proteomic analysis of body fluids

... to the formula: Changes in the level of phosphorylated JNK1&2 (-fold of control) = absorbance of hypoxia-treated cells/absorbance of control cells RT-PCR analysis Total RNA was extracted from ... transfection of Bag-1 Morpho/AS to JAR cells did not alter the expression of PTEN after the exposure to hypoxia, which would mean that the expression of PTEN, and also that of Bcl-X, was independent of ... expression profiles of proteins involved in the mitochondrial (bcl-2, bax, and bcl-x, etc.) and stress reaction-related pathways of apoptosis (Fig 2) Table Altered protein expression of JAR cells...

Ngày tải lên: 31/10/2012, 15:12

... specifically linked to one of the major pathogenetic mechanisms of PD [5] Worthy of note is the complete disappearance of the VDAC-2 upon dopamine treatment This porin of the outer mitochondrial ... familial forms of PD [6] However, a-synuclein deposition into LB is a general hallmark of the PD state, suggesting that the accumulation of a-synuclein might cause selective degeneration of dopaminergic ... increased the expression of a-synuclein in b-gal control cells but not in a-syn cells that already overexpress it as a consequence of transfection (Fig 1) Proteomics analysis reveals quantitative...

Ngày tải lên: 15/02/2014, 01:20

... identification of Liu et al Journal of Biomedical Science 2010, 17:74 http://www.jbiomedsci.com/content/17/1/74 Figure Analysis and identification of membrane proteins in AsPC-1 and BxPC-3 cells using a proteomics ... molecules are predictors of metastatic risk and also provide a basis for the development of anti-metastatic therapy Our proteomic analysis has revealed a large number of differentially expressed ... number of peptides matched This may reflect the differential level of a Liu et al Journal of Biomedical Science 2010, 17:74 http://www.jbiomedsci.com/content/17/1/74 Figure Western blot analysis of...

Ngày tải lên: 10/08/2014, 05:21

... Figure GoMiner analysis of the molecular function of identified proteins GoMiner analysis of the molecular function of identified proteins High-confidence and high-coverage analysis of the seminal ... tasks of the spermatozoa [31] The potential use of the proteomic data set for biomarker discovery Identification of disease biomarkers is an overarching aim of large proteomics studies of bodily fluids ... followed by MS analysis A total of 139 proteins were reported, but many of them were identified only with a single peptide and with low identification scores Many of the body fluid proteomics projects...

Ngày tải lên: 14/08/2014, 16:21

... proportion of both Army and AFW samples Two-thirds of the officers in the Army sample and 84% of the AFW officers' samples were nurses Some 35% of the enlisted personnel in the Army and 52% of the ... number of measurements of body size variIndeed, ability (Air Force women 138, and Army women, 142) a major impetus for the conduct of these surveys was the desire to develop an adequate base of body ... contour of the body THE SUMMARY STATISTICS ARMY NO OF SUBJECTS MEAN 'ST DEVe GOEF OF VARe SYMMETRY KURTOSIS 1331 9.95 INCHES e62 INCHES 8.2% ,20 3.01 AFW NO OF SUBJECTS MEAN ST OEV COEF OF VAR*...

Ngày tải lên: 15/08/2015, 01:52

... Scheme of a MALDI-TOF instrument (d) Scheme of a MALDI-TOF/TOF instrument (Mocellin et al., 2004) 22 1.1.4.2 Types of MS The names of the various instruments are derived from the name of their ... 1.1.4.2 Types of Mass spectrometry 23 1.1.5 Array-based proteomics 24 1.1.6 Applications of proteomics 26 iii 1.1.6.1 Expression proteomics 26 1.1.6.2 Functional proteomics 27 1.1.7 Proteomics in ... Graphical representation of 24 identified proteins 92 17 A representative MALDI TOF-TOF MS spectrum 93 18 Western blot analysis of BAL fluid in acute asthma model 95 19 RT-PCR analysis in acute asthma...

Ngày tải lên: 14/09/2015, 22:03

... iTRAQ data analysis 68 4.2.1 Effects of hypoxia on protein profiles in the 68 presence/absence of p53 4.2.2 Gene ontology and protein-protein interaction 73 analysis using Ingenuity Pathway Analysis ... wrote “Proteomics: the analysis of complete complements of proteins Proteomics includes not only the identification and quantification of proteins, but also the determination of their localization, ... the domains of p53 36 1.8 Proposed model showing different levels of HIF-1-p53 interactions 42 in the presence of hypoxia and anoxia 1.9 Different p53 isoforms and their mechanisms of production...

Ngày tải lên: 16/09/2015, 15:43

... activation of macrophages 1.2.1 IFN gamma 1.2.2 Effects of IFN-γ on macrophages 1.3 Proteomics studies of macrophages 1.3.1 Strategies of proteomics analysis ... batches (BMM1,2) One batches (BMM2) Two batches (BMM5,6) Transcriptomics analysis Proteomic analysis Proteomics analysis Proteomics analysis (mRNA expression array) (2DE & MALDI-MS/MS) (2D-DIGE & LC-MS/MS) ... on 2-DE proteomic reference map Distribution of identified proteins Number of containing spots Proteins 99 23 7 10 17 19 One of the most important criteria for quantitative proteomic analysis...

Ngày tải lên: 12/05/2016, 21:57

... of Saccharomyces cerevisiae proteomic analysis The importance of proteomic investigations in Saccharomyces cerevisiae The proteomic analysis of S cerevisiae is the next step in the analysis of ... ratios of the peptides In brief, there are many types of mass spectrometers applied for proteomic analysis, including Quadrupole MS, Time of flight (TOF) MS, and Quadruplole ion trap, MALDI-TOF-TOF, ... identification and quantitation of S cerevisiae proteins in proteomics experiments 43 2.2.4 Proteomics in the study of S cerevisiae networks 57 2.2.5 Proteomic analysis of S cerevisiae protein...

Ngày tải lên: 20/05/2016, 21:33

... Thesis Table of contents Page Acknowledgements Table of contents Part I Introduction Background information Aims of the study Objects of the study Methods of the study 5 Design of the study Part ... Investigation Chapter Idioms 1.1 Definitions of idioms 1.1.1 Points of view of English authors on idioms 1.1.2 Points of view of English authors on idioms 1.2 History of studying idioms 1.2.1 In England ... referring to body parts The third aim is that through this study, we try to give some types of exercises to improve the ability of using idioms referring to body parts of the learners Objects of the...

Ngày tải lên: 12/12/2013, 00:03

Ngày tải lên: 29/01/2014, 00:23

... comprehensive proteomics analysis of 14-3-3-binding proteins under physiological conditions as compared with apoptosis stimulation, with the aim of increasing our knowledge of the role of 14-3-3 ... identification of genuine 14-3-3 ligands Detailed analysis of the 14-3-3-asociated proteins found showed that 46 of them were exclusively present in one of the conditions analyzed and 15 of them were ... aliquot of the supernatant (1.5 mg of protein) was incubated with lg of antibody against VASP, antibody against B23 and antibody against TSC2 coupled to 20 lL of protein G–Sepharose for h at °C...

Ngày tải lên: 06/03/2014, 22:21

Ngày tải lên: 16/03/2014, 16:20

... described previously [45] Cut-off values were chosen on the basis of a measure of heterogeneity An optimal cut-off value was identified An intensity score of ‡ with at least 50% of malignant cells showing ... play an important role in the progression and metastasis of CRC In summary, the techniques of proteomic analysis provide a dramatic means of screening for genesis- and metastasis-associated proteins ... development and progression of CRC Further functional and clinical analysis of the proteins is necessary to elucidate their precise role in the process of CRC and the formation of metastases Experimental...

Ngày tải lên: 23/03/2014, 03:20

... number of ways: a) use of a different body lexeme b) use of meronyms vs holonyms c) use of a different verb d) use of lexically different words e) use of a compound vs two lexemes a) use of different ... patterns of idioms 21 1.8 Semantic aspects of English body idioms 25 1.9 Method of idiom analysis 28 Practical part 29 2.1 Typology of idom analysis ... internal organs of the human body: at the top of one’s lungs, to have the nerve, from the bottom of the heart, hard to stomach • Sensonymic lexis that denotes sense organs of the human body: to be...

Ngày tải lên: 07/04/2014, 02:22

... Communications and Networking Patient Patient Body sensor Body sensor Patient Body sensor Care unit BAN coordinator Patient Patient d < 8m Body sensor Body sensor Figure 1: A star-based BSN in a ... number of sensors is limited and an external mobile device, such as PDA or cell phone, acts as a BAN coordinator However, the promising accomplishment of the DQBAN protocol in terms of quality of ... here of fixed length (i.e., independent of the number of sensors in the BSN) and contains two new fields for specific energy-saving purposes, the modulation and coding scheme (MCS) and length of the...

Ngày tải lên: 21/06/2014, 11:20

... cuticle of most of the body wall of the larva is soft and pliable During the intermolt period the soft cuticle grows in thickness, but also increases in surface area through intercalary insertion of ... combined effects of cell multiplication and cell enlargement The mechanism of body size determination in Manduca The initial mass of each larval instar is a constant multiple of that of the previous ... predictions of the effect of growth rate on body size (a) Predicted effect of variation in growth rate on peak size; (b) predicted effect of variation in growth rate on peak size in the presence of a...

Ngày tải lên: 06/08/2014, 18:21

... time of local control was defined as the duration from the beginning date of SBRT to the date of local recurrence The time of survival was duration from the beginning date of SBRT to the date of ... design of the study and draft the manuscript; HJT and CLL worked on analysis of data and helped collection of data FX contribute to the conception of this study and the final approval of the ... for tumors of different size Based on the analysis of prognostic factors of overall survival rate in this study, clinical stage and chemotherapy were significant factors in multivariate analysis...

Ngày tải lên: 09/08/2014, 09:20

... generations of the three replicates of those lines selected divergently for high and low proportion of body fat, and in particular on changes in the variances of fat proportion during the course of selection ... directions of selection fitted as genetic groups was also fitted, except when only one direction of selection was included Method III comprised analysis of blocks, each of three generations of phenotypic ... objective of the lines was to change the proportion of body fat, but without greatly changing lean mass [9, 14, 28] Selection was practised on the ratio of the gonadal fat pad weight to total body...

Ngày tải lên: 09/08/2014, 18:21

... effects of inflammation important to understand, but also whether bone changes accompany early changes in cartilages as suggested above Analyses of data for ratios between biomarkers, such as those of ... joints The study population was very small, asking too much of statistical analyses, and often only SFs were examined [1] Studies were made of early events long before any degenerative structural ... 46:420-427 doi:10.1186/ar3276 Cite this article as: Poole AR: What measurements of molecular biomarkers in different body fluids really tell us? Arthritis Research & Therapy 2011, 13:110 ...

Ngày tải lên: 12/08/2014, 15:22