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... were designed for quantitative PCR as shown in Table Sequence of PAO651f is the reverse complement sequence of PAO651 probe Sequence of PAO846r is the same sequence of PAO846 probe For the quantitative ... values for the DNA standards versus logarithm of their starting copy number was constructed A typical linear regression line is shown in Figure The averages of R2 value and the slope of the calibration ... photographs were taken of each sample The areas of each picture image that were positive for the PAOMIX and EUBMIX probes were measured with image analysis software (Qwin, Leica) The ratios of PAOMIX-positive...

Ngày tải lên: 05/09/2013, 09:38

... find the Web Service solution in a separate location called SecurityWebServices on the Web site The Chapter 13 solution will contain the sample forms that are created to call methods from the Web...

Ngày tải lên: 24/12/2013, 06:17

... Step Test the MOTD display a Exit the console session Reenter the router to display the message -of- the- day This is done by pressing the Enter key This will display the message entered into the configuration ... combinations of configurations for each router class What is provided are the identifiers for the possible combinations of interfaces in the device This interface chart does not include any other type of ... _ c Save the configuration information from the privileged EXEC command mode Upon completion of the previous steps, logoff by typing exit Turn the router off 2-4 CCNA 2: Routers...

Ngày tải lên: 24/01/2014, 19:20

... message of the day This is done by pressing the Enter key This will display the message entered into the configuration Step Verify the MOTD by looking at the router configuration a Enter the show ... combinations of configurations for each router class What is provided are the identifiers for the possible combinations of interfaces in the device This interface chart does not include any other type of ... exactly how the router is configured, look at the interfaces This will identify the type of router as well as how many interfaces the router has There is no way to effectively list all of the combinations...

Ngày tải lên: 24/01/2014, 19:20

... Design of the study The research is organized in three main parts Part is the introduction which is concerned with presenting the rationale, the scope of the study, the aims of the study, the methods ... of the study, the methods of the study and the design of the study Part is the body of the thesis which consists of three chapters Chapter reviews relevant theories of language teaching and testing, ... from the easiest to the most difficult ones and the test items, therefore, may cover the content of the course book Or in other words, the test has content validity Besides the mean, the mode, the...

Ngày tải lên: 06/02/2014, 14:45

... worse: the vast majority of economists never even bother to ask the question of the proper scale of the economy relative to the environment In standard economic theory the economy can simply grow forever, ... hand over the land, they wanted the government to give them control of the land but not responsibility for it, and they wanted an increase in the fat subsidies they enjoyed for grazing their sheep ... management plan for the forests within the range of the northern spotted owl that would meet the legal requirements of the Endangered Species Act, while also recognizing the needs of communities...

Ngày tải lên: 17/02/2014, 17:20

... Receiving the equivalent of 15 mg /day or more of prednisone for at least one month o Have a history of organ transplant • Have signs or symptoms of active TB, regardless of the size of induration of the ... Needing Evaluation for Treatment (LTBI)” to the TB Control Program of the local health department The referral for evaluation for treatment of LTBI is recommended, not required Part of Form I-693 should ... only for required referrals, therefore the civil surgeon should not complete Part of Form I-693 when making this referral It follows that the health department is not required to complete Part of...

Ngày tải lên: 06/03/2014, 04:20

... distal part is unique for GIRK1 among the Kir3.x isoforms Further truncations of the C-terminus revealed a 49 amino acid stretch (amino acids 362–411) that was the most prominent target for phosphorylation ... [18] To assess the role of the S ⁄ Ts in the regulation of GIRK1 via PKA in a manner that is unbiased by the eventual contributions of the other subunits in a heterooligomer, the corresponding ... previously Plasmids for the production of recombinant protein were constructed by a PCR, where forward and reverse primers encoded the desired parts of the C- and N-termini of GIRK1 These sequences...

Ngày tải lên: 07/03/2014, 00:20

... mixture of the sine curve of analogue and 0/1 values of digital It also refers to the integration of AV and IT Idei envisioned the V AIO as a platform for a network service over which Sony could offer ... however; these properties are not themselves the success factors Rosenzweig calls the tendency to observe the results first and then rationalize the company’s strategies the Halo Effect.14 The real ... nowhere? The media have speculated about why the fortunes of these two companies changed so rapidly, but their evaluations are usually superficial and focus only on short-term performance Often,...

Ngày tải lên: 14/03/2014, 14:02

... deficiencies in the Department’s operations and the need for corrective action The Office of the Deputy Inspector General for Auditing is one of six components within the Office of the Inspector ... that performs oversight of the Department The Office of the Deputy Inspector General for Auditing conducts audits on all facets of DoD operations The work results in recommendations for reducing ... associated with the particular scope of the audit One significant theme for the FY 2013 audit plan was to focus on the current budget challenges faced by the Department and the need to parallel the Secretary...

Ngày tải lên: 15/03/2014, 23:20

... constructed, differing only in the particular combination of side-chain conformers, being each of them optimized by energy minimization in the presence of GDP with the CHARMM27 force field [43] Site-directed ... parameters in the forward reaction The Vm, Km and catalytic efficiency (Vm ⁄ Km) values for nucleotides were determined in the forward and reverse reactions for the wild-type and the five EhPGK ... surrounding the purine nucleotide pocket in PGKs (A) Partial alignment of the C-terminal domain of several PGKs The EhPGK amino acid sequence alignment was performed with the CLUSTALW2 tool and the indicated...

Ngày tải lên: 16/03/2014, 01:20

... the actual motion of the molecules, or because of the motion of the free electrons if they are present Therefore, this form of heat transport depends heavily on the properties of the medium and ... (because of the presence of the T term) The solution of this equation gives the steady state temperature of the plate If we want to calculate the temperature of the plate as a function of time, ... gas; mw , the mass of the wall of the bulb; cpw , the specific heat of the wall; hf , the heat transfer coefficient between the filament and the gas; hg , the heat transfer coefficient between the gas...

Ngày tải lên: 17/03/2014, 13:53

Ngày tải lên: 18/03/2014, 09:42

... Studies of the biology of cancer have informed the biology of aging and vice versa A deeper understanding of the biology of aging has the potential to affect not just cancer, but all of the disorders ... modeled the likelihood they got the condition in the next year Health Status Transition Model The FEM then predicts the health conditions and functional status of the baseline sample for the next ... prevention of disease was the subject of breakthroughs in all three of the medically focused panels These breakthroughs include the prevention of cardiovascular disease, the prevention of a variety of...

Ngày tải lên: 22/03/2014, 13:20

... systematic effort has hitherto been made for the exploration of the entire group Under the direction of the Dominion Government, the waters and shores of the north and east coast of the islands ... by the sinking of the earth’s surface, and the inflowing of the waters of the ocean, forming the most remarkable labyrinth of inlets, sounds, straits, channels and passages on the face of the ... the 5th of May, having secured the services of two Hyda Indians, one a native of Ninstints, the extreme southern village of the Hyda nation, familiar with the shores of the southern portion of...

Ngày tải lên: 23/03/2014, 08:21

... defined. The approach splits the research into two main parts. The first part covers the design of an integrated ADAS  system. The second part covers the design of interface concepts for the ADAS system.  System Concept The first part, the design of an ADAS system started with the investigation of user and stakeholder requirements. It was  ... interface has to be designed The research area therefore consists of two major parts, namely the design of the underlying system, and the design of the user interface. For future reference, the underlying system will be called 'system concept', the user interface will be  ... These concluding requirements are the result of current research, and apply to a 'general public'. The objected user  group of the Car of the Future is part of the general public, so these characteristics can be added to the total user  image. Other findings from literature turn out to correspond to the ones found in the Nexus vision...

Ngày tải lên: 23/03/2014, 10:20

... systematic effort has hitherto been made for the exploration of the entire group Under the direction of the Dominion Government, the waters and shores of the north and east coast of the islands ... by the sinking of the earth’s surface, and the inflowing of the waters of the ocean, forming the most remarkable labyrinth of inlets, sounds, straits, channels and passages on the face of the ... the 5th of May, having secured the services of two Hyda Indians, one a native of Ninstints, the extreme southern village of the Hyda nation, familiar with the shores of the southern portion of...

Ngày tải lên: 23/03/2014, 10:21

... transformed with the bait plasmid pHybLex/NUB1 The resulting transformant was used as a host for the following transformation with the prey plasmid pGAD10 containing the cDNA library As the mRNA of ... that of NUB1 with the exception of the UBA2 insertion, we hypothesized that the immunoprecipitates of NUB1L had the same enzymatic activity as those of NUB1 To prove this, we performed the in ... USA) To make the cDNA of (Ub)2, the cDNA of (Ub)3 was digested with BstXI, the cDNA of a single ubiquitin unit was removed from that of (Ub)3, and the cDNA was ligated Construction of mammalian...

Ngày tải lên: 23/03/2014, 12:20

... v)) The denominators in both terms are for normalization C is the set of all sentences in the collection The value of the parameter d which we call “bias”, is a trade-off between two terms in the ... although the two STs of Figure share most of the subtrees rooted in the ST node, the kernel defined above computes no match The critical aspect of the TK function is that the productions of two evaluated ... where the i-th element vi (T ) is the number of occurrences of the i-th tree fragment in tree T The tree fragments of a tree are all of its sub-trees which include at least one production with the...

Ngày tải lên: 23/03/2014, 17:20

... splicing site at the beginning of the exon We obtained the 3¢ lacking part of this mRNA by 3¢ RACE-PCR As shown in Fig 3, the 3¢ end of this transcript (K3g) was identical to the 3¢ end of the major ... denote the new or earlier described variants for which new data are given in the text The length in nucleotides (nt) of the cloned sequences, without the poly(A) tail, is shown at the left of the ... Alignment of the predicted hKLK3 proteins The signal peptide is highlighted in yellow and the propeptide in blue The amino-acid residues of the catalytic triad are in red, while the binding site for the...

Ngày tải lên: 23/03/2014, 20:22