purification in vitro translation and gst pulldowns
Báo cáo y học: " Interactions between Nef and AIP1 proliferate multivesicular bodies and facilitate egress of HIV-1" potx
Ngày tải lên :
13/08/2014, 09:20
... inputs Figure AIP1 in vitro and invivo Nef binds Nef binds AIP1 in vitro and invivo.(A)Nef binds AIP1 in vitro GST and GST. AIP1 fusion proteins were expressed in E coli and purified by glutathione ... 3:33 Protein purification, in vitro translation and GST pulldowns The GST. AIP1 fusion protein was expressed in the BL21(DE3)pLysS strain of E coli (Novagen, Madison, WI) and purified using Glutathione ... of SIV replication in rhesus macaques [49] Nef binds AIP1 in vitro and in vivo Next, we investigated the ability of Nef to bind AIP1 To detect this binding, plasmids directing the expression...
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in vitro transcription and translation protocols
Ngày tải lên :
11/04/2014, 09:47
... radioactivity indicated here, you should maintain identical ratios in first- and second-strand syntheses Our protocol results in ds cDNA of an SA of 6.67 x lo5 dpm/l.tg Single-stranded cDNA (first-strand ... be inhibited in the presenceof antisense RNA in vitro (14), and in vivo translation has been blocked in Xenopus oocytes by antisense RNA (15) The ability to synthesize discrete RNA templates in ... whose inserts are unidirectional and in opposite orientation to each other (seeFig 1) In this way, the induced single-strandphage DNA will contain vectors of the same polarity (hence,nonhybridizing)...
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Báo cáo sinh học: " Stimulation of poliovirus RNA synthesis and virus maturation in a HeLa cell-free in vitro translation-RNA replication system by viral protein 3CDpro" docx
Ngày tải lên :
19/06/2014, 08:20
... both the proteinase and the RNA binding site [25,26] While the primary RNA binding determinant of 3CDpro lies in 3Cpro, lower affinity binding determinants are located in the 3Dpol domain [27,28] ... The viral proteins most directly involved in RNA replication include protein 3AB, the precursor of 3A, which is a small membrane binding and RNA binding protein, the terminal protein VPg, RNA polymerase ... production in vivo and in vitro In the in vitro system a large amount of viral RNA (~1 × 1011 RNA molecules) has to be used, as template for translation and replication, in order to obtain infectious...
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Báo cáo khoa học: "Stochastic Language Generation Using WIDL-expressions and its Application in Machine Translation and Summarization" pot
Ngày tải lên :
23/03/2014, 18:20
... Franz Josef Och 2003 Minimum error rate training in statistical machine translation In Proceedings of the ACL, pages 160167 Kishore Papineni, Salim Roukos, Todd Ward, and WeiJing Zhu 2002 BLEU: ... rebels ghting 0.130 in iraq attacked rebels turkish goverment D VC rebels ghting turkish government in iraq traversal of , starting from and ending in Each path (and its associated string) has ... states and in exists if there exists a vertex in the description of and a vertex in the description of such that there exists a path in between and , and is the only -labeled transitions in this...
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Báo cáo khoa học: In vitro selection and characterization of a stable subdomain of phosphoribosylanthranilate isomerase potx
Ngày tải lên :
30/03/2014, 20:20
... variants identified in rounds and 3, and 20 of the 38 identified in round 4) In every case, we observed a frameshift caused by insertion of a single thymine nucleotide into the fourth randomized codon ... (b1) and Gln332 (b4), remain in trPRAI It is tempting to speculate that, in the absence of the salt bridge linking Lys258 and Asp425 (b8), the hydrogen bond donor Lys258 could instead be involved ... amino-terminal autonomous folding unit controls several rate-limiting steps in the folding of a single domain protein Biochemistry 38, 10205–10214 ´ ´ 45 Soberon X, Fuentes-Gallego P & Saab-Rincon...
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Báo cáo hóa học: " In Vitro Structural and Functional Evaluation of Gold Nanoparticles Conjugated Antibiotics" doc
Ngày tải lên :
22/06/2014, 06:20
... by inhibiting the cross-linking reaction mediated by transpeptidase, while both streptomycin and kanamycin bind with ribosome and block translation process during protein synthesis [32] The binding ... of ampicillin, streptomycin and kanamycin along with their respective Gnps conjugated forms against E coli DH5a, M luteus and S aureus in Luria-Bertani (LB) broth were determined by standard method ... seeding of Gnps was done in presence of the antibiotics (Ampicillin, Streptomycin and Kanamycin, Fig 1) individually and thus Gnps conjugated antibiotics were formed [27] Dynamic Light Scattering...
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Báo cáo hóa học: " In Vitro Structural and Functional Evaluation of Gold Nanoparticles Conjugated Antibiotics" pdf
Ngày tải lên :
22/06/2014, 19:20
... by inhibiting the cross-linking reaction mediated by transpeptidase, while both streptomycin and kanamycin bind with ribosome and block translation process during protein synthesis [32] The binding ... of ampicillin, streptomycin and kanamycin along with their respective Gnps conjugated forms against E coli DH5a, M luteus and S aureus in Luria-Bertani (LB) broth were determined by standard method ... seeding of Gnps was done in presence of the antibiotics (Ampicillin, Streptomycin and Kanamycin, Fig 1) individually and thus Gnps conjugated antibiotics were formed [27] Dynamic Light Scattering...
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Báo cáo khoa học " In vitro cytostatic and immunomodulatory properties of the medicinal mushroom Lentinula edodes " pdf
Ngày tải lên :
28/06/2014, 11:20
... spectra (in KBr) of lentinan samples and the immunologically active b-glucan from Saccharomyces cerevisiae The arrows indicate absorption bands typical of lipids (a and b), and proteins (c and d) ... mitogens in vitro, indicating another mechanism for immunostimulatory activity Overall there seems to be a therapeutic advantage in using L edodes extracts orally administered instead of a single ... containing 24.6% protein and 44% sugars, comprising mostly pentoses as well as glucose and smaller amounts of galactose, mannose and fructose (Iizuka, 1986; Sugano et al., 1982) It also contains...
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Báo cáo khoa học: "In vitro neuronal and osteogenic differentiation of mesenchymal stem cells from human umbilical cord blood" ppt
Ngày tải lên :
07/08/2014, 18:21
... ,etahpsohp lorecylg -β fo detsisnoc taht muidem noitcudni cinegoetso na desu ew ,sCSM eht morf noitaitnereffid cinegoetso ecudni oT laitnetop cinegoetso tibihxe sCSM doolb droC )A2 giF( nitnemiv dna ... ecnanetniam eht dna euqinhcet reporp eht ,erutluc yramirp ot gnilpmas morf emit eht taht thguoht saw tI ]72,22[ srotinegorp citeiopotameah rof esac eht sa emas eht ,srotinegorp lamyhcnesem ni ... ladiobuc wohs ton did sCSM BCU eht ,sCSM worram enob ekilnu ,revewoH )4 giF( )gniniats assoK-noV yb deifitnedi( laitnetop cinegoetso evah sCSM taht gnitseggus ,etitapayxordyh demrof sCSM BCU ehT enosahtemaxed...
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Báo cáo y học: "The Short Anxiety Screening Test in Greek: translation and validation" ppsx
Ngày tải lên :
08/08/2014, 23:21
... following reasons: it is short and easy to apply in clinical settings and it is based on an interviewer-assisted self-rating scale, rendering it practical for use in everyday practice According ... Screening Test (SAST) was developed to provide clinicians with a simple tool for detecting anxiety disorders in older people It was developed and standardised in 1999 by Sinoff et al [24] and was ... contribution in the forward and backward translations, and Mrs Adelais Markaki, Dr Sue Shea and Dr Paulos Theodorakis for their advice and consultation Author details Clinic of Social and Family Medicine,...
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Báo cáo y học: "Induction of HSPA4 and HSPA14 by NBS1 overexpression contributes to NBS1-induced in vitro metastatic and transformation activity" potx
Ngày tải lên :
10/08/2014, 05:21
... assay of in vitro migration and invasion activity The results showed that knocking down HSPA4 or HSPA14 in H1299NBS1 cells caused a significant decrease in the in vitro migration and invasion ... transition in head and neck cancer cell lines [17] We wanted to test whether NBS1 overexpression could also increase in vitro migration and invasion activity in a lung cancer cell line NBS1 expression ... overexpression in H1299 cells increased in vitro migration and invasion activity and induced the expression of HSPA4 and HSPA14 We previously demonstrated that NBS1 overexpression induces epithelial-mesenchymal...
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báo cáo khoa học: " Characterization and isolation of a T-DNA tagged banana promoter active during in vitro culture and low temperature stress" ppt
Ngày tải lên :
12/08/2014, 03:20
... the luc+ gene in lines 156, 49 and 111 In addition, T-DNA tandem repeats were identified in lines 156 and 49, and vector backbone sequences were integrated in lines 17, 156, 49 and 179 (data ... I in this line The real-time observations strongly indicate a developmental regulation of the tagged promoter(s) in this line In contrast, all other lines, including the positive control line ... 8°C and 26°C (excluding the images acquired during the transition phase) indicated for line 17 a 10.7fold and a 2.5-fold increase in LUC activity when lowering the temperature at stage I and...
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Báo cáo khoa học: "Tumor slices as a model to evaluate doxorubicin in vitro treatment and expression of trios of genes PRSS11, MTSS1, CLPTM1 and PRSS11, MTSS1, SMYD2 in canine mammary gland cancer" pptx
Ngày tải lên :
12/08/2014, 18:22
... Veterinaria Scandinavica 2008, 50:27 http://www.actavetscand.com/content/50/1/27 Patients were evaluated by clinical history and physical examination including mammary tumor measurement and inguinal ... prognosis in canine mammary tumors [5,6], and immunohistochemical detection of Bcl2, p53 and cytokeratins, in human and canine tumors and corresponding adjacent tissues, have been similar [8] In dogs, ... Acta Veterinaria Scandinavica 2008, 50:27 Introduction Human and canine malignant mammary tumors share some epidemiological and clinicopathological features Incidence in both species increases...
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Engineering and characterization of human renal proximal tubular cells for applications in vitro toxicology and bioartifical kidneys
Ngày tải lên :
09/09/2015, 10:19
... with recombinant BMP-2 and recombinantBMP-7 34 3.8 Treatment with human recombinant TGF-β1 and human recombinant A2M 35 3.9 Immunostaining and quantification of fluorescence intensities ... by immunostaining 58 Figure 5: Double-immunostaining of E-CAD and N-CAD 59 Figure 6: (A) GGT and LAP activity in isolated and commercial HPTC (B) AP activity in isolated and commercial ... might interact with other cells in the renal cortex in producing or responding to costimulatory cytokines, i.e tumour necrosis factor alpha(35) In addition, PTC produce interleukin-6 in response...
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Báo cáo y học: " Preparation of RGD-modified Long Circulating Liposome Loading Matrine, and its in vitro Anti-cancer Effects"
Ngày tải lên :
26/10/2012, 08:57
... phosphate-buffered saline (PBS), and resuspended at × 106 cells /mL in Annexin-V binding buffer The supernatant (100 μL/tube) was incubated with μL of Annexin-V-FITC (Invitrogen, USA) and μL of PI (Invitrogen, ... (GIBCO, USA) containing 10% heat-inactivated fetal bovine serum (Nuoding, China), and the HT-29 cells were grown in DMEM medium (GIBCO, USA) also containing 10% heat-inactivated fetal bovine serum All ... then evaluated by in vitro testing in human cancer cell lines We observed that 198 RGD-M-LCL had increased anti-proliferative activity and increased the induction of apoptosis in cancer cells compared...
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Analysis, sequencing and in vitro expression of PCR products
Ngày tải lên :
25/10/2013, 22:20
... functional domain mapping of proteins where fragments that represent protein domains are expressed and functionally analyzed using in vitro assays Furthermore, rapid in vitro protein–protein interaction ... separation in thin-coated capillaries containing nonpolymerized gel matrices and laser detection systems The introduction and removal of polymer from the capillaries, plus loading and running samples and ... nonbiotinylated single strand Both the strands can be used as templates for DNA sequencing reactions The immobilized strand can be stored and reused for further reactions into solution and leaving...
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Tài liệu Báo cáo khoa học: Proteolysis of Pseudomonas exotoxin A within hepatic endosomes by cathepsins B and D produces fragments displaying in vitro ADP-ribosylating and apoptotic effects doc
Ngày tải lên :
18/02/2014, 04:20
... EF-2 in the mitochondrial apoptotic response induced by the toxin remains to be determined Finally, we assign an important role to the endosomal acidic cathepsins B and D in increasing the in vitro ... death (5 postinjection), suggesting a high binding capacity of ETA in hepatic parenchyma Indeed, injection of the toxin into mice has been shown to result in an early and profound inhibition of ... endosomes containing in vivo internalized ETA The endosomal fraction was isolated 30 after the administration of ETA, and then resuspended in 0.15 M KCl containing mM MgCl2 and, when indicated, 50...
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Tài liệu Báo cáo khoa học: The endogenous retinoid metabolite S-4-oxo-9-cis-13,14-dihydro-retinoic acid activates retinoic acid receptor signalling both in vitro and in vivo pdf
Ngày tải lên :
18/02/2014, 08:20
... ⁄ v) fetal bovine serum (Gibco Invitrogen), 1% (v ⁄ v) PEST (Gibco Invitrogen), 1% (v ⁄ v) l-glutamine (Gibco Invitrogen) and 1% nonessential amino acids (Gibco Invitrogen) Murine hepatoma-1 ... grown in low-glucose DMEM (Gibco Invitrogen) supplemented with 10% (v ⁄ v) fetal bovine serum (Gibco Invitrogen), 1% (v ⁄ v) PEST (Gibco Invitrogen), 1% (v ⁄ v) l-glutamine (Gibco Invitrogen) and ... all-trans-retinoic acid, the findings clearly demonstrate that S-4o9cDH-RA has the capacity to bind and activate nuclear retinoid receptors and regulate gene transcription both in vitro and in vivo Retinoids...
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Tài liệu Báo cáo khoa học: Template requirements and binding of hepatitis C virus NS5B polymerase during in vitro RNA synthesis from the 3¢-end of virus minus-strand RNA docx
Ngày tải lên :
20/02/2014, 01:20
... template and binding properties of mutated and deleted RNA fragments of the 3¢-end of the minus-strand HCV RNA in further detail This study should lead to interesting interpretations since it ... domain in the 3¢-end of the HCV minus-strand RNA inhibits in vitro initiation of RNA synthesis by the viral polymerase [19] Kashiwagi et al [20] studied RNA synthesis by recombinant HCV NS5B using ... unavailable In the present study we investigated the involvement of sequences and ⁄ or structures in RNA synthesis directed in vitro by HCV NS5B by using new mutants of the 3¢-end of minus-strand RNA...
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