purification of his tagged proteins under denaturing conditions on ni nta agarose
simple and rapid synthesis of a-fe2o3 nanowires under ambient conditions
Ngày tải lên :
20/03/2014, 13:07
... direction and contained stacking faults along the NW direction A mechanism of NW growth based on the diffusion of iron ions to the surface of wire through grain boundaries and to the tip of the ... heating of an iron wire is about six orders of magnitude lower and thereby cannot significantly affect the ion motion across the wire The iron oxidation process involves iron ion diffusion from ... growth of the NWs Figure shows our understanding of the NW formation conditions and a suggested mechanism for their growth on the basis of our experimental results and literature data The formation...
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Báo cáo lâm nghiệp: " Growth and mineral content of young chestnut trees under controlled conditions of nutrition" ppsx
Ngày tải lên :
08/08/2014, 18:22
... disorganization of clay colloids, which increases ion availability The evolution of Mg concentrations was the reverse: a decrease was observed during the experimentation time under all conditions, ... sets of conditions, A, B, C, D and O A consisted of a single supply of quick-lime /kg 2+ Ca of soil, corresponding to -1 kg.ha (94.0% CaO) for forest fertiliza- (2 cmolc of 000 tion); B consisted ... consideration the K level in leaves (0.87%) under optimal nutritional conditions (Colin-Belgrand et al, 1993) In addition, the concentration of each elevaried differently within each component over...
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Impacts of Changes in Policy and Market Conditions on Land Use, Land Management and Livelihood Among Farmers in Central Highlands of Vietnam
Ngày tải lên :
13/08/2015, 10:01
... As a result of the rapid expansion of coffee production, coffee landed as one of the major export crops of the country In 1999, production of coffee green beans rose to 439, 000 tons, the highest ... decollectivization of agriculture after 1988 The key feature of the decollectivization policies are: (1) Recognition of the farm household as the main unit of agricultural production, (2) Liberalization of ... operation of the state farm mainly concentrates on providing technical service to support sericulture production of individual households and collection of land tax The personnel of this state...
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Surface chemistry of organic carbonyl compounds and their derivatives on ni (111
Ngày tải lên :
17/09/2015, 17:19
... Adsorption of DMP on Clean Ni( 111) 147 7.2.1.2 Adsorption of DMP on Ni( 111)-p(2×2)-O 149 7.2.1.3 Adsorption of DMP on Ni( 111)-0.1ML-O 151 7.2.2 Adsorption of DME on Ni( 111) 152 7.2.2.1 Adsorption of ... Introduction 64 4.2 Results 66 4.2.1 Adsorption of Acetone on Preoxidized Ni( 111) at 120K 66 4.2.2 Adsorption of Acetone on Ni( 111)-p(2×2)-O between 180-340K 66 4.2.3 Adsorption of Acetone on Ni( 111)-0.1ML-O ... Adsorption of AcacH on Ni( 111)-0.1ML-O 92 5.3 Discussion 93 5.3.1 Coordination Modes of Adsorbed AcacH on Ni( 111) 93 5.3.2 Decomposition Mechanism of Adsorbed AcacH on Ni( 111) 94 5.4 Conclusions...
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iec 60332-3-24 tests on electric cables under fire conditions - test for vertical flame spread of
Ngày tải lên :
25/12/2013, 11:00
... organizations 2) The formal decisions or agreements of the IEC on technical matters express, as nearly as possible, an international consensus of opinion on the relevant subjects since each technical ... international co-operation on all questions concerning standardization in the electrical and electronic fields To this end and in addition to other activities, the IEC publishes International Standards ... l'échantillon d'essai et l'intérieur des montants de l'échelle doit être au minimum de 50 mm Lors du montage des tronçons de câble, le premier tron on ou la premiere rangée de tronçons de cables...
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iec 60332-3-25 tests on electric cables under fire conditions - test for vertical flame spread of
Ngày tải lên :
25/12/2013, 11:01
... other restrictions on the selection of cables A summary of all conditions for type approval testing to this part of IEC 60332 is given in table A.l Table A.l'- Summary of test conditions I I Category ... organizations 2) The formal decisions or agreements of the IEC on technical matters express, as nearly as possible, an internatlonal consensus of opinion on the relevant subjects since each technical ... documents contain provisions which, through reference in this text, constitute provisions of this part of IEC 60332 For dated references, subsequent amendments to, or revisions of, any of these...
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Tài liệu Báo cáo khoa học: The yeast ubiquitin ligase Rsp5 downregulates the alpha subunit of nascent polypeptide-associated complex Egd2 under stress conditions docx
Ngày tải lên :
18/02/2014, 11:20
... ubiquitination of denatured proteins under stress conditions [32–34] In addition, identification of ubiquitinated forms of mis-folded proteins by HECT-type ubiquitin ligase under stress conditions has ... compilation ª 2009 FEBS 5293 Ubiquitination of Egd2 by Rsp5 under stress conditions H Hiraishi et al Table Oligonucleotides used in this study The underlining indicates the sequence upstream of the initiation ... are produced under stress conditions, but that little ubiquitination of the native forms of Egd2 occurs under non-stress conditions We also found that the stability of Egd2 was decreased in wild-type...
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![Tài liệu Báo cáo khoa học: Expression of two [Fe]-hydrogenases in Chlamydomonas reinhardtii under anaerobic conditions doc](https://media.store123doc.com/images/document/14/br/hw/medium_hwm1392870031.jpg)
Tài liệu Báo cáo khoa học: Expression of two [Fe]-hydrogenases in Chlamydomonas reinhardtii under anaerobic conditions doc
Ngày tải lên :
20/02/2014, 11:20
... were performed under strictly anaerobic condition The hydrogenase-containing fraction was partially purified as described previously [29] and run on SDS/PAGE under denaturing conditions with a 10% ... deprivation The expression of HydA1 and HydA2 transcripts was also studied under sulfur-deprivation conditions In the absence of sulfur, the rates of photosynthetic O2 evolution drop below those of ... 4.62 kb and consists of eight exons and nine introns (average intron size, 150 bp) The structural arrangement of HydA2 is thus more complex than HydA1, which contains only seven introns [16] Interestingly,...
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Báo cáo Y học: Overexpression of a recombinant wild-type and His-tagged Bacillus subtilis glycine oxidase in Escherichia coli pptx
Ngày tải lên :
08/03/2014, 16:20
... lysis of 34 g of E coli cells under the conditions reported above, was precipitated with ammonium sulfate at 30% of saturation (164 gÆL)1) The supernantant was then brought to 45% of saturation ... for GO activity Protein concentration was measured using the Bradford protein assay Cloning of B subtilis GO cDNA and DNA manipulation The cloning and transformation techniques used were essentially ... and mM EDTA The fractions containing GO activity were concentrated and stored at )20 °C HisGO preparation, expression and purification The GO DNA obtained by EcoRI digestion of the original pT7-GO...
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Báo cáo khoa học: Response of the Pacific oyster Crassostrea gigas to hypoxia exposure under experimental conditions pot
Ngày tải lên :
16/03/2014, 23:20
... Expression of carbonic anhydrase in gills (B) Expression of glutathione peroxidase in mantle (C) Expression of myc homologue in gills (D) Expression of BTF3 in mantle (E) Expression of glycogen ... Threonine 3-dehydrogenase Putative 28 kDa protein, partner of Nob1 ATP synthase alpha subunit Protein regulation Translation elongation factor 1-alpha Elongation factor Reproduction Vitellogenin ... 390–403 27 Boutet I, Tanguy A & Moraga D (2004) Response of the Pacific oyster Crassostrea gigas to hydrocarbon contamination under experimental conditions Gene 329, 147–157 28 Bultelle F, Panchout...
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Báo cáo khoa học: The complex of the insect LDL receptor homolog, lipophorin receptor, LpR, and its lipoprotein ligand does not dissociate under endosomal conditions pot
Ngày tải lên :
23/03/2014, 07:20
... 342)LDLR(293–839) contains Ile313, it does not contain the complete hinge region of LDLR The presence of two Gly residues in LpR at the corresponding positions of His2 64 and Ser265 of LDLR (Table ... in lipid content may trigger HDLp resecretion The stability of the complex and the modulation thereof may be determined by secondary contacts between HDLp and nonconserved residues of LpR Although ... endosomal conditions fail to result in dissociation of the complex, signifying that HDLp and LpR travel in complex to the ERC Experiments using an LpR–LDLR hybrid receptor containing LA-1–7 of LpR...
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Báo cáo khoa học: Mechanism of the ring contraction process in vitamin B12 biosynthesis by the anaerobe Propionibacterium shermanii under aerobic conditions docx
Ngày tải lên :
23/03/2014, 09:20
... Iida et al Ring contraction mechanism of vitamin B12 Fig The mechanisms of the ring contraction process in the biosynthesis of vitamin B12 postulated on the basis of the isolation of factor IV from ... migration of ring D followed by cleavage of the carbon–oxygen bond of the c-lactone at C1 of 13C,18O-precorrin3x [8–10] as shown in the lower row of Fig We were interested in the mechanism of corrin ... Ring contraction mechanism of vitamin B12 Fig The highlights of previously postulated mechanisms of the ring contraction process in the biosynthesis of vitamin B12 in the anaerobe P shermanii (top...
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Báo cáo khoa học: Large-scale overproduction, functional purification and ligand affinities of the His-tagged human histamine H1 receptor ppt
Ngày tải lên :
30/03/2014, 14:20
... scintillation counting This dual effect of both stabilizing and estimating functional receptors greatly facilitated the optimization of conditions for solubilization, purification and reconstitution For ... interactions of the histamine receptors Elucidation of the structure and dynamics of membrane proteins is a challenging task essential for proper understanding of the functioning of fundamental biological ... agonist affinities may significantly depend on interactions or the microenvironment of GPCR, a tentative explanation may be that the lower fluidity of the Sf9 cell membrane positively affects agonist...
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assessment of the stress and strain levels at geometrical discontinuities under elastoplastic conditions a case study
Ngày tải lên :
24/05/2014, 20:32
... reason of the magnitude of its hardening exponent and strength coefficient On the other hand, the decrease of the stress concentration factor Kσ after yielding and the increase of the strain concentration ... and it was refined on the regions of interest This model was used to determine the stress distribution on two specific points of the discontinuity geometry, on the surface and on the mid thickness ... finite element three dimensional model for 100 MPa of nominal stress in the geometrical discontinuity section: (a) σxx, (b) σyy e (c) σzz Analysis of the two dimensional finite element model considering...
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Báo cáo sinh học: " Purification of infectious human herpesvirus 6A virions and association of host cell proteins" pot
Ngày tải lên :
18/06/2014, 18:20
... purified HHV-6A virion samples Results HHV-6A production During purification and characterisation of virions, a major obstacle is contaminations of the viral preparations The contaminations can stem ... if the virions were harvested in culture media containing 10% serum (data not shown) We concluded that the purification of HHV-6A virions removed a substantial amount of cellular contaminating ... association of these cellular proteins with purified HHV-6A virions This was confirmed by quantifications of at least two Page of 11 (page number not for citation purposes) http://www.virologyj.com/content/4/1/101...
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Báo cáo hóa học: " Purification of infectious human herpesvirus 6A virions and association of host cell proteins" potx
Ngày tải lên :
20/06/2014, 01:20
... purified HHV-6A virion samples Results HHV-6A production During purification and characterisation of virions, a major obstacle is contaminations of the viral preparations The contaminations can stem ... if the virions were harvested in culture media containing 10% serum (data not shown) We concluded that the purification of HHV-6A virions removed a substantial amount of cellular contaminating ... association of these cellular proteins with purified HHV-6A virions This was confirmed by quantifications of at least two Page of 11 (page number not for citation purposes) http://www.virologyj.com/content/4/1/101...
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Báo cáo hóa học: " Bacterial-based systems for expression and purification of recombinant Lassa virus proteins of immunological relevance" doc
Ngày tải lên :
20/06/2014, 01:20
... detection and rapid implementation of existing treatment regimens (e.g ribavirin administration) The lack of adequate countermeasures and means of detection, coupled with the severity of disease, contributed ... vector contains a mutation in the translocation signal and thus will yield only cytoplasm-associated recombinant proteins Selection of vector pMAL-c2x for expression of LASV NP, GP1, and GP proteins ... higher concentrations of NP per unit of cell mass were achieved with pMAL-c2x when compared to the -p2x counterpart, a significant portion of the protein was contained in insoluble fractions after...
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Báo cáo lâm nghiệp: "Variation of the tree form factor and taper in European larch of Polish provenances tested under conditions of the Beskid Sądecki mountain range (southern Poland)" docx
Ngày tải lên :
07/08/2014, 10:22
... be understood as conditions on a macro scale Specific growth conditions on a micro scale, occurring in individual experimental plots of the provenance experiment and determined on the basis of ... spite of a certain diversification of mean stem profiles of individual partial populations, no significant effect of the genotype (provenance) on their variation was found The variation of the ... variability of Abies grandis stem form under mountain conditions of Beskid Sądecki (southern Poland) Canadian Journal of Forest Research, 35: 1–14 StatSoft, Inc 2004 STATISTICA, version www.statsoft...
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Báo cáo lâm nghiệp: "Phenological investigations of different winter-deciduous species growing under Mediterranean conditions" pot
Ngày tải lên :
07/08/2014, 16:21
... during 2001 one plant of S acutifolia had some problems; so it was substituted by the one of two remaining plants of the same age present in the garden and this new plant has been monitored since ... evidence the significance level of the predictions RESULTS Generally, for the different species considered in this study the phenological phases corresponding to the beginning of growing season (phases ... decoupling of species interactions While, on the other hand, in a warming scenario the lengthening of plant growing season could alter the structure and functioning of plant communities t value P(> |t|)...
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