values presented are the mean ± SE of at least...

Ngày tải lên: 08/03/2014, 02:21

... the sum of the individual components consequence of the use of D2O buffer This is the consequence of the H–D exchange that takes place when using D2O buffer, a range of that was quantified using ... structure of the catalytic domain of PKCf and the effect of its substrate, MgATP, and also to study the effect of thermal unfolding in the presence and the absence of MgATP We used 2D correlation spectroscopy ... solvent Comparison of the secondary structure of PKCf with those of other protein kinases of the AGC family (Table 2) offers other interesting data The secondary structure of PKCi, as deduced...

Ngày tải lên: 16/03/2014, 13:20

... electron microscopic image of the morphology of the SiNWs It was observed that the product exemplified high purity with a uniform diameter 20 nm and consisted of most of the SiNWs aligning on the ... shows the Raman scattering spectra of the SiNWs and crystal silicon A very sharp and Fig A typical SEM image of the morphology of aligned silicon nanowires of uniform diameter distribution on ... width at half maximum (FWHM) of 4:7 cmÀ1 was witnessed in the Raman spectrum of crystal Si This peak corresponds to the degenerate zone-center optical phonon mode of crystal Si All SiNW samples...

Ngày tải lên: 16/03/2014, 15:06

... generation of Ab peptides in pathological processes Study of the characteristics of the PS complex will facilitate elaboration of the molecular processes involved in the production of Ab Analysis of ... the low SDS concentration of 0.1% with 0.2% DDM, PS1 subcomplexes of 90 and 60 kDa were detected (Fig 3B) We also investigated the association of NCT with PS complex using embryonic mouse brain ... regions of APH-1a (short form) and APH1b, and to the loop four region of APH-1b (data not shown) Investigation of noncarbonate washed embryonic mouse brain PS complex revealed similar detection of...

Ngày tải lên: 16/03/2014, 16:20