prooxidant antioxidant activity of flavonoids

... hydroxyl radicals The ability of the TMP-A to scavenge hydrogen peroxide was determined according to the method of Smirnoff and Cumbes [17] The percentage of scavenging of hydrogen radicals was calculated ... predicted structure of the novel polysaccharide TMP-A was shown in Fig 3.3 Determination of DPPH radical scavenging activity of TMP-A Fig Superoxide radical scavenging effect of TMP-A from Tricholoma ... hydroxyl radical scavenging effects than Vc of same dose 3.6 Antioxidant activity analysis of TMP-A by MTT In MTT experiments we determined protection effect of TMPA on PC12 cell from hydrogen peroxide...

Ngày tải lên: 28/06/2014, 11:20

... Evaluation of In Vitro Antioxidant Activity 80 depends on a common initiating step, the peroxidation of polyunsaturated fatty acid components of LDLs.8 Such modification of LDLs can be inhibited by antioxidants.9,10 ... SD (n = 3) Evaluation of In Vitro Antioxidant Activity 90 antioxidant activity of 5H-dibenz[b,f]azepine and its derivatives on human LDL oxidation and lipid peroxidation activity in the liposome ... (1996) Structure -antioxidant activity relationships of flavonoids and phenolic acids Free Radical Biol Med., 20(7), 933–956 Yen, G C & Chen, H Y (1995) Antioxidant activity of various tea extracts...

Ngày tải lên: 07/08/2014, 14:20

... 3) to test its antioxidant activity Figure The Ugo-Basile activity cage (type 7401) The Ugo-Basile activity cage (type 7401) Materials and Methods Synthesis of TPBIA Key step of the synthesis ... motor activity of the rats was measured between 12-6 pm in an Ugo-Basile activity cage (type 7401) (Figure 2) in vitro The antioxidant potential of TPBIA was investigated in the model of in vitro ... (Number of experimental animals: 4) b) modifies the motor behavior of the experimental animals, c) shows antioxidant activity a) The Polar Surface Area (PSA) of a molecule is defined as the area of...

Ngày tải lên: 08/08/2014, 20:23

... 3,4,7,3’,4’ pentamethoxy flavone (Tuberosin) Assay of antioxidant property a ABTS* radical scavenging activity ABTS* radical scavenging activity of tuberosin was determined according to Re et al ... The powder of PT root-tubers are already in clinical use by Ayurvedic physicians of Indian system of medicine [6], but neither its mechanism of action nor the active principle for its antioxidant ... steps of the signaling cascade of LPS induced NO production, but it is still not clear, whether it is directly inhibiting the activity of iNOS enzyme or it is suppressing the synthesis of this...

Ngày tải lên: 11/08/2014, 03:20

... determination of ferric reducing antioxidant power (FRAP), determination of antioxidant activity with thiobarbituric acid reactive species (TBARS) and automatic determination of the oxidative stability of ... Determination of Antioxidant Activity with the 2,2'-Diphenyl-1-picrylhydrazyl (DPPH) Radical Scavenging Method The antioxidant activity of volatile compounds was measured in terms of hydrogen donating ... absorbance of the control at t = and AA(t) is the absorbance of the antioxidant at t = h Determination of Ferric Reducing Antioxidant Power (FRAP Assay) The total antioxidant potential of a sample...

Ngày tải lên: 16/08/2014, 22:31

... gratitude to Professor Bay Boon Huat of the Department of Anatomy, Yong Loo Lin School of Medicine and Associate Professor Heng Chew Kiat of the Department of Pediatrics, Yong Loo Lin School of Medicine ... University of Singapore, st nd Singapore – of August, 2008 Waisundara, V.Y., et al The antioxidant activity of Scutellaria baicalensis (poster) Waisundara, V.Y., et al Antioxidant effects of Rehmanniae ... occurring flavonoids offer preliminary insight into the impact of these metabolic alterations on various mechanisms of antioxidant activity 2.4 TRADITIONAL CHINESE MEDICINE IN THE PREVENTION OF DIABETES...

Ngày tải lên: 12/09/2015, 10:58

... showed antioxidant activity Thin-layer chromatographic analyses of the extracts showed the presence of phenolic compounds in the crude extracts, two of which were flavonoids The anti-inflammatory activity ... References [1] Alia M, Horcajo C, Bravo L, Goya L Effect of grape antioxidant dietary fiber on the total antioxidant capacity and the activity of liver antioxidant enzymes in rats Nutr Res 2003;23:1251À67 ... N StructureÀradical scavenging activity relationship of flavonoids Croat Chem Acta 2003;76(1):55À61 [3] Oboh G Effect of blanching on the antioxidant property of some tropical green leafy vegetables...

Ngày tải lên: 12/01/2016, 18:24

... oxidative modification of erythrocytes [10] Antioxidants can help in preservation of an adequate antioxidant status; therefore, they preserve the normal physiological function of living cells by ... ability to inhibit the production of ROS as well as maintains the antioxidants activity in the cell [24] Moreover it has been reported that supplementation of antioxidants preserve the NPSH from ... pravastatin maintains catalase activity; however catalase is one of the important antioxidant enzymes in erythrocytes [29] The reduction of NPSH by primaquine is a clear indication of oxidative stress,...

Ngày tải lên: 25/10/2012, 11:40

... antioxidant activity of guest fullerenes To the best of our knowledge, this is the first result of the higher antioxidant activity of C60O in comparison with pristine C60, despite the decreasing of p-conjugation ... introduction of pin-up oxygen on C60 increases its antioxidant activity In order to clarify the reason for the significant effect of the pin-up oxygen on the antioxidant activity of C60, we calculated ... bleaching assay is one of the common methods used in the field of food chemistry for evaluating antioxidant activity The method is based on the discoloration of the yellowish color of a b-carotene solution...

Ngày tải lên: 22/06/2014, 01:20

Ngày tải lên: 28/06/2014, 11:20

... protection Involvement of antioxidant genes with varying kinetic profiles of activity could provide both immediate and more prolonged antioxidant capacity The activity of such a mechanism would ... potential pathways of their antioxidant activity In the case of coconut oil, its phenolic acid constituents have been proposed as the primary sources of its oxidant species scavenging activity [28] ... Han J, Agarwal A, Kong AN: Mechanism of action of sulforaphane: inhibition of p38 mitogen-activated protein kinase isoforms contributing to the induction of antioxidant response element-mediated...

Ngày tải lên: 12/08/2014, 13:22

... mass of sample, g w: moisture 2.5 Verification of antioxidant activity and IC50 index The Antioxidant activity was calculated by: AA% = Abscontrol - Abssample 100% Abscontrol AA%: antioxidant activity ... IC50 index of Basella rubra L was 1.8 µg/mL, profusely lower than garlic acid and ascorbic acid The lower of IC50 the more effective of antioxidant activity, therefore, the antioxidant of Anthocyanin ... DPPH by 50%, therefore, the antioxidant activity of Anthocyanin is higher Since the high antioxidant activity, Basella rubra L can be considered as potential sources of functional foods in prevent...

Ngày tải lên: 06/10/2015, 15:58

... progression in eight different types of cancer, and evaluated the anticancer activity of the PR domain against human hepatoma HuH7 cells through both direct administration of recombinant His6-tagged PR ... was performed using GraphPad InStat (GraphPad Software, San Diego, California, USA) Anticancer activity of PR domain by cDNA transfection The cloning of the PR domain (residues 13-193) has been ... respectively Western blot of PR domain The expression of the PR domain was examined after 24 hr of transfection with pcDNA/HisMax/PR and the empty vector, respectively Supernatants (30 g) of the cell lysates...

Ngày tải lên: 25/10/2012, 11:15

... organization of nitrifying bacterial populations and the in situ activity of these populations within the biofilms was discussed Microsensors For analysis of microbial structure and function (activity) of ... only net chemical profiles, and the spatial resolution is also above a single-cell level To address the question of the higher abundance and activity of SRB in oxic zones of biofilms (Okabe et al., ... performed Fixation and Cryosectioning of Biofilm Samples Biofilm samples were taken at regular time intervals during the periods of the biofilm development The biofilm samples were fixed with freshly...

Ngày tải lên: 05/09/2013, 08:40

... complexity of cell wall Silver ion (Ag+) is a bactericidal agent (Pedahzur et al., 1995) The binding of Ag+ and respiratory enzymes initiates changes in permeability of plasma membranes of bacteria ... standard deviation of independent triplicates - 49 - Journal of Water and Environment Technology, Vol.3, No.1, 2005 Effect of Ag content in photocatalysts Bactericidal activities of the three photocatalysts ... deviation of independent triplicates - 50 - Journal of Water and Environment Technology, Vol.3, No.1, 2005 Increasing the concentration of photocatalysts did not increase the bactericidal activity...

Ngày tải lên: 05/09/2013, 09:08

... structure of the available inhibitors of cathepsin D [19], a lysosomal protease of mammalian cells, and chalcones and phenothiazines have been assayed as inhibitors of falcipain-2 [20,21] Inhibition of ... Discussion Inhibition of proteases by MA It has been previously proposed that MA may inhibit the activity of proteases of T gondii [14] and HIV [12,31] To ascertain the inhibition range of MA on the different ... proteolytic hydrolysis of haemoglobin as a source of amino acids constitutes one of the essential processes which take place along the intra-erythrocytic stage of the parasite Degradation of haemoglobin...

Ngày tải lên: 14/02/2014, 14:20

... hydrolytic activity towards ATP (Fig 1A) The binding of a magnesium cofactor has long been established as essential for the activity of canonical GTP-binding proteins [29,31] The precise role of Mg2+ ... determine the requirement for magnesium of the GTPase activity of TorD, the reaction was performed in varying amounts of MgCl2 (Fig 1B) The GTPase activity of TorD without added MgCl2 is negligible, ... co-ordination of the two distinct functions of TorD with the folding of TorA and possible interactions of both proteins with the MGD biosynthetic apparatus [12] The concentration of GTP in the cytoplasm of...

Ngày tải lên: 16/02/2014, 09:20

... activity by measuring degradation of the Ac-DEVD-pNA peptide (right panel) The graphs represent average cell survival as a percentage of the control and the average fold increase of caspase activity ... induce caspase activity and cleavage of Bid in tBid [30] In our hands, treatment of HEK-293T cells with 200 ngÆmL)1 TRAIL for h led to a significant loss of cell viability (33.1 ± 3.3% of control; ... between the proteins BRET ratios were plotted as a function of the excited GFP activity to total rLuc activity ratio, allowing comparison of BRET ratios between Bid–GFP and tBid–GFP when expressed...

Ngày tải lên: 16/02/2014, 09:20

... detected in the case of mGFP–calpain 7C290S, suggesting that the degradation was caused by proteolytic activity of mGFP–calpain itself We refer to this issue later Effects of mutations of IST1 MIMs on ... interaction enhances the autolytic activity of mGFP– calpain 7–Strep in vitro Autolytic properties of mGFP–calpain 7–Strep We further characterized the autolytic activity of mGFP–calpain 7–Strep Purified ... but not by GST–MIML326D,L353A or GST Enhancement of autolytic activity of mGFP– calpain by calpain 7–IST1 interaction As shown in Fig 3, expression of mGFP–calpain in HEK293T cells generated  45...

Ngày tải lên: 18/02/2014, 04:20

... presence of denaturants [27] Extending these studies, we examine here the effect of increasing the bulk of the residue at position 74 Surprisingly, the Y74W mutant exhibited loss of both activity ... the progress of the reactions of WT enzyme and other mutants is very fast The results are summarized in Fig It is evident that the Y74W* mutant shows an enhancement of activity of 21.9-fold over ... the geometry of the active site The availability of crystal structures of TIMs from 21 sources and the large database of TIM sequences from various sources facilitate an analysis of mutational...

Ngày tải lên: 18/02/2014, 11:20