proteomic analysis of protein extracted from tissue cells

... of phosphorylated JNK1&2 (-fold of control) = absorbance of hypoxia-treated cells/ absorbance of control cells RT-PCR analysis Total RNA was extracted from JAR cells using RNeasy mini kits (QIAGEN, ... ↑ ↑ ↓ + + + + + ↓ ↑ ↑ ↑ ↑ + + + + Protein expression: ↑ means increased expression of protein ↓ means decreased expression of protein → means no changes of protein expression means negative expression ... decreases of protein expression, respectively Both decreases and increases in the expression of proapoptotic and antiapoptotic proteins were detected PowerBlot analysis showed altered expression of...

Ngày tải lên: 31/10/2012, 15:12

... mLÆmol)1 which might be the DV minimum of the protein (or of the system: protein, solvent and sucrose) in the presence of sucrose From this value, the P1/2 of the protein was estimated to be % 400 ... degree of unfolding of the protein (a) was the same as reported previously and was calculated either from the centre of spectral mass (CSM) for fluorescence measurement or from the amplitude of ... Stabilization of 33-kDa protein of spinach PS II against pressure unfolding (Eur J Biochem 270) 1657 Fig The effect of sucrose (m), glycerol (j) and salt (d) on the standard volume change of the protein...

Ngày tải lên: 31/03/2014, 01:20

... cause of death was obtained from the Swedish Cause of Death Registry up until 31 Dec 2009 Tissue microarray construction Paraffin-embedded tumour specimens were collected from the archives of the ... biomarker discovery based on tissue- specific protein profiles in normal and cancer tissues Mol Cell Proteomics 2008, 7:825-844 13 Ponten F, Jirstrom K, Uhlen M: The Human Protein Atlas–a tool for ... level of RBM3, a member of the glycine-rich RNA-binding protein family, in human cells in response to cold stress Biochem Biophys Res Commun 1997, 236:804-807 10 Lleonart ME: A new generation of...

Ngày tải lên: 20/06/2014, 04:20

... The purpose of this study was to demonstrate a membrane proteomic analysis of PDAC cells and to identify differentially expressed membrane proteins between primary and metastatic PDAC cells, which ... identification of Liu et al Journal of Biomedical Science 2010, 17:74 http://www.jbiomedsci.com/content/17/1/74 Figure Analysis and identification of membrane proteins in AsPC-1 and BxPC-3 cells using a proteomics ... assigned as membrane proteins in BxPC-3 cells Table lists the “integral to membrane” proteins found in AsPC-1 and BxPC-3 cells Besides the membrane proteins, the proteomic analysis also identified...

Ngày tải lên: 10/08/2014, 05:21

... iTRAQ data analysis 68 4.2.1 Effects of hypoxia on protein profiles in the 68 presence/absence of p53 4.2.2 Gene ontology and protein- protein interaction 73 analysis using Ingenuity Pathway Analysis ... and p53 by analyzing their protein profiles At 95% C.I., a total of 217 proteins were identified in our iTRAQ experiments and of which, the expression levels of 54 proteins were found significantly ... display of a merged top protein- protein interaction 78 network generated by IPA tool from the 54 iTRAQ target proteins with at least 30% abundance change in protein expression level 4.5 A protein...

Ngày tải lên: 16/09/2015, 15:43

... Levels of the RAN binding protein were reduced in a-syn cells with respect to b-gal cells and RuvB-like is upregulated in a-syn cells in the absence of dopamine CRMP4, a member of a family of neuron-enriched ... Journal compilation ª 2010 FEBS T Alberio et al Proteomics of a PD model Fig A representative silver-stained 2-DE gel of proteins extracted from b-gal cells treated with catalase (cat) Qualitative ... expression of a-synuclein in b-gal control cells but not in a-syn cells that already overexpress it as a consequence of transfection (Fig 1) Proteomics analysis reveals quantitative changes in 23 proteins...

Ngày tải lên: 15/02/2014, 01:20

... using PAUP software [23] IFN-c was used as an outgroup in this analysis Expression of the IL-10 gene Analysis of IL-10 gene expression in healthy tissues by RT–PCR Total RNA extracted from the cell ... distant from IL-20 and IL24, as recently determined from analysis of the spotted green pufferfish genome, imply that the carp sequence is IL-10 The hydropathy analysis also shows similarity of the ... following protocol: lL of cDNA was mixed with lL of dNTPs (10 lM of each dNTP), 10 · Gene Taq Universal buffer, 0.5 lL of Taq polymerase (5 UÆmL)1; Nippon Gene, Tokyo, Japan), lL of each gene specific...

Ngày tải lên: 20/02/2014, 02:21

... content of proteins from circular dichroism spectrum Comput Appl Biosci 9, 197– 199 Ó FEBS 2002 36 Laemmli, U.K (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage ... These types of dioxygenases are encoded by genes of moderate to high sequence identity (from 19% to 75%), which might catalyse very diverse reactions irrespective of their relative degree of sequence ... evolved from a common ancestral gene, and the essence of most of the conserved amino acids has been further substantiated by site-directed mutagenesis of FHT [7,9] and by documentation of ligand...

Ngày tải lên: 21/02/2014, 03:20

... calculated from the amino acid sequence, indicating that the native form of avoredoxin is a dimer Amino acid sequence analysis of avoredoxin The N-terminal amino acid sequence of avoredoxin from DvMF ... Figure shows the results of linear regression analysis of the logarithms for the redox ratio of the mediator versus that of recombinant avoredoxin The redox potential of oxidized avoredoxin reduced ... both of which confer reduction of a avin by receiving electrons from NADH or NADPH; however, these activities of avoredoxin were much lower than those of A fulgidus FeR The FeR activity of A...

Ngày tải lên: 07/03/2014, 02:20

... indicative of a number of intersheet hydrogen bonds in both of the molecules The exchanging properties of the amide protons of SGCI[L30R, K31M] are very similar to that of SGCI Both the amide proton of ... differing conformation of their binding loops The corresponding NOE pattern of SGTI is different from that of SGCI as well as from that recorded for SGCI[L30R, K31M] The number of observed NOEs for ... raise the question of the universal nature of serine proteinase structure among animals and particularly, mechanism of inhibition by natural protein inhibitors In the light of the solution structures...

Ngày tải lên: 08/03/2014, 10:20

... Guideline for the Conduct of Food Safety Assessment of Foods Derived from Recombinant-DNA Plants (CAC/GL 45-2003), the Guideline for the Conduct of Food Safety Assessment of Foods Produced using ... Conduct of Food Safety Assessment of Foods Derived from Recombinant-DNA Animals (CAC/GL 68-2008) See footnote Reference is made to the Compilation of Codex Texts Relevant to Labelling of Foods ... information relevant to risk analysis 30 Recognizing the rapid pace of development in the field of biotechnology, the approach to safety assessments of foods derived from modern biotechnology should...

Ngày tải lên: 13/03/2014, 21:56

... Conformation of meningococcal Opa proteins (Eur J Biochem 269) 5219 Fig Semi-native PAGE analysis of in vitro folding of OpaB128 (A) and OpaJ129 (B) (A) Semi-native-PAGE analysis of in vitro folding of ... fragment of opaB128 and opaJ129 encoding the mature domain of the protein downstream of the promoter and the signal sequence-encoding part of the phosphate-limitation inducible phoE gene of E coli ... single individual Opa protein without significant contamination from different Opa proteins expressed from other loci However, for detailed structure–function analysis pure protein is needed and...

Ngày tải lên: 17/03/2014, 10:20

... distribution The presence of Gly and DL-Ala in the particles is Organic analysis of microparticles from Itokawa 67 68 H Naraoka et al Fig Mass spectra of positive ions in ToF-SIMS analysis a) Hayabusa ... Hayabusa particles extract, and b) blank extract Organic analysis of microparticles from Itokawa 69 Fig Mass spectra of negative ions in ToF-SIMS analysis a) Hayabusa particles extract, and b) blank ... spectra of ToF-SIMS are shown in Figs and The predominant peaks of positive ions are at m/z = 27 and 28 (Fig 5), which are attributable to the aluminum from foil used for baking and silicon from...

Ngày tải lên: 28/03/2014, 20:20

... H-5, H-7 of Kdoa H-3 of HepI; H-1 of HepIII H-1, H-2 of HepII; H-1 of GlcII; H-1 of GlcII* H-4, H-6 of HepI H-4, H-6 of HepI H-4, H-6 of HepI H-1, H-2 of HepIII H-1, H-2 of HepIII H-4 of GlcII*d,e ... O-6 of the Ó FEBS 2002 Structural analysis of LPS from NTHi strain 1003 (Eur J Biochem 269) 815 Table Proton NOE data for O-deacylated LPS and oligosaccharide preparation OS-1 derived from LPS of ... Hex1 and Hex4 glycoforms were indicated Ó FEBS 2002 Structural analysis of LPS from NTHi strain 1003 (Eur J Biochem 269) 811 Fig Negative ion ESI-MS spectrum of O-deacylated LPS from NTHi strain...

Ngày tải lên: 31/03/2014, 21:21

... calculation of response functions for the subsamples (calibration), which are randomly extracted with replacement from the initial data set The size of each subsample is the same as that of the initial ... examinations of the radial growth of pine trees (Pinus densiflora S et Z) on Dendrochronological Mt Namsan and the potential effects of current level of air pollutants to the growth of the trees ... of dating and measurement accuracy was performed by correlating overlapping 50-year segments of all measured series using the program COFECHA [10] This program helps in identifying segments of...

Ngày tải lên: 09/08/2014, 04:20

... of a-amylase are species-specific [8] The present report gives an analysis of the phenotypic variability of genotypes homozygous for the Amy and Amy allele of the Amy locus at the level s F of ... 0.05), as well as between the groups of lines of both genotypes within ± SE of the mean (U 24.00, P < 0.05) The analysis of intergroup differences in the number of active regions for the six groups ... both the variability of the structural Amy locus and the variability of tissue- specific expression [9] The latter type of variability is represented by the number and position of the amylase activity...

Ngày tải lên: 09/08/2014, 18:21

... pool Each isofemale line derived from this pool carries a sample of alleles, ranging from one extreme to the other (from p = 1.0 toq = 1.0) The relative frequency of each type of isofemale line, ... matrix of pair-wise distance values B UPGMA cluster analysis ’ As one way of summarizing differences between all pairs of isofemale lines, hierarchical cluster analyses were performed on a matrix of ... the polygenic loci of interest, the genetic makeup of isofemale strains will differ as a function of the gene frequencies in the population and the probabilities of each type of mating a In this...

Ngày tải lên: 09/08/2014, 22:22

... Use of the Advanced Photon Source is supported by the U S Department of Energy, Office of Sciences, Office of Basic Energy Sciences, under Contract No DEAC02-06CH11357 Author details Institute of ... between 2D and 3D analysis The figure shows four x-ray micrographs from 3D pellet specimens of EMT cells These projection images are from large angular sets (Additional file 8) from which tomographically ... size (nm) B S3 S2 Figure 11 Quantitative analysis of the uptake of EMT cells by transmission x-ray microimages Transmission x-ray uptake microanalysis by EMT cells after co-culturing with 500 μM...

Ngày tải lên: 11/08/2014, 00:23

... involved in the activation of B cells, the promotion of growth and the survival of T cells, the inhibition of macrophage and the activation and suppression of Th-1 cells Recent studies have showed ... different from the control value Figure Effect of various concentrations of flavonoùds extracted from A herba alba on IL-12 production in PBMC (5 x106 cells/ mL) of patients with ABD Presence of cytokines ... significantly different from the control value C Concentration of nitric oxide in the supernatants of culture of PBMC from patients with Adamantiades-Behỗets disease and healthy controls Cells were treated...

Ngày tải lên: 11/08/2014, 03:20