... oxidase and its possible alternatives, (b) the size and extent of the acidic patch on subunit II, and (c) ways of experimentally influencing the kinetic phases during enzyme turnover MATERIALS AND ... wild-type and mutant enzymes (Tables and 2) This analysis reflects and con- firms the kcat values obtained from turnover experiments Some of the mutants showed extremely slow reduction behaviour, and ... (oxidation of cytochrome c) and/ or 605 nm (reduction of heme a), and biphasic time courses were obtained Three independent measurements were done for each donor concentration, and the average was fitted...
... Negative difference bands at 1525 and 1347 cm)1 refer to the symmetric and anti-symmetric vibrations of the NO2 group in cgATP identied previously [25] For comparison and further band assignment, ... intermediate I and Pi are proportional to the absorption changes at 1255 cm)1 (vas a-b-ATP band), 1338 cm)1 (unidentied protein side chain band) and 1078 cm)1 (inorganic phosphate band), respectively ... of CopB-B with ATP and c-18O4-ATP, respectively The positive band at 1137 cm)1 observed in the 16O compound is downshifted to 1089 cm)1 in the c-18O4-labelled ATP, and this band can therefore...
... Brilliant Blue and 10 ng of protein for Western blotting M, Molecular mass markers; lanes and 6, wild type DHAR; lanes and 7, C9S DHAR; lanes and 8, C23S DHAR; lanes and 9, C26S DHAR; and lanes and 10, ... that contained the fragment as template and the following primers: for C9S, P2 and P3; for C23S, P1 and P4; for C26S, P1 and P5; and for C9S/C26S, P1, P2, P3 and P5 The oligonucleotide primers sequences ... plots between m1 and the concentration of GSH, and double-reciprocal plots between m1/m2 and the concentration of GSH The slope and intercept of the former GSH1 GSH2 plot gave Vmax and Km þ Km while...
... dyes and colors It is also used as a developer in photography and holography; a mordant for dying wool; a chemical reagent for antimony and bismuth; and as an active reducer for gold, silver and ... of the reaction and concentration of the reactants: Equation ν = k cAmcBn Where cA and cB are concentration of the reactants, A, and B, in a reaction with stoichiometry m and n, and k is the reaction ... institute of standards and industrial research, Prof Lee Kum-Tatt, for his precious guidance and collaboration in this work Also my heartfelt gratitude goes to Ms Lee Chooi Lan and Ms Lew Huey...
... 350.82098/L63 Small and Medium Enterprise - Monitoring and Evaluation – Mexico Small and Medium Enterprise - Monitoring and Evaluation – Chile Small and Medium Enterprise - Monitoring and Evaluation ... Colombia Small and Medium Enterprise - Monitoring and Evaluation – Peru Mexico – Small and Medium Enterprise – Monitoring and Evaluation Chile – Small and Medium Enterprise – Monitoring and Evaluation ... Yevgeniya Savchenko and ITESM consultants Jorge Mario Soto, Hugo Fuentes and Victor Aramburu, and by our World Bank colleagues Anne Pillay, Rosa Maria Hernandez-Fernandez and Lucy Bravo Special...
... quantity and quality of wastewater treated in the STP and the regeneration of the Wonderland Park Assuming that the relationship between utility and attributes is linear in the parameters and variables ... economic value of improved water quality in general (e.g., Fraas and Munley, 1984; Fernandez, 1987; Wang, 2002; Ha and Bae, 2001; Day and Mourato, 2002; Colombo et al., 2005; Hanley et al., 2005; ... presents the case study of Chandernagore municipality Section explains the choice experiment method and survey design and administration The results are presented in section and section concludes the...
... day-to-day reference and as a basis for training sessions and individual study and discussion; • Ensure staff adopt a consistent approach to planning and performing an audit; and • Improve communication ... INTERNATIONAL STANDARDS ON AUDITING IN THE AUDITS OF SMALL- AND MEDIUM-SIZED ENTITIES • Performing risk assessment procedures to understand the business and identify inherent and control risks; ... planning and performing risk assessment procedures; understanding the client; responding to risks; evaluating audit evidence; and reporting In addition, the Guide offers some useful practice aids and...
... lettering and, the 10-mer substrate is in lowercase lettering, and the ribozyme recognition elements recognition element RE1 and RE3 base pair with the substrate to form helices P1 and P10, respectively ... substrate and products were denatured at 90 C for and then separated on a 12.5% denaturing polyacrylamide gel The bands were visualized on a Molecular Dynamics Storm 860 Phosphorimager and quantied ... (D) and 300 nM (d) Observed rate constants (kobs) were obtained from these plots and are the average of two independent assays All data points between the two independent assays have a standard...
... forward and M13 reverse, respectively, and two partial DNA fragments were amplified Subsequently, PCR was performed with these two amplified fragments as the templates and primers, M13 forward and ... the kinetic parameters of wild-type and W402F IPU for panose, mixtures consisting of lgÆmL)1 enzyme and from 43 to 216 mM substrate, and 20 lgÆmL)1 enzyme and 64 to 480 mM substrate, respectively, ... TLC The W402F enzyme liberated isopanose from pullulan, and isomaltose and glucose from panose, and the action patterns of the wildtype and the W402F enzymes were almost identical (Fig 2) The...
... 2009) and who they are not (Berger and Heath 2007) Research on brand identity reveals how meaning can be transferred from a reference group who uses a brand to the brand (Muniz and O’Guinn 2001) and ... selfaffirmation and self-disaffirmation conditions The affirmation and disaffirmation manipulations we use follow those employed by Steele and Liu (1983) and Liu and Steele (1986) and those used ... holder and whether “polarized to prevent glare” for the sunglasses) and the brand as presented with the brand name in its logo format For the toothbrush holder the brands were Oral-B and Generic,...
... 2B) Reduced F166Y has typical absorption maxima of CDH at 562, 533 and 428 nm due to a-, b- and c-(Soret) bands, respectively, and the absorption decreased at around 480 nm mainly because of flavin ... the WT enzyme [24] and suggest that both flavin and heme are active in the mutant enzyme Presteady-state and steady-state kinetics of F166Y The presteady-state reduction of flavin and the subsequent ... isosbestic points in the 500–600 nm region, where the a- and b-bands of heme absorb We selected 549.0 and 556.7 nm to monitor cytochrome c and heme in CDH, respectively, because these gave the maximum...
... C1 and C2; the double bond between C4 and C5 is not susceptible to reduction, and thus progesterone and related steroids (e.g 4-androstene3,17-dione), which lack a double bond between C1 and ... binding and catalysis Thus, to ascertain the role of each histidine residue, and to identify any differential contribution to binding and catalysis, we isolated the two mutant enzymes H181A and H184A ... steroids are prednisone, progesterone and 1,4-androstadiene-3,17-dione (B) Sequence of PETN reductase and some PETN reductase related enzymes in the region of His181 and His184 The proteins are PETN...
... swinging door for ligand entry J Mol Biol 207, 459463 Tian, W.D., Sage, J.T & Champion, P.M (1993) Investigations of ligand association and dissociation rates in the ễopenế and ễclosedế states ... The optical absorption and oxygen partial pressure data were acquired in real time on a microcomputer, and the results were stored on disks for further analysis Stopped-ow and rapid-scan spectroscopy ... stability of the bound dioxygen Among those components, hemoglobins V and VII are the major ones in each different group, and make up 15% and 30%, respectively, of the total hemoglobin concentration in...
... (AUH) and rat liver arginase (1rla) H, S, T and stands for a-helix, strand, turn and 310-helix Conserved residues are marked by an asterisk Identical behaviour was previously described, and confirmed ... ACCTATGCG-3¢) and the 3¢ complementary primer 5¢-AT TAATGGCATGCTTTACCCGT-3¢ Using the PCR products of agmatinase with the GfiA and CfiT substitutions in the coding and noncoding strands, respectively, and ... aligned with the sequences of 1CEV and 1RLA and pasted into the structural alignment The alignment was then corrected by hand Since the sequences of the two arginases and agmatinase greately differ...
... ! glutamate ỵ NH3 ẵ8 and ATP ! ADP ỵ Pi Isothermal titration calorimetry (ITC) assay of glutaminase activity and CTP synthesis CTP synthase at concentrations between 0.029 and 1.16 lM was loaded ... yeast [11] and mammalian [12,13] enzymes that all require the presence of UTP and/ or ATP to form tetramers Therefore, the L lactis CTP synthase is an attractive candidate for mechanistic and structurefunction ... containing between 1.7 and 17 lM CTP synthase and integrating the entire heat evolvement over time For the measurement of DH for CTP synthesis the enzyme was incubated with ATP, UTP, GTP and MgCl2 as described...
... These cases were as follows, two each of asthma, pneumonia and cancer lung, one each of post CABG, COPD and lung abscess and 12 with cough and cold All samples from these individuals had tested negative ... like to thank Dr A K Chakraborty and the anonymous reviewers for comments and suggestions on the manuscript, Dr N S Deodhar for comments and suggestion on the work and Drs Anil Ravetkar, former ... single gold standard was available for comparison of the performance of the individual tests, an analysis of results was done using a variety of standards Efficiency of microscopy, culture and PCR...
... ED>JUNE 2001 S DIA/FA S ORG/FS AND L1 S INFOCHEM/FS AND L1 S INPI/FS S REACTION WITH/IT S JACSAT/ISN S 0002-7863/ISN CT, IT S 1-3/IS S J AM CHEM SOC/JT S L1 AND EN/LA S L1 AND ENGLISH/LA S MARPAT/OS ... FCRD FCRDREF Compact display of all hit reactions CRD and SO and Publication Date for Reference First Hit Reaction in Compact Format FCRD and SO and publication date for reference (FCRDREF is the ... analyzes the CODEN and ISSN and appends /ISN to the terms created by SELECT Selects or analyzes the ISSN and appends /ISN to the terms Selects or analyzes the IC, ICA, ICI and appends /IPC to...