quantification of isoprostanes as an index of oxidant stress

... blank)/A control] × 100, where A control was the absorbance of the control group in the hydroxyl radicals generation system, A sample was the absorbance of the test group and A sample blank was ... ability of the TMP-A to scavenge hydrogen peroxide was determined according to the method of Smirnoff and Cumbes [17] The percentage of scavenging of hydrogen radicals was calculated as follows: ... the absorbance of the samples only Vc was used as a positive control in the study 2.9 Determination of superoxide anion scavenging activity Superoxide anion scavenging activity was measured according...

Ngày tải lên: 28/06/2014, 11:20

... was also determined and compared with those of the synthesised compounds (Fig 3) The antioxidant activity of BHA and AA was still lower than that of the compounds (a) and (d) In general, the antioxidant ... Frankel, E., German, B & Kanner, J (1993) Possible mechanisms for the protective role of antioxidants in wine and plant foods Food Technol., 47, 85–89 Behl, C & Moosmann, B (2002) Antioxidant neuroprotection ... 5Hdibenz[b,f]azepine and some of its derivatives can be used as a source of synthetic antioxidants, which could contribute to health benefits Studies on the antioxidant activities of newly synthesised...

Ngày tải lên: 07/08/2014, 14:20

... estimate of lipophilicity widely acknowledged as an important factor in transport across membranes A common measure of the degree of BBB penetration is the ratio of the steady-state concentrations of ... important to the differentiation of the biological profile between compounds PBIA and TPBIA The association of increasing molecular weight with increasing antagonistic power is well known An antagonist ... presence of the tosyl group might shift the agonistic activity to that of an antagonist It is documented that increasing the van der Waals molecular volume of an agonist makes it an antagonist...

Ngày tải lên: 08/08/2014, 20:23

... anti-rabbit IgG as the secondary antibody DAB (diamminobenzidine) was used as substrate [32] The intensity of bands was analyzed by image analyzer2254 The equal loading of sample in each lane was confirmed ... by Ayurvedic physicians of Indian system of medicine [6], but neither its mechanism of action nor the active principle for its antioxidant and anti-inflammatory property has been explored so ... 37 Chaurasia S, Tripathi P, Tripathi YB: Antioxidant and anti-inflammatory property of Sandhika: a compound herbal drug Indian J Exp Biol 1995, 33(6):428-32 Pandey and Tripathi Journal of Inflammation...

Ngày tải lên: 11/08/2014, 03:20

... absorbance of the control at t = and AA(t) is the absorbance of the antioxidant at t = h Determination of Ferric Reducing Antioxidant Power (FRAP Assay) The total antioxidant potential of a sample ... sample was determined using the ferric reducing ability of plasma (FRAP) assay of Benzie and Strain30 as a measure of »antioxidant power« The FRAP assay measures the change in absorbance at 593 ... without addition of antioxidant was heated at 110 °C and an airflow of 20 L/h was constantly blown into the mixture The antioxidant activity index (AAI) was calculated from the measured induction...

Ngày tải lên: 16/08/2014, 22:31

... would like to thank the supervisor of this project, Assistant Professor Huang Dejian and co-supervisor Associate Professor Benny Kwong-Huat Tan for providing the guidance, support and courage to ... AGE-products - Advanced-Glycation End products ALT - Alanin Transaminase AMPK - Adenosine Monophosphate-activated protein kinase ANOVA - Analaysis of Variance AST - Aspartate Transaminase ATP - Adenosine ... B.; 2000 Oxidative stress markers in human disease: applications to diabetes and to evaluation of the effects of antioxidants In Packer et al., (ed) Antioxidants in Diabetes Management Marcel Dekker,...

Ngày tải lên: 12/09/2015, 10:58

... identified Antihypertensive, antioxidant, antiinflammatory, antinociceptive [40] Antimicrobial, analgesic, inflammation, antioxidant [44] Antimicrobial [48,49], gastroprotective [50], antioxidant [51,52], ... 788 21.2 Medicinal Plant Research in Africa Sources of Antioxidants An array of intracellular and extracellular antioxidant mechanisms are essential to scavenge any oxidant “reactive intermediates,” ... is an inverse relationship between the dietary intake of antioxidant-rich foods and the incidence of human disease [19] However, synthetic antioxidants, such as butylated hydroxytoluene (BHT) and...

Ngày tải lên: 12/01/2016, 18:24

... Karabulut I, Balkanci ZD, Pehlivanoglu B, Erdem A, Fadillioglu E Effect of toluene on erythrocytes membrane stability under in vivo and in vitro conditions with assessment of oxidant/ antioxidant status ... oxidative modification of erythrocytes [10] Antioxidants can help in preservation of an adequate antioxidant status; therefore, they preserve the normal physiological function of living cells by ... volume of ml After boiling for h, the mixture was cooled, and ml of n-butanol and pyridine (15:1) solution was added to it This mixture was then centrifuged at 5000 rpm for 15 and the absorbance was...

Ngày tải lên: 25/10/2012, 11:40

... absence of antioxidants as a control of antioxidants as a control (slope = 0) The slopes, krrs = 0.79 (for C60), 1.33 (for C60O), and 1.93 (for C60On), represent the efficiency of the antioxidants and ... fullerene}/kobs of control) of PVP/C60 and C60O were 50% and 68% in 10 lM for antioxidant, and 73% and 81% in 30 lM, respectively Here, it is more convenient to define the absolute antioxidant activity of ... ¼ ð1Þ It was found that the b-carotene bleaching was significantly suppressed by the increasing amount of antioxidants, although C60O was more effective than C60 in all tested ranges of concentration...

Ngày tải lên: 22/06/2014, 01:20

Ngày tải lên: 28/06/2014, 11:20

... involving the secretion of decoy oxidant targets, as well as the synthesis of a broad spectrum of antioxidant enzymes [26,27] Agents with the ability to enhance antioxidant pathways and interfere with ... has the ability to measurably increase antioxidant HO-1 gene expression in the human nasal mucosa and provides a potential mechanistic link between the antioxidant-associated in vitro data and ... studies and assisted in human data analysis CR carried out nasal biopsies and assisted in human subject studies VS carried out or assisted with assays and cell culture work SB and ES conceived of and...

Ngày tải lên: 12/08/2014, 13:22

... index of Basella rubra L was 1.8 µg/mL, profusely lower than garlic acid and ascorbic acid The lower of IC50 the more effective of antioxidant activity, therefore, the antioxidant of Anthocyanin ... Concentration of anthocyanin (µg/mL) Figure Trend line of Basella rubra L anthocyanin antioxidant activity The IC50 index was determined as 1.8 µg/mL, significant low compared to ascorbic acid and garlic ... therefore, the antioxidant activity of Anthocyanin is higher Since the high antioxidant activity, Basella rubra L can be considered as potential sources of functional foods in prevent cancer, anti-inflammatory...

Ngày tải lên: 06/10/2015, 15:58

... Yang J Analysis of the expression of human N-myristoyltransferase (hNMT-1) in cancers Open Biomarkers J 2009; 2: 6-10 22 Graham FL, van der Eb AJ A new technique for the assay of infectivity of ... prostate cancer (Fig 1) Because cancer undergoes metastasis and spreads to other organs at late stages, we speculated that RIZ1 might play an important role in tumor metastasis, although increases ... different types of cancer, and evaluated the anticancer activity of the PR domain against human hepatoma HuH7 cells through both direct administration of recombinant His6-tagged PR and cDNA transfection...

Ngày tải lên: 25/10/2012, 11:15

... localization, and quantification of microbial populations, (2) the determination of physicochemical microenvironment, and (3) the measurement of their in situ activity Combination of FISH and microsensor ... 2004 EXPERIMENTAL MATERIALS AND METHODS Biofilm Samples Two types of biofilms, a domestic wastewater biofilm and an autotrophic nitrifying biofilm, were studied Both biofilms were cultured in partially ... recombinants, and one representative of each group of recombinants was selected for comparative sequence analysis Partial sequencing (ca 465 bp) of the 16S rDNA inserts was performed with an automatic...

Ngày tải lên: 05/09/2013, 08:40

... and complexity of cell wall Silver ion (Ag+) is a bactericidal agent (Pedahzur et al., 1995) The binding of Ag+ and respiratory enzymes initiates changes in permeability of plasma membranes of ... the mean and standard deviation of independent triplicates - 50 - Journal of Water and Environment Technology, Vol.3, No.1, 2005 Increasing the concentration of photocatalysts did not increase ... protein and minerals, explained the loss of cell viability at the early stage of PCO and the appearance of electron-translucent region after 30 of irradiation Cell wall destruction was believed...

Ngày tải lên: 05/09/2013, 09:08

... stage of the parasite Degradation of haemoglobin is performed in the food vacuole by the combined action of aspartic proteases (plasmepsins I, II, IV and histoaspartic protease), cysteine proteinases ... [12,31] To ascertain the inhibition range of MA on the different P falciparum protease classes, in vitro enzymatic assays were performed encompassing cysteine, aspartic, serine and metalloproteases ... and cysteine proteases Remarkably, no inhibition was observed on the aspartic protease pepsin However, a possible specific inhibition of plasmodial aspartic proteases could not be discarded, as...

Ngày tải lên: 14/02/2014, 14:20

... involved in assembly and export of N-oxide reductases Biochem Soc Trans 33, 124–126 29 Zhang B, Zhang Y, Wang Z & Zheng Y (2000) The role of Mg2+ cofactor in the guanine nucleotide 524 exchange and GTP ... glycerol and TMAO and the benzyl viologen-linked TMAO reductase activity of periplasmic fractions was assayed (Fig 7A) TorDD68W was observed to support assembly of the periplasmic TMAO reductase activity ... enhanced XTPase activity (Fig 6A, B) To test for an increased affinity for xanthine nucleotides by a different means, the standard malachite green assay was modified to perform an assay measuring the...

Ngày tải lên: 16/02/2014, 09:20

... tBid–GFP transfected in HEK-293T cells led to increased caspase activity When GFP–Itch was cotransfected with tBid, caspase activity was dramatically reduced (Fig 2B, right panel, Itch) In contrast, ... panel) or lysed and analysed for caspase activity by measuring degradation of the Ac-DEVD-pNA peptide (right panel) The graphs represent average cell survival as a percentage of the control and ... ngÆmL)1 recombinant human TRAIL for h in combination or not with 100 ngÆmL)1 EGF Cell survival was assessed using the MTT assay Caspase activity was assessed by measuring degradation of the Ac-DEVD-pNA...

Ngày tải lên: 16/02/2014, 09:20

... binds between helices and [31] Human increased sodium tolerance-1 (IST1), an ortholog of yeast Ist1, possesses both MIM1 and MIM2 at its C-terminus [32,33] IST1 and Ist1 can bind to several ESCRT-related ... Open triangles and closed triangles indicate bands of recombinant calpain 7MIT and GST, GST–IST1, GST–IST1MIM, and GST–IST1MIML326D,L353A, respectively point mutants, signals were significantly ... use Enhancement of calpain autolysis by IST1 Purification of recombinant human CSD1 was performed as described previously [47] Pulldown assay of GST–MIT–pHis binding to FLAG-tagged CHMPs and IST1...

Ngày tải lên: 18/02/2014, 04:20