polysialic acid in eukaryotic cells

... number of cells was analyzed (Fig 2B) In order to investigate whether CEdG has the potential to accumulate in genomic DNA of nongrowing cells, HSC-T6 cells were kept in minimal medium containing 1.5% ... nucleobases were detected in human urine [13], indicating the formation of DNA AGEs in the healthy human organism A significantly increased number of CEdGpositive cells were immunostained in glomeruli of ... stellate cells (HSC-T6) were incubated in growth media (MEM; Biochrom, Berlin, Germany) with Earle’s salts containing 20% fetal bovine serum (Biochrom) and 1% penicillin ⁄ streptomycin solution...

Ngày tải lên: 30/03/2014, 04:20

... Pharmigen, San Diego, CA, USA) binding and PI staining were performed according to [44] Following incubation with 1.0 lgÆmL)1 annexin V and 4.0 lgÆmL)1 PI for 20 min, cells were analyzed by flow cytometry ... results that TAG synthesis was initially hindered following stearate administration and was completely interrupted at longer incubation periods Interestingly, the interruption of TAG synthesis ... uptake into liver cells is the long-chain acyl-CoA synthetase (ACS), which activates fatty acids by linking them to coenzyme A As shown in Fig 6, triacsin C (TrC), a specific competitive inhibitor...

Ngày tải lên: 14/02/2014, 22:20

... inactive [49] In our experiments NDGA was effective in inhibiting the activity of purified recombinant MTE-I in an in vitro assay performed in a Hepes-based buffer containing only KCl Information ... known that an acyl-CoA binding protein (ACBP) known also as DBI (diazepam binding inhibitor) is expressed in high concentrations in specialized cells such as steroid producing cells of the adrenal ... performed in triplicate activity in vitro Figure shows that a 5-min preincubation of recombinant protein with NDGA inhibited thioesterase activity Interestingly, NDGA produced only a 20% inhibition...

Ngày tải lên: 08/03/2014, 09:20

... MA-10 cells were labeled for h at 37 °C with [1-14C] AA (1 lCiÆmL)1 per · 106 cells) in serum-free media containing 0.5% fatty acid- free bovine serum albumin Then, cells were incubated in either ... fatty acids present in an aqueous environment by effectively decreasing the insoluble fraction [2] Albumin, in particular, binds specifically to fatty acids [33] In our experiments, we added albumin ... cultures in a concentration of lCiÆmL)1 per well (2 · 106 cells) in serum-free Waymouth MB752 ⁄ containing 0.5% fatty acid- free bovine serum albumin [17] After h of incubation at 37 °C in a humidified...

Ngày tải lên: 16/03/2014, 12:20

... melanoma cell line B16F10, BA improved vincristine-induced cytotoxic effects in vitro, in addition to reducing the number of metastases in vivo [7] Sublines of the colon cancer cell line SNUC5 that ... 23 μM in U251MG cells and 24 μM in U343MG cells under normoxic conditions after an incubation time of 24 h (Figure 1) In addition, longer incubation with BA led to increased cytotoxicity in both ... significantly increased cytotoxicity when 5-fluorouracil, irinotecan or oxaliplatin were combined with BA treatment [23] In addition, BA augmented doxorubicin- or cisplatin-induced apoptosis in several...

Ngày tải lên: 09/08/2014, 09:21

... Modifying Factors in Asian Indian and Chinese Males Nutrition In press • Manav, HM Shen and CN Ong Involvement of c-Jun N-terminal kinase activation in Docosahexanoic acid- induced apoptosis in colon ... differ in their C-terminal ligand binding domains, and each appears to bind and respond to a specific subset of agents including hypolipidemic drugs, long chain fatty acids, prostaglandins, and ... neuro2A cells from serum deprivation induced apoptosis (Kim et al., 2000) and prevents oxidative stress induced apoptosis in retinal cells (Rotstein et al., 2003) In leukemic cells too, DHA inhibits...

Ngày tải lên: 26/09/2015, 09:57

Ngày tải lên: 04/10/2012, 10:02

... Medicine 57 Reagents F12 culture medium (Hangzhou Jino Biology Co., Ltd China), fetal bovine serum (FBS), ampicillin, penicillin and streptomycin (Shanghai Bio-engineering Co., Ltd China), brain ... O2, 85% N2, 10% CO2) with continuous shaking E coli were maintained in the LB liquid medium (containing 100 µg/ml ampicillin) at 37°C for 12 h with continuous shaking Preparation of Hp and E.coli ... Ltd USA) were used in the present study Cells and Hp Culture AGS cells were grown in the F12 culture medium containing 10% FBS, 100 U/ml penicillin and 100 µg/ml streptomycin in a humidified atmosphere...

Ngày tải lên: 25/10/2012, 11:18

... almost no increase in the phosphorylated JNKs protein level in the cell line Twenty-four-hour exposure to hypoxia resulted in a Int J Med Sci 2007, 42 marked increase (1.8-fold of control) in the ... Morpho/AS was examined using a fluorescent microscope Fluorescence was mainly observed in cytosol areas in transfected cells FITC-labeled Bag-1 Morpho/AS was transfected into almost all JAR cells by EPEI-mediated ... and methods Cell line and cell cultures The human choricarcinoma cell line JAR was obtained from American Type Culture Collection (Manassas, VA, USA) Cells were maintained in RPMI 1640 medium...

Ngày tải lên: 31/10/2012, 15:12

... after its initial effect on protein kinases phosphorylation The most strikingly and novel finding obtained hereby was the strong inhibition of the GPCR signaling pathway induced mainly by increased ... proteins Oncogene 1996;12:1-9 18 Hoek JB, Pastorino JG Cellular signaling mechanisms in alcohol-induced liver damage Semin Liver Dis 2004;24:257-72 19 Aroor AR, Shukla SD MAP kinase signaling in ... of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 1976;72:248-54 24 Pelech S, Sutter C, Zhang H Kinetworks protein kinase multiblot analysis Methods...

Ngày tải lên: 31/10/2012, 15:37

... G Elevated c-Jun expression in acute myeloid leukemias inhibits C/EBPalpha DNA binding via leucine zipper domain interaction Oncogene 2003;22(30):4760-4 Staber PB, Linkesch W, Zauner D, Beham-Schmid ... exon 10 and adjacent intronic sequences we could for the first time identify a new single nucleotide polymorphism (SNP) in the intron sequence flanking the 3’ end of exon 10 in out of 35 (20%) ... approximately 101 bp and 444 bp in size in samples either containing the heterozygous C/T or homozygous T/T SNP while these fragments could not be detected in samples lacking the SNP and therefore...

Ngày tải lên: 31/10/2012, 16:49

... involved in claudin-1 expression and localization 99 Indeed, a study shows that increasing β-catenin increases the expression of claudin-1 in colorectal cancer cells, and that claudin-1 expression ... using bryostatin instead increased the expression of claudin-1 in the tight junction complex, and increased tight junction barrier integrity (9) In melanoma, we have shown that claudin-1 expression ... melanoma cells (4) To determine if the nuclear localization of claudin-1 was related to the increased invasive capacity of melanoma cells, we created a claudin-1 expression vector expressing claudin-1...

Ngày tải lên: 03/11/2012, 11:17

... (GO:0007166) The basigin protein was shown to be present in vascular endothelium in both in the non-neoplastic region of the brain and in tumor cells (http://amigo.geneontology.org/cgi-bin/amig o/gp-details.cgi?gp=UniProtKB:P35613) ... cannot determine the global players involved in such complex phenotypes Integration of the gene expression profiling with specific modulation of gene expression in relevant signalling pathway ... Software and are described in the following part according the definitions of the Illumina’s (ILL) data as well as of the Illumina’s annotations (table 2) http://www.medsci.org Int J Med Sci 2009, 22...

Ngày tải lên: 03/11/2012, 11:44

... on the length of linkers In the linker containing seven amino acids, a Pro-Pro sequence that is not contained in other linkers is incorporated This less flexible structure may inhibit fluorophores ... expressed GAPDH–citrine (chimeric protein with N-terminal GAPDH and C-terminal citrine) and citrine–GAPDH (chimeric protein with N-terminal citrine and C-terminal GAPDH) were localized in the cytoplasm ... obtained when cell extracts containing GAPDH–cerulean (chimeric protein with N-terminal GAPDH and C-terminal cerulean) or cerulean–GAPDH (chimeric protein with N-terminal cerulean and C-terminal...

Ngày tải lên: 16/02/2014, 09:20

... as inactive proenzymes and latency is maintained by an interaction between a cystein residue in the prodomain and Zn2+ in the active site of the catalytic domain Two major types of endogenous inhibitors ... determine the viability of the cells, trypsinized cells were suspended in serum-containing medium In order to remove serum, cells were washed three times with serum-free medium prior to seeding ... inhibitors in several cell lines [10] The protein itself has no known enzymatic activity, but binds to distinct intracellular target proteins and regulates specific functions involved in tumour...

Ngày tải lên: 18/02/2014, 11:20

... highly specific peptidebinding domain found in poly(A)-binding protein and a HECT ubiquitin ligase EMBO J 23, 272–281 Craig A (1998) Interaction of polyadenylate-binding protein with the elF4G homologue ... to interact with eIF4G at a stoichiometry of more than : It should be noted here that, in addition to the C-terminal domain, PABP1 has another eIF4G-binding domain within its N-terminal RNA-binding ... growth medium containing 20% fetal bovine serum was added Following a total of 12 h of incubation, the medium was replaced with fresh DMEM containing 10% fetal bovine serum Cells were usually...

Ngày tải lên: 18/02/2014, 12:20

... to in ammatory sites [14,15] and adhesion of polymorphonuclear cells and monocytes to CNS cells [16,17] Schwann cells (SCs) are glia cells found in the PNS In addition to their roles in myelination, ... al LPS increases ICAM-1 expression in Schwann cells of sciatic nerves A B C D Fig LPS induced the expression of ICAM-1 protein in cultured SCs (A) LPS induced ICAM-1 protein expression in a concentration-dependent ... responses in the PNS [31] These cells may function as antigen-presenting cells and activate T cells in vitro in an antigen-specific and MHC-restricted manner [32], especially in the presence of cytokines...

Ngày tải lên: 18/02/2014, 18:20

... chemical inhibitor SB-431542 to block activin ⁄ TGF-b signaling in murine stem cell cultures Both experiments resulted in a strong growth inhibition of the stem cells but, fascinatingly, did not interfere ... neuroepithelial cells into astrocytes, by binding and hyper activating the promoter of glial fibrillary acidic protein (GFAP) In parallel, BMP signals lead to enhanced expression of ID proteins, which in turn ... protein kinase signaling pathway and induction of neurite outgrowth in PC12 cells by bone morphogenetic protein-2 J Biol Chem 274, 26503–26510 Levine AJ & Brivanlou AH (2006) GDF3, a BMP inhibitor,...

Ngày tải lên: 19/02/2014, 00:20

... cultured in Dulbecco’s modified Eagle’s medium containing 10% foetal bovine serum, 100 UÆmL)1 penicillin, 1.0 mgÆmL)1 streptomycin and mM L-Glu For treating BxPC3 cells and clones with drugs affecting ... cloned into the corresponding sites of pEFneo, using the procedure described [20] Direct DNA sequencing of the construct obtained, performed by the dideoxynucleotide chain-termination method using ... 105 cells were plated in 12-well plates, incubated for 30 h with regular medium that was replaced with medium containing 1.0 lgÆmL)1 swainsonine (Sigma) or mM benzyl-a-GalNAc (Sigma) After growing...

Ngày tải lên: 19/02/2014, 12:20

... fused to d1-EGFP are maintained in the cells by binding to antigen Generation of soluble binding-defective and cysteine-deleted mutants To demonstrate that the observed intracellular colocalization ... was obtained with scFv 4A-d1-EGFP and 7A-d1-EGFP expressed in parallel Interestingly, in the case of 4A (but not 7A), we found a minor percentage of cells with a faint cytoplasmic staining (arrow) ... the 4A- or the 7A-cotransfected cells, thus demonstrating that strong in vivo binding of the destabilized 13R4-d1-EGFP proteins allows them to be maintained in the cells To rule out the possibility...

Ngày tải lên: 19/02/2014, 18:20