properties calculated from the 3d structure of the ligand amp 8211 macromolecule complex
Báo cáo hóa học: "Recovery and Visualization of 3D Structure of Chromosomes from Tomographic Reconstruction Images" potx
Ngày tải lên :
22/06/2014, 23:20
... in detail the various steps of our methodology and show results of the visualizations of the reconstructed Drosophila chromosomes METHODOLOGY 2.1 Overview of the approach The objective of our research ... estimate for the contour refinement process The image influence Eimage is obtained based on the image gradient computed on the Gaussian smoothed image An example of the initial contour of the snake ... sample input images on the right are from the set of tomographic reconstruction volume images The total number of slices is 65, and is greyscale From left to right, the image samples are slice number...
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Báo cáo y học: " Crystal structure of an FIV/HIV chimeric protease complexed with the broad-based inhibitor, TL-3" docx
Ngày tải lên :
13/08/2014, 09:20
... a result, the structure of the complex is an average of the two half-sites Similarly, TL3 was bound in the active site of the 12X FIV PR with its C2 axis of symmetry coincident with the crystallographic ... K63I in the flap region, and L97T, I98P, Q99V, P100N, and L101I in the "90s loop" region The 2-fold axis of the 12X FIV protease dimer is vertical in the plane of the figure; the C2 axis of the bound ... includes the two pairs of hydrogen bonds that form critical interactions between the flap regions of the PR and the inhibitor However, the 12X FIV PR complex lacks the water molecule which bridges the...
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NEVANLINNA THEORY FOR MEROMORPHIC MAPS FROM A CLOSED SUBMANIFOLD OF C l TO A COMPACT COMPLEX MANIFOLD
Ngày tải lên :
16/10/2015, 14:07
... assume the hypothesis as in the statement of Theorem 4.3 and also keep the notations as in the first part of the proof of Theorem 4.3 Let f, g be in Aρ (M, X) Set F = Φ ◦ f, G = Φ ◦ g Theorem ... to the unit ball of Cl Motivated by studying meromorphic mappings into compact complex manifolds in [3] and from the point of view of the Nevanlinna theory on polydiscs, the second main aim of ... Theorem 4.7 We still use notations as in the first paragraph of the proof of Theorem 4.3 We now suppose on the contrary By definition of the non-integrated defect, there exist ηi ≥ (1 ≤ i ≤ q) and nonnegative...
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Báo cáo khoa học: H NMR study of the molecular structure and magnetic properties of the active site for the cyanomet complex of O2-avid hemoglobin from the trematode Paramphistomum epiclitum pdf
Ngày tải lên :
23/03/2014, 17:22
... is the angle between the projection of the tilt of the z axis on the xÂ, y plane (dened direction of tilt of z and the x axis), and j % a + c denes the location of the rhombic (x, y) axes The ... The two structures of WT metHb and rWT metHbH2O, exhibit signicant differences in the interaction of the FG loop with the heme and in the position of the B-helix, with Tyr32(B10) closer to the ... of the protein that we have characterized above Thus parts of the FG corner move away from the heme and the B-helix moves closer to the heme in the WT metHb than in the rWT metHbH2O crystal structure, ...
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Tài liệu Báo cáo khoa học: Crystal structure of importin-a bound to a peptide bearing the nuclear localisation signal from chloride intracellular channel protein 4 ppt
Ngày tải lên :
14/02/2014, 19:20
... primarily occurs by the formation of a hydrogen bond between the side-chain hydroxyl group of Tyr205 and the importin-a main chain at the C-terminus of the ARM1 H3 helix The hydrogen bond is ... recalculated The normalized B factors from apo importin-a were then subtracted from those in the importin-a:CLIC4 NLS structure, and the difference was again normalized using the equation above The final ... unfold into an extended conformation Results Structure of the importin-a:CLIC4 NLS peptide complex The structure of importin-a (70–529) bound to the CLIC4 NLS peptide (198VKVVAKKYRN207) was ˚...
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Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf
Ngày tải lên :
14/02/2014, 22:20
... NE2 of His31, bound to the metal, in the company of a water oxygen, from the apical positions The manga˚ nese was only 0.06 A out of the equatorial plane (Table 3) The angles around the metal cofactor ... on the coordination of the metal cofactor in the active site as well as the changes it experiences in response to different metal cofactors Finally, by comparing ApeSOD with the SOD from the thermophilic ... similar; the rmsd of the 747 Ca atoms was ˚ 0.695 A ApeSOD eluted from the gel-filtration column with the elution volume for a molecular mass of 57 kDa (Fig 2A) Because the calculated molecular mass of...
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Tài liệu Báo cáo khoa học: Structure of the putative 32 kDa myrosinase-binding protein from Arabidopsis (At3g16450.1) determined by SAIL-NMR docx
Ngày tải lên :
18/02/2014, 14:20
... constraints Solution structure of SAIL At3g16450.1 Assignment of the NOE peaks of At3g16450.1 and the structure determination were accomplished by use of the cyana program [17,18] The structural statistics ... analysis of At3g16450.1 with sugars Fig Three-dimensional NMR structure of At3g16450.1 (A) Superposition of the 20 energy-minimized conformers that represent the 3D solution structure of the N-terminal ... backbone trace of the NMR structure of the C-terminal domain 1JOT), a plant lectin, as the closest structure The root mean square deviation values for the N- and C-terminal ˚ domains versus the agglutinin...
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Tài liệu Báo cáo khoa học: Crystal structure of the BcZBP, a zinc-binding protein from Bacillus cereus doc
Ngày tải lên :
19/02/2014, 00:20
... gray indicates the C-terminal part The dimer’s formation is established by the incorporation of the b8-strand of one monomer into a b-sheet of the other monomer The position of the zinc ion is ... and determines the shape of the active site entry (e) The structure of the active site is essentially identical with the active sites of the MshB and LpxC proteins The conservation of catalytically ... the presence of acetate in the crystal structure The binding of acetate in the active site is a further indication that the enzyme may be involved in deacetylation because acetate is one of the...
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Tài liệu Báo cáo khoa học: Spectroscopic characterization of a higher plant heme oxygenase isoform-1 from Glycine max (soybean) ) coordination structure of the heme complex and catabolism of heme docx
Ngày tải lên :
19/02/2014, 05:20
... information on the heme pocket structure As shown in Table 2, the hyperfine splitting constants of the 15N nucleus of the distal NO and of the 14N nucleus of the proximal His of the GmHO-1 complex are ... coordination of a histidinyl residue to the proximal site of the heme The spectral features of 15NO-heme–GmHO-1 are somewhat different from those of the nitrosylheme complexes of SynHO-1 (Fig 3D) and ... Expansion of the gxy region of (A) (solid line) and of the corresponding part of the spectrum of ferric heme–rHO-1 (dotted line) a-2, Expansion of the higher-field region of (A) (B) The ferric...
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Tài liệu Báo cáo khoa học: ˚ The 1.8 A crystal structure of a proteinase K-like enzyme from a psychrotroph Serratia species docx
Ngày tải lên :
19/02/2014, 07:20
... arrangement, and the side chain of Ser97 in VPRK is too short One side of the loop is involved in the binding of the P2–P4 residues of a substrate The other side of the loop is close to Arg94 (Lys ... the carbonyl oxygen atom of the scissile bond in other subtilase–inhibitor complexes (data not shown) The Ca positions of the tripeptide occupy almost the same positions as the Ca positions of ... a residue forming a part of the S2 site of the enzyme The disulfide bridge restricts the flexibility of a tight loop which is part of the S2 site The tight loop is further stabilized by a tight...
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Tài liệu Báo cáo khoa học: Properties of the recombinant glucose⁄galactose dehydrogenase from the extreme thermoacidophile, Picrophilus torridus ppt
Ngày tải lên :
19/02/2014, 16:20
... containing the structural zinc at the mouth of the cleft and thus exposed to the solvent [8] Gradual depletion of the enzyme first of the structural and then of the catalytic zinc has also been ... UÆmg)1) the remaining activity did not differ from that of the sample incubated in the absence of salts (data not shown) Also, EDTA completely abolished the stabilizing effect of Zn2+ When the enzyme ... Novagen Expression of Pt-gdh under the control of araB promoter was induced for h at 30 °C by the addition of 0.2% arabinose when the A600 of the growing culture reached 0.5 The cells from a 1-L culture...
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Tài liệu Báo cáo khoa học: Crystal structure of thiamindiphosphate-dependent indolepyruvate decarboxylase from Enterobacter cloacae, an enzyme involved in the biosynthesis of the plant hormone indole-3-acetic acid doc
Ngày tải lên :
20/02/2014, 11:20
... However, the packing of the two dimers in the tetramer is different from that of the PDCs of known structure, best described as a 20° rotation of one dimer towards the other when compared to the Z ... catalytic residues The active site cavity in IPDC extends from the thiazolium ring of the cofactor to the surface of the protein The entrance of the active site cleft is covered by the C-terminal ... ScPDC on the other hand involving the C-terminal part of the polypeptide chain (Fig 7) In the latter, differences in the conformation of the loop between Fig Stereo picture of the model of the a-carbanion/enamine...
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Tài liệu Báo cáo khoa học: Complete subunit sequences, structure and evolution of the 6 · 6-mer hemocyanin from the common house centipede, Scutigera coleoptrata pptx
Ngày tải lên :
20/02/2014, 11:20
... instead of Cys) and (Ala instead of Pro) may be explained by the presence of an additional subunit in the sample that most likely corresponds to HcC, as also inferred from the minor sequence in the ... [41,44], although the support values in the present are significantly higher This observation supports the notion of the usefulness of hemocyanin sequences for the resolution of the arthropod phylogenetic ... deduced from the pattern of identical peptide masses The subunits were named HcA to Protein structure modeling Homology models of the S coleoptrata hemocyanin subunits were built applying the online...
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Tài liệu Báo cáo khoa học: The structure of the carbohydrate backbone of the lipopolysaccharide from Acinetobacter baumannii strain ATCC 19606 docx
Ngày tải lên :
21/02/2014, 03:20
... biosynthesis of Escherichia coli Fig The complete chemical structure of the carbohydrate backbone of the LPS from Acinetobacter baumannii strain ATCC 19606 Ó FEBS 2002 [26,27] Then, the biosynthesis ... combined to a complete structure of the carbohydrate backbone of the LPS from A baumannii strain ATCC 19606 as depicted in Fig The structures of four core regions from LPS of different Acinetobacter ... Monosaccharide analyses of the LPS from A baumannii strain ATCC 19606 revealed the presence of D-Glc, D-GlcN, D-GalN, and Kdo The D-con®guration of Kdo was determined on the basis of the optical rotation...
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Báo cáo khoa học: Crystal structure of salt-tolerant glutaminase from Micrococcus luteus K-3 in the presence and absence of its product L-glutamate and its activator Tris pdf
Ngày tải lên :
06/03/2014, 09:22
... for the N structure The G and TG structures were solved using the N structure as a search model, and the TG structure was used in phasing of the T structure The structures were refined using the ... typically increased the free R values by 0.1% Although the R and free R values of the four structures were in the range of the values of the published structures, those of the N and G structures were ... aa) in the T and TG structures are aligned b Backbone atoms of Bacillus glutaminase are superimposed on those of the liganded enzyme c The value of Tyr27 is excluded from the calculation of the...
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Báo cáo khoa học: Structure of coenzyme F420H2 oxidase (FprA), a di-iron flavoprotein from methanogenic Archaea catalyzing the reduction of O2 to H2O ppt
Ngày tải lên :
07/03/2014, 10:20
... between the isoalloxazine ring of FMN and the segment between His151 and Pro153 of the switch loop, whereby the imidazole group of His151 interacts with the bottom of F420H2 and the side chain of ... Ca atoms of the two models) The phases for the other two crystals were obtained by molecular replacement using the model from the air-exposed monoclinic crystals [47] Refinement of the structures ... that transfers hydride Fig Structures of F420H2 and of FMNH2, both viewed from the Si face The Re and Si faces of the flavin isoalloxazine ring are defined relative to C5 of the oxidized deazaflavin...
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Báo cáo khoa học: Solution structure of the catalytic domain of RICH protein from goldfish pot
Ngày tải lên :
07/03/2014, 10:20
... The Authors Journal compilation ª 2007 FEBS 1601 Solution structure of the RICH catalytic domain G Kozlov et al Results Structure of the RICH catalytic domain We determined the structure of the ... orthophosphate identifies orientation of 3¢ -AMP when bound to the catalytic domain of RICH (F) Mapping of the chemical shift changes due to the adenine group of 3¢ -AMP on the RICH structure D T322 0.7 Residue ... (B) Representation of flexibility in the solution structure of the RICH catalytic domain The width of the sausage is reversely proportional to the heteronuclear NOE values The figure was generated...
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Báo cáo khoa học: Detailed structure of lipid A isolated from lipopolysaccharide from the marine proteobacterium Marinomonas vaga ATCC 27119T pot
Ngày tải lên :
07/03/2014, 15:20
... and the other one is the secondary fatty acid at C3 of the amide-linked C10 : (3OH) Based on the above results together with the analyses of chemical composition and NMR data, the structure of ... structural elucidation of lipid A from the marine proteobacterium M vaga To the best of our knowledge, this is the second complete structure of lipid A from a marine bacterium (lipid A from wild-type ... Evidente, A & Surico, G (2002) Determination of the structure of the lipid A from the lipopolysaccharide of Pseudomonas cichorii by means of NMR and MALDI-TOF mass spectrometry Eur J Org Chem 18,...
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Báo cáo khoa học: Solution structure of the active-centre mutant I14A of the histidinecontaining phosphocarrier protein from Staphylococcus carnosus ppt
Ngày tải lên :
07/03/2014, 16:20
... regions Fig Structure ensemble of HPr(I14A) The average structure of the 10 lowest-energy structures out of 300 calculated with CNS is shown The radius of the spline reflects the RMSD values of the Ca ... used for the calculations Therefore, we recalculated the structure of the wild-type protein on the basis of the restraints used previously [15] with the same protocol used here for the Incidence ... computed as the ratio of the standard deviations of the chemical shifts of the amide nitrogen and proton nuclei Results Determination of the three-dimensional structure of HPr(I14A) To allow the comparison...
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Báo cáo khoa học: Unravelling the functional interaction structure of a cellular network from temporal slope information of experimental data docx
Ngày tải lên :
07/03/2014, 21:20
... particular, if fij > then we interpret that the node j activates the node i by increasing the net rate of xi and if fij < then the node j inhibits the node i We note that the dynamics of the system in ... for the in-numero example (A) and a simple real example for the partial interaction structure of the HOG pathway from S cerevisiae (B) for illustration of the present identification method The ... find the functional interaction structure, dt i.e the sign of fij As it is difficult to find out the exact value of dxi from experimental data, we restrict dt ourselves to utilizing only the sign of...
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