proliferation cell viability cell cycle and evaluation of cell death
Báo cáo y học: "Expression of cartilage-derived morphogenetic protein in human intervertebral discs and its effect on matrix synthesis in degenerate human nucleus pulposus cells" potx
Ngày tải lên :
09/08/2014, 14:22
... (IAF) and outer annulus fibrosus (OAF) and analysed separately Within each area 200 cells were counted and the number of immunopositive cells expressed as a proportion of this Averages and standard ... receptor and here we relate the expression and distribution of CDMP to that seen previously for the receptor BMP RII [17] Furthermore, the effect of CDMP on cell proliferation, aggrecan and collagen ... investigate the effects of CDMP on cell proliferation, gene expression for aggrecan and type II collagen and proteoglycan production Following isolation, cells were expanded in monolayer culture...
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Báo cáo khoa học: Ribonuclease H: properties, substrate specificity and roles in retroviral reverse transcription ppt
Ngày tải lên :
30/03/2014, 02:20
... SW, Goff S & Baltimore D (1979) A detailed model of reverse transcription and tests of crucial aspects Cell 18, 93–100 Telesnitsky A & Goff SP (1997) Reverse transcriptase and the generation of ... which one strand is entirely DNA and the other strand is RNA at the 5¢-end followed by a stretch of DNA, the HIV-1 and M-MLV retroviral RNases H strongly prefer to cleave the RNA strand one nucleotide ... plus-strand genome, the process of reverse transcription produces a doublestranded DNA product that is integrated into the host cell genome and ultimately serves as a template for the production of...
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Báo cáo sinh học: " Quantitative assessment of the effect of uracil-DNA glycosylase on amplicon DNA degradation and RNA amplification in reverse transcription-PCR" pdf
Ngày tải lên :
19/06/2014, 08:20
... real-time PCR and RTPCR offers significant advantages over standard PCR and RT-PCR for detection of DNA or RNA in terms of reduced sample handling, the time required for analysis and analytical ... elimination of carry-over contamination has led several manufacturers of commercial PCR and RT-PCR reagents to substitute dUTP in the reaction mixture in place of dTTP and in the case of some manufacturers ... 10 15 20 25 30 35 40 Cycle number 10 15 20 25 30 35 40 Cycle number Figure Simultaneous detection of viral RNA and contaminating DNA Simultaneous detection of viral RNA and contaminating DNA...
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báo cáo hóa học:" Quantitative assessment of the effect of uracil-DNA glycosylase on amplicon DNA degradation and RNA amplification in reverse transcription-PCR" ppt
Ngày tải lên :
20/06/2014, 04:20
... real-time PCR and RTPCR offers significant advantages over standard PCR and RT-PCR for detection of DNA or RNA in terms of reduced sample handling, the time required for analysis and analytical ... elimination of carry-over contamination has led several manufacturers of commercial PCR and RT-PCR reagents to substitute dUTP in the reaction mixture in place of dTTP and in the case of some manufacturers ... 10 15 20 25 30 35 40 Cycle number 10 15 20 25 30 35 40 Cycle number Figure Simultaneous detection of viral RNA and contaminating DNA Simultaneous detection of viral RNA and contaminating DNA...
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Báo cáo khoa học: "Development of a Lightcycler-based reverse transcription polymerase chain reaction for the detection of foot-and-mouth disease virus" pps
Ngày tải lên :
07/08/2014, 18:21
... demrofrep erew noitceted tcudorp dna noitacifilpmA elcyc hcae rof ces/C 02 fo pmar eht htiw nim rof C 06 ta pets noisnetxe/gnilaenna dna ces rof C 49 ta pets noitarutaned fo selcyc 55 yb dewollof ... eht rof ylno detcelfer saw noiger B2 laitrap ot redaeL morf gnirevoc ,revewoh ,niarts 55/lizarb/oriezurc/42A rof ecneuqes ehT sniarts 21 eht rof dezylana saw noiger tegrat ehT )ASU ,ratsanD( ... elbaliava gnisu dengised erew stegrat lla rof seborp dna sremirp RCP-TR* emaN ecneuqeS eborP esreveR drawroF eborP esreveR drawroF eborP esreveR drawroF '3-pxGCCAATTTCAGCCCAGGCACAAAm-'5 '3-TYCGGTTYGGGACCATGTT-'5...
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Báo cáo khoa hoc:" Quantitative competitive reverse transcription polymerase chain reaction is not a useful method for quantification of CD4 and CD8 cell status during HIV infection" pptx
Ngày tải lên :
11/08/2014, 08:20
... between CD4 and CD8 mRNA levels and numbers of cells expressing these proteins on their surface The formation of CD4gp160 complexes in intracellular compartments may contribute to cell surface ... 0.5 U of Taq Polymerase For CD4 and CD8 reaction conditions were : 94° for mins, then 33 cycles of 94° for 45 secs, 55° for 45 secs, and 72° for mins on a Perkin Elmer Thermocycler 33 cycles ... CD4:CD8 cell ratios and are therefore likely to be HIV infected (the majority of the samples sent for flow cytometry are HIV-positive) The relationship between the mRNA and cell number for CD4 and...
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Báo cáo y học: " Variability in a dominant block to SIV early reverse transcription in rhesus monkey cells predicts in vivo viral replication and time to death" pot
Ngày tải lên :
12/08/2014, 04:20
... The ability of a cell to become infected by HIV/SIV requires cellular expression of diverse proteins and HIV/SIV replication can be repressed by the cellular expression of a number of restriction ... state of CD4+ T cells, is not entry dependent, does not reflect the susceptibility of a subpopulation of cells, is not dependent on multiple rounds of infection, is not specific for SIVsmE660 and ... between copy number /cell of each viral DNA species and the SIVsmE660 permissivity of that cell A significant positive correlation was observed between copy number of early RT, late RT, and integrated...
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Báo cáo y học: " A multiplex reverse transcription-nested polymerase chain reaction for detection and differentiation of wild-type and vaccine strains of canine distemper virus" pptx
Ngày tải lên :
12/08/2014, 04:20
... specific and sensitive assay for the rapid detection and differentiation of wildtype and vaccine strains of CDV For lack of other vaccine strains except CDV Onderstepoort strain, the primers P5 and ... products were cloned and sequenced, and the sequences were aligned with the H genes of a number of CDV strains available in GenBank using the MegAlign function of the DNAStar software package Thirty ... CAV, CCV, RV, NDV-infected cells, and uninfected cells by RT-nPCR (Fig 1) Sensitivity, applicability, and repeatability of the multiplex RT-nPCR Serial 10-fold dilutions of CDV vaccine strain were...
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Báo cáo y học: " The RNA binding protein HuR does not interact directly with HIV-1 reverse transcriptase and does not affect reverse transcription in vitro" doc
Ngày tải lên :
12/08/2014, 23:23
... characterization of GSTand NusA-HuR (A) Analysis of the expression and purification of GSTHuR by SDS PAGE Lanes and illustrate total protein and total soluble protein after E coli cell lysis, respectively, ... solution and the molecular masses of NusA-HuR and NusARRM were determined as 82.2 and 68.9 kDa, respectively These values are within ± 15% of the predicted masses of 99 kDa for NusA-HuR and 73 ... H domain of RT and to the C-terminus of HuR (see Fig 1) Importantly, siRNA knockdown of HuR expression in HeLa P4.2 cells was reported to greatly impair both the early and late steps of viral...
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Báo cáo y học: "Early and transient reverse transcription during primary deltaretroviral infection of sheep" ppt
Ngày tải lên :
13/08/2014, 06:20
... with many host cell pathways including cell cycle, apoptosis and DNA repair [2,3] This results in the persistent clonal expansion of infected cells and generates a significant level of genetic variability ... hundred and fifty copies of each of these cloned sequences were mixed in μg of uninfected DNA Five hundred nanograms of mixed DNA were amplified for 35 cycles using 200 μM of BLV3'S and BLV3'AS ... animals (p < 10-4 and Spearman's rho = 0.76) In animal 4535, the number of abundant clones increased during the course of the infection and the extensive proliferation of a subset of these clones...
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Báo cáo y học: " Contribution of the C-terminal tri-lysine regions of human immunodeficiency virus type 1 integrase for efficient reverse transcription and viral DNA nuclear import" pps
Ngày tải lên :
13/08/2014, 09:21
... integrated and unintegrated viral DNA [33] After 48 hours of infection, equal amounts of cells were lysed in 50 µl of luc lysis buffer and then, 10 µl of cell lysates was used for measurement of luc ... intracellular localization of YFP and IN-YFP by transfecting CMV-IN-YFP or CMV-YFP expressor in HeLa cells After 48 hours of transfection, cells were fixed and subjected to indirect immunofluorescence ... HeLa-CCR5-CD4-βGal cells, dividing and non-dividing C8166 T cells To compare the infection of each viral stock in HeLa-CCR5CD4-β-Gal cells, numbers of infected cells (β-Gal positive cells) were evaluated...
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Harvesting, oil extraction, and conversion of local filamentous algae growing in wastewater into biodiesel
Ngày tải lên :
05/09/2013, 15:28
... Healthcare, Bucks, England, UK) between the wavelengths of 400 and 700 nm in increments of 10 nm The peak absorption of light in the red portion of the spectrum was between 650 and 680 nm, and light at ... levels, and oil retrieved from the algal filaments represented between 3% and 6% of the initial dried weight Analysis of methanol extracts by TLC showed varying proportions of free fatty acids and ... lakes and rivers to the point of being “nuisance species” [8] Harvesting of these algae from shoreline areas of lakes (e.g., Lake Michigan, USA) could be an option for additional sources of biofuel...
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Nghiên cứu xây dựng quy trình phát hiện nhanh virus cúm gia cầm a h5n1 trong mẫu bệnh phẩm bằng kỹ thuật multiplex reverse transcription polymerase chain reaction
Ngày tải lên :
10/02/2014, 20:39
... “Glycosylation of haemagglutinin and stalk-length of neuraminidase combine to regulate the growth of avian influenza viruses in tissue culture” Virus Res, 79(1-2): 177-185 Beare AS and Webster RG ... Kida, H and Webster, RG (1996), “Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity ... “Establishment of multiple sublineages of H5N1 influenza virus in Asia: implications for pandemic control”, Proc Natl Acad Sci USA, 103, pp 2845- 50 15 Chen, H., G Deng, Z Li, G Tian, Y Li, and P Jiao...
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Tài liệu Báo cáo khoa học: Efficient RNA ligation by reverse-joined hairpin ribozymes and engineering of twin ribozymes consisting of conventional and reverse-joined hairpin ribozyme units ppt
Ngày tải lên :
20/02/2014, 01:20
... structure of the reverse-joined ribozyme unit for twin ribozyme design consists of two domains joined by a single-stranded linker of eight adenosine residues and binds the 3¢-terminal part of its ... Addition of the fragment S20 cp led to the formation of new products detected as three additional bands (lanes and 4) These signals correspond to the 29- and 35-mer resulting from ligation of the ... cp(Cy5) control Lanes and 3, incubation of S20 cp(Cy5) with HP–TWRJ in the absence of initial substrate under conditions of fragment exchange reaction after and 80 Lane 4, incubation of S20 cp(Cy5)...
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Tài liệu Báo cáo khoa học: "Extraction and Approximation of Numerical Attributes from the Web" pdf
Ngày tải lên :
20/02/2014, 04:20
... Usage of all this knowledge allows us to select the correct value of 1.695m and reject other values Thus we can increase the precision of value extraction by finding and analyzing an entire group of ... created a gold standard and compared against it automatically1 For WN enrichment evaluation, our framework discovered size and weight values for 300 WN physical objects, and the quality of results ... question answering (QA) evaluation, human-based WN enrichment evaluation, and human-based comparison of our results to data available through Wikipedia and to the top results of leading search engines...
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Tài liệu Báo cáo khoa học: "A Mobile Touchable Application for Online Topic Graph Extraction and Exploration of Web Content" ppt
Ngày tải lên :
20/02/2014, 05:20
... proceedings of the 5th FinTAL, Finland Eugenie Giesbrecht and Stefan Evert 2009 Part-ofspeech tagging - a solved task? An evaluation of PoS taggers for the Web as corpus In proceedings of the 5th ... types of word chains Each chain consists of longest matching sequences of words with the same PoS class, namely noun chains or verb chains, where an element of a noun chain belongs to one of the ... a topic graph T G = (V, E, A) consists of a set V of nodes, a set E of edges, and a set A of node actions Each node v ∈ V represents a chunk and is labeled with the corresponding PoS–tagged word...
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Tài liệu Báo cáo khoa học: "Integrating Information Extraction and Automatic Hyperlinking" docx
Ngày tải lên :
20/02/2014, 16:20
... French, Spanish, Chinese and Japanese allowing for named entity recognition and extraction To guarantee a comparable coverage, and to ease evaluation, an extension of the MUC-7 standard for entities ... rule Finally, the choice of feature structures as primary citizens of the information domain makes composition of modules very simple, since input and output are all of the same abstract data ... provides standard operations for constructing and manipulating TFSs; and (iv) an XTDL grammar interpreter Currently, SProUT offers three online components: a tokenizer, a gazetteer, and a morphological...
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Tài liệu Báo cáo khoa học: "A HARDWARE ALGORITHM FOR HIGH SPEED MORPHEME EXTRACTION AND ITS IMPLEMENTATION" pptx
Ngày tải lên :
21/02/2014, 20:20
... combinations of various candidates For example, " ~ ~", one of the extracted morphemes, is composed of the 2nd candidate at the 1st position, the 1st candidate at the 2nd position and the 3rd candidate ... number of all morphemes in the dictionary, L is the length of input text, M is the number of text streams, and A is the indexing coef~dent This coei~cient means the average rate for the number of ... M sets of character registers in the shift register block Each set is composed of N character registers, which store and shift the sub-string for one text strearn Here, M is the number of text...
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Building bone vitality: A Revolutionary Diet Plan to Prevent Bone Loss and Reverse Osteoporosis pdf
Ngày tải lên :
05/03/2014, 20:20
... diets, lifestyles, and thousands of other aspects of their lives Of course, it would be far too costly and time-consuming to follow hundreds of thousands or tens of millions of people for their entire ... rates of hip fracture According to the Food and Agriculture Organization of the United Nations, Americans and Western Europeans consume much more milk and dairy than Asians and Africans Think of ... vegetables, legumes, and soy products—with little, if any, meat, dairy, and fish and a modest amount of breads, cereals, and pastas For more than thirty years I have directed a series of clinical studies...
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Glycemic Load Diet Cookbook: 150 RECIPES TO HELP YOU LOSE WEIGHT AND REVERSE INSULIN RESISTANCE docx
Ngày tải lên :
06/03/2014, 05:20
... candies, including chocolate A serving of two one-inch squares of chocolate has a glycemic load of 68, considerably less than a slice of bread Look at it this way: a pile of candy the size of ... to the human diet, and we simply don’t have the mechanism to use big doses of it gradually When you eat a big dose of starch—say, a plate of spaghetti and a couple of slices of garlic bread—it ... on our cells, the insulin receptors, get harder and harder to open— think of them as having rusty hinges It takes more and more and more insulin to open the doors and get the sugar out of our...
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