proteus mirabilis escherichia coli and three normal bowel flora isolates eubacterium peptostreptococcus and bacteroides
Tài liệu Báo cáo khoa học: Ionic strength and magnesium affect the specificity of Escherichia coli and human 8-oxoguanine-DNA glycosylases pdf
Ngày tải lên :
18/02/2014, 18:20
... strength and Mg2+ on the activity and specificity of OGG1, we measured the apparent values of k2 and k3 under conditions of low salt (KPi only) and no Mg2+, low salt and 20 mm Mg2+, and high salt ... of Fpg and OGG1 The conditions inside a living cell differ from most buffer systems in which the activity and specificity of Fpg and OGG1 have been studied For example, in Escherichia coli the ... and A by Fpg and OGG1 depends on buffer composition, ionic strength, Mg2+ concentration and several other factors 3748 Results Effects of ionic strength and divalent cations on the activity and...
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Báo cáo khoa học: Theoretical study of lipid biosynthesis in wild-type Escherichia coli and in a protoplast-type L-form using elementary flux mode analysis potx
Ngày tải lên :
22/03/2014, 21:20
... by Escherichia coli and Corynebacterium glutamicum Metab Eng 8, 353–369 20 Fischer E & Sauer U (2003) A novel metabolic cycle catalyzes glucose oxidation and anaplerosis in hungry Escherichia coli ... und N-Formen Theoretical study of lipid biosynthesis in E coli 27 28 29 30 31 32 33 34 35 36 37 38 39 40 von Escherichia coli, Proteus mirabilis und Streptomyces hygroscopicus ⁄ vorgelegt von ... synthase from Escherichia coli Biochim Biophys Acta 1348, 192–200 Wyckoff TJ & Raetz CR (1999) The active site of Escherichia coli UDP-N-acetylglucosamine acyltransferase Chemical modification and site-directed...
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Báo cáo y học: " Multi-resistant Escherichia coli and mycotic aneurysm: two case reports" ppsx
Ngày tải lên :
11/08/2014, 17:21
... AmpC betalactamases among Escherichia coli and Klebsiella spp in the UK and Ireland J Antimicrob Chemother 2007, 59:102-105 Porkhurst GF, Decker JP: Bacterial aortitis and mycotic aneurysm of the ... study and drafting the manuscript AF summarised the two case studies and helped to draft the manuscript JC and SR, along with collaboration from NW and SL, assisted in identifying the strains and ... and raised inflammatory markers, with an ESR of 84 mm/hr and Creactive protein of 176 mg/l Chest X- ray revealed no abnormalities of clinical significance Cultures of blood and urine grew E coli...
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Expression analysis of y TMT AND FAD2 gene in escherichia coli and transgenic trichoderma reesei
Ngày tải lên :
22/05/2016, 11:28
... production in E coli Cells; A Separation of - and tocopherol product standards; B E coli BL21(DE3)/pET28a controls; C E coli BL21(DE3)/pET-PfTMT transformation The protein from E coli BL21 / pET28a ... HPLC analysis of -tocopherol production in E coli Cells; A Separation of and -tocopherol product standards; B E coli BL21(DE3)/pET30a controls; C E coli BL21(DE3)/pET-PfTMT transformation ………………………………………………… ... of - and tocopherol were observed at 4.285 and 2.451 for UV detection (Fig 2.12A, 2.13A) In HPLC analysis of - and -tocopherol standards, the peaks likewise eluted at these times, and showed...
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Tài liệu Báo cáo " synthesis, cloning and expression in escherichia coli of a gene coding for Mcoti-ii " ppt
Ngày tải lên :
12/02/2014, 10:20
... NdeI and XhoI restriction enzymes (fig 6) and ligated with pTYB12 vector had been digested by the same restriction enzymes, then transformated into E .coli BL21 (DE3) The resulting recombinant E .coli ... the 58 kD protein band was found in induced sample as the major band, acounted for 80% of total protein (lane 1,3,5 and 6) 58 kD Fig 10 SDS PAGE pattern of PI-17 proteins Mc Standard molecular ... Michal Lebl and Richard A Houghten (Editors), American Peptide Society, 2001, pp 387-388 Bozenna Rempola, T-Wilusz, W Markiewicz and M.Fkus, Synthesis, cloning and expression in E .coli of the...
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Tài liệu Báo cáo khoa học: Basis of recognition between the NarJ chaperone and the N-terminus of the NarG subunit from Escherichia coli nitrate reductase pdf
Ngày tải lên :
16/02/2014, 14:20
... References Magalon A & Mendel RR (2008) Biosynthesis and insertion of the molybdenum cofactor In EcoSal Escherichia coli and Salmonella: cellular and molecular biology (Bock A, Curtiss R III, Kaper ... ± 0.2 and a binding constant (Kd) of 3.4 ± · 10)9 m and a second with a stoichiometry of 0.7 ± 0.1 and a Kd of 3.3 ± · 10)7 m (Fig 5A) Identical results were obtained using NarJ or NarJT and both ... resonances and the appearance of new resonances upon complex formation indicated a slow exchange on the NMR timescale between the free and the bound forms for NarJT These results and the higher...
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Tài liệu Báo cáo khoa học: Mechanism of dihydroneopterin aldolase NMR, equilibrium and transient kinetic studies of the Staphylococcus aureus and Escherichia coli enzymes docx
Ngày tải lên :
19/02/2014, 00:20
... using both DHNP and DHMP as the substrates and the reverse reaction (the formation of DHNP and DHMP) with HP and GA For the forward reaction, three concentrations each for DHNP and DHMP were used ... rate constants are similar between NP and MP and 2248 between HP and HPO in accordance with the similar shapes and sizes between NP and MP and between HP and HPO The different Kd values are proportional ... via the deprotonation and re-protonation of C2¢ and report a comprehensive equilibrium and kinetic study of SaDHNA and EcDHNA, which represent DHNAs from Gram-positive and Gram-negative bacteria,...
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Tài liệu Báo cáo khoa học: Critical roles of Asp40 at the haem proximal side of haem-regulated phosphodiesterase from Escherichia coli in redox potential, auto-oxidation and catalytic control doc
Ngày tải lên :
19/02/2014, 16:20
... 2004 oxygen and carbon monoxide to a heme-regulated phosphodiesterase from Escherichia coli: kinetics and infrared spectra of the full-length wild-type enzyme, isolated PAS domain and Met95 mutants ... of a direct oxygen sensor heme proteins from Escherichia coli: effects of the heme redox states and mutations at the heme-binding site on catalysis and structure J Biol Chem 277, 23821–23827 Yoshimura, ... between heme incorporation, tetramer formation, and catalysis of a heme-regulated phosphodiesterase from Escherichia coli A study of deletion and site-directed mutants J Biol Chem 278, 53105–53111...
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Tài liệu Báo cáo khoa học: The sensor protein KdpD inserts into the Escherichia coli membrane independent of the Sec translocase and YidC doc
Ngày tải lên :
20/02/2014, 11:20
... K+-translocating Kdp system of Escherichia coli J Biol Chem 271, 25027–25034 Pogliano, K.J & Beckwith, J (1994) Genetic and molecular characterization of the Escherichia coli secD operon and its products J ... of Escherichia coli requires the signal recognition particle, SecA and SecYEG J Biol Chem 277, 5715–5718 Andersson, H & von Heijne, G (1993) Sec dependent and sec independent assembly of E coli ... concentration) and 2.5% Triton X-100 (lanes and 6) All samples were precipitated with 20% trichloroacetic acid, immunoprecipitated with antiserum to OmpA (upper panel) and KdpD (lower panel) and analysed...
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Tài liệu Báo cáo khoa học: Steady-state and time-resolved fluorescence studies of conformational changes induced by cyclic AMP and DNA binding to cyclic AMP receptor protein from Escherichia coli ppt
Ngày tải lên :
20/02/2014, 23:20
... 1ị where SD and SRF are the respective areas under the emission spectra of the donor and a reference compound, and ARF and AD are the respective absorbances of the reference compound and donor ... 10PỵDAị=2 2ị where F and Fcor are uorescence intensity before and after the correction, and P and DA denote the initial sample absorbance at the excitation wavelength and the change in absorbance ... receptor protein from Escherichia coli with cAMP and DNA studied by dynamic light scattering and timeresolved uorescence anisotropy methods J Protein Chem 20, 601610 31 Sứgaard-Andersen, L., Mironov,...
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Tài liệu Báo cáo khoa học: Dimer asymmetry and the catalytic cycle of alkaline phosphatase from Escherichia coli doc
Ngày tải lên :
21/02/2014, 00:20
... obtained using the curve-fitting procedure and describing the affinity of the subunits for the substrate (KS1 and KS2) and for Pi (KI1 and KI2), presented in Table and Table 2, respectively, are independent ... containing either no Mg2+ (+), or 2.1 · 10)6 M, (s); 2.1 · 10)5 M, (d) and 2.1 · 10)3 M ( · ) Mg2+ between KS1 and KS2, and KI1 and KI2, respectively) Also, parameter b is not significantly dependent ... Acta 186, 175–187 Anderson, R.A & Vallee, B.L (1975) Cobalt (III), a probe of metal binding sites of Escherichia coli alkaline phosphatase Proc Natl Acad Sci USA 72, 394–397 Anderson, R.A., Bosron,...
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Tài liệu Báo cáo Y học: Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenol pyruvate carboxykinase determined by fluorescence spectroscopy pdf
Ngày tải lên :
21/02/2014, 01:21
... several ligands and on the role of Mn2+ and Mg2+ on their binding The comparison between acrylamide quenching studies and modeled structures of free and substrate bound P-pyridoxylE coli PEP carboxykinase ... uorescence changes upon ligand binding here described show that the P-pyridoxyl group is a useful probe to monitor ligand binding and ligand-induced conformational changes in E coli PEP carboxykinase ... carboxykinases from Escherichia coli and Saccharomyces cerevisiae FEBS Lett 360, 207210 Metzler, D.E & Snell, E.E (1955) Spectra and ionization constants of the vitamin B6 group and related 3-hydroxypyridine...
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Tài liệu Báo cáo Y học: The glucose-specific carrier of the Escherichia coli phosphotransferase system Synthesis of selective inhibitors and inactivation studies pptx
Ngày tải lên :
21/02/2014, 01:21
... encodes the three subunits of the Escherichia coli mannose transporter under the control of the Ptac promoter [20] Membranes containing EIIGlc and EIIMan, and purified EIIGlc, EI, HPr, IIAGlc and IIABMan ... k2 ¼ 32 min)1, KI2 ¼ 700 lM KS1 and KS2 are the dissociation constants of E1 and E2 for Glc, KI1 and KI2 the dissociation constants for the inhibitor, and k1 and k2 are the turnover numbers DYNAFIT ... isothiocyanate, epoxide and a,b-unsaturated ester functions at positions C-1 and C-6 were synthesized and characterized as pseudosubstrates, reversible and irreversible inhibitors of EIIGlc and EIIMan The...
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Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf
Ngày tải lên :
21/02/2014, 01:21
... unfused and fused proteins in Escherichia coli Gene 69, 301–315 45 Hassan, H.M & Fridovich, I (1978) Superoxide radical and the oxygen enhancement of the toxicity of paraquat in Escherichia coli ... for analysis of SOD activity and were calculated to give unit of SOD activity in the standard SOD assay conditions used and all values were normalized to this At least three independent measurements ... within 2.1 A of the metal ion) Azide binding residues include the three His metal ligands (H26, H81 and H171) and gateway residues H30 and H34 A further gateway residue, H31, is involved in FeSOD...
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Tài liệu Báo cáo Y học: Insights into the reaction mechanism of Escherichia coli agmatinase by site-directed mutagenesis and molecular modelling ppt
Ngày tải lên :
21/02/2014, 01:21
... sequence alignment of B caldovelox arginase (1cev), E coli agmatinase (AUH) and rat liver arginase (1rla) H, S, T and stands for a-helix, strand, turn and 310-helix Conserved residues are marked by ... ACCTATGCG-3¢) and the 3¢ complementary primer 5¢-AT TAATGGCATGCTTTACCCGT-3¢ Using the PCR products of agmatinase with the GfiA and CfiT substitutions in the coding and noncoding strands, respectively, and ... wild-type and D153N mutant enzymes (5.5 lM) were measured in the range 200–250 nm, with a bandwidth of nm and a scan speed of 50 nmÆmin)1 The buffer solution contained mM Tris/HCl (pH 7.5) and mM...
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Tài liệu Báo cáo Y học: Structure of the O-polysaccharide and classification of Proteus mirabilis strain G1 in Proteus serogroup O3 potx
Ngày tải lên :
21/02/2014, 15:20
... serum recognized slow migrating bands of three LPS (without R14) and fast migrating bands of P mirabilis G1, O28, and R14 LPS (Fig 5A) These bands correspond to high- and low-molecular-mass LPS species ... anti-(O-polysaccharide) serum Control 256 000 P mirabilis G1 2000 P mirabilis S1959
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Tài liệu Báo cáo Y học: Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx
Ngày tải lên :
22/02/2014, 07:20
... expression and rapid purification of Escherichia coli GroEL and GroES chaperonins Prot Exp Purif 11, 47–52 34 Nobelmann, B & Lengeler, J.W (1996) Molecular analysis of the gat genes from Escherichia coli ... Blankenhorn, D., Welty, D., Zinser, E & Slonczewski, J.L (1994) Acid and base resistance in Escherichia coli and Shigella flexneri: role of rpoS and growth pH J Bacteriol 176, 1729–1737 Jenkins, D.E., Auger, ... on Escherichia coli protein synthesis and survival during growth and prolonged starvation Mol Microbiol 11, 1029–1043 Farewell, A., Diez, A.A., DiRusso, C.C & Nystrom, T (1996) ¨ Role of the Escherichia...
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Báo cáo khoa học: Functional dissection of Escherichia coli phosphotransacetylase structural domains and analysis of key compounds involved in activity regulation potx
Ngày tải lên :
06/03/2014, 11:20
... manufacturer’s recommendations Protein expression and purification E coli Pta and Pta-F1, Pta-F2 and Pta-F3 were produced in E coli K-12 AG1, E coli BL21(DE3) or E coli M15 containing the corresponding expression ... coli K-12 AG1 for pCA24N–Pta; E coli BL21(DE3) for pET28–F1 and pET28–F2; and E coli M15 for pQE30–F3 Construction of the E coli acs pta deletion strain The E coli acs pta deletion strain (FB22) ... Pta and the three truncated Ptas in both the forward and reverse reactions (Fig 5A) NADH and ATP substantially inhibited the activity of E coli Pta in both directions (Fig 5A) On the other hand,...
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Báo cáo khoa học: High resolution structure and catalysis of O-acetylserine sulfhydrylase isozyme B from Escherichia coli pot
Ngày tải lên :
07/03/2014, 05:20
... folds in Fig Deviations occurred at the N- and C-termini and at the four surface loops at positions 21, 60, 190 and 271, far away from the active center and also from the dimer interface These differences ... close to PLP and not mobile (Fig 5), although it participates in the induced fit (Fig 4) The mobility distributions of CysM(K268A), wildtype CysM and CysM(salmo), and those of subunits B and D of ... bottom are from dimers A–B and C–D of CysM(RKE) [6] which, however, were split into an average of the closely related subunits B and D and the equally well-related subunits A and C observed asymmetry...
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Báo cáo khoa học: Escherichia coli cyclopropane fatty acid synthase Mechanistic and site-directed mutagenetic studies potx
Ngày tải lên :
07/03/2014, 16:20
... in the E coli enzyme, all of the eight cysteines are free and that they can be classied into three classes: three fast reacting cysteines, two cysteines reacting at a moderate rate, and three cysteines ... Salmonella typhimurium and Escherichia coli: structure and metabolism J Bacteriol 95, 833 843 Dittmer, J.C & Wells, M.A (1969) Quantitative and qualiative analysis of lipids and lipid components ... modication and site-directed mutagenesis It was found that the cysteines are not essential for catalysis E coli strains JM109 and BL21(DE3) were from Promega (Madison, WI, USA), and E coli K12...
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