purification and characterization of pna amphiphiles

... thankful to Professor Les Copeland (Faculty of Agriculture, Food and Natural Resources - The University of Sydney, New South Wales, Australia) and Professor Kaeko Kamei (Department of Biomolecular ... 37 and 70°C for hours, and the remaining activity (expressed as % of the activity at 37°C) was determined after 30, 60, 120 and 180 minutes Determination of optimal pH and pH-tolerance Page of ... proteins Generally, the two-step chromatography of AEX and HIC was sufficient for the purification of FIII-1, FIII-2, and FII, since their SEC and SDS-PAGE profiles represented pure proteins Fraction...

Ngày tải lên: 20/06/2014, 23:20

... citatsomreht a ot detcennoc )nedewS ,aicamrahP( tinu IIrohpitluM eht ni demrofrep saw sisylana citerohportcele ehT la te gnisucof cirtceleosi lacitylanA snoitacifidom thgils htiw ]42[ nibwoT fo dohtem ... ,BKL( rettolb yrd-imes tolBavoN a gnisu demrofrep saw sisylana tolbonummI la te gnittolbonummI ]31[ ilmmeaL yb debircsed snoitidnoc eht ot gnidrocca demrofrep saw siserohportcele dna ,selpmas ,sleg ... noitaraperP )ASU ,daRoiB( lleC balS-lauD II naetorP -iniM eht ni demrofrep saw sisylana citerohportcelE EGAP-SDS ]3[ dohtem drofdarB eht yb detamitse saw sPLG eht fo tnetnoc nietorp ehT fo sPLG...

Ngày tải lên: 07/08/2014, 20:23

... purification of herpes simplex virus type UL4 protein, and production and characterization of UL4 polyclonal antibody J Virol Methods 2010, 163:465-469 Reed LJ, Muench H: A simple method of estimating ... Expression and characterization of the UL31 protein from duck enteritis virus Virol J 2009, 6:1 doi:10.1186/1743-422X-7-241 Cite this article as: Zhang et al.: Production, purification and characterization ... http://www.virologyj.com/content/7/1/241 Page of Figure Indirect immunofluorescent microscopy was used to monitor the DEV antigen distribution in liver, harder’s glands, cecum, spleen and kidney of the infected ducks...

Ngày tải lên: 12/08/2014, 01:21

... diagnostics and epidemiologic surveys Methods Strains, plasmids, and enzymes Expression and purification of LSV coat protein Figure Expression and purification of LSV coat protein A, Expression and purification ... Alignment of the amino acid sequence of coat protein of different LSV isolates and other carlavirus Figure Alignment of the amino acid sequence of coat protein of different LSV isolates and other ... Laboratory of Arid and Grassland Agroecology of Ministry of Education, School of Life Sciences, Lanzhou University, Lanzhou 730000, China and 4Department of Bacteriology, Graduate School of Medical Science,...

Ngày tải lên: 12/08/2014, 04:21

... polysaccharide One milligram of the partially purified polymer contained 309.7 µg of protein, 160.2 µg of neutral sugar, 58.5 µg of hexosamine, 30.8 µg of uronic acid and 3.8 µg of phosphorus Constituent ... concentration of ppm to estimate the effect of pH After min, A660 was measured and the extent of flocculation was calculated Pronase digestion of extracellular polymer Forty milligrams of the extracellular ... this study were of reagent grade Results and Discussion Extraction and partial purification of extracellular polymer The floc was satisfactorily deflocculated within 10 by sonication of the activated...

Ngày tải lên: 05/09/2013, 09:08

... diameter of the PVDF membrane, the PVDF-PS membrane and the PVDF-SPS membrane is 0.25 µm, 0.12 µm and 0.16 µm, respectively It can be seen that the polymerization of styrene and the blending of PS and ... International Journal of Energy and Environment (IJEE), Volume 1, Issue 4, 2010, pp.643-656 645 2.5 Measurement of proton conductivity The proton conductivity of the PVDF-SPS membrane and Nafion-117 ... compartment (mol.L-1) VB is the volume of right reservoir (cm3) A and L are the membrane cross-sectional area and thickness, respectively α is the slope of the linear plot of methanol concentration in...

Ngày tải lên: 05/09/2013, 16:11

... ordering process and the cost of synthesis is relatively cheap An example of appropriate design of primers to add attB1 and B2 sites to the 5′ and 3′ ends respectively of a gene of interest are ... N-glycosidic bonds between uracil and deoxyribose leading to a loss of the base The loss of bases leads to disruption of base pairing and therefore exposure of single strands that can be annealed to ... ethidium bromide and UV irradiation for detection of DNA in gels, thereby preventing DNA damage of the long molecules and enhancing isolation of full-length clones The range of cloning kits and vectors...

Ngày tải lên: 25/10/2013, 22:20

... system is capable of producing off-resonance, mirror, and flat magnetic field configurations A mirror ratio of 1·1 and a maximum flat field of 1400 G has been obtained The measured values and the design ... varying the size of the solenoid coils, thickness of iron jacket, and amp-turns (NI) The optimum values of NI for the side and middle solenoid coils were obtained to be 12950 and 8250 respectively ... μS/cm) having inlet temperature of 27◦ C Based on the length of the conductor, water pressure drop and flow, the inlet and outlet connections were provided Total five inlet and five outlet connections...

Ngày tải lên: 22/12/2013, 08:58

... evaporation of solvents and volatile products, took place simultaneously with the deposition process, caused the porosity of the TiO2 films Prepareation of TiO2/SnO2: After the material characterization ... concentration of the SnCl4 solution, and the films were annealed at different temperatures and for different period of time Photoconductivity measurement In order to evaluate properties of obtained ... resistance (RD) and the light resistance (RL) under the irradiation of 7W Hg lamp at the distance of 10cm were measured The calculated ratio of RD/RL was considered as a photoconductivity of obtained...

Ngày tải lên: 13/02/2014, 04:20

... tissue of mouse and spleen and thymus of human and mouse [11,37] Foxp3 is also highly expressed in lymphoid organs, such as the thymus and spleen [49], and its expression within a variety of other ... t-bet, stat6 and foxp3 were also determined and found to be very similar to their human homologues Both the human and zebrafish T-bet and Foxp3 genes contained the same number of exons and introns ... the expression of these genes within humans and mice, of T-bet within Ginbuna crucian carp [27] and STAT6 in mandarin fish [28] have shown similar widespread expression In the case of STAT6, highest...

Ngày tải lên: 16/02/2014, 09:20

... Identification of the initiation codons in GmPDIL-3a and Gm-PDIL-3b mRNAs (A) Schematic representation of the structure of GmPDIL-3a and GmPDIL-3b mRNAs The putative ORFs (gray boxes) of GmPDIL-3a and GmPDIL-3b ... S1 Comparison of intron–exon structures of GmPDIL-3a, GmPDIL-3b and orthologs of other plants Fig S2 Multiple amino acid sequence alignment of GmPDIL-3a, GmPDIL-3b and orthologs of other plants ... and CD analysis of recombinant GmPDIL-3a and GmPDIL-3b Table S1 List of primers for ATG-mutagenesis of GmPDIL-3a and GmPDIL-3b This supplementary material can be found in the online version of...

Ngày tải lên: 18/02/2014, 11:20

... properties of SmSmad1 and SmSmad1B as described above However, in the presence of SmSmad4 and the active mutant form of TbRI, both SmSmad2 and SmSmad4 interacted with GCN5, indicating the formation of ... from S mansoni Fig Structure of the SmSmad1B gene, cDNA and protein (A) Schematic representations of the SmSmad1B gene, cDNA and protein and the amino acid sequence of SmSmad1B protein Five exons ... of SmSmad2 and SmSmad4 and resulted in the formation of a stable and functional Smad complex [6,7] The above results are consistent with the report that human GCN5 interacts with both TGFb- and...

Ngày tải lên: 18/02/2014, 16:20

... for cDNA single strand synthesis, 600 ng of RNA, 0.5 lg of oligo(dT) primer and 10 mm of dNTPs were incubated for at 65 °C Subsequently, lL of the first strand buffer X and lL of dithiothreitol ... III and P IV isoforms from the eluate P III ⁄ P IV (Fig 1C) Isoelectric focusing gave pI of 5.8, 5.7, 5.5 and 5.2 for the four isoforms respectively Secondary structure of the pulchellin isoforms ... mixture of other isoforms (B) Gel visualization of proteins eluted Lane 1, molecular weight markers Numbers on the left indicate the Mr values of the standards in thousands Lanes 2–9, lg of peak...

Ngày tải lên: 18/02/2014, 16:20

... Foundation of Raisiogroup (Raisio, Finland), the Finnish Funding Agency for Technology and Innovation (TEKES) and the Commission of the European Communities, specifically RTD program ‘Quality of Life and ... Da) The identity of this peptide was then confirmed by MALDI-TOF ⁄ TOF fragment ion analysis, where partial sequences of this peptide were obtained from the ladders of b-fragment and y-fragment ... Degree of inhibition of T reesei TYR2 as determined in the presence of 15 mM L-dopa, as analyzed by oxygen consumption and spectrophotometric assay oxidation of the substrate, presumably because of...

Ngày tải lên: 19/02/2014, 06:20

... stresses used for treatment of pea seedlings were cold (A and B), salinity (C and D), wounding (E and F), drought (G and H), SA (I and J), ABA (K and L) and calcium (N and O) Panel M is the control ... Figure 7A, D, I and L are diamidino-2phenylindole hydrochloride (DAPI) stained cells showing the nucleus, and Fig 7C, F, K and N are the merged images of A and B, D and E, I and J, and L and M, respectively ... ORF of 678 bp, a 5¢ UTR of 131 bp and a 3¢ UTR of 163 bp including 20 bp of poly(A) tail The PsCBL ORF encodes a protein of 225 amino acid residues with a calculated molecular mass of 25.9 kDa and...

Ngày tải lên: 19/02/2014, 07:20

... ENDGAPS, off; NOPGAPS, off; NOHGAPS, off Graphical output of the bootstrap figure was produced by the program TREEVIEW Isolation of RNA and RT-PCR analysis Total RNA was isolated from RTG-2 cells and ... initiation cycle of 15 at 95 °C, amplification cycles of 0.5 at 94 °C, 0.5 at 50 °C and 0.5 at 72 °C, and one termination cycle of at 72 °C, with 35 cycles in total for HSF1a and HSF1b and 30 for heat ... HSF1 isoforms form both homo- and heterotrimers, there are four potential assemblies of HSF1 trimer, namely, two homotrimers (a3 and b3) and two heterotrimers (a2b1 and a1b2) The existence of the...

Ngày tải lên: 19/02/2014, 12:20

... comparison of band absorbance Absence of a sharp and convoluted spectral band around 3600–3000 cm)1 in the spectra of both chitosan and LMWC indicated the absence of free -OH groups and the involvement ... weight of GlcNAc [24] Further characterization of chitosan and the depolymerization products Further characterization of chitosan and the depolymerization products was performed using a variety of ... (moles/L) · volume of titrant (mL)/mass of sample (g) and the level of deacetylation was calculated by comparison between the number of free amino groups (per unit weight of the sample) and the equivalent...

Ngày tải lên: 19/02/2014, 12:20

... and characterization of GAT from wild watermelon leaves, and discusses its function during drought ⁄ strong-light stress on the basis of its two unique enzymatic properties; thermotolerance and ... (DTT) and 20% (v ⁄ v) glycerol, and 20% saturation of (NH4)2SO4 The enzyme was eluted with a linear gradient of 30–0% saturation of (NH4)2SO4 in buffer A Active fractions were collected and applied ... Gv & Kwon YM (2001) cDNA cloning of two isoforms of ornithine carbamoyltransferase from Canavalia lineata leaves and the effect of site-directed mutagenesis of the carbamoyl phosphate binding...

Ngày tải lên: 20/02/2014, 03:20

... development of antigenomic therapies, such a reagent might also be useful in investigating the rate of turnover of mtDNA and in the derivation of mtDNA-free (q°) clones of the large number of cell ... potential, and addition of a TPP-moiety has been used to drive the selective uptake of a wide variety of Ó FEBS 2003 Fig Selective uptake of mitoDC-81 by mitochondria and subsequent alkylation of mtDNA ... the vicinity of mtDNA with the resultant binding and selective alkylation leading to a depletion of mtDNA in intact cells (Fig 1) Here we report the synthesis and characterization of a novel mitochondria-targeted...

Ngày tải lên: 20/02/2014, 11:20

... of 50 nmÆmin)1, band-width of 1.0 nm, and a resolution of 0.1 nm in the temperature range between 10 °C and 70 °C, after preincubation for 10 at each temperature Three runs were accumulated and ... of 0.6 lM The data were fitted to Michaelis–Menten curves Squares and dotted line: data and fitting with PHI/PHO ratio of 0.5 Triangles and continuous line: data and fitting with PHI/PHO ratio of ... Asterisks and broken line: data and fitting with PHI/PHO ratio of hypothesis and therefore a direct involvement of PHI in electron transfer This conclusion is consistent with the very low degree of identity...

Ngày tải lên: 21/02/2014, 00:20