principles procedures and evidence base
Báo cáo sinh học: " Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections" doc
... participated in design and coordination of the study and helped to draft the manuscript All authors read and approved the final manuscript Material and Methods Virus culture and virus detection ... http://www.virologyj.com/content/2/1/7 The respective MOI and time of cell culture are shown in table and were chosen to allow maximal infection as determined by immunofluorescence and real-time PCR [8-11] For kinetic ... viral RNA was performed to proof and monitor infection Thereafter the candidate reference genes were evaluated by the BestKeeper tool [6], the GeNorm tool [7] and the algorithm we described previously...
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báo cáo hóa học:" Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections" doc
... participated in design and coordination of the study and helped to draft the manuscript All authors read and approved the final manuscript Material and Methods Virus culture and virus detection ... http://www.virologyj.com/content/2/1/7 The respective MOI and time of cell culture are shown in table and were chosen to allow maximal infection as determined by immunofluorescence and real-time PCR [8-11] For kinetic ... viral RNA was performed to proof and monitor infection Thereafter the candidate reference genes were evaluated by the BestKeeper tool [6], the GeNorm tool [7] and the algorithm we described previously...
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Báo cáo y học: " Phosphodiesterase type 4 expression and anti-proliferative effects in human pulmonary artery smooth muscle cells" docx
... absence and presence of drugs at specified concentrations The medium was collected after 48 h and gelatinase (MMP-2 and MMP-9) activity visualized by zymography and measured using MMP-2 and MMP-9 ... lung and vasculature [11,12] PDE4 proteins are encoded by four genes (PDE4A, PDE4B, PDE4C and PDE4D), which produce numerous PDE4 variants [10,13] and studies on rat pulmonary arteries [14] and ... significant Results PDE4 gene expression and activity Products of 546, 506, 410 and 479 base pairs (bp), corresponding to fragments of PDE4A, PDE4B, PDE4C and PDE4D respectively were amplified by...
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Báo cáo y học: " Ancient, independent evolution and distinct molecular features of the novel human T-lymphotropic virus type 4" pot
... p30II, and p13II (where I and II denote ORFI and ORFII, respectively), have been identified in the pX region of HTLV-1 and are important in viral infectivity and replication, T-cell activation, and ... and was specific based upon banding patterns observed in the presence of specific and non-specific oligonucleotide competitors in an electrophoretic mobility shift assay (EMSA) The shifted band ... donor site (sd-LTR), and two 21-bp repeat elements (middle and proximal based on positions in HTLV-1 and -2), as well as the location of the distal 21-bp repeat in HTLV-1 and -2 (dashed lines),...
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A comparative study of discourse structures and some major linguistic features of international declarations and international conventions on human rights part 4
... right to a standard of living adequate for the health and well-being of himself and of his family, including food, clothing, housing and medical care and necessary social services, and the right ... security and is entitled to realization, through national effort and international co-operation and in accordance with the organization and resources of each State, of the economic, social and cultural ... personality and to the strengthening of respect for human rights and fundamental freedoms It shall promote understanding, tolerance and friendship among all nations, racial or religious groups, and shall...
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Tài liệu E-Human Resource Management 4 pptx
... understand the complexities of a new situation Otherwise they will be slow to process and retain new facts and concepts because they will lack an ability to interpret and classify information based ... the financial and human resources required, the organizational structures, and the major systems and procedures These are the building blocks that can be redesigned, added to, and reconfigured ... strategy and sharing of common values, communication and feedback processes, levels of trust and perceived organizational justice, employee commitment, and organizational readiness and flexibility...
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Báo cáo khoa học: The Saccharomyces cerevisiae orthologue of the human protein phosphatase 4 core regulatory subunit R2 confers resistance to the anticancer drug cisplatin pot
... supernatant and pellet fractions were analysed by SDS ⁄ PAGE and immunoblotting with MYC antibodies (A) YBL046w–MYC13 and HA3– Pph3p (B) YBL046w–MYC13 and HA3–Sit4p (C) YBL046w–MYC13 and HA3–Pph21p ... extract, 2% peptone and 2% glucose Synthetic complete medium (SC) contained 0.67% yeast nitrogen base (Difco Laboratories, Detroit, MI, USA), 2% glucose and amino acids and bases as described ... concentrations of benomyl and grown at 26 °C for days of strains carrying deletions of PPH3, YBL046w ⁄ PSY4 and PSY2 Experimental procedures Yeast strains, plasmids, media and general methods Saccharomyces...
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Báo cáo khoa học: Mutational analysis of substrate recognition by human arginase type I ) agmatinase activity of the N130D variant pot
... °C and pH 7.5), there was no significant difference in the thermal stability of the wild-type and mutant enzymes; and (c) the ion exchange chromatographic properties on a DEAE–cellulose column and ... variant On the other hand, as a potential ligand for a primary amino group, Asp130 would contribute to the increased affinity of the mutant enzyme for agmatine and putrescine and, thus, to its ability ... Arginase: structure, mechanism, and physiological role in male and female sexual arousal Acc Chem Res 38, 191–201 10 Satishchandran C & Boyle SM (1986) Purification and properties of agmatine ureohydrolyase,...
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Báo cáo khoa học: Effect of heliquinomycin on the activity of human minichromosome maintenance 4/6/7 helicase pdf
... MCM4/6/7 complex (120 ng) and SV40 T antigen (200 ng) in the presence of single-stranded DNA The radioactivity at the sites to which Pi and ATP migrated was measured, and the ratio of the released ... exclusion and actively translocate along a duplex J Biol Chem 278, 49171–49182 Bochman M & Schwacha A (2007) Differences in the single-stranded DNA binding activities of MCM2-7 and MCM467: MCM2 and ... the MCM4/6/7 complex, SV40 T antigen and Werner protein, on the oligonucleotide displacement activity of replication protein A (RPA), and on the RNA priming and DNA polymerization activities of...
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Báo cáo khoa học: Functional analysis of disease-causing mutations in human UDP-galactose 4-epimerase pot
... [27], and thus the observed activity probably reflects that of the native enzyme With the exception of L183P, all the mutant proteins could be expressed and purified using similar conditions and procedures ... Experimental procedures Expression and purification of human GALE Human GALE was expressed in and purified from E coli, essentially as described previously for galactose mutarotase and galactokinase ... L313M, G319E and R335H) are associated with peripheral forms of the disease In this study, all nine disease-causing mutations have been expressed in, and purified from, Escherichia coli, and their...
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Báo cáo sinh học: " Purification of infectious human herpesvirus 6A virions and association of host cell proteins" pot
... contributions MH and JA carried out the purification and analysis of the study with equal contribution and drafted the manuscript AF-H, SJ and HG participated in its design and coordination and helped ... 3, and dpi are shown Results are based on three experiments and presented as mean ± standard deviation (error bars) B and C Light microscopic analyses of HHV-6A- and mock-infected JJHAN cells at ... in HHV-6A infected cells D and E Detection of HHV-6 antigen gp60/110 in HHV-6A infected cells HHV-6A -and mockinfected cells were fixed and stained for gp60/110 (red) and counterstained with DAPI...
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Báo cáo sinh học: " The simultaneous presence and expression of human hepatitis C virus (HCV), human herpesvirus-6 (HHV-6), and human immunodeficiency virus-1 (HIV-1) in a single human T-cell" docx
... body and CIMM Authors' contributions K.A.S., A.G., and R.G performed biological work J.G.P and A.S.K performed the clinical work, recruitment of patients, and procurement of specimens K.A.S and ... fixation and processing Figure gels of PCR products Agarose3 Agarose gels of PCR products The DNA standard is a kb plus ladder (Invitrogen) Arrows indicate the expected band sizes Positive bands ... K3, and Lane is K4 The positive bands are 650 base pairs in size F HIV-1 Lane is a 100 bp ladder (Invitrogen) Lane is K6 and Lane is K7 HIV-1 infected cells and finally the HCV infected ones...
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Báo cáo sinh học: " Thiol-reactive reagents inhibits intracellular trafficking of human papillomavirus type 16 pseudovirions by binding to cysteine residues of major capsid protein L" pot
... the DE-loop and C225 and C229 are involved in forming the EF-loop Because the loops are generally flexible and because C146 and C225 are located at the surface region of the loops and C229 is ... (lanes and 8) and became resistant after the incubation at 37°C for h (lanes and 9) The cells inoculated with the 16PVs were further incubated with the growth medium at 37°C for 2, 4, 8, and 20 ... free thiols The DTNB-16PVs and the NEM-16PVs, whose C146, C225, and C229 carried DTNB and NEM as additional side chains, respectively, bound to HeLa cells less efficiently and were degraded rapidly...
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Báo cáo sinh học: " The E5 protein of the human papillomavirus type 16 down-regulates HLA-I surface expression in calnexin-expressing but not in calnexin-deficient c" pptx
... between the oncogenes HPV16 E5, E6 and E7 and different apoptotic pathways have already been identified [9] E6 and E7 modulate apoptosis by binding and inactivating p53 and the product of tumour suppressor ... CEM-C7 and CEM-NKR cell lines were electroporated using 1×107 cells in 200 µl PBS, 10 µg DNA and setting the pulser to 220 Volt and 960 µFarad (Bio-Rad GenePulser) Transfected CEM-C7 and CEM-NKR ... probabilities for the sequences of E5 and the mutants M1, M2 and M3 Transmembrane Hidden Markov Model posterior probabilities for the sequences of E5 and the mutants M1, M2 and M3 A) Amino acid sequence...
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Báo cáo sinh học: " Human herpesvirus 6A induces apoptosis of primary human fetal astrocytes via both caspase-dependent and -independent pathways" potx
... and WXH designed and directed studies, and were involved in the interpretation of the data and writing of manuscript BG, GFZ and FZ carried out the cells culture and HHV-6 infection BG, JFW and ... assay and the western blot analysis BG, DJF and CZ performed the flow-cytometry experiments BG, JC and DDG performed the analysis of Caspase-8 and -9 activities and electron microscopy CLD and ... dehydrated in a series of 70%, 80% and 90% ethanol, and embedded in Epon Ultrathin sections were cut and mounted in nickel grids, stained with uranyl acetate and lead citrate, and examined by a transmission...
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Báo cáo sinh học: " Human herpesvirus 8 – A novel human pathogen" potx
... virus isolates These repeats are 801 base pairs in length with 85% G/C content, and have putative packaging and cleavage signals [19] The LUR is similar to HVS and the HHV-8 genes are named after ... [50-52,54,55] and another five [5052,55,56] compared HIV+ and HIV- subjects for the presence of HHV-8 Grandadam et al [53] investigated multicentric Castleman's disease (MCD) in HIV+ patients and Luppi ... are no large studies of the donor's and the recipient's HHV-8 serostatus and presence of HHV-8 in donor blood and organ Properly done, both antibody prevalence and a determination of infectious...
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nghiên cứu phát hiện sự gắn chèn gene e6 và e7 của human papilomavirus type 16 vào bộ gene người bằng kỹ thuật multiplex rt-pcr
... ph i BLAST ngư i Basic Local Alignment Search Tool: Công c tìm ki m trình t tương ng Bp base pairs: C p base CIN Cervical Intraepithelial Neoplasia: T n thương t bào bi u mô c t cung Cs C ng s ... tài li u v thi t k m i c a Andrew Wallace (1996), Alkami Biosystems (1999) , m t s tiêu chu n c a m i c rút sau: - Chi u dài c a m i t t nh t n m kho ng 18 – 25 c p base - Thành ph n nucleotide ... cân b ng Các m i có hi u qu khu ch i t t nh t nh ng m i có base lo i G, C t p trung thành nhóm chi m t l kho ng t 40% - 60% Ngoài ra, base cu i u 3’hay g n u 3’ c a m i nên G, C, GC ho c CG -...
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Nuclear Power Control, Reliability and Human Factors Part 4 docx
... Nuclear Power Systems 107 system-level information and commands, and spacecraft-level status and demands The effective integration of data and information at each level requires a well-defined ... operation regarding the compliance with national and international standards, recommendations, regulatory guides, reliability and availability metrics, and security mechanisms, were established Within ... (1999c) IEEE Standard 802.11a, Part 11: Wireless LAN Medium Access Control (MAC) and Physical Layer (PHY) Specifications High Speed Physical Layer in the GHz Band IEEE (2003) IEEE Standard 802.11g,...
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Báo cáo hóa học: " Purification of infectious human herpesvirus 6A virions and association of host cell proteins" potx
... contributions MH and JA carried out the purification and analysis of the study with equal contribution and drafted the manuscript AF-H, SJ and HG participated in its design and coordination and helped ... 3, and dpi are shown Results are based on three experiments and presented as mean ± standard deviation (error bars) B and C Light microscopic analyses of HHV-6A- and mock-infected JJHAN cells at ... in HHV-6A infected cells D and E Detection of HHV-6 antigen gp60/110 in HHV-6A infected cells HHV-6A -and mockinfected cells were fixed and stained for gp60/110 (red) and counterstained with DAPI...
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