properties of the protein
Báo cáo khóa học: Structural properties of the protein SV-IV potx
Ngày tải lên :
07/03/2014, 14:20
... the total number of charges measured by electrospray MS Theoretical mass is the mass calculated on the basis of the protein amino-acid sequence The relative abundance refers to the ratio of the ... a-helix in the whole protein, and the 1–70 region is poorly structured SDS increases the a-helix content of proteins revealing the helical potential The a-helix content of the monomeric form of SV-IV ... acetylation at one of the three residues, because the signal of the triacetylated species was less intense than that of the diacetylated species The MS/MS Ó FEBS 2003 Structural properties of SV-IV (Eur...
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Báo cáo khóa học: Functional properties of the protein disulfide oxidoreductase from the archaeon Pyrococcus furiosus A member of a novel protein family related to protein disulfide-isomerase doc
Ngày tải lên :
16/03/2014, 16:20
... the presence of the nucleotide These experiments indicate that the binding and/or hydrolysis of ATP not have any effect on the conformation of PfPDO, possibly because of the localization of the ... using PDI However, the temperature of the assay, which is limited by the stability of the protein substrate RNase, is very far from the optimal growth temperature of the hyperthermopilic micro-organism ... of the native protein FarUV CD spectra in the presence and absence of ATP (data not shown) gave the same results as the spectrofluorimetric analysis, i.e no change in the conformation of the protein...
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Báo cáo Y học: Properties of the Na+/H+ exchanger protein Detergent-resistant aggregation and membrane microdistribution potx
Ngày tải lên :
08/03/2014, 16:20
... amount at the 105–110 kDa size The amount of 105–110 kDa protein was reduced and in many instances evidence of aggregation was evident at the top of the gels The amount of aggregate present at the ... b-cyclodextrin-treated cells of the Na+/H+ exchanger, biochemical characterization of the properties of the full length protein has proven difficult because of its low abundance and because of the difficulty ... that could either alter the distribution of the Na+/H+ exchanger or the constituency of the lipid rafts, we used an established detergent-free lipid raft isolation procedure for some of our studies...
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Báo cáo Y học: Electrostatic properties of the structure of the docking and dimerization domain of protein kinase A IIa doc
Ngày tải lên :
08/03/2014, 22:20
... Cc of Asp and Cd of Glu, and Og of Tyr Positive unit charges were added at the following atoms: Nt of the N-terminus, Nf of Lys, Cf of Arg, and Nd or Ne of His (depending on the position of the ... in the bottom face of RIIa D/D at the monomer interface, which is part of the protein core Figure 4D–F shows the ribbon model of the best structure of RIIa D/D in the same orientations as their ... hydrophobic interface of the two monomers (Fig 6B) A further rotation of the orientation of RIIa D/D of Fig 4B by 180° about the x-axis shows the bottom face of RIIa D/D (Fig 4C) There is some hydrophobic...
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Báo cáo khoa học: Biochemical properties of the human guanylate binding protein 5 and a tumor-specific truncated splice variant Mark Wehner and Christian Herrmann doc
Ngày tải lên :
15/03/2014, 10:20
... account) The size of the protein complex is only slightly increased when nucleotide-free or in the presence of GTP As with hGBP5ta, the complex is increased to the size of a tetramer after binding of ... for the lack of GMP formation (see Fig S2) The similar hydrolytic activities of the a ⁄ b and C-terminally truncated forms of hGBP5 are in contrast to that of the analogous deletion mutant of ... manner, the entropic contributions of nucleotide binding and the coupled oligomerization show opposing signs In the case of hGBP1, release of water from the nucleotide binding pocket and the protein...
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Báo cáo sinh học: " The Severe Acute Respiratory Syndrome (SARS)-coronavirus 3a protein may function as a modulator of the trafficking properties of the spike protein" docx
Ngày tải lên :
18/06/2014, 22:20
... understood The subject of this hypothesis relate to the S protein and one of the "accessory" proteins, the SARS-CoV 3a protein The S protein, which forms morphologically characteristic projections on the ... Furthermore, the juxtaposition of the YxxΦ motif and a ExD (diacidic) motif was found to be essential for the transport of 3a to the cell surface, consistent with the role of these motifs in the ... the plasma membrane into the endosomes Interaction between the adaptor protein complex (AP-2) with the YxxΦ motif present in the cytoplasmic domain of the internalizing protein concentrated the...
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báo cáo hóa học:" The Severe Acute Respiratory Syndrome (SARS)-coronavirus 3a protein may function as a modulator of the trafficking properties of the spike protein" docx
Ngày tải lên :
20/06/2014, 04:20
... understood The subject of this hypothesis relate to the S protein and one of the "accessory" proteins, the SARS-CoV 3a protein The S protein, which forms morphologically characteristic projections on the ... Furthermore, the juxtaposition of the YxxΦ motif and a ExD (diacidic) motif was found to be essential for the transport of 3a to the cell surface, consistent with the role of these motifs in the ... the plasma membrane into the endosomes Interaction between the adaptor protein complex (AP-2) with the YxxΦ motif present in the cytoplasmic domain of the internalizing protein concentrated the...
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Báo cáo khoa học: " Characterization of the RNA-binding properties of the triple-gene-block protein 2 of Bamboo mosaic virus" pdf
Ngày tải lên :
12/08/2014, 04:21
... to the RNA, resulting in quenching of the tyrosine fluorescence We then studied the effect of changing the molar ratio of the viral RNA fragment to TGBp2 on the tyrosine fluorescence quenching ... property of TGBp2, which is responsible for the non-specific interaction between TGBp2 and viral RNA On the basis of the known topological properties of TGBp2 [9], we propose that the self-assembly of ... that of the same protein residing in lipid bilayers [18,19] In other words, the two transmembrane helices of TGBp2 are supposed to be bound by Triton X-100 And the two tyrosine residues in the...
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Báo cáo y học: "What properties characterize the hub proteins of the protein-protein interaction network of Saccharomyces cerevisiae" pot
Ngày tải lên :
14/08/2014, 16:21
... fraction The protein- protein interaction network is susceptible to targeted attacks on the hubs of the network [7,23] Since hub proteins are pivotal for the robustness of the protein- protein network, ... hubs for the topology of the network [14] Further, while there is no substantial difference between the proportion of essential proteins among the party and date hubs, perturbation of the latter ... fraction of proteins with high numbers of interactions that evolve slower than most proteins in the yeast network [12] The difference between some of these studies seems to be due to the nature of the...
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Thermal properties of the vernacular buildings envelopes: the case of the "Sassi di Matera" and "Trulli di Alberobello
Ngày tải lên :
05/09/2013, 14:58
... characteristics of the "Sassi of Matera" and "Trulli of Alberobello" In the South of Italy, the sites of the "Sassi of Matera" (Figure 1a) (classified as humanity world heritage in 1993) and "Trulli of Alberobello" ... if the present structures are characterized with local materials, namely, “Tufo of Matera” for the structures of the "Sassi of Matera," and “Stone of Fasano” for the structures of the "Trulli of ... and the knowledge of the temperature coefficient of the material of which it is made A single measure allows for the determination of diffusivity, thermal conductivity, and specific heat of the...
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Tài liệu Psychometric properties of the quality of life scale Child Health and Illness Profile-Child Edition in a combined analysis of five atomoxetine trials pdf
Ngày tải lên :
12/02/2014, 19:20
... the SH sub-domain (item 1: ‘‘How often does your child have a lot of fun?’’ and item 4: ‘‘How often does your child feel happy?’’) The items of the SPS sub-domain Psychometric properties of the ... smaller The RA and the PA sub-domains had lower correlations with their domains than most of the other domains at baseline, at endpoint, and Psychometric properties of the CHIP-CE also for the change ... use the sub-domains rather than the domains of the CHIPCE when evaluating ADHD patients This is supported by the factor analysis based on the sub-domains and the correlation analysis of the sub-domains,...
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Tài liệu Báo cáo khoa học: Toggle switches, pulses and oscillations are intrinsic properties of the Src activation/deactivation cycle doc
Ngày tải lên :
18/02/2014, 11:20
... by presenting the pulse of Src activity in the plane of the inactive and active fractions, si and s1 If the duration of the stimulus exceeds the critical value, the trajectory in the (si, s1) ... state (in the bistability domain) Regardless of the slope, the active Src fractions increase during the Off to On transition, whereas the value of the inactive fraction (si) decreases if the slope ... all steady states of the Src cycle When there are three intersections, the steady state O1 at the lower branch of the Z-shaped curve (i.e low Src activity) and the state O3 at the upper branch...
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Tài liệu Báo cáo khoa học: Effect of ionic strength and oxidation on the P-loop conformation of the protein tyrosine phosphatase-like phytase, PhyAsr docx
Ngày tải lên :
18/02/2014, 18:20
... a loss of activity (approximately 20% after 10 min) If the inactivation of the protein is due to the formation of a stable sulfenic acid, sulphenyl-amide, or disulfide, then the addition of a reducing ... serve to provide room for the larger oxidized cysteine The key feature that dictates whether the P-loop moves upon oxidation of the catalytic cysteine is the ability of the conserved active site ... contact with the carbonyl oxygen of Gly255 These movements away from the oxidized cysteine increase the space inside the P-loop to accommodate the large sulfonic acid group The movements in the P-loop...
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Tài liệu Báo cáo khoa học: Properties of the recombinant glucose⁄galactose dehydrogenase from the extreme thermoacidophile, Picrophilus torridus ppt
Ngày tải lên :
19/02/2014, 16:20
... containing the structural zinc at the mouth of the cleft and thus exposed to the solvent [8] Gradual depletion of the enzyme first of the structural and then of the catalytic zinc has also been ... However, the addition of ZnCl2 to the incubation buffers showed a marked effect on the stability of the enzyme at both high temperature and acidity This effect was the same across the range of ZnCl2 ... rate under the conditions present in the cytoplasm of Picrophilus The kinetics of NADPH degradation was followed by measuring the rate of decrease of its absorbance at 340 nm over the pH range...
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Tài liệu Báo cáo Y học: Electrochemical, FT-IR and UV/VIS spectroscopic properties of the caa3 oxidase from T. thermophilus docx
Ngày tải lên :
21/02/2014, 15:20
... cooperativity of the other redox centers In order to discriminate the contributions of the cofactors, inhibitors uncoupling or changing the cooperativity can be used Addition of cyanide strongly shifts the ... spectra and the deconvolution of the amide-I region A particular problem of the assignment in the difference spectra is the superposition of signals from different constituents of the oxidase, ... modes on the basis of the data presented here, in spite of the fact that bands in the difference spectra are observed in the region where the modes were attributed The vibrational modes of bound...
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Tài liệu Báo cáo Y học: Short peptides are not reliable models of thermodynamic and kinetic properties of the N-terminal metal binding site in serum albumin doc
Ngày tải lên :
22/02/2014, 04:20
... represent the native HSA sequence and Val-Ile-His-Asn (VIHN), the N-terminal peptide of another blood serum protein, des-angiotensinogen [11] The structure of the Ni(II) complex of the latter ... basicities of nitrogen donors at the protein surface, limited accessibility of the binding site due to shielding from the bulk of the protein, or some conformational interactions The metal-free ... below) The comparison of CD spectra of complexes also points toward slight differences in the conformation of the chelate rings The characteristic alternate pattern of the dd bands in the CD...
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Tài liệu MECHANICAL PROPERTIES OF THE HEART AND ITS INTERACTION WITH THE VASCULAR SYSTEM ppt
Ngày tải lên :
22/02/2014, 09:20
... as the pressure of the left upper corner of the loop; the significance of this pressure will be discussed in detail below Moving to the bottom of the loop, we can reason that the pressure of the ... instantaneous atrial volume) The pressure of the point at the bottom right corner of the loop is the pressure in the ventricle at the end of the cardiac cycle and is called the end-diastolic pressure ... mechanical properties by plotting the time course of change in the slope of the instantaneous PVR Above, we referred to the slope of the ESPVR as an elastance Similarly, we can refer to the slopes of the...
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Báo cáo khoa học: Structure analysis of the flavoredoxin from Desulfovibrio vulgaris Miyazaki F reveals key residues that discriminate the functions and properties of the flavin reductase family pdf
Ngày tải lên :
07/03/2014, 02:20
... b sheet with the b12 of the other monomer, and towards the C-terminus, the subsequent residues, Phe182Lys186, pass through the a2 vicinity of the other monomer For the FeR dimer, the N-terminal ... Resolution of the structure of NADP+-bound FeR revealed that the nicotinamide moiety of NADP+ faces the re-side of the isoalloxazine ring, and that the 2Â-P-AMP moiety is held in the groove between the ... in the binding of the phosphate group(s) of FMN or FAD Lys92 forms a salt bridge with the O3P of FMN A salt bridge that involves avin species has not been reported in the structures of the other...
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Báo cáo khoa học: Effect of oculopharyngeal muscular dystrophy-associated extension of seven alanines on the fibrillation properties of the N-terminal domain of PABPN1 pot
Ngày tải lên :
07/03/2014, 11:20
... domain of PABPN1 consisting of amino acids 1–125 of the wild-type protein (N-WT) Because the aim of this study was to investigate the effect on the fibrillation properties of the most extreme disease-associated ... that the increase in ANS fluorescence correlates with the increase in fibrillar species at the expense of monomeric protein According to the hypothesis that seeded fibril formation in the case of the ... segment [13,14] The poly l-alanine extensions affect the N-terminal fragment of the protein, which comprises 125 amino acids In the wild-type protein, the sequence (Ala)10Gly(Ala)2 follows the start...
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Báo cáo khoa học: A single mismatch in the DNA induces enhanced aggregation of MutS Hydrodynamic analyses of the protein-DNA complexes pot
Ngày tải lên :
07/03/2014, 12:20
... particles within the first and slowly increasing further beyond 400–500 nm, the limit of detection by DLS, as a function of time The width of distribution of Rh was in the range of % 50 nm in these samples ... supporting the MutS–MutL foci formation suggests the possibility of extensive recruitment of protein molecules at the sites of mismatch repair, thereby achieving high enough local concentration of protein ... added either at the start or at the 20-min time-point of the reaction Interestingly, addition of salt at the start of the reaction essentially abrogated mismatch induced discrimination of MutS...
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