quantitative assessment of neurite outgrowth in pc12 cells

... average length of neurites in PC12 cells treated with various NGF concentrations Table 3.5 Influence of DC EMF with intensity of 0.016 mT on percentage of neurite- bearing cells in PC12 cells treated ... 3.3 Influence of DC EMF with intensity of 0.19 mT on percentage of neurite- bearing cells in PC12 cells treated with various NGF concentrations Table 3.4 Influence of DC EMF with intensity of 0.19 ... List of Tables Table 1.1 Sources and intensity levels of electromagnetic fields in residences Table 3.1 Influence of DC EMF with intensity of 1.37 mT on percentage of neurite- bearing cells in PC12...

Ngày tải lên: 26/09/2015, 10:13

... recognizing imprinting in mice using the non-equivalence of monoallelic expression in reciprocal matings of inbred strains but yielded widely different estimates of amounts of imprinted genes in mouse ... what the extent of imprinting is in humans Currently, direct assessment of imprinting in human tissues has yielded approximately 80 genes with varying degrees of evidence for imprinting [23] and ... growth and imprinting, they have likely evolved more than once [7] The debate around theories of imprinting parallels the intense investigation of the mechanisms that maintain imprinting Monoallelic...

Ngày tải lên: 09/08/2014, 22:24

... required staining Figure shows that even without staining this imaging method can readily explore in detail the dissimilarities in the internalization of different AuNPs by different cell lines Specifically, ... EMT cells after co-culturing with 500 μM naked AuNPs for 48 h The size distribution in A) was obtained by analyzing individual cells such as that in B) Bars in B: 10 μm Chen et al Journal of ... Figure 8C and 8D (movie of different projection in Additional file 2) shows the results of specimen staining in revealing the detailed localization and shape of the nucleus and of the overall nanoparticle...

Ngày tải lên: 11/08/2014, 00:23

... players in biological systems to enable understanding in protein-protein interactions or protein-DNA interactions A number of techniques, including protein microarrays, pull down assays and chromatin ... dynamics of membrane, cytosolic and nuclear proteins in living cells and organisms In this work I provide guidelines on the building and characterization of microscope setups, on the suitability of ... imaging method can provide spatio-temporal dynamics and binding maps of proteins localized in membrane, cytosol and nucleus This particular study combines fast SPIM imaging (single plane illumination...

Ngày tải lên: 09/09/2015, 11:19

... function are involved in a variety of important biological responses, the significance of ERK signaling in terms of regulating miRNA expression is of great interest To study the role of the ERK1 ... pri-miRNA [21] Following NGF stimulation, the expression level of Fig NGF induces expression of the microRNAs miR-221 and 222 in PC12 cells (A) NGF-induced differentiation of PC12 cells (B) Schematic ... involved in NGF-dependent neuronal survival [17–19], as a potential target of miR-221 and 222 Results NGF stimulation induces miR-221/222 in PC12 cells Treatment of PC12 cells with NGF for 48 h induced...

Ngày tải lên: 16/03/2014, 01:20

... limiting component of TGF-b1 signaling in PC12 cells Smad2 phosphorylation was analysed in parental PC12 cells and in stable PC12 cells expressing TbRII-wt under the control of a doxycyclineinducible ... transfection of PC12 cells with TbRII but not TbRI constructs leads to increased responsiveness to TGF-b1 (Fig 2B), again indicating that TbRII is the limiting component of TGF-b1 signaling in PC12 cells ... activation of the Smad pathway independently of the TGF-b ligand Fig PC12 cells express low levels of endogenous TbRII (A) C-terminal phosphorylation of Smad2 was investigated in either parental PC12 cells...

Ngày tải lên: 16/03/2014, 16:20

... subsequent determination of optical density confirmed the presence of actin in CAD cells (Fig 1B, lanes and 2) in an actin ⁄ tubulin proportion slightly higher than that determined in mouse brain [0.16 ... Mouse brain tissue was used as a positive control The reduced amount of Glu-tubulin in CAD cells was not a result of a lack of (or inhibition of) the putative detyrosinating enzyme (tubulin carboxypeptidase) ... (2002) Incorporation of 3-nitrotyrosine into the C-terminus of alpha-tubulin is reversible and not detrimental to dividing cells Eur J Biochem 269, 5037–5045 Neurite formation in CAD cells 59...

Ngày tải lên: 23/03/2014, 05:22

... quantitation of microgram quantities of protein utilizing principle of protein-dye binding Anal Biochem 72, 248 254 34 Tang D, Shafer MM, Vang K, Karner DA & Armstrong DE (2003) Determination of dissolved ... data (black line, A,B,C), tting a singleenzyme model (blue line, A), tting a twoenzyme model (red line, B) and tting a single-enzyme model with competitive product inhibition (yellow line, C) The ... metabolites in Leishmania infantum promastigotes (A) Analysis of 2-oxoaldehydes, showing the presence of MG as 2-methylquinoxaline and the internal standard (IS, lM 2,3-dimethylquinoxaline) Other...

Ngày tải lên: 23/03/2014, 13:20

... dUTP in the reaction mixture in place of dTTP and in the case of some manufacturers to include UNG as a standard reagent in kits The success of this approach for elimination of contaminating amplicon ... theoretically eliminated as a source of template that would cause false positives in PCR or RT-PCR UNG degrades contaminating uracil-containing DNA while leaving the natural, thymine-containing DNA intact ... dependent increase in CT for RNA amplification was also noted, with incubation temperatures of 25°C and 30°C resulting in smaller increases in CT values for RNA detection than incubation at 35°C Incubation...

Ngày tải lên: 19/06/2014, 08:20

... dUTP in the reaction mixture in place of dTTP and in the case of some manufacturers to include UNG as a standard reagent in kits The success of this approach for elimination of contaminating amplicon ... theoretically eliminated as a source of template that would cause false positives in PCR or RT-PCR UNG degrades contaminating uracil-containing DNA while leaving the natural, thymine-containing DNA intact ... dependent increase in CT for RNA amplification was also noted, with incubation temperatures of 25°C and 30°C resulting in smaller increases in CT values for RNA detection than incubation at 35°C Incubation...

Ngày tải lên: 20/06/2014, 04:20

... was maintained in the left eye The adenoma gradually progressed, eventually requiring SRT using 25 Gy in equal fractions (5 Gy × times in consecutive days) for a minimum coverage of 90% of the ... outcomes, not only in contouring and the setting of dose constraints, but also for planned dose distributions especially in a multi-clinician study We should keep in mind the risk of such new techniques ... second with meningioma Variability in contouring, planning target volumes, prescribed doses, and doses to OARs was assessed In addition, we examined the influence of different contouring especially...

Ngày tải lên: 09/08/2014, 09:20

... Since PC12 cells are commonly used as a dopaminergic model, in order to investigate involvement of NCS-1 in dopaminergic intra- Page of cellular signaling pathways, we established PC12 cell line ... acting as a key downstream effector in transducing dopamine signaling, integrating the signaling of different neurotransmitters and neuromodulators [18] Desensitization and internalization of ... Abnormalities in the dopamine system in schizophrenia may lie in altered levels of dopamine receptor-interacting proteins Biol Psychiatry 2004, 56:427-40 Stoof JC, Kebabian JW: Opposing roles for...

Ngày tải lên: 11/08/2014, 07:21

... people in Chechnya and 283 people in Ingushetia were interviewed None of those approached for interviewing refused and no interviews were interrupted (i.e 100% completion) The vast majority of interviewees ... confronted with traumatic events in several ways, including exposure to an event (being in the area but not witnessing or self-experiencing an event), witnessing of an event (seeing the event happen) and ... Origin Chechnya Other Reason for not returning to place of origin Lack of shelter Insecurity Other Reasons for returning to Chechnen TACs Living circumstances in spontaneous settlements Ingushetia...

Ngày tải lên: 13/08/2014, 13:21

... documented species in the Philippines and in Singapore .45 Table 2.3 Elevation significantly predicts height in rainforest canopy of frogs 48 Table 2.4 Height in rainforest canopy ... partitioning of species in rainforest driven by a steep microclimatic gradient may provide some level of compensation against changing microclimates, by allowing species to shift vertically within ... frog usage in the canopy Lastly, using a dataset for all frogs of the Philippines, I explored and predicted how arboreality in frog assemblages was likely to increase with increasing elevation...

Ngày tải lên: 10/09/2015, 09:11

... localization of claudin-1 Nuclear claudin-1 does not increase melanoma cell motility We have previously shown that increasing the levels of CLDN-1 increases the invasion of melanoma cells (4) To determine ... involved in claudin-1 expression and localization 99 Indeed, a study shows that increasing β-catenin increases the expression of claudin-1 in colorectal cancer cells, and that claudin-1 expression ... isoforms using bryostatin instead increased the expression of claudin-1 in the tight junction complex, and increased tight junction barrier integrity (9) In melanoma, we have shown that claudin-1 expression...

Ngày tải lên: 03/11/2012, 11:17

... purposes, including drinking water supply, washing, bathing, transportation, fishing, and, in recent years, gold mining Although, importantly, gold mining contributes 19.4% to 29.1% of the annual ... Control of Drinking Water (Appendix I: Drinking Water Quality Conditions) Health Ministry of Indonesia (in Indonesian) Regine, MB., Gilles, D., Yannick, D., Alain, B 2006 Mercury distribution in fish ... Sampling Points Main river Tributary Bar columns indicated averages of mercury concentrations; Lines indicated minimum and maximum of mercury concentrations Figure Total mercury concentrations in...

Ngày tải lên: 05/09/2013, 09:38

... and eosin staining We examined the expression of these proteins in a cancer-free layer of squamous epithelial cells, carcinoma in situ (CIS) and invasive cancer, all of which were observed in the ... levels in cancer cells derived from uterine cervix and in HeLa cells The expression of Mcm genes in growth-arrested cells is induced by addition of serum [43] There are several E2F binding sites in ... more abundant in HeLa cells than in WI-38 cells Synthesis of Mcm4, and proteins was accelerated in HeLa cells compared to WI-38 cells, but the turnover rate of the Mcm4 protein within 22 h did...

Ngày tải lên: 20/02/2014, 23:20

... sedimenting in the first third of the gradient and attributable to short chains originating from newly initiated replicons In the course of further 25 of reoxygenated growth, the incorporation of [3H]Thd ... end of the respective incubation conditions, in case of hypoxic labeling by plunging a spatula carrying the appropriate quantity in dried form into the cell culture medium To stop incubations cells ... (Axioskop) using the appropriate filter combinations Immunofluorescence staining of chromatin-bound PCNA and of replicating DNA Cells grown on coverslips were labeled by adding 15 lM 5¢-bromodeoxyuridine...

Ngày tải lên: 21/02/2014, 00:20

Ngày tải lên: 07/03/2014, 02:20

... the income uncertainty, and the incompleteness of the insurance market, households’ engage in precautionary savings to hedge away the risk of attaining “catastrophic” levels of income They accomplish ... for precautionary savings against low realizations of income shocks but it might introduce incentives to save against high realizations of income shocks if the degree of indexation is such that ... the corresponding value is −61.49 in the nonindexed case (since, the endowment is normalized to 1, those nominal values of debt limit are in units of GDP) In other words, in the full-indexation...

Ngày tải lên: 15/03/2014, 07:20