proteolytic activation of notch signaling roles for ligand endocytosis and mechanotransduction

báo cáo hóa học: " Signaling pathways mediating a selective induction of nitric oxide synthase II by tumor necrosis factor alpha in nerve growth factor-responsive cells" pptx

Ngày tải lên : 19/06/2014, 22:20

... expression of both TrkA and p75NTR [71,72] as well as the convergence of TrkA and p75NTR-mediated signaling impinging upon NF-κB [73] Page 10 of 13 (page number not for citation purposes) Journal of ... independent signaling of both types of NGF receptors [79] or recruitment of intracellular signalling elements uniquely driven by the simultaneous activation of both NGF receptors' signaling Thus, ... design and coordination and provided the expertise for RTPCR and withdrawal experiments HUS sub-cloned the PC12p75NTR(-) cells and participated in study design and result interpretation of experiments...

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Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx

Ngày tải lên : 14/02/2014, 19:20

... proteins of these subregions (Fig S1C) and 1654 Fig Of all members of the KIND family of proteins, only KIND2 binds to MAP2 Pull-down assay (PD) of the endogenous MAP2 from cerebellar lysates of P21 ... core sequence for v-KIND binding To evaluate the v-KIND KIND2-binding speciﬁcity of residues 702–744 of MAP2, we performed a pulldown assay of combinations of EGFP-fused KIND1 or KIND2 of v-KIND ... Sigma-Aldrich) and HA (catalog number: 1867423; Roche Diagnostics, Basel, Switzerland) were used at dilutions of : 1000 for immunoblotting, : 200 for immunocytochemistry and lgÆmL)1 for coimmunoprecipitation...

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Báo cáo khoa học: Liver receptor homolog-1 localization in the nuclear body is regulated by sumoylation and cAMP signaling in rat granulosa cells docx

Ngày tải lên : 23/03/2014, 06:20

... the effect of cAMP signaling on SUMO pathway is complex and cell type dependent Notwithstanding, the up regulation of E1, the down regulation of E2 or E3 and the increased expression of SENP2 lead ... reactions were performed by incubating lL of in vitro 35S-labeled product with lg of Aos1 ⁄ Uba2, 150 ng of UBC9 and lg of SUMO-1 in sumoylation buffer (20 mm Hepes, pH 7.5, mm MgCl2 and mm ATP) SAE1 ... 5¢-GGGAAATCGTGCGTGAC-3¢ and antisense, 5¢-CAAGAAGGAAGGCTGGAA-3¢) The PCR conditions were 95 °C for 10 min, 40 cycles of denaturation at 95 °C for 15 s, and annealing and extension at 56 °C for All reactions...

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báo cáo hóa học: " Treatment with gelsolin reduces brain inflammation and apoptotic signaling in mice following thermal injury" potx

Ngày tải lên : 19/06/2014, 22:20

... microglial density and activation status in brain at 72 h pb, implicating delayed activation of microglial proliferation and/ or activation responses after thermal injury Caspase-3 activation in the ... cerebral inflammatory lesions and apoptosis via acceleration of recruitment of monocytes and down-regulation of phospho-ERK1/2 expression, and also via improvement of peripheral T cell functions ... before being sacrificed All experimental manipulations were undertaken in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals, with the approval of...

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Báo cáo hóa học: " Resveratrol engages AMPK to attenuate ERK and mTOR signaling in sensory neurons and inhibits incision-induced acute and chronic pain" pptx

Ngày tải lên : 21/06/2014, 19:20

... resveratrol for h followed by or h washout periods Resveratrol led to a reversible activation of AMPK (Figure 3A) and a reversible inhibition of both ERK (Figure 3B and C) and mTOR signaling (Figure ... nociceptors and provide further evidence for activation of AMPK as a novel treatment avenue for acute and chronic pain states Background Incision associated with surgery causes acute pain and surgery ... Hence, resveratrol dose- and time-dependently activates AMPK and inhibits ERK and mTOR signaling in a reversible fashion in sensory neurons Figure Suppression of ERK and mTOR signaling by resveratrol...

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Báo cáo y học: "Activation of WNT and BMP signaling in adult human articular cartilage following mechanical injury" potx

Ngày tải lên : 09/08/2014, 08:22

... activation of BMP signaling independently of the expression of one ligand, including secretion and solubility of the ligand( s), its/their binding to matrix molecules, the presence of secreted or ... percent of positive cells for phospho-SMAD-1/-5; relative gene expression normalized for the housekeeping β actin gene; percent of the day time point for BMP-2, MMP-3 and MMP-13 mRNA; and percent of ... net activation of the canonical WNT pathway by performing gene expression analysis of the WNT target genes Axin-2 [31] and c-JUN [32,33] Consistent with our hypothesis and with the activation of...

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Báo cáo khoa hoc:" VEGF receptors on PC12 cells mediate transient activation of ERK1/2 and Akt: comparison of nerve growth factor and vascular endothelial growth factor" doc

Ngày tải lên : 11/08/2014, 08:20

... transient activation of ERK1/2 and Akt after In contrast, NGF produced a stronger and persistent phosphorylation of ERK1/2 and Akt than VEGF165 much more pronounced and prolonged activation of downstream ... and Akt activation are sustained for 79.5 h in the presence of NGF (Fig 3, lane 1) Removal of NGF for 7.5 h after 72 hours of differentiation led to a significant reduction of ERK1/2 and Akt ... Experiments were performed in triplicate Results Differential cell binding of VEGF and endostatins To determine the expression profile of binding partners for murine VEGF164, endostatin and the collagen...

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Báo cáo y học: "Global analysis of alternative splicing regulation by insulin and wingless signaling in Drosophila cells" ppt

Ngày tải lên : 14/08/2014, 21:20

... Summary of regulated junctions and genes detected upon activation of insulin and wingless pathways (c) Distribution of classes of alternative splicing events for all Drosophila genes (left) and for ... Activation of insulin and wingless signaling pathways in Drosophila S2 cells Figure Activation of insulin and wingless signaling pathways in Drosophila S2 cells (a) Schematic representation of ... by representsfor removal the of enriched changes inreguways.biological of (top) for alternative and wingless insulinfor latedof promoters andlevel bywingless (bottom) by genes that for eachtablevalues...

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Báo cáo khoa học: "Immunohistochemical Localization of Nerve Growth Factor,Glial Fibrillary Acidic Protein and Ciliary Neurotrophic Factor in Mesencephalon, Rhombencephalon, and Spinal Cord of Developing Mongolian Gerbil" ppsx

Ngày tải lên : 07/08/2014, 15:20

... the formation of the neuronal and glial pathways Different neurotrophic factors and proteins affected neurons and glia during developmental In the former study regarding the distribution of NGF-, ... PND1 (Fig 5D) and PND2 (Fig 1H), the number of NGF-IR increased, which increased the intensity of positive neurons This is in contrast to that observed in the white and gray matter of the spinal ... on the margin of the aqueduct and the 4th ventricle The cortex of the midbrain proper was Immunohistochemical Localization of Nerve Growth Factor, Glial Fibrillary Acidic Protein and Ciliary Neurotrophic...

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Báo cáo khoa học: "In vitro and in vivo gene therapy with CMV vector-mediated presumed dog b-nerve growth factor in pyridoxine-induced neuropathy dogs" pot

Ngày tải lên : 07/08/2014, 23:22

... was performed to combine the PCR products of the partial dβ-NGF using NGF-1F and NGF-2R This overlap PCR was o o performed for 35 cycles at 94 C for min, 58 C for o and 72 C for The size of this ... C for min, 44 C for 1min and 72 C for (Fig 1) PCR using the primers NGF-2F (5'-AGTTCT CGGTGTGCGACAG-3') and NGF-2R (5'-GCCCAGGA GAGTGTGGAG-3') was also performed for 35 cycles at o o o 94 C for ... 94 C for min, 55 C for and 72 C for (Fig 1) A PCR product of approximately 600 bp was expected when using the primers NGF-1F and NGF-1R and 400 bp with the primers NGF-2F and primer NGF-2R Overlapping...

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Báo cáo y học: " Aging, osteoarthritis and transforming growth factor-β signaling in cartilage" pps

Ngày tải lên : 09/08/2014, 07:20

... methods were employed TGF-β isoforms 1, 2, and 3, TGF-β receptors I and II, Smads 2, 3, 4, 6, and 7, and phosphorylated Smad (Smad-2P) were localized in the joints of young and old mice In 2-yearold ... Vol No Reddi R-Smads and the C-Smad have a highly conserved Mad homology domains (MH in the amino terminus and MH in the carboxy terminus) References Binding of the TGF-β isoforms to the TGF-β ... the expression of TGF-β2, TGF-β3 and TGF-β receptor It is noteworthy that the number of cells expressing phosphorylated Smad decreased in old mice In addition, blockade of TGF-β signaling by the...

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Báo cáo y học: "Nerve growth factor and receptor expression in rheumatoid arthritis and spondyloarthritsi" potx

Ngày tải lên : 09/08/2014, 14:21

... denaturation at 50°C for two minutes and 95°C for 10 minutes, 45 PCR reaction cycles were performed each at 95°C for seconds and 60°C for one minute The following mRNA transcripts and assays were ... expression levels of the four neurotrophic ligands and the four receptors as determined in the SF cells are depicted in Table and outlined in detail by scatter plots in Figure (ligands) and Figure (receptors) ... plots showing mRNA expression of the neurotrophic ligands The scatter plots a to d depict the expression levels of the four neurotrophic ligof the neurotrophic ligands ands (a) nerve growth factor...

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Báo cáo y học: " Increased expression of matrix metalloproteinase-10, nerve growth factor and substance P in the painful degenerate intervertebral disc" pot

Ngày tải lên : 09/08/2014, 14:22

... Barksby and colleagues [19] describe the activation of Page of (page number not for citation purposes) pro-MMPs by MMP-10 as 'superactivation' as the targets of activation (pro-MMP-1, pro-MMP-8 and ... matrix formation Over time, this results in the breakdown of matrix until the disc loses both height and function, and in a large proportion of cases, there is innervation and initiation of the ... fibronectin, gelatin and collagens III, IV, V and IX [18] In addition to this proteolytic activity, MMP-10 has been shown to play a role in the activation of a number of other members of the MMP family...

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Báo cáo y học: "Functional significance of nerve growth factor and its receptor (TrkA) in inflammatory arthritis" pps

Ngày tải lên : 12/08/2014, 14:21

... ng/mL) and interleukin-1-beta (IL-1β) (1 ng/mL) Data are expressed as mean ± standard deviation Nerve growth factor (NGF), TrkA, and p75 data of unstimulated FLSs were compared with NGF, TrkA, and ... upregulated in FLSs of inflammatory arthritis (Table 1), and NGF is mitogenic to FLSs (Figure 1) These observations suggest an autocrine loop of NGF for FLS proliferation and suggest that dysregulated ... proliferation and suggest that dysregulated production of NGF has the potential to influence the inflammatory and proliferative cascades of PsA and RA Abbreviations ELISA, enzyme-linked immunosorbent...

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Báo cáo y học: " Open Access Growth factor signaling in lung morphogenetic centers: automaticity, stereotypy and symmetry" doc

Ngày tải lên : 13/08/2014, 13:20

... These may include ligand expression, proteolytic activation of latent forms of ligand, ligand binding to matrix bound and/ or soluble inhibitors, as well as ligand binding to receptor presentation ... intercellular peptide signaling [102] The p66Shc and 46 Kda isoforms are differentially regulated towards the end of fetal lung development [41] VEGF isoform and cognate receptor signaling and lung development ... Thrombospondin-1 and β6 integrin play key roles in assembly and activation of the proteolytic complex Free TGF β ligand is bound extracellularly and may be sequestered by Decorin Noggin and Gremlin...

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Tài liệu Báo cáo khoa học: Osmotic stress sensing and signaling in ﬁshes doc

Ngày tải lên : 18/02/2014, 16:20

... salinity changes and activate a signaling network that integrates the information received from different osmosensors, ampliﬁes this information, and turns on ⁄ off a large number of appropriate ... role of aquaporins as effector proteins of osmosensory signal transduction pathways and the potential role of these proteins in osmosensing of ﬁsh cells has yet to be addressed As a result of recent ... conservation of orthologs of the putative osmosensors TRPV4 and aquaporin and the osmosensory signal transcription proteins TonEBP and TSC22D2 in vertebrates The human sequences of TRPV4 (871...

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Tài liệu Báo cáo khoa học: Osmosensing and signaling in the regulation of mammalian cell function docx

Ngày tải lên : 18/02/2014, 16:20

... cholesterol-enriched microdomains of the plasma membrane (caveolae), intracellular organelles, ligandindependent activation of growth factor- and cytokine receptors and autocrine stimulation of signal transduction ... sites (‘osmosignaling’) Anisoosmotically exposed cells and tissues were frequently used as a model in order to study osmosensing and osmosignaling By this approach, the activation of signaling ... Src-dependent activation of the p38-type MAPK and thereby inhibition of 5800 autophagic proteolysis [14] Thus, integrin-dependent cell volume sensing and signaling integrates into the overall context of...

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Tài liệu Báo cáo khoa học: Dual P2Y12 receptor signaling in thrombin-stimulated platelets – involvement of phosphoinositide 3-kinase b but not c isoform in Ca2+ mobilization and procoagulant activity pdf

Ngày tải lên : 18/02/2014, 16:20

... isoform is responsible for a considerable amount of the PIP3 formed in platelets [21], this may suggest that the PIP3 produced by this isoform leads to plasma membrane binding and, hence, activation ... prolongation of phospholipase C activation and InsP3 production (see scheme in supplementary Fig S1) The data thereby reveal a novel function for the b-isoform of PI3-K Previously, this isoform had ... equation of Fluo-3 for Ca2+ [27] Ultra-pure calcium-free water was used for preparation of all buffers and (ant)agonists Measurement of cytosolic cAMP and InsP3 Intracellular levels of cAMP and InsP3...

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Tài liệu Báo cáo khoa học: Regulation of connective tissue growth factor (CTGF/CCN2) gene transcription and mRNA stability in smooth muscle cells Involvement of RhoA GTPase and p38 MAP kinase and sensitivity to actin dynamics docx

Ngày tải lên : 19/02/2014, 16:20

... cells in the absence of inhibitors Fig Eﬀects of RhoA, Cdc42 and Rac1 on the expression of the CTGF/ CCN2 gene (A) Immunoblot analyses of RhoA, Cdc42 and Rac1 activation by S1P and FBS Cells were ... serum- and S1P-induced CTGF/CCN2 expression signaling overlap, albeit to various extent, at the level of p38 signaling, but are all independent of both Erk1/2 and JNK1/2 signaling pathways The signaling ... the dominant negative form of Rac1 The relatively potent activation of the endogenous CTGF/CCN2 gene by the active mutants of RhoA and Cdc42 may simply reﬂect the ability of Rho GTPases when activated...

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