production of supported catalysts by impregnation and viscous drying

production of recombinant proteins by microbes and higher organisms demain 2009

production of recombinant proteins by microbes and higher organisms demain 2009

... Cohen and Boyer in California This was followed years later by the start of Genentech, and then by other corporations such as Amgen and Biogen, etc By 2002, over 155 approved pharmaceuticals and ... to excessive growth rates and aeration Production of extracellular human alpha interferon by B subtilis is repressed by high growth rate and by excess oxygen (Meyer and Fiechter, 1985) An exoprotease-deficient ... consistent performance, production of a bioactive product, and lack of contamination by viruses and DNA Clinical trials and product approval requires at least 4–5 years at a cost of 60 to 100 million...

Ngày tải lên: 08/09/2015, 22:11

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Production of recombinant proteins by microbes and higher organisms demain 2009

Production of recombinant proteins by microbes and higher organisms demain 2009

... Cohen and Boyer in California This was followed years later by the start of Genentech, and then by other corporations such as Amgen and Biogen, etc By 2002, over 155 approved pharmaceuticals and ... to excessive growth rates and aeration Production of extracellular human alpha interferon by B subtilis is repressed by high growth rate and by excess oxygen (Meyer and Fiechter, 1985) An exoprotease-deficient ... consistent performance, production of a bioactive product, and lack of contamination by viruses and DNA Clinical trials and 303 product approval requires at least 4–5 years at a cost of 60 to 100 million...

Ngày tải lên: 09/09/2015, 12:14

10 740 0
Production of recombinant proteins by microbes and higher organisms

Production of recombinant proteins by microbes and higher organisms

... performance, production of a bioactive product, and lack of contamination by viruses and DNA Clinical trials and validate and to 303 product approval requires at least 4-5 years at a cost of 60 to 100 million ... produces 10,000 L of milk per year compared to 6000 L of urine One of the negative points in production of proteins by transgenic animals is the length of time needed to assess production level ... weeks and the cell culture is sustainable for only a limited time Overall, 39% of recombinant proteins are made by E coli, 35% by CHO cells, 15% by yeasts, 10% by other mammalian systems and 1% by...

Ngày tải lên: 09/09/2015, 12:34

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Báo cáo y học: "The new IL-1 family member IL-1F8 stimulates production of inflammatory mediators by synovial fibroblasts and articular chondrocytes" ppt

Báo cáo y học: "The new IL-1 family member IL-1F8 stimulates production of inflammatory mediators by synovial fibroblasts and articular chondrocytes" ppt

... IL-1β Shown is an analysis of the effects of IL-1F8 and IL-1β on IL-1β production of (a) IL-6 and (c) IL-8 by hSFs, and of (b) IL-6, (d) IL-8 and (e) nitric oxide by hACs Cells were treated with ... sim- Page of 11 (page number not for citation purposes) Arthritis Research & Therapy Vol No Magne et al Figure Production of IL-6, IL-8 and nitric oxide by hSFs and hACs: effects of IL-1F8 and IL-1β ... course of induction of endogenous IL-1β mRNA by IL-1β in hSFs In hACs expression of IL-1F8 mRNA was constitutive and IL-1F8 levels were not affected by stimulation of cells with IL-1β and TNF-α...

Ngày tải lên: 09/08/2014, 08:22

11 325 0
Comparative decolorizing efficiency of textile dye by mesophilic and thermophilic anaerobic treatments

Comparative decolorizing efficiency of textile dye by mesophilic and thermophilic anaerobic treatments

... anaerobic mesophilic and thermophilic conditions, the variation of MO at concentration of 100, 200, 400, 600, 800 and 1,000mg·L-1 and RB4 at concentration of 100, 200, 300, 400, 500 and 600mg·L-1 supplemented ... digested sludge The potential of digested sludge on MO and RB4 tested by variation of dye concentration was shown by the different extent of decolorizing efficiency of the tested dyes MO could ... increase and presence of dye was associated with inhibition of organic matters conversion pathways caused by of the accumulation of volatile fatty acids in the treatment system In the case of temperature...

Ngày tải lên: 05/09/2013, 09:38

10 405 0
Control of Membrane Fouling by Coagulant and Coagulant Aid Addition in Membrane Bioreactor Systems

Control of Membrane Fouling by Coagulant and Coagulant Aid Addition in Membrane Bioreactor Systems

... mixture of polysilicate and Fe(III) was used in this study Behaviors of these materials on biopolymer (protein and carbohydrate) removal and fouling reduction were investigated by conducting batch and ... L/day with 1,000 mg/L of biological oxygen demand (BOD), 50 mg/L of nitrogen and 25 mg/L of phosphorus The experiments were conducted at a sludge retention time (SRT) of 33 days and the hydraulic ... and Mishima, 2005) For the SMP

Ngày tải lên: 05/09/2013, 10:15

11 575 0
A White Paper Describing Produced Water from Production of Crude Oil, Natural Gas, and Coal Bed Methane pot

A White Paper Describing Produced Water from Production of Crude Oil, Natural Gas, and Coal Bed Methane pot

... approximately 98% of the total volume of exploration and production (E&P) waste generated by the oil and gas industry and is the largest volume waste stream generated by the oil and gas industry ... volume of produced water and other wastes generated as a result of oil and gas E&P in the U.S and how those wastes were managed and disposed of Due to the differences between onshore and offshore ... development, and production of oil and gas on federal lands In addition, the BLM approves and supervises most oil and gas operations on American Indian lands The BLM regulations governing onshore oil and...

Ngày tải lên: 09/03/2014, 01:20

87 559 0
Báo cáo khóa học: Differential carbohydrate epitope recognition of globotriaosyl ceramide by verotoxins and a monoclonal antibody pdf

Báo cáo khóa học: Differential carbohydrate epitope recognition of globotriaosyl ceramide by verotoxins and a monoclonal antibody pdf

... externally calibrated with the molecular mass of a mixture of standard peptides Results Comparison of ligand/Gb3 binding by RELISA The binding of VT1, VT2, and mAb anti-Gb3 (38.13) to Gb3 was assessed ... more distinct and discriminatory at 37 °C (and at room temperature) compared with °C Type B glomerular and tubular Gb3 are similar and bound by VT1 Fig Comparison of VT1, mAb anti-Gb3 and VT2 binding ... monodeoxyGb3 analogues by TLC overlay DeoxyGb3 analogues were separated by TLC and visualized by (A) orcinol chemical detection Immunodetection of ligand binding was detected by TLC overlay for (B)...

Ngày tải lên: 16/03/2014, 16:20

13 398 0
Báo cáo khóa học: Mechanistic insight into the peroxidase catalyzed nitration of tyrosine derivatives by nitrite and hydrogen peroxide doc

Báo cáo khóa học: Mechanistic insight into the peroxidase catalyzed nitration of tyrosine derivatives by nitrite and hydrogen peroxide doc

... 35100% of buffer B in 20 min, and monitored at 280 and 412 nm The lactoperoxidase containing peak was manually collected and the homogeneity of the protein was checked by SDS/PAGE under reducing and ... analyzed by HPLC as described above The retention times of and were 11.5 and 12.7 min, respectively, and those of the corresponding nitration products, 3-(4-hydroxy-3-nitrophenyl)-propionic acid and ... peaks at 11.1 and 11.3 min, and minor peaks for unreacted and six other products with retention times of 9.9, 10.4, 11.8, 12.0, 12.2 and 12.4 Fig Biphasic behavior of the rate of HRP-mediated...

Ngày tải lên: 16/03/2014, 16:20

12 414 0
Báo cáo khoa học: Perturbation of folding and reassociation of lactate dehydrogenase by proline and trimethylamine oxide potx

Báo cáo khoa học: Perturbation of folding and reassociation of lactate dehydrogenase by proline and trimethylamine oxide potx

... level of inhibition was 3.2 M Perturbation of the reactivation of acid-dissociated LDH by proline and TMAO Reactivation of acid-dissociated BHLDH was inhibited by proline Both the initial rate and ... interactions of sidechains and the polypeptide backbone with osmolytes were provided in a recent review by Bolen and Baskakov [5] Analysis of the free energy of transfer of the sidechains and polypeptide ... the effects of proline and TMAO on the structure and/ or function of lactate dehydrogenase are based on investigations performed by Bolen and coworkers [3,44,45] A study of the effect of proline...

Ngày tải lên: 23/03/2014, 15:21

12 474 1
TOWARDS A COMPREHENSIVE APPROACH OF SEXUAL AND REPRODUCTIVE RIGHTS AND NEEDS OF WOMEN DISPLACED BY WAR AND ARMED CONFLICT pptx

TOWARDS A COMPREHENSIVE APPROACH OF SEXUAL AND REPRODUCTIVE RIGHTS AND NEEDS OF WOMEN DISPLACED BY WAR AND ARMED CONFLICT pptx

... status of women, especially pregnant, and of infants and children By 2005: 60% of PHC and FP facilities should offer prevention and management of reproductive tract infections 33 Prevalence of anaemia ... week of pregnancy but before delivery of the baby Assessment: The process of identifying and understanding a problem and planning a series of actions to deal with it There are usually a number of ... understanding of the impact of conflict on the SRH rights and needs of women displaced by war and armed conflict To assure a multidisciplinary, multisectoral and integrated approach of women’ s sexual and...

Ngày tải lên: 28/03/2014, 14:20

41 470 0
A WORLD WIDE REVIEW OF THE COMMERCIAL PRODUCTION OF BIODIESEL – A technological, economic and ecological investigation based on case studies pot

A WORLD WIDE REVIEW OF THE COMMERCIAL PRODUCTION OF BIODIESEL – A technological, economic and ecological investigation based on case studies pot

... Fuel storage and distribution Fuel production Feedstock transport Feedstock and fertilizer production Land use changes and cultivation CH4 and CO2 leaks and flares Emissions displaced by co-products ... Fuel storage and distribution Fuel production Feedstock transport Feedstock and fertilizer production Land use changes and cultivation CH4 and CO2 leaks and flares Emissions displaced by co-products ... Fuel dispensing Fuel storage and distribution Fuel production Feedstock transport Feedstock and fertilizer production Land use changes and cultivation CH4 and CO2 leaks and flares C in end-use fuel...

Ngày tải lên: 29/03/2014, 17:20

164 601 3
Báo cáo khoa học: The thermodynamic analysis of protein stabilization by sucrose and glycerol against pressure-induced unfolding The typical example of the 33-kDa protein from spinach photosystem II docx

Báo cáo khoa học: The thermodynamic analysis of protein stabilization by sucrose and glycerol against pressure-induced unfolding The typical example of the 33-kDa protein from spinach photosystem II docx

... Plot of the standard volume change of the 33-kDa protein in the presence of different concentration of NaCl (d) and sucrose (m) vs the reciprocal of the sucrose osmolarity In Fig 8, the standard ... Kinetics of the pressure-induced unfolding and refolding of the 33-kDa protein in the presence of 30% glycerol Other conditions as in Fig Table Relaxation time of unfolding and refolding of the ... denaturation Effects of sucrose and glycerol on the kinetics of the pressure induced protein unfolding and refolding The unfolding and refolding kinetics in the presence of either 0.83 M sucrose...

Ngày tải lên: 31/03/2014, 01:20

8 527 0
Báo cáo hóa học: " Genetic analysis of hantaviruses carried by Myodes and Microtus rodents in Buryatia" doc

Báo cáo hóa học: " Genetic analysis of hantaviruses carried by Myodes and Microtus rodents in Buryatia" doc

... supported by grants from the University of Helsinki, The Academy of Finland, Sigrid Jusélius foundation (Finland) and EU grant GOCE-CT-2003-010284 EDEN and the paper is officially catalogued by the ... sequences of the N protein of PUUV, HOKV, and MUJV were of the same length (433 aa residues), a clear sign of their close genetic ties and shared evolution Furthermore, the N protein sequence of Muhorshibir767 ... filter paper to PBS and were screened by immunofluorescence assay (IFA) for the presence of antibodies to hantaviruses (Puumala and Dobrava virus antigens) (the details of trapping and IFA-screening...

Ngày tải lên: 20/06/2014, 01:20

6 442 0
The Production of Ethanol from Maize Cobs and Groundnut Shells docx

The Production of Ethanol from Maize Cobs and Groundnut Shells docx

... 4.5M and 5M of H2SO4 and the various acid concentrations recorded Using the best concentration of H2SO4 (4.5M), a mixture of maize cobs and groundnut shells in the ratio of 1:1, 1:2, 2:1, 1:3 and ... amount of groundnut shells with structures, which contain high degree of crystallinity and polymerization thereby, limit accessibility to acid attack Ratio 3:1 of maize cobs and groundnut shells and ... high quality of ethanol To determine the effect of different acid concentrations on the hydrolysis 2M solution of concentrated H2SO4 was prepared and 10g of the leached maize cobs and groundnut...

Ngày tải lên: 06/07/2014, 18:20

5 356 0
Báo cáo toán học: "Dihedral F-Tilings of the Sphere by Equilateral and Scalene Triangles - II" pptx

Báo cáo toán học: "Dihedral F-Tilings of the Sphere by Equilateral and Scalene Triangles - II" pptx

... composed by congruent cells, and dihedral if every tile of τ is congruent to one of two fixed sets X and Y (prototiles of τ ) We shall denote by Ω(X, Y ) the set, up to isomorphism, of all dihedral ... configuration and 3D representation of D For each p ≥ 4, we obtain a global configuration of a tiling τ ∈ Ω(T1 , T2 ) with vertices of valency 4, and 2p composed by 4p equilateral and 4p scalene ... obtain an f-tiling E m that has one class of vertices of valency 4, one of valency 8, and one of valency 2m, being composed of 4m equilateral triangles and 8m scalene triangles A 3D representation...

Ngày tải lên: 07/08/2014, 21:20

21 259 0
Báo cáo toán học: "Dihedral f-Tilings of the Sphere by Equilateral and Scalene Triangles - III" potx

Báo cáo toán học: "Dihedral f-Tilings of the Sphere by Equilateral and Scalene Triangles - III" potx

... representation of a tiling τα ∈ Ω(T1 , T2 ) as is shown in Figure This family of tilings is composed by two equilateral and six scalene triangles and is denoted by Eα Figure 4: 2D and 3D representation of ... isohedrality-classes and isogonality-classes for the symmetry group are denoted, respectively, by # isoh and # isog.; • By Cn and Dn we denote, respectively, the cyclic group of order n and the dihedral group of ... decompositions of the sphere by geodesic polygons, such that all vertices are of even valency, the sums of alternate angles around each vertex are π and every tile is congruent to one of two fixed sets X and...

Ngày tải lên: 07/08/2014, 21:21

34 230 0
Báo cáo y học: "Human rheumatoid arthritis tissue production of IL-17A drives matrix and cartilage degradation: synergy with tumour necrosis factor-α, Oncostatin M and response to biologic therapies" ppt

Báo cáo y học: "Human rheumatoid arthritis tissue production of IL-17A drives matrix and cartilage degradation: synergy with tumour necrosis factor-α, Oncostatin M and response to biologic therapies" ppt

... cohort and participated in study design and data analysis JMcC, MC and OS performed some experiments BB and OF participated in study design and data analysis UF and DV conceived the study and developed ... by IL-17A (10 and 20 ng/ml) and 4.4 fold by TNF-α from basal Page of 12 (page number not for citation purposes) of 9202.64 ± 8806.31 ng/mg of tissue to 16,711.80 ± 15,535.07 ng/mg of tissue (P ... baseline assessment of disease activity Patients were evaluated before and at 1, and 12 months after initiation of biologic therapy Blood samples were obtained and sera was separated and stored at...

Ngày tải lên: 09/08/2014, 14:22

12 262 0
Báo cáo y học: " Increased production of viral proteins by a 3''''-LTR-deleted infectious clone of human T-cell leukemia virus type 1" ppsx

Báo cáo y học: " Increased production of viral proteins by a 3''''-LTR-deleted infectious clone of human T-cell leukemia virus type 1" ppsx

... chromosome 14 DNA; 1256 bp) of the Δ3' LTR, and removed 1169 bp from the Δ3' LTR The reconstructed clone had 39 bp of the 3' LTR and 87 bp of human chromosome 14 DNA, and was designated Δ3' LTR ... with deletions of the 3' LTR of different lengths and the corresponding viral expression patterns Various Various molecular clones with deletions of the 3' LTR of different lengths and the corresponding ... expression patterns (A) Diagram of the molecular clones showing the deletion series of the 3' LTR and the deletion of the reverse TATA box of the 3' LTR (B) The amount of p19 gag in the pooled concentrated...

Ngày tải lên: 12/08/2014, 04:21

5 261 0
Báo cáo khoa học: "A comparison of decision-making by physicians and administrators in healthcare settings" ppt

Báo cáo khoa học: "A comparison of decision-making by physicians and administrators in healthcare settings" ppt

... of the fairness of the organization [5] This may be particularly relevant in situations where budget constraints are severe In a model of instructors and students, the use of the principles of ... little analysis of the current status of its principles or the impact of implementing them [8] In the example given, the principles of justice are relevant and applicable Appreciation and clear articulation ... ‘Directoring’ and managing in a professional system Modern Pathol 1998, 11:585-592 Jane Jacobs: Systems of Survival: A Dialogue on the Moral Foundations of Commerce and Politics New York: Random House;...

Ngày tải lên: 13/08/2014, 03:20

3 278 1
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