post translational regulation of nitrogenase in photosynthetic bacteria
Báo cáo y học: "Post-translational aging of proteins in osteoarthritic cartilage and synovial fluid as measured by isomerized aspartate" pot
Ngày tải lên :
09/08/2014, 14:20
... further analysis Determination of protein content Total protein was determined using the commercially available BCA™ Protein Assay Kit in accordance with the instructions of the manufacturer (Pierce, ... http://arthritis-research.com/content/11/2/R55 Figure increasing disease severity This is exactly the result we find The authors of that study further found that the hyaluronanbinding (G1) domain of aggrecan is retained early in the disease ... previously stained with Indian ink, were cut into 3-mm-thick pieces and embedded in Tissue Tek OCT Compound (Sakura Finetek USA, Inc., Torrance, CA, USA) using Peel-A-Way Disposable Embedding Molds...
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Post translational regulation of the human TRIP br1 cell cycle protein
Ngày tải lên :
14/09/2015, 13:56
... protein level (post- translational regulation) Post- translational regulation of proteins can be envisaged to include three main aspects: 1) alterations in sub-cellular localization; 2) post- translational ... shown in the schematic above, namely, cA, Cyclin A binding site; DB, DNA binding domain; DIM, dimerization domain; TA, transactivation domain; PB, pocket protein (pRB family) binding domain Number ... showing the functional domains and phosphorylation events impinging on MDM2 [60] The amino acid residues are numbered Functional domains of the MDM2 protein including the p53 binding domain,...
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Tài liệu Báo cáo khoa học: Complex transcriptional and translational regulation of iPLA2c resulting in multiple gene products containing dual competing sites for mitochondrial or peroxisomal localization docx
Ngày tải lên :
19/02/2014, 16:20
... and 3) Translational regulation of iPLA2c in myocardium Owing to the obvious complexity of the regulation of iPLA2c resulting from the combined presence of transcriptional and translational regulation, ... start of exon resulting in loss of the AUG initiating translation of the 88 kDa isoform We specifically point out that differential utilization of exon vs exon in splice variants introduces differing ... presence of multiple iPLA2c protein products in mitochondria resulting from usage of different translation initiation codons in rat myocardium Alternative splicing of iPLA2c in mammalian tissues In...
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Tài liệu Báo cáo khoa học: Post-translational cleavage of recombinantly expressed nitrilase from Rhodococcus rhodochrous J1 yields a stable, active helical form ppt
Ngày tải lên :
19/02/2014, 00:20
... the interacting residues Examination of the complexes formed prior to post- translational cleavage suggests that steric hindrance resulting from the C-terminal 39 amino acids causes failure of ... Caspar [33] in 16 turns and enables indexing of the power spectrum (Fig 2E, insert) as shown in Fig 3A The clear diffraction spot with a spacing of 7.7 nm is interpreted as a Bessel function of order ... approximate regions of interacting surfaces A, C and D are indicated on the top line Charged residues which are possibly involved in interactions at the D surface are indicated in bold and the external...
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Báo cáo Y học: Regulation of RAS in human platelets Evidence that activation of RAS is not sufficient to lead to ERK1-2 phosphorylation pot
Ngày tải lên :
08/03/2014, 16:20
... Activation of RAS was evaluated in platelets through the ability of the activated RAS–GTP to bind a GST fusion protein containing the RAS-binding domain of RAF Different platelet agonists including ... c-chain [11,12] As expected, the SRC kinase family inhibitor PP1 inhibited convulxin-induced ERK and RAS activation (Fig 6A,B) Interestingly, although activation of ERK in response to thrombin ... Thombin, convulxin and TPO induce activation of RAS RAS activation was measured through the ability of its activated form (RAS–GTP) to bind to a GST fusion protein consisting of the RAS-binding...
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Báo cáo khoa học: Factor-inhibiting hypoxia-inducible factor (FIH) catalyses the post-translational hydroxylation of histidinyl residues within ankyrin repeat domains pot
Ngày tải lên :
28/03/2014, 23:20
... Proteomics-based identification of novel factor inhibiting hypoxia-inducible factor (FIH) substrates indicates widespread asparaginyl hydroxylation of ankyrin repeat domain-containing proteins Mol Cell Proteomics ... uptake repressor, PerR in Bacillus subtilis, which is inactivated by oxidation of the imidazole rings of two Fe(II)-binding histidinyl residues resulting in suppression of peroxidedefence genes ... RT, Ley SC et al (2006) Posttranslational hydroxylation of ankyrin repeats in IkappaB proteins by the hypoxia-inducible factor (HIF) asparaginyl hydroxylase, factor inhibiting HIF (FIH) Proc Natl...
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Báo cáo sinh học: "Regulation of metabolism in Caenorhabditis elegans longevity" ppsx
Ngày tải lên :
06/08/2014, 19:21
... Possible link between accumulation of amino-acid pools, mitochondrial function, and the insulin and TOR pathways in C. elegans longevity Black lines indicate the activating or repressive function of ... in amino acid levels, especially branched-chain amino acids Falk et al [9] showed that mutations affecting complexes I, II and III of the electron transport chain (ETC), including some that increase ... Mutations in ife-2 would also result in increased amino-acid pools Branched-chain amino acids and longevity Fuchs et al [7] may, in fact, hold a clue to one of the mysteries of the aging field:...
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Báo cáo y học: "Expression and regulation of CCL18 in synovial fluid neutrophils of patients with rheumatoid arthritis" potx
Ngày tải lên :
09/08/2014, 10:21
... expression in SF PMN occurs as a result of the recruitment of PMN into the inflammatory milieu of the joint Differential expression of chemokine receptors in SF PMN To estimate changes in the responsiveness ... protein after 24 or 48 hours of incubation (data not shown), indicating that TNF-α induced CCL18 mRNA expression in the absence of protein synthesis Interestingly, as shown in Figure 4a, neither blood ... transcriptase (Qiagen) in a final volume of 25 μl at 37°C for 60 minutes and at 93°C for minutes PCR was performed in a 25 μl reaction mixture containing μl of cDNA sample, 0.625 U of Taq polymerase...
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Báo cáo y học: "Expression and regulation of neurotrophins in the nondegenerate and degenerate human intervertebral disc" pdf
Ngày tải lên :
09/08/2014, 11:20
... studies investigating innervation in the degenerate IVD have also demonstrated expression of Trk-A, the highaffinity receptor for nerve growth factor (NGF), on ingrowing nerves – indicating that ... neurotrophin in IVD degeneration driving pain-related processes as well as neuronal survival and innervation Few studies have examined the function of neurotrophin expression in cells of non-neural ... of neurotrophin expression in the IVD, yet TNFα may have an influential role in regulation of nociception through substance P Competing interests The authors declare that they have no competing...
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Cyclic nucleotides and mitogen-activated protein kinases: regulation of simvastatin in platelet activation pptx
Ngày tải lên :
10/08/2014, 05:21
... Triflavin inhibits platelet aggregation through direct interference with fibrinogen binding to the αIIbβ3 integrin [10] There is now a multitude of evidence suggesting that the binding of fibrinogen ... prostaglandin E1 (PGE1) (0.5 μM) and heparin (6.4 IU/ml) The washed platelets were finally suspended in Tyrode's solution containing bovine serum albumin (BSA) (3.5 mg/ml) The final concentration of ... Simvastatin (30 and 50 μM) did not significantly affect FITC-triflavin binding to the αIIbβ3 integrin in platelet suspensions (c, 4.8 ± 0.1; d, 4.9 ± 0.1) (Fig 1C), indicating that the inhibitory...
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Báo cáo y học: "Stanniocalcin-1 promotes tumor angiogenesis through up-regulation of VEGF in gastric cancer cells" ppt
Ngày tải lên :
10/08/2014, 05:21
... staining of STC-1 in tumor tissues of nude mice STC-1 was detected on the membrane of tumor cells (D) Immunohistochemical staining of PCNA in tumor tissues of nude mice PCNA was detected in the ... Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, PR China 2First Department of Respiratory Medicine, Nanjing Chest Hospital, 215 Guangzhou Road, Nanjing, PR China Authors’ ... ligand-receptor interaction, was obtained from Epitomics Company Cell Apoptosis kit was obtained from MBL International Corporation (Watertown, MA) The recombinant human stanniocalcin-1 protein was obtained...
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Báo cáo y học: " Post-translational modifications of histones H3 and H4 associated with the histone methyltransferases Suv39h1 and G9a" pdf
Ngày tải lên :
14/08/2014, 08:20
... likely influence subsequent modifications, regulated by both cis and trans mechanisms Characterizing such modifications could provide insight into the roles of chromatin-binding proteins In this ... (KDa) (b) Figure chromatin-binding proteins Suv39h1 and G9a Post- translational modifications of histones H3 and H4 associated with the Post- translational modifications of histones H3 and H4 associated ... weight markers (Invitrogen); lane 2, Suv39h1 complex from chromatin fractions (c) Amino-terminal lysine methylation of histones H3 and H4 associated with Suv39h1 or G9a proteins (d) Posttranslational...
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Báo cáo y học: "Global nucleosome distribution and the regulation of transcription in yeas" doc
Ngày tải lên :
14/08/2014, 14:21
... should be taken into account when interpreting genome-wide experiments involving post- translationally modified nucleosomes When examining the distribution of modified histones in the genome, one ... differential segregation of intergenic regions of the genome into the aqueous phase Nagy et al [13] proposed that this may be a result of different efficiency of chromatin cross-linking along the genome ... change in nucleosome occupancy in a typical yeast gene in different transcriptional states (a) When there is no transcription, repressor proteins bind to their DNA-binding sites and maintain a...
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Báo cáo y học: "Translational regulation of gene expression" docx
Ngày tải lên :
14/08/2014, 14:21
... laboratory of Robert Singer (Albert Einstein College of Medicine, New York, USA), described how the trans-acting factor zipcode-binding protein (ZBP1), which binds to the 3Ј UTR of -actin mRNA, ... functions of these RNA-binding translational activators and repressors, as well as the intricacies of their RNA targets One of the best characterized models of translational control involving cis-acting ... phosphorylating ZBP1 are involved in this regulation Serine phosphorylation of ZBP1 by extracellular-regulated kinases (ERKs) induces repression of translation In contrast, tyrosine phosphorylation of...
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REGULATION OF CHEMICALS IN EUROPE – REACH
Ngày tải lên :
16/04/2015, 09:05
... competitiveness of the EU chemical industry Prevention of fragmentation of the internal market Increased transparency Integration with international efforts Promotion of non-animal testing 10 ... human intake No implicit assumption about a ”safe” intake 37 INTERPRETATION OF MARGIN OF EXPOSURE • Inter-species and intra-species differences • 100 would be sufficient • Nature of the carcinogenic ... curve for comparison with human intake • Estimation of human exposure • Calculation of an MOE 36 MARGIN OF EXPOSURE (MOE) Definition: The ratio between the defined point on the dose-response curve...
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Understanding the regulation of cytokinesis in fission yeast
Ngày tải lên :
11/09/2015, 09:06
... chain Myo2p; regulatory light chain of myosin Rlc1p; essential light chain of myosin Cdc4p; IQGAP-related protein Rng2p; UCSdomain containing protein Rng3p; alpha-actinin Ain1p; and paxillin ... regulator of cytokinesis Cells overexpressing Nuc2p phenocopied sin mutants in that the actomyosin rings were not maintained upon completion of mitosis Examination of SIN proteins in the temperature-sensitive ... actomyosin-based contractile ring of fission yeast consists of a number of proteins: F-actin; profilin Cdc3p; tropomyosin Cdc8p; formin Cdc12p; cofilin Adf1p; FER/CIP homology protein Cdc15p; myosin...
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Role and regulation of juxtanodin in actin cytoskeleton of oligodendrocyte
Ngày tải lên :
11/09/2015, 10:18
... polymerization of actin filaments could be inhibited by some actin-binding proteins (Paavilainen et al., 2004) In cells, a large pool of actin monomers is maintained by actin monomer binding proteins Actin ... expressed: ADF/cofilin, profilin, twinfilin, Srv2/cyclase-associated protein (CAP), WASP/WAVE and verprolin/WIP proteins In cells, profilin, Srv2/CAP, ADF/cofilin and twinfilin are abundant in molar ... presence of two F-actin binding domains (Drenckhahn et al., 1988) However, α-actinin forms the more loosely ordered actin structure of stress fibers α-actinin has a single actin-binding domain in its...
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Regulation of cytokinesis in the fission yeast schizosaccharomyces pombe
Ngày tải lên :
14/09/2015, 14:18
... ring producing two daughter cells of equal size The actomyosin ring consists of about 50 different proteins including actin, actin regulatory proteins, type II myosin heavy and light chains The ... 2004) In SIN mutants, actomyosin rings are formed but ring constriction fails The actomyosin ring disassembles and continuous rounds of nuclear division occur in the absence of cytokinesis resulting ... number of proteins, which are essential for the formation and maintenance of the actomyosin ring They include the myosin 16 heavy chain Myo2p; essential myosin light chain Cdc4p; myosin assembly...
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