... Trong miền tần số: phương pháp Fourier Transform. - Trong miền thời gian và tần số: STFT( Short TimeFourier Transform) . 2.4.1. Phương pháp Fourier: 2.4.1.1. Biến đổi Fourier: Cho một hàm f(t) ... được một biến đổi Fourier cục bộ, chúng ta có thể định nghĩa một biến đổi Fourier cửa sổ. Tín hiệu đầu vào được nhân với một hàm cửa sổ W(t - τ) và sau đó lấy biến đổi Fourier của nó. Kết ... đổi Fourier của nó được định nghĩa: () () ()tfedtetfFtitj,ωωω ==∫∞∞−− (2.14) Biến đổi Fourier ngược là: () ()∫∞∞−= ωωπωdeFtftj21 (2.15) Giả sử rằng biến đổi Fourier...
... changes over time impossible. The objectives ofthe present combined analysis were toevaluate the psychometric propertiesofthe CHIP-CE atbaseline and over time and to assess the correlationbetween ... touse the sub-domains rather than the domains ofthe CHIP-CE when evaluating ADHD patients. This is supported by the factor analysis based on the sub-domains and the correlation analysis ofthe ... and the core symptoms. The influ-ence ofthe QoL ofthe parents or the parents’ diseases(such as ADHD) could not be assessed because these datawere not obtained.Conclusions The strength of...
... presenting the pulse of Src activity in the plane of the inactive and active fractions, siand s1. If the duration ofthe stimulus exceeds the critical value, the trajectory in the (si, ... 2009 The Authors Journal compilation ê 2009 FEBS parameters and, for clarity of exposition, we present the analysis ofthe Src cycle in terms of Eqns (6–8).Intrinsic regulatory propertiesofthe ... steady states ofthe Src cycle. When there are threeintersections, the steady state O1at the lower branch of the Z-shaped curve (i.e. low Src activity) and the stateO3at the upper branch...
... cleft, the putative catalytic zinc being at the bottom ofthe cleftand a lobe containing the structural zinc at the mouth of the cleft and thus exposed to the solvent [8]. Grad-ual depletion ofthe ... However, the addition of ZnCl2to the incubationbuffers showed a marked effect on the stability of the enzyme at both high temperature and acidity. Thiseffect was the same across the range of ZnCl2concen-trations ... Novagen.Expression of Pt-gdh under the control of araB promoterwas induced for 4 h at 30 °C by the addition of 0.2% ara-binose when the A600 of the growing culture reached 0.5. The cells from...
... to the different environ-ment ofthe heme centers. The difference signals observed at442 nm and 603 nm can be assigned to the contributions of the hemes a and a3, with the position ofthe ... modes on the basis ofthe datapresented here, in spite ofthe fact that bands in the difference spectra are observed in the region where the modes were attributed. The vibrational modes of bound ... particular problem ofthe assignment in the differencespectra is the superposition of signals from differentconstituents ofthe oxidase, which can lead to the possibility of multi component...
... solution by the rest ofthe protein. There is no correlation between the complex stability and the rate of its formation. The Ni(II) for Cu(II) exchange rates f or peptides are o f the order of 10)6s)1in ... albumins. On the other hand, the directthermodynamic and kinetic characterization of Ni(II)binding at site I in HSA and BSA was obtained. Thesedata can be very useful in further studies ofthe toxicolo-gically ... values of *K, the equilibrium constant ofthe actual complexformation reaction:M ỵ HnL ẳ MHjL ỵnỵjịHỵThis constant represents the overall ability of ligand L toform a given complex. The other...
... characterize thetime course of change in ventricular mechanical properties by plotting thetime course of change in the slope ofthe instantaneous PVR. Above, we referred to the slope ofthe ESPVR ... interaction between the ongoing contraction process ofthe cardiac muscles and thepropertiesofthe arterial system and is beyond the scope of this lecture. As the contraction process ofthe cardiac ... contraction there is a period oftime during which the mechanical properties ofthe heart are characterized by the EDPVR; knowledge ofthe EDPVR allows one to specify, for the end of diastole,...
... reverse-phase HPLC on a C8column,and the retention timeofthe obtained prosthetic groupwas compared with those of flavin derivatives. The retention timeofthe prosthetic group bound to recom-binant ... ofthe structure of NADP+-bound FeRrevealed that the nicotinamide moiety of NADP+faces the re-side ofthe isoalloxazine ring, and that the 2Â-P-AMP moiety is held in the groove between the ... direction along the b10 ofthe other mono-mer, forming an anti-parallel intermolecular b sheet(Fig. 2A). The subsequent residues ofthe loop (Ala7–Pro13) turn into the interior ofthe dimer interface,and...
... alanines are likely. The manner in which the number of alanines determines the atomic structure ofthe fibril and the b-strandarrangements remains to be determined.Yet, knowledge ofthe structure and ... using the nanoscope analysis software.Chemical stability of fibrils The chemical stability of fibrils was tested after the fibrilla-tion process was completed (no further rise of ANS sig-nals). The ... consisting primar-ily of PABPN1. Poly l-alanine-dependent fibril formation was studied using the recombinant N-terminal domain of PABPN1. In the case ofthe proteinfragment with the expanded poly...
... identified by the massincrease of 42 mass units. The relative level of acetylation of a peptide was estimated on the basis ofthe intensity ratio of the native and acetylated species. From the data ... a-helix in the whole protein, and the 1–70region is poorly structured.SDS increases the a-helix content of proteins revealing the helical potential. The a-helix content ofthe monomericform of SV-IV ... Lys80,showed partial acetylation at one ofthe three residues,because the signal ofthe triacetylated species was lessintense than that ofthe diacetylated species. The MS/MSFig. 2. Secondary-structure...
... the ferrous state ofthe mutant may contribute to anincrease in the disorder and hence entropy of the reduced state ofthe mutant compared to that of the wild-type protein.In contrast to the ... disposition of the proximal His and Asp residues, affecting the imidazo-late character ofthe His, but also by the formation of a LS species. The latter indicating the probable bind-ing of His42 to the ... impacton thepropertiesof His170. The strength ofthe hydro-gen bond between the proximal His and Asp residues,and thus the imidazolate character ofthe His is expec-ted to modulate not only the...
... [1,4]. The protein is known to exist as a dimer and the NHE1 isoform specifically dimerizes to itself and not toother isoforms ofthe Na+/H+exchanger [5].Although the NHE1 isoform ofthe Na+/H+exchangeris ... concentrations ofthe fractions, together with the relative amount of Na+/H+exchanger present in the supernatant and the pellet [19], it was found that over 80% of the Na+/H+exchanger remained in the ... that couldeither alter the distribution ofthe Na+/H+exchanger or the constituency ofthe lipid rafts, we used an establisheddetergent-free lipid raft isolation procedure for some of ourstudies...
... the site of interaction with AKAP proteins [5,6]. The symmetry axis of the dimer is along the y-axis shown in the Figure. Figure 4Bshows the van der Waals model ofthe top face ofthe beststructure ... A further rotation ofthe orientation of RIIaD/D of Fig. 4B by 180° about the x-axis shows the bottomface of RIIa D/D (Fig. 4C). There is some hydrophobiccharacter in the bottom face of RIIa ... bar at the C-terminus of eachhelix corresponds to the variable helical range within the ensemble of the 24 structures. There is no variation at amino terminus of each helix. The sequence of ionizable...
... con-served in the primary structure ofthe GTPase domain of hGBP5, so the formation of GMP must be impairedin an as yet unknown manner. Sequence alignments of the large GTP-binding domains of hGBP1, ... for the lack of GMP formation(see Fig. S2). The similar hydrolytic activities of the a ⁄ b and C-terminally truncated forms of hGBP5 are incontrast to that ofthe analogous deletion mutant of hGBP1 ... account). The size ofthe protein complex is onlyslightly increased when nucleotide-free or in the presence of GTP. As with hGBP5ta, the complex isincreased to the size of a tetramer after binding of GppNHp,...
... ofthe PDI [42], and t hat this site is far away from the redox active sites in the sequ ence. Furthermore, the measurements ofthe rates of PDI-catalysed refolding of scrambled RNase A, in the ... (225 mM)9:1(25 mM). At the end ofthe incubation, 40 lL ofthe mixturewas added to each ofthe five solutions; these were then leftto react on ice for 5 min. The reaction mixtures w ereanalysed ... ofthe active site in the N-unit. As regards the oxidative activity, the two units alsodisplay differences in their functional properties, with the site at the C-termus always predominant, the...