quantitative analysis and validation of method using hptlc

... chromatography (HPTLC) with spectrometric methods It is because of the robustness and simplicity of use of HPTLC and the need for detection techniques that provide identification and determination of sample ... implementation, optimization, validation, automation, and qualitative and quantitative analysis; applications of HPTLC separation with special reference to phytochemical analysis, biomedical analysis, herbal ... static, off-line detection of zones using a great variety of complementary post-chromatographic universal and selective detection methods that are often applied sequentially, and storage of the...

Ngày tải lên: 06/10/2016, 09:26

... traditionally employed method (i.e construction of an EST library, storage and sequencing of each clone using Sanger sequencing technology), using high-throughput approaches allows several thousand ESTs to ... when regions of high DNA sequence homology tandem repeats and tandemly duplicated genes are present Gene sequences of interest were identified successfully using BAC sequencing Using lip transient ... filters were 458 nm/band-pass 465-510 nm for CFP, 488 nm/bandpass 500-530 nm for GFP, 514 nm/ bandpass 525-555 nm for YFP, 561 nm/bandpass 575-630 nm for RFP and mCherry, and 488 nm/band-pass 650-710...

Ngày tải lên: 11/08/2014, 11:21

... Materials and methods Plasmid construction In general, PCR amplifications were performed with VentDNA-polymerase (New England Biolabs) The entire sequences of the dfp and coaB coding regions of the ... described [5] Purification and characterization of Dfp and CoaB proteins Growth of strains E coli M15 (pREP4, pQE8/pQE12) cells were grown in the presence of ampicillin (100 lgÆmL)1) and kanamycin (25 ... and 450 nm Molecular mass information was obtained as described [6], determining the elution volumes of standard proteins and the void volume of the column The Superdex 200 PC 3.2/30 column and...

Ngày tải lên: 19/02/2014, 12:20

... computer modelling of the MUC1 SEA domain (Fig 3) The model has the same a ⁄ b sandwich fold as the template SEA domain of murine MUC16 [12] and consists of three a-helices and six b-strands This model ... Ribbon representation of the modelled SEA domain of human MUC1 The model consists of three a-helices and six b-strands forming an a ⁄ b sandwich fold The FRPG ⁄ SVVV residues of the MUC1 cleavage ... MUC3SEA and MUC1FDTR ⁄ MUC12SEA glycoproteins showed a major M2-reactive and CT-2 reactive noncleaved glycoform of about 105–120 kDa (Fig 4A,C, MUC3SEA and MUC12SEA) and a minor population of a...

Ngày tải lên: 19/02/2014, 18:20

... logistical and methodological – and it is important that the Council of Europe and other organisations continue to learn from, and improve, their work in this field How the bodies who ask children and ... appetite for information and respect for their rights is clear To some extent, they are also aware of the unsatisfactory nature of delay and of cost and that the impact of these practical barriers ... could make it more child-friendly, the attitudes and behaviours of health care professionals and issues affecting communication and children’s use of health care services Accordingly, their report,...

Ngày tải lên: 17/03/2014, 17:20

... and C2-regions of p300, and to the N- and C2-regions of CBP Thus, the bulk of the interaction between NF-jB and p300/CBP is mediated by the p65 subunit through the N- and C-terminal regions of ... amount of transcription factors used in (A), with or without lg of IRF-1 or of an IRF3/7 mix and 0.75 lg of CBP; 4X, 2X and 1X correspond to decrease of the amount used in 8X by factors of 2, and ... ATF-2 and c-Jun can bind to p300 and CBP through multiple domains, with a preference of c-Jun for the N- and C-terminal regions and of ATF-2 for the central region of the coactivators Binding of...

Ngày tải lên: 23/03/2014, 13:20

... activity of this enzyme [10] Thus, Sec14p and its homologues appear to form a network of proteins involved in the regulation of phospholipid metabolism Materials and methods Strains and culture ... 2003 Ó FEBS 2003 nucleotides upstream of the STOP codon of the ORF of interest and on the 3¢ end, 65–70 nucleotides of the genomic region downstream of the ORF of interest Transformants were recovered ... localized by subcellular fractionation using standard techniques of organelle isolation [24] and Western blot analysis (Fig 5) EGFP hybrids of SEC14, SFH2 and SFH5, which were localized mainly...

Ngày tải lên: 23/03/2014, 18:20

... cancer and are an exciting class of compounds for potential treatment of these malignancies Methods Sigma receptor ligands Sigma-2 specific ligands SV119, SV95, and fluorescent labeled sigma-2 ligand, ... split and preincubated for more than 24 hours (Panc-02) and 48 hours (CFPAC-1, AsPC-1, and Panc-1) to maintain their growth conditions SV119 and SW120 were dissolved in DMSO, and gemcitabine and ... recently shown that selected sigma-2 ligands are capable of inducing apoptosis in a multitude of human and murine cancer cells lines and in animal models of cancer including pancreas cancer [12,13,15]...

Ngày tải lên: 18/06/2014, 15:20

... diagnosis of MDD and BD was established in consensus meetings of two psychiatrists (JS and HB) using all available information from interviews and clinical records [30] Brains with lifetime reports of ... exact thickness of each section was determined by focusing on the upper and lower surfaces of the section and subtracting the z-axis coordinate of the lower surface from that of the upper surface ... Illustrations of QUIN-immunoreactive cells from the left sACC of a depressed suicidal patient and a control case and the locations of the analyzed regions of interest (sACC, aMCC and pACC) Depressed...

Ngày tải lên: 19/06/2014, 22:20

... the dielectric constant of the reaction mixture and to repress the ionization of cresolphthalein complexone [38] + Material and methods Subjects and methods Measurement of potassium The study ... calculated using a typically treated standard solution of K + chloride in Bovine albumin equivalent to mol/L Page of Table Caspase3, IL6, TNFa, Ca2+, Mg2+, Na+ and K+ concentrations and Ca2+/Mg2+ and ... mechanism relating Ab and Ca2+- induced apoptosis in brain cells of Saudi autistics Table ROC analysis of Ca2+/Mg2+ and Na+/K+ ratios and Caspase3, IL6, TNFa, Ca2+, Mg2+, Na +and K+ in autistic groups...

Ngày tải lên: 19/06/2014, 22:20

... NAG-2-1286, NCC2-1361 and the Department of Veterans Affairs Medical Research Service in support of MHF and this project We thank Sandra Spurlock for the data plate reading and data analysis of Table We ... expression of mineralizing genes Hughes-Fulford and Li Journal of Orthopaedic Surgery and Research 2011, 6:8 http://www.josr-online.com/content/6/1/8 Figure qRTPCR analysis of gene induction of proliferation ... hour of each gene) Relative mineralization of FGF-2 and BMP-2 treated cells As seen in Figure and Table 3, BMP-2 treatment enhances mineralization of the cells as shown by uptake and presence of...

Ngày tải lên: 20/06/2014, 04:20

... NAG-2-1286, NCC2-1361 and the Department of Veterans Affairs Medical Research Service in support of MHF and this project We thank Sandra Spurlock for the data plate reading and data analysis of Table We ... expression of mineralizing genes Hughes-Fulford and Li Journal of Orthopaedic Surgery and Research 2011, 6:8 http://www.josr-online.com/content/6/1/8 Figure qRTPCR analysis of gene induction of proliferation ... hour of each gene) Relative mineralization of FGF-2 and BMP-2 treated cells As seen in Figure and Table 3, BMP-2 treatment enhances mineralization of the cells as shown by uptake and presence of...

Ngày tải lên: 20/06/2014, 07:20

... + μb , (1) σPAN where μPAN and μb are the mean of the PAN and MS image band b, respectively, and s PAN and s b are the standard deviation of the PAN and MS image band b, respectively This technique ... MS bands and the resampled LR MS bands The spatial distortion index defined in [118] is based on differences between the UIQI of each band of the fused image and the PAN image and the UIQI of ... weighted mean of MS image These weights depend on the spectral response of the MS bands and high-resolution PAN (HR PAN) image and on the optical transmittance of the PAN band The second method simulates...

Ngày tải lên: 20/06/2014, 22:20

... constant of C mineralization rate, C0k – potential rate of initial C mineralization 342 – NO3 -N was measured by the method of ultravio+ let spectrophotometry and NH4 -N by the indophenol blue method ... mineralization rates decreased and finally reached a steady state for all treatments The significant values of ANOVA analysis (univariate analysis of GLM program) showed that on all days of incubation both ... water content of 40%, while the C0 difference of soils at the elevation of 1,600 m and 1,800 m was not significant However, this trend was not suitable for C0 at water contents of 20% and 60% At...

Ngày tải lên: 07/08/2014, 10:21

... theoretical understanding of the relation between measurable parameters of the heat field and sap flow and for methodical aspects of sap flow techniques MATERIALS AND METHODS 2.1 Site and sample trees ... profiles of water flow in stems of spruce and oak and response of spruce tree to severing, Tree Physiol 10 (1992) 367–380 [7] Granier A., A new method to measure the raw sap flux in the trunk of ... IR images of heat field and sap flow of lime and extremely low flows (those approaching zero, such as resaturating flows at night) Fast responding and high sensitivity of the HFD -method to any...

Ngày tải lên: 08/08/2014, 01:22

... accumulation of organic compounds such as amino acids or soluble carbohydrates (Turner and Jones, 1980; Morgan, 1984) We studied the effect of a moderate soil drought on the rate of CO assimilation and ... drought on the rate of CO assimilation and the amount of soluble and insoluble carbohydrates in leaves of 4-year-old saplings of Q petraea The aim of these experiments was to assess whether the decline ... change in the partitioning of photosynthates and whether this change reflected an increased requirement of soluble carbohydrates for osmotic adjustment MATERIALS AND METHODS Plant material Four-year-old...

Ngày tải lên: 08/08/2014, 18:21

... basis of genome size relative to a standard plant of known ploidy MATERIALS AND METHODS The plant material was leaves of in vitro cloned plantlets of Q petraea (Matt) Liebl, Q pubescens Willd and ... plants This type of data can be useful in planning molecular procedures for DNA polymorphism analysis or the study of genetic architecture of genomes by RFLP and PFGE (choice of rarely cleaving ... petraea 2C 1.6 pg (Band, 1984 in Bennett and Smith, 1991), 1.8 pg (Greilhuber, 1988) or 1.58 pg (Ohri and Ahuja, 1990), Q robur 2C= 1.59 pg (Ohri and Ahuja, 1990), 1.61 pg (Ohri and Ahuja, Q rubra...

Ngày tải lên: 08/08/2014, 18:21

... plants of Q robur, Q petraea and Q cerris, purchased in root trainers, were potted for use in 1992 and 1993 Two groups of 10 plants each, chosen from of the species, were droughted for periods of ... measurements using the method of Sperry et al (1988a) Each leaf was cut from its stem under degassed water and its petiole excised from the base of the leaf lamina before cutting to a length of 20 mm ... withstand such stresses potential, stomatal conductance and cavitation on a sapling of Thuja occidentalis L Plant Cell Environ 7, 615-618 Grieg BJW (1992) Occurrence of decline and dieback of oak...

Ngày tải lên: 08/08/2014, 19:21

... at 35 and 90 cm deep at the 40-year stand, the 40-year boggy stand and the 140-year stand, and with manually recorded tensiometers at 20 cm deep at the 40-year NE stand, the 70-year stand and the ... crown of five harvested trees at the 70-year and at the 110-year stands and at the end of October 1994 from five trees of the 40-year, the 40-year boggy and the 40-year NE stand Nutrient content of ... stand Predawn water potentials of small twigs of the trees at the 140-year, 40year, 40-year boggy and 40-year NE stand were measured every weeks from the end of June to the middle of August, using...

Ngày tải lên: 09/08/2014, 04:20

... of Economics and Director of the Centre for Study of Global Governance at the London School of Economics Sheila Dow is Professor in the Department of Economics, University of Stirling, Scotland ... demand and supply diagram in r, Y space 15.2 Alternative presentation of aggregate demand and supply in r, Y space 15.3 Adjustment to a lower real rate of interest 15.4 Effect of effective demand ... Ontario and a Fellow-in-Residence of the C.D Howe Institute, Cananda John Nevile is Emeritus Professor and Visiting Professor at the University of New South Wales, Australia Alain Parguez is Professor...

Ngày tải lên: 09/08/2014, 19:22