Tài liệu Báo cáo khoa học: Expression and function of Noxo1c, an alternative splicing form of the NADPH oxidase organizer 1 doc
... extent of membrane localization of Noxo1b and Noxo1c. The intensities of immunoreactive bands for HA-Noxo1b and HA-Noxo1c in (B) were quantified using a LAS-1000plus (Fuji film) image analyzer and ... Functional Analyses from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and CREST and BIRD projects of JST (Japan Science and Technology Agency). References 1 Segal ... the expression and function of the four splice forms of human Noxo1. The Noxo1c mRNA is also expressed in several Nox1-expressing and Noxa1- expressing human cancer cell lines [the androgen- independent...
Ngày tải lên: 19/02/2014, 06:20
Tài liệu Báo cáo khoa học: A zymogen form of masquerade-like serine proteinase homologue is cleaved during pro-phenoloxidase activation by Ca2+ in coleopteran and Tenebrio molitor larvae docx
... 79-kDa pro-PO and 45-kDa Tm-mas. (A) The 79-kDa and 76-kDa bars indicate Tenebrio pro-PO and PO, respectively. (B) The 55-kDa and 45-kDa bars indicate the zymogen form and cleaved protein of 45-kDa ... in the presence of b-1,3-glucan and Ca 2+ (lane 11 in Fig. 7B). The activation ratio of the 55-kDa zymogen form in the presence of b-1,3-glucan and Ca 2+ wasfasterthaninthe presence of Ca 2+ only ... preincubated with p-NPGB and p-APMSF, andthencalciumandb-1,3-glucanwereaddedtoHW-55S solution, PO activity and proteolysis of the 55-kDa Tm-mas were not observed (Fig. 7C, column 3 and Fig. 7D, lane...
Ngày tải lên: 21/02/2014, 03:20
Báo cáo khoa học: Crystal structures of the apo form of b-fructofuranosidase from Bifidobacterium longum and its complex with fructose pot
... the b-propeller and b-sandwich domains involve contacts of IV and V blades and b-strands (1–6) from the internal b-sheet of the b-sand- wich domain. The last four amino acids of the native sequence ... b-sandwich architec- ture and is composed of two six-stranded antiparallel b-sheets. In both b-sheets the sixth strand is located between strands 1 and 2. Although the antiparallel pat- tern of ... the shape and charge of the active site pocket (Fig. 3A), determined by the conformation, length and sequence of the inter- blade loops (ibL I–V ) and the loops L 2–3 between b-strands 2 and 3 in...
Ngày tải lên: 06/03/2014, 00:21
Báo cáo khoa học: DNA polymerase e associates with the elongating form of RNA polymerase II and nascent transcripts pot
... Pospiech 1 and Juhani E. Syva ă oja 2 1 Biocenter Oulu and Department of Biochemistry, University of Oulu, Finland 2 Department of Biology, University of Joensuu, Finland 3 Biocenter Oulu and Department ... and Department of Pathology, University of Oulu, Finland 4 National University of Ireland, Department of Biochemistry, Cell Cycle Control Laboratory, Galway, Ireland 5 Department of Biochemistry, ... hyperphosphorylated elongating isoform RNA pol IIO RNA pol II exists in a dynamic equilibrium between the hypophosphorylated initiation isoform IIA and the hyperphosphorylated elongating isoform IIO that exhibits...
Ngày tải lên: 07/03/2014, 11:20
Báo cáo khoa học: Purification and structural study of the b form of human cAMP-dependent protein kinase inhibitor pdf
... Zheng 1 and Chaoneng Ji 2 1 Center of Analysis and Measurement and 2 State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, PR China The b form of human ... kinds of proteins in the PKI family. The combined results of FTIR and CD showed that human PKIb contained large amounts of random coil and turn structures, with a small amount of a-helix and b ... contained only small amounts of a-helix and b-structures, but large amounts of random coil and turn structures, which may explain its high thermosta- bility. The details of its conformational changes...
Ngày tải lên: 07/03/2014, 15:20
Báo cáo khoa học: The propeptide in the precursor form of carboxypeptidase Y ensures cooperative unfolding and the carbohydrate moiety exerts a protective effect against heat and pressure pot
... its unglycosylated form, though the DV and DG P values were almost the same. The P m value of proCPY was higher than that of its unglycosylated form. This is due to the higher DV value of the unglycosylated form, ... more pronounced conformational change and/ or a more pro- nounced hydration upon unfolding of the unglycosylated form. At high pressure, in the glycosylated forms the carbohy- drate moiety of CPY and proCPY ... Pressure-induced changes of the centre of the spectral mass of (A) proCPY (d)andDgly proCPY (s)at25°C(B)CPY(d )and Dgly CPY (s)at25°C, and (C) CPY at 60 °C(d). Insert shows an enlargement of the ordinate....
Ngày tải lên: 07/03/2014, 21:20
Báo cáo khoa học: Isothermal unfolding studies on the apo and holo forms of Plasmodium falciparum acyl carrier protein Role of the 4¢-phosphopantetheine group in the stability of the holo form of Plasmodium falciparum acyl carrier protein docx
... pathways of the two forms of Plasmodium falciparum acyl carrier protein, the apo and holo forms, were determined by guanidine hydrochloride-induced denaturation. Both the apo form and the holo form displayed ... structure, on the basis of their percentage contri- butions (65% and 35%, respectively) to the overall structure of the protein. The structures of the major and minor conformations of holo-ACP bear ... standard globular proteins, the apparent molecular mass of holo-ACP dimer and the mixture of holo-ACP monomer and apo-ACP were found to be 33 kDa and 25 kDa, respectively. Purified holo-ACP and...
Ngày tải lên: 16/03/2014, 10:20
Báo cáo khoa học: Co-incorporation of Ab40 and Ab42 to form mixed pre-fibrillar aggregates ppt
... Cancer Institute and Department of Medical Biophysics, and 2 Department of Chemical Engineering and Applied Chemistry, Institute of Biomaterials and Biomedical Engineering, Department of Biochemistry, ... sample preparation. Homogeneous and heterogeneous mixtures of Ab40 and Ab42 formed these spherical prefi- brillar aggregates with a uniform distribution of morphology and size. CD spectroscopy indicates ... mixed molecular species, and the relative ease of formation of mixed vs. homogenous species. We believe that the approach of studying fibrillogenesis in the context of Ab40 and Ab42 mixtures is advantageous. Studies...
Ngày tải lên: 17/03/2014, 09:20
Báo cáo khoa học: Engineering of a monomeric and low-glycosylated form of human butyrylcholinesterase pdf
... for oligomeric forms, even if they form a noncovalent dimer by association of a four-helix bundle ( helices 383±372 and 526±543; human Table 2. I n¯uence of the number and position of N-glycosylation ... 6 months. Each L of culture medium contained 3±5 mg of 4sugOff 17/455/481/486 BChE D . Puriđcation of 4sugOff 17/455/481/486 BChE D Units of activity are expressed as lmoles of substrate hydrolyzed ... stability, and solubility of ChE as well as the clearance of their plasmatic forms [22,33±35]. Heavy glycosylation of BChE contributes to its long residence time in blood circulation and protects...
Ngày tải lên: 17/03/2014, 11:20
Báo cáo Y học: Ubiquitination of soluble and membrane-bound tyrosine hydroxylase and degradation of the soluble form ppt
... membrane-bound tyrosine hydroxylase and degradation of the soluble form Anne P. Døskeland and Torgeir Flatmark Department of Biochemistry and Molecular Biology, University of Bergen, Norway Tyrosine hydroxylase ... (1997) Molecular characterization of disease related mutant forms of human phenylalanine hydroxylase and tyrosine hydroxylase. In Chemistry and Biology of Pteridines and Folates (Pfleiderer,W.& Rokos, ... system 35 S-labelled polypeptides of the expected molecular mass of native enzyme as well as both higher and lower molecular mass forms were observed. The amount of high-molecular-mass forms increased by time and was...
Ngày tải lên: 17/03/2014, 17:20
How to teach countable and uncountable nouns to the 1st year non- English majors of Hai Phong Private University
... salt. - Measures: A kilo of sugar; A meter of cloth; A length of cloth; A little of oil ; An ounce of gold; A pint of milk; A pound of coffee ; - A game of: A game of football, table tennis, ... A bit of advice; A fit of anger; A branch of knowledge A piece of research ; A period of calm; A spot of trouble; - Types and specifics: A kind of biscuit; A species of insect; A type of drug; ... Some nouns non- plurals with „s‟ are uncountable nouns. Uncountable noun do not have regular form and take a singular verb. The singular category includes uncountable nouns and proper nouns. ...
Ngày tải lên: 18/03/2014, 17:21
Báo cáo khoa học: Properties and significance of apoFNR as a second form of air-inactivated [4Fe-4S]ÆFNR of Escherichia coli pot
... The redox state of the cysteinyl residues of apoFNR should be relevant for function and properties of the protein. Therefore the redox state of the cysteinyl residues of the various forms of apoFNR – ... conversion, suggesting that apoFNR is a product of [2Fe-2S]ặFNR decomposition and a final form of air-inacti- vated FNR in vitro. Formation of apoFNR and the redox state of the cysteinyl residues ... five- fold alkylated apoFNR, and a minor signal of three- fold alkylated FNR (Fig. 1B). Therefore, aerobic and anaerobic apoFNR are mixtures of FNR with one, three and five, and five and three cysteine...
Ngày tải lên: 23/03/2014, 15:20
A Study on the Effects of Argentine Tango as a Form of Partnered Dance for those with Parkinson Disease and the Healthy Elderly pptx
... leading cause of injury deaths in older adults (Murphy 2000), and they can lead to fear of falling, reduced quality of life, withdrawal from activities, and injury. Changes in joint ranges of motion, ... task: Interference effects of functional tasks on walking in Parkinson’s disease and the roles of cognition, depression, fatigue, and balance. Archives of Physical Medicine and Rehabilitation, 85: ... Argentine tango. Argentine tango is a form of artistic expression. Soulful, and full of meaning, tango music creates an atmosphere of contemplation, longing and intellectual stimulation. Since a dancer’s...
Ngày tải lên: 28/03/2014, 20:20
Báo cáo khoa học: The soluble form of the membrane-bound transferrin homologue, melanotransferrin, inefficiently donates iron to cells via nonspecific internalization and degradation of the protein pdf
... from sMTf and Tf as a function of ligand concentration The uptake of 59 Fe from Tf and sMTf was also investigated as a function of ligand concentration (0.0010.1 mgặmL )1 ) (Fig. 3A and B). Internalized, ... the Natural Sciences and Engineering Research Council of Canada (NSERC) and by fellowship and grant support from the National Health and Medical Research Council of Australia and an Australian Research ... 11 and 17%. In contrast, the internalization of 125 I-Tf (0.05 mgặmL )1 )was much greater, ranging between 47 and 69% (data not shown). Uptake of 125 I-sMTf and 125 I-Tf as a function of ligand concentration To...
Ngày tải lên: 31/03/2014, 09:20
Báo cáo sinh học: " Possible active origin of replication in the double stranded extended form of the left terminus of LuIII and its implication on the replication model of the parvovirus" pptx
... 5); the larger band migrates along side the band observed for pGLu883∆Xba likely representing the left end hairpin of LuIII in double stranded form. The smaller of the two bands, of greater intensity, ... end of the viral genome primes the syn- thesis of a complementary strand, leading the formation of a double stranded replicative form known as the cRF. In vitro studies have shown that the cRF of ... functionality of the left hairpin of LuIII as an origin of replication in the extended double stranded form a replication model of LuIII can be predicted result- ing in equivalent amounts of plus and minus...
Ngày tải lên: 19/06/2014, 08:20