quantification of lacz expressing vectors by contrast phase microscopy

... compositions are 48.77 at% of O, 41.87 at% of Si, 8.66 at% of V, 0.74 at% of Zn, and no Cl, for the catalyst tip in Fig 2(b), and 60.28 at% of O, 39.40 at% of Si, 0.32 at% of V, and no Zn or Cl, ... mixture of ZnCl2, and VO2 on silicon substrate by controlling the conditions of the vapor -phase transport Ultra-long cotton-like nanowires with average diameters of $50 nm show both strong PL of red ... well to avoid excessive etching of substrate It is worth mentioning that, although both ZnOx and SiClx are in vapor phase, SiClx is an intermediate phase of a series of chemical reactions, and it...

Ngày tải lên: 16/03/2014, 15:19

... TABLE OF CONTENTS TABLE OF CONTENTS Page ACKNOWLEDGEMENTS i TABLE OF CONTENTS .ii SUMMARY vi LIST OF TABLES ix LIST OF FIGURES xi LIST OF ... Drying of spheroids .35 2.7 Characterization of spheroids .35 2.7.1 Size analysis by sieving .35 2.7.2 Sizing by image analysis 36 2.7.3 Roundness quantification by ... cores The breakdown of the extrudates is followed by extensive layering of the fines Extensive layering of fines would occur during spheronization In contrast, spheroids produced by rotary processing...

Ngày tải lên: 11/09/2015, 16:05

... height image of cross-sectional nacre, (c) phase image of cross-sectional nacre, (d) height image of nacre surface, (e) height image of cross-sectional calcite, (f) phase image of cross-sectional ... development of Piezoresponse Force Microscopy (PFM), one of the functional modes of Scanning Probe Microscopy (SPM) technique, the studies of the piezoelectric and ferroelectric behaviors of biomaterials ... behaviors of seashell by piezoresponse force microscopy, Journal of Applied Physics 113 (2013), 187202 T Li, L Chen, and K Zeng, In situ studies of nanoscale electromechanical behavior of nacre...

Ngày tải lên: 10/09/2015, 09:23

... neutralized by anti-G antibody but not by high amounts of anti-CD4 antibody Env- cells were only infected by HIV-1-Neo(G) and not by HIV-1Neo(CD4) particles These results confirm the specificity of the ... stable targeted transduction of HIV-1 Env expressing cells by HIV(CD4) particles was not detected by us previously [6,7] A lack of CXCR4 insertion can now be ruled out by the data presented above ... simultaneously released by the cell and cannot be separated by this gradient [37] Solubilization of the viral membrane by 0.4% Triton X-100 shifted the p24 antigen to the top of the gradient (Figure...

Ngày tải lên: 13/08/2014, 09:21

... respectively Quantification of microcystin-degrading bacteria by real-time PCR The standard curve was generated from serial 10-fold dilutions of DNA carrying the mlrA of strain C-1 The quantification of ... reference** Rapala et al., 2005 Isolated by our research group Provided by Dr Fujimoto from Tokyo University of Agriculture ** Biofilm samples Samples of biofilm (10 g) were taken from a biological ... reactions required µL of extracted DNA, 10 pmol of each primer, 30 pmol of TaqMan probe, 25 µL of TaqMan Gene Expression Master Mix (Applied Biosystems Co USA), made up to a final volume of 50 µL using...

Ngày tải lên: 05/09/2013, 10:15

... ES On the contrary, expressing appreciation and offering reward are both preferred by ES Expressing gratitude by ES usually appears in the form of Thanking + Complimenting + Expressing appreciation ... Complimenting + Offering return Thanking + Expressing appreciation + Expressing indebtedness Expressing gratitude by VL usually appears in the form of Thanking + Complimenting + Expressing appreciation ... Expressions of gratitude by ES appear in the formula of Thanking or Thanking + Expressing appreciation And the act for expressing gratitude by VL can be formularized in the formula of Thanking...

Ngày tải lên: 29/01/2014, 14:36

... obtained by concentration of 293T-conditioned media through ultracentrifugation at 50 000 g for 140 Quantification of viral content was performed by immunocapture of HIV-1 p24 antigen by using ... Transduction efficiency was evaluated by detection of fluorescence after infection of MPS I cells with 20 ng of lentiviral-GFP; efficiency was calculated as number of Table a-L-Iduronidase activity ... first reported an expression of high levels of human iduronidase after retroviral transduction of MPS I fibroblasts The transduction of hematopoietic stem cells was studied by Fairbain et al [13],...

Ngày tải lên: 24/03/2014, 04:21

... involvement of IL-6 in Immune responses with S cerevisiae expressing rApxIA or rApxIIA 391 the production of IgA and the induction of TNF-α by IgA [23] Moreover, the dual capacities of secreted ... sections, assessed by routine staining with hematoxylin and eosin, and examined by light microscopy The inflammatory response was evaluated by examining the lung tissue for the presence of typical inflammatory ... probability values of p ＜ 0.05 were obtained Results Immunogenicity of yeast expressing ApxIA antigen To initially confirm the immunogenicity of the yeast-derived ApxIA antigen, the production of ApxIA-specific...

Ngày tải lên: 07/08/2014, 20:23

... cells receptor of monocyte expression of type and rheumatoid arthritis Induction(IL-10R1/2) by culture supernatants of type interleukin-10 receptor (IL-10R1/2) by culture supernatants of rheumatoid ... analysed by flow cytometric analysis (c) Induction of monocyte expression of TNFR1 and TNFR2 by IL-10 and/or M-CSF The cytokine-mediated TNFR1/2 induction was expressed by the ratio of the mean ... measured by real-time PCR As shown in Figure 4, expression of both TNFR receptors was augmented at the transcriptional level by IL-10 RA monocytes were more responsive to IL-10 in terms of Page of...

Ngày tải lên: 09/08/2014, 08:22

... of human serum (Figure 6) But the relaxation time is by a factor of 1.4 smaller than that of the serum sample This difference is related to the viscosity of serum of about mPa s being twice of ... We diluted the stock suspension of biotin-agarose by a factor of with PBS The MNP*SAV were diluted with PBS by factors of and 10; MNP*AB were diluted by factors of 10 and 100 For the MARIA-measurement ... the signal of the transducers changes as the result of the binding of the detection molecule, i.e the amount of bound detection molecules can be measured in the presence of unbound ones by separating...

Ngày tải lên: 11/08/2014, 00:22

... titer of rAAV is relevant for gene therapy only if the rAAV is capable of serving as a vector for a therapeutic agent In the present study, we tested the function of these rAAV vectors by incorporating ... the utility of rAAV vectors for the delivery of therapeutic transgenes to target cells Further studies will be required to optimize the value of rAAV through this and other mechanisms of rAAV synthesis ... once with ml of DMEM with 2% FBS and removed again The cells were transduced by adding 125 μl of rAAV-IGF-I and 125 μl of DMEM with 2% FBS to each well After hour incubation, 750 μl of growth medium...

Ngày tải lên: 12/08/2014, 04:21

... ANALYSIS OF INTEGRATION SITES OF TRANSGENIC SHEEP GENERATED BY LENTIVIRAL VECTORS USING NEXT-GENERATION SEQUENCING TECHNOLOGY A Thesis Submitted to the Faculty of Purdue University by Yu-Hsiang ... 2013 Analysis of Integration Sites of Transgenic Sheep Generated by Lentiviral Vectors Using Next-Generation Sequencing Technology Major Professor: Anna Malkova The development of new methods ... follows: ul of 10X PCR buffer (Qiagen), ul of 10 mM dNTP, 0.5 ul of 50 uM LTR II-bio primer (IDT), 0.5 ul of 50 uM LC1 primer (IDT), ul of Taq Polymerase (5 units/ul, Qiagen), ul of DNA from...

Ngày tải lên: 24/08/2014, 13:51

Ngày tải lên: 21/09/2014, 14:34

... range of 3.0–8.0 by using model solutions containing lg of cadmium(II) and 20 lg of lead(II) The results are depicted in Fig Quantitative recovery values were obtained at the pH range of 6.0–7.0 ... All other works were done at pH 6.5 by using phosphate buffer 3.2 Amounts of ligand The influences of amounts of 1-phenyl-1H-tetrazole-5-thiol on the retentions of Cd(II) and Pb(II) on Diaion SP-207 ... effects of pH of the aqueous Table Because the flow rates of sample solution and eluent solutions are two important parameters for the quantitative retention of analytes on the solid phase extraction...

Ngày tải lên: 02/09/2015, 13:45

... different phases (1, 2, 3, 4a, 4b, and 4c) by adopting the terminology of Shirokova (1977) In contrast to Shirokova (1977), phase 4a in the present study was characterized by a distinct CNR instead of ... these two phases were combined into phase 4bc” during estimation of oocyte numbers (see below) The range in oocyte diameter (OD) for each phase was tabled and contrasted with the data of Shirokova ... following key components: Assessment of the fresh V ovary by use of Scherle’s (1970) method Prediction of the average fresh volume of oocytes in different PVO phases (4a, 4b, and 4c) and in subsequent...

Ngày tải lên: 04/09/2015, 12:47

... sequence of PMT 64 2.2 The map of the vectors 64 PTB 65 3.1 The PTB sequence 65 3.2 Map of the vector of the plasmid 66 Map of mRFP-EGFR-EGFP ... 121 7.2.1 The heterogeneity of EGFR diffusion revealed by ITIR-FCS diffusion law 121 7.2.2 The effect of cholesterol depletion by mβCD on the mobility of EGFR 122 7.2.3 Cytoskeletal effects ... coefficients of Dil analogues 109 Table Summary of diffusion coefficients 134 Table Summary of intercepts 135 x List of Figures Figure 1 The Crystal Structure of the 2:2...

Ngày tải lên: 10/09/2015, 09:25

... Figure 1.2 Evolution of number of transistors packed into each of Intel’s new generation of microprocessors, describing Moore’s law [2] .3 Figure 1.3 Cost of fabrication of transistors, DRAM ... leakage profile of the various diodes fabricated The change in the slope of the leakage profile at a bias larger than 2V is due to the onset of GIDL current 83 Figure 5.8 XTEM images of the ... half that of the influenza virus or about 2000 times smaller than a strain of human hair For a sense of the physical size of the transistor, Fig 1.7 shows a micrograph image of a strand of hair...

Ngày tải lên: 12/09/2015, 09:28

... support, love and the happiest moments she brings to my life.      II    Table of Contents    Acknowledgements       II  Table of Contents      III  Summary      VI  List of Tables    VIII   List of Figures      IX  List of Symbols and acronyms   ... A correlation between two variables a and b describes the dependence of these two  variables. In practical terms this means that if we know the value of a or b we can  make  some  prediction  of the  value  of b  or  a,  respectively.  The  concept  of ... photophysical  dynamics  of fluorophores,  diffusion  coefficients of DNAs and proteins, and dissociation constants of interacting particles.  In  this  work,  we  extended  the  application  of FCS  and ...

Ngày tải lên: 14/09/2015, 08:41

Ngày tải lên: 04/10/2012, 10:02