prevention of its genetic damage by means of chemical agents

... importance of validating your antibody One of the major difficulties of immunological detection of proteins of interest is nonspecific binding of the antibody, which causes misinterpretation of the ... greatest sources of autofluorescence discussed here are those induced by aldehyde fixation and by the fluorescent pigment lipofuscin, which accumulates with age in the cytoplasm of cells of the central ... obtain such genetically-encoded fluorescent labeled proteins, the cDNA of the fluorescent label and that of the protein of interest have to be cloned in frame with each other The use of genetically-encoded...

Ngày tải lên: 28/06/2014, 08:20

... number of cells in mitosis In addition, in MS experiments, absence of a condition is not proof of its nonexistence Whereas, initially, it was found that PTMs in the N-terminal tail of most of the ... and dosage, it did establish, by the use of antibodies, that the level of phosphorylated Thr18 in the N-terminal tail of H1.4 was reduced by treatment with any one of these three compounds In the ... of the linker histones Lysine and N-terminus acetylations Acetylation of the Na-terminus involves the cotranslational cleavage of a methionine, followed by acetylation of the second residue of...

Ngày tải lên: 18/02/2014, 08:20

... al Journal of Nanobiotechnology 2010, 8:25 http://www.jnanobiotechnology.com/content/8/1/25 Page of 14 Figure Characterization of IRDy800CW-MNPs (A) TEM image of MNPs (B) TEM image of IRDy800CW-MNPs ... et al Journal of Nanobiotechnology 2010, 8:25 http://www.jnanobiotechnology.com/content/8/1/25 Page of 14 Figure Measurement of the relative iron-loading of cells (A) NIRF signal of cells labeled ... different doses of the Hou et al Journal of Nanobiotechnology 2010, 8:25 http://www.jnanobiotechnology.com/content/8/1/25 Page of 14 Figure Measurement of cell viability (A) NIRF signals of cells treated...

Ngày tải lên: 11/08/2014, 00:22

... advantage of the self-splicing ability of genetically modified inteins coupled with a unique chemical reaction described in the following section 1.2 Native chemical ligation Covalent chemical ... final volume of 150 µl At specific time intervals, 15 µl of the reaction was withdrawn and quenched by addition of 1.7 µl of 100 mM cysteine to the reaction mixture (final concentration of cysteine: ... entirely by the amino acid residues contained in the intein.[1] The biochemical mechanism of protein splicing includes the formation of a (thio)ester intermediate and the final step of a N→O...

Ngày tải lên: 08/11/2015, 16:30

... 1997, the primary classification of carbohydrates should be based on chemistry; that is, by molecular size, degree of polymerization, the type of linkage and character of individual monomers This ... ―Carbohydrate Intake and Prevention of Nutrition-Related Disease‖ published in 2011, that for primary prevention purposes the quality of carbohydrate nutrition is more important than its quantity [23] ... for its glycemic potency and is defined as the amount of available carbohydrates multiplied by their respective GI In epidemiological studies, not the GI or GL values of a single food are of interest...

Ngày tải lên: 25/11/2015, 14:52

... model (GLM) procedure of SAS software (SAS 1989) in order to identify significant sources of variation The least-squares means were compared using Tukey HSD tests from each genetic group The sperm ... meat percentage 3.2 Semen quality 3.2.1 Influence of genetic group on semen quality The table shows that semen quality traits are influenced by genetic background (P

Ngày tải lên: 28/08/2013, 11:59

... cultivation and the ratio of the increased amount of SS to the increased amount of Chl.a was higher than that of the algae Increased amount of SS were higher in the cases of high nitrate concentration ... more of the potential of the denitrifying activity was exploited The dissolution rate of organic matter from the sediment was sufficient, and limitation of denitrification caused by depletion of ... During the 573 days of observation, 7800 kg-N of nitrogen flowed in, 10% of nitrogen was denitrified biologically, and 40% of it was removed by algal activity Almost 50% of nitrogen out-flowed...

Ngày tải lên: 05/09/2013, 10:17

... scientific studies of hypnosis by scientists such as Clark L H [10] led to the extension of the meaning of these words in a special and technical sense The original neuro-psychological theory of hypnotic ... description of the syntactic and semantic features of SVs in English and their Vietnamese equivalents It is hoped that the results of the study can help to raise the awareness of Vietnamese learners of ... of the Verb 2.2.2 Definition of Verb Phrase 2.2.3 Verb Classification 2.2.4 Syntactic Features of Verbs 2.2.4.1 Syntactic Relations 2.2.4.2 Grammatical Categories of Verbs 2.2.4.3 Functions of...

Ngày tải lên: 26/11/2013, 13:17

... University of Danang Time : 27/6/2012 Venue : University of Danang * The thesis is accessible for the purpose of reference at: - Library of the College of Foreign Languages, University of Danang ... Adverbial Clauses of Time + Adverbial Clauses of Place + Adverbial Clauses of Condition + Adverbial Clauses of Concession + Adverbial Clauses of Reason + Adverbial Clauses of Comparison ■ Relative ... the smaller units of languages” He refers to the following methods of translation 2.2.1.2 Translation Procedures As above mentioned, various types of equivalence occur on all levels of language,...

Ngày tải lên: 26/11/2013, 13:21

... total debt at 60 % of its GDP As a proxy we use a country’s total outstanding bonds relative to its GDP This choice of variable is motivated by data availability as the amount of bonds outstanding ... these general issues many of which are related to US subprime assets is beyond the scope of this paper (Stulz, 2010 offers a comprehensive review of the risks and benefits of the CDS market) Our ... risk-free rates, a core feature of asset pricing The purpose of this paper is to provide a comprehensive analysis of the Euro area sovereign CDS market by making use of information from the underlying...

Ngày tải lên: 16/02/2014, 02:20

... interaction of Yef1p with Utr1p is of biological interest and may be related to the pronounced requirement of both Yef1p and Utr1p in the absence of Pos5p The elucidation of the localization of Utr1p ... amount of enzyme was incubated at 30 °C Enzyme solution of less than 100 lL was routinely added to the reaction mixture The reaction was terminated by the addition of 0.1 mL of m NaOH followed by ... abilities of LEU2, UTR1 and YEF1 for the growth defects of the pos5 mutant To confirm the complementing ability of LEU2 for the growth defects of the pos5 mutant, we examined the growth of pos5...

Ngày tải lên: 20/02/2014, 01:20

... units of the N-linked chains from each of the N-glycosylation sites of bovine ZPA The values in parentheses are glucose units of authentic pyridylaminated carbohydrate chains [14] Molar ratio of ... obtained by N-glycanase digestion of these tryptic peptides We identified the structures of the N-linked chains of ZPA by twodimensional mapping on HPLC referring to the reported structures of the ... detect the shift of the 21 kDa band, 2.5 mU of N-glycanase was added to lg (56 pmol) of ZPA To detect the shift of the 63 kDa band, 0.25 mU of N-glycanase was added to lg (14 pmol) of ZPA Tryptic...

Ngày tải lên: 21/02/2014, 03:20

... top of old ones in an attempt to fix instead of scrap, poor policies While econometric models of proposed supply control policies can be helpful, by necessity they represent a simplification of ... are not completely risk adverse; they prefer to shoulder some level of risk as a tradeoff for the possibility of higher profits.33,34 Supply control programs typically reduce risk for dairy farmers, ... trending higher in all of the examined countries The productivity of cows is driven by a number of factors The largest two influences on productivity are the type and nutrient content of the feed they...

Ngày tải lên: 22/02/2014, 05:20

... determined by Edman degradation of the intact protein, thereby allowing deduction of the sequence of a mature protein consisting of 143 residues (AB201768) The basic Y7 cDNA encoded a mature protein of ... by an abundance of Ser (9– 12%) The members of acidic DE group had a high content of Asp (14%) and Glu (10–14%) N-Terminal amino acid sequence analysis of HMM chitin-binding proteins Eleven of ... mass of about 25 kDa, and were characterized by an abundance of Gly near the amino terminus The N-terminal 78 residues of basic G13, for example, contained 34 Gly residues The four proteins of...

Ngày tải lên: 07/03/2014, 21:20

... Hydrolysis of retinyl palmitate by carboxylesterases (Eur J Biochem 269) 4397 Fig Phylogenetic tree for rat liver carboxylesterases The phylogenetic tree was generated by MACVECTOR 7.0 software ... The new carboxylesterase has molecular weight of 58 kDa as determined by SDS/PAGE and pI of 6.0 (Table 4) Analysis of the new protein by SCANPROSITE software (http://www expasy.ch/tools/scnpsit1.html) ... carboxylesterases were incubated for 10–60 with 0.3–20 lM of retinyl palmitate The reactions were stopped by addition of mL of acetonitrile Ten microliters of 10 lM all-trans-retinal in dimethylsulfoxide...

Ngày tải lên: 08/03/2014, 10:20

... ethereal solvent, tetrahydrofuran (THF), reported in detail elsewhere [14,15] This polymer is composed of a randomly constructed network of tetrahedral hybridized phenylcarbyne units and can be converted ... hundreds of nanometers Most of them are straight and have a smooth surface Fig 3b shows a TEM image of individual curly wire with the average diameter of 400 nm, revealing the typical structure of ... deionized water, ethanol, and acetone, to allow the formation of the polymer film with the thickness of 200 nm after the removal of the THF by evaporation at 60 °C The polymer film on the silicon substrate...

Ngày tải lên: 16/03/2014, 15:06

... crystal nucleus formed by the reaction of Fe2+ with O2 and OH- produced by the hydrolysis of CH3COO- Then these R-FeOOH particles further assembled into rodlike structures by combining together ... porosity of type IV with a distinct hysteresis loop in the range of 0.5-1.0 P/P0.36 The curve of pore size distribution of the porous R-Fe2O3 nanorods is shown in the inset figure The curve exhibits ... results of TG-DTA 17806 J Phys Chem C, Vol 112, No 46, 2008 Wang et al Figure Schematic diagram of the formation mechanism of the porous R-Fe2O3 nanorods Figure (a, b) TEM and HRTEM images of R-FeOOH...

Ngày tải lên: 19/03/2014, 16:48

... How can the benefits of e-CRM for banks be described? 7.2 How can the benefits of e-CRM for bank customers be described? References 54 56 56 57 60 List of figures e-CRM 11 List of Tables 6.3 cross ... can the benefits of e-CRM for banks be described? RQ2: How can the benefits of e-CRM for bank customers be described? 1.4 Delimitation Due to the fact that research in benefits of e-CRM is a ... is often impractical and impossible or too expensive to collect data from all the potential units of analysis included in the research problem Thus, from smaller number of units, a sample, as often...

Ngày tải lên: 22/03/2014, 21:20

... TUBBY-like genes in rice Q Liu performed This first description of the whole rice TUBBY-like gene family will aid in our understanding of the function of TULPs in plants Results ... members of the rice TUBBY-like family The results of expressed sequence tag (EST) and cDNA blast searches showed that 13 of the 14 OsTLPs matched at least one significant EST sequence, and 11 of the ... 2007 FEBS Q Liu TUBBY-like genes in rice Fig Phylogenetic tree of eukaryote TUBBY domains The neighbour-joining method wrapped in PHYLIP [28] was used to reconstruct the phylogenetic tree based...

Ngày tải lên: 23/03/2014, 07:20