quá trình phiên mã rna từ dna

... the DNA/DNA and RNA/DNA oligonucleotide duplexes. In the present study, we determined the temperature- independent and temperature-dependent thermodynamic parameters of 24 DNA/DNA and 41 RNA/DNA ... universally assigning the appropriate thermodynamic parameter sets. In the present study, the 24 DNA/DNA and 41 RNA/DNA oligonucleotide duplexes, designed to avoid the formation of hairpin or slipped duplex ... Temperature dependence of thermodynamic properties for DNA/DNA and RNA/DNA duplex formation Peng Wu 1, *, Shu-ichi Nakano 1 and Naoki Sugimoto 1,2 1 High Technology

Ngày tải lên: 22/02/2014, 07:20

... results exclusively from DNA-PKcs interactions with the DNA We also show that sequence differences in DNA termini can drastically influence DNA repair through altered DNA-PK activation Our results ... (DSBs) 1.3 DNA Damaging Agents in Cancer Therapy 1.4 Ku70/80 1.5 DNA-PKcs 14 1.6 Ku/DNA-PKcs Interactions 16 1.7 DNA/DNA-PK Interactions ... NHEJ non-homologous end joining HR homologous recombination DDR DNA damage response DNA-PK DNA dependent protein kinase DNA-PKcs DNA dependent protein kinase catalytic subunit PIKKs phosphatidylinositol-3

Ngày tải lên: 24/08/2014, 09:33

... for the DNA containing subtle DNA lesions that not arrest the polymerases? DNA repair enzymes are molecular sensors of damaged DNA in the cell, since they recognize and repair damaged DNA It would ... suppression with transcription-coupled DNA repair integrity of the DNA as they are arrested at the bulky DNA lesions inflicted by mutagens (33) while processing along the DNA to carry out their functions ... arrested RNA polymerase at the DNA lesion However, the mutagenic O6-methylguanine (6MG) lesion which is bypassed by RNA polymerase is also preferentially repaired at the transcriptionally active DNA

Ngày tải lên: 16/09/2015, 12:15

... M.SssI, SssI DNA methyltransferase; M.HhaI, HhaI DNA methyltransferase; MTase, DNA methyltransferase; V0, initial rate of methylation; Vmax, maximal rate of methylation FEBS Journal 274 (2007) ... the Kd values of the ternary MTase•(unmethylated cis-B[a]P-DNA)• AdoHcy and MTase•(hemimethylated cis-B[a]P-DNA)• AdoHcy complexes are comparable to the Kd values of the ternary complexes of MTases ... bound 32P-labeled DNA (R) is the ratio of the fraction of bound 32 P-labeled DNA in the presence of the competitor DNA (cpmbound ⁄ cpmtotal) to the fraction of bound 32P-labeled DNA in the absence

Ngày tải lên: 19/02/2014, 00:20

... processes such as DNA synthesis and transcription [26] The bifunctional cisplatin DNA adducts slow down or block DNA or RNA polymerization and can hamper the initiation of DNA transcription [49] ... DNA-binding site FEBS Journal 273 (2006) 4693–4706 ª 2006 The Authors Journal compilation ª 2006 FEBS 4693 ˇ ´ H Pivonkova et al Cisplatin effects on p53 ⁄ p73 DNA recognition DNA binding is underlined ... protein with cisplatin-modified DNA (cisPt-DNA) have recently been studied [31– 36] In the absence of the p53DBS, enhancement of p53 sequence-nonspecific DNA binding due to DNA cis-platination was observed

Ngày tải lên: 19/02/2014, 05:20

... aggregative with aberrant DNA NC SSC Aggregative proteins LD DNA affinity Mono Q Marker NF Separated fractions no DNA relaxed A Aggregation-coupled DNA end-joining (kDa) 225 150 100 75 DNA-PKcs 180 k 170 ... Likewise, DNA-PKcs promoted predominant aggregation of ssDNA when incubated at relatively low concentrations At higher ratios of protein to DNA, DNA-PKcs started to interact with nicked and linear DNA ... 3063–3075 ª 2006 The Authors Journal compilation ª 2006 FEBS B DEAE Sepharose 0.5 M / FT No DNA Human Nuclear Extract Substrate A Aggregation-coupled DNA end-joining Input DNA M Takahagi and K Tatsumi

Ngày tải lên: 19/02/2014, 06:20

... major double-stranded DNA binding determinant in the E. coli DnaE [12]. Crystal structures revealed that this motif binds dou- ble-stranded DNA similarly in both PolC [8] and DnaE [6]. The b-clamp ... and DnaE. The ability to bind sin- gle-stranded DNA has indeed been demonstrated for the E. coli DnaE OB domain [12,16]. The very N-ter- minal region of PolC and the C-terminal domain of DnaE ... subtilis replisome [4] showed that the division of labor between PolC and DnaE is of a different nature. DnaE, much like eukaryotic DNA polymerase a, initially extends an Abbreviations OB, oligonucleotide

Ngày tải lên: 05/03/2014, 23:20

... expansion of DNA repeat sequences is associated with many genetic diseases in humans. Simple bulge DNA structures have been implicated as intermediates in DNA slippage within the DNA repeat regions. ... nucleotide slippage during in vitro DNA synthesis [20]. D. Ouyang et al. Expansion of DNA repeat sequences FEBS Journal 275 (2008) 4510–4521 ª 2008 The Authors Journal compilation ª 2008 FEBS 4511 of ... selected DNA sequences. Solid line, 20 l M free DNA. Dashed line: (A,C,E,G,I) complex of DNA with DDI-1A (50 l M), (B,D,F,H,J) drug-alone has been subtracted. Dotted line: (A,C,E,G,I) complex of DNA with

Ngày tải lên: 07/03/2014, 06:20

... products, including MutS, MutL, MutH, DNA helicase II, single-stranded DNA binding protein, exonuclease I, VII or RecJ exonuclease, DNA polymerase III holoenzyme and DNA ligase [1,7] In E coli, repair ... duplex DNA) were heat denatured with SDS loading buffer, analysed by 10% SDS ⁄ PAGE, followed by silver staining to visualize both proteins and DNA [(C) Heteroduplex DNA (D) homoduplex DNA; lane ... MutS–MutL-DNA complexes are formed by adding duplex DNA to a mixture containing MutS and MutL In experiments involving nucleotide cofactors such as ATP or ATPcS, MutS-DNA (or MutS–MutL-DNA) complexes

Ngày tải lên: 07/03/2014, 12:20

... has a role in domain stability and DNA binding [21] FEBS Journal 274 (2007) 2385–2395 ª 2007 The Authors Journal compilation ª 2007 FEBS 2387 Papillomavirus E2 DNA-binding domain simulations M ... lead to the selection of an alternative binding site FEBS Journal 274 (2007) 2385–2395 ª 2007 The Authors Journal compilation ª 2007 FEBS 2391 Papillomavirus E2 DNA-binding domain simulations ... dynamics; rmsf, root-mean-square fluctuations FEBS Journal 274 (2007) 2385–2395 ª 2007 The Authors Journal compilation ª 2007 FEBS 2385 Papillomavirus E2 DNA-binding domain simulations M Falconi et al

Ngày tải lên: 16/03/2014, 10:20

... U DNA ligase (Weiss units in the case of T4 DNA ligase) at 16 °C (T4 DNA ligase and E.coli DNA ligase) or 45 °C(Taq DNA ligase and H. Kuhn and M. D. Frank-Kamenetskii Ligation of ssDNA by T4 DNA ... or RNA) or protein analytes [9–11], in DNA nanotechnology [12,13], and in DNA computation [14–16]. T4 DNA ligase, the prototype of ATP-dependent DNA ligases [17–19], is the most commonly used DNA ligase. ... Ligation of ssDNA by T4 DNA Ligase FEBS Journal 272 (2005) 5991–6000 ª 2005 FEBS 5995 ligation yields for substrates II ⁄ IV and II ⁄ V, each con- sisting of one ssDNA and one blunt-ended dsDNA terminus,

Ngày tải lên: 23/03/2014, 11:20

... antigen; pol, DNA polymerase. 2984 FEBS Journal 273 (2006) 2984–3001 ª 2006 The Authors Journal compilation ª 2006 FEBS proliferating cell nuclear antigen (PCNA), onto the 3¢ end of the RNA–DNA primer ... Human DNA polymerases a, d and e in replication (1994) DNA replication in vitro by recombinant DNApolymerase-a-primase... step of human chromosomal DNA replication causes DNA ... bold line. Human DNA polymerases a, d and e in replication A. K. Rytko ¨ nen et al. 2986 FEBS Journal 273 (2006) 2984–3001 ª 2006 The Authors Journal compilation ª 2006 FEBS DNA in late S phase

Ngày tải lên: 30/03/2014, 10:20

... noncoding DNA A. Kropotov et al. 2612 FEBS Journal 273 (2006) 2607–2617 ª 2006 The Authors Journal compilation ª 2006 FEBS A B C A. Kropotov et al. Transcription of noncoding DNA FEBS Journal 273 ... indi- cating high efficiency of RNA uptake by A549 cells. RNA extraction, cDNA synthesis, and real-time PCR Total RNA was extracted from A549 cells using Trizol Reagent (Invitrogen). cDNA was synthesized ... 8-oxoG, 7,8-dihydro-8-oxoguanine; PRDX5, peroxiredoxin V; siRNA, small interfering RNA. FEBS Journal 273 (2006) 2607–2617 ª 2006 The Authors Journal compilation ª 2006 FEBS 2607 Mammalian PRDX5 was

Ngày tải lên: 30/03/2014, 11:20

... RT/RNAse H assay does not appear to affect first strand DNA synthesis by RT, but rather, it affects the specificity of cleavage by RNAse H resulting in reduced second strand synthesis from the RNA ... functions as part of the viral DNA replication complex to increase the efficiency of both early and late phase viral DNA synthesis. Published: 15 July 2005 Virology Journal 2005, 2:55 doi:10.1186/1743-422X-2-55 ... citation purposes) Virology Journal Open Access Research Human cytomegalovirus uracil DNA glycosylase associates with ppUL44 and accelerates the accumulation of viral DNA Mark N Prichard* 1 , Heather

Ngày tải lên: 19/06/2014, 08:20

... before DNA extraction) and d indicating DNase treament (the pelleted sample was resuspended and DNase treated before DNA extraction). The > indicates product that was sequenced. Virology Journal ... with modified primers. This assay will not work on RNA viruses and DNA virus types that do not have a DNA dependant DNA polymerase gene such as Hepadna- viridae, Circoviridae, Parvoviridae, Papillomaviridae ... products but the vertebrate DNA polymerase genes con- tain introns making the DNA polymerase products from the host genomic DNA much larger than from viral genomic DNA. The predicted PCR product

Ngày tải lên: 20/06/2014, 01:20

... RT/RNAse H assay does not appear to affect first strand DNA synthesis by RT, but rather, it affects the specificity of cleavage by RNAse H resulting in reduced second strand synthesis from the RNA ... functions as part of the viral DNA replication complex to increase the efficiency of both early and late phase viral DNA synthesis. Published: 15 July 2005 Virology Journal 2005, 2:55 doi:10.1186/1743-422X-2-55 ... citation purposes) Virology Journal Open Access Research Human cytomegalovirus uracil DNA glycosylase associates with ppUL44 and accelerates the accumulation of viral DNA Mark N Prichard* 1 , Heather

Ngày tải lên: 20/06/2014, 04:20

... transcription by T7 RNAP. E denotes free enzyme state; D, DNA template; ED c , DNA-bound closed complex; ED o , DNA-bound open complex; E init D ÀN m , ternary complex engaged in abortive RNA synthesis; ... real-time replication rate of a single DNA polymerase (DNAP) on a single-stranded DNA (ssDNA) stretched by a magnetic trap as shown in Fig. 5A. Similarly to RNAP, DNAP reads the base sequence information ... expression. In their experiments, a ternary complex including DNA tem- plate, RNAP, and nascent RNA is stalled at a predetermined position, followed by immobilization of RNAP onto a bead surface which

Ngày tải lên: 22/06/2014, 18:20

... sites on RNA • • • • • • Comparative RNA web site http://www .rna. icmb.utexas.edu/ RNA world http://www.imb-jena.de /RNA. html RNA page by Michael Suker http://www.bioinfo.rpi.edu/~zukerm /rna/ RNA structure ... tRNA processing snRNA - small nuclear snoRNA - small nucleolar mRNA processing, poly A addition rRNA processing/maturation/methylation regulatory RNAs (siRNA, ... Basis for secondary structure prediction uRNA K1 stRNA1 K2 stRNA2 K1 [ stRNA1] = = exp { ( ∆G2 − ∆G1 ) / RT } K [ stRNA2] K1 = [ sRNA1] = exp{ − ∆G / RT } [ uRNA] ...

Ngày tải lên: 13/03/2014, 17:01

... chiết RNA tổng số, phiên mã ngược tạo cDNA khuếch đại gen DREB5 kỹ thuật PCR; - Tách dòng đọc trình tự gen DREB5 đậu tương; - So sánh trình tự gen DREB5 giống đậu tương nghiên cứu với trình tự ... cấu trúc DREB5 mẻ có công trình Chen M cs (2007) Xuất phát từ lí tiến hànhđề tài luận văn thạc sĩ là: “Nghiên cứu gen DREB5 mã hóa protein liên quan đến trình phiên mã nhóm gen chịu hạn đậu tương ... LUẬN 4.3 Kết phân lập gen DREB5 từ đậu tương 4.3.1 Tách chiết RNA từ mầm giống đậu tương M RNA Hình 4.5 Ảnh điện di RNA tổng số gel agarose 1% Ghi : M: thị phân tử DNA thực khuẩn thể l cắt HindIII...

Ngày tải lên: 22/04/2013, 11:57

... III QUÁ TRÌNH PHIÊN MÃ Khái niệm: Là trình truyền thông tin di truyền từ phân tử ADN mạch kép sang ARN mạch đơn (sgk Sinh 12 nâng cao) Quá trình có nhiều tên gọi: phiên mã, tổng hợp ARN, mã ... động kiểm soát trình phiên mã - Vùng mã hóa: Mang thông tin mã hóa a.a - Vùng kết thúc: Mang tín hiệu kết thúc phiên mã Vùng điều hòa Trong vùng mã hóa có đoạn thực mang thông tin mã hóa a.a (gọi ... phiên mã, tổng hợp ARN, mã Định nghĩa nghĩa tất đoạn ADN phiên mã trở thành ARN Chỉ có gen (định nghĩa phía trên) phiên mã Quá trình phiên mã xảy mạch gen, mạch gọi mạch gốc Yếu tố tham gia - Enzim:...

Ngày tải lên: 21/01/2014, 13:20