polycomb trithorax protein function and dna methylation fig 4 1

... Radom L J Am Chem Soc 19 99; 12 1: 9388–9399 Moore BS, Eisenberg R, Weber C, Bridges A J Am Chem Soc 19 95; 11 7: 11 285 11 2 91 Vlasie MD, Banerjee R Biochemistry 20 04; 43 (26): 8 41 0– 8 41 7 A3-038P Computer ... 2002; 46 : 611 –6 21 Antonyuk LP, Ignatov VV, Russ J Plant Physiol 20 01; 48 : 42 7 43 3 A1-034P Purification and some properties of glucose6-phosphate dehydrogenase from sheep kidney cortex B Tandogan and ... placenta protein 23 (PP23) / SOUL protein ´ ´ A Szigeti1, S Bellyei1, A Boronkai1, O Minik1, Z Szabo1, ´ ´ ´ Z Bognar1, K Komlosi2, R Ohmacht1, B Melegh2, T Janaky3, H Bohn4, B Sumegi1,5 and N Than1,6...

Ngày tải lên: 23/03/2014, 15:20

... 1. 8 3.5 1. 6 1. 0 1. 5 2.0 2.5 3.0 1 .4 1. 2 1. 0 10 0.8 0.6 12 11 10 Methylation level near start codon 11 .5 10 .5 8.5 9.0 9.5 Elongating Pol II density 40 00 11 0.50 10 0 .45 Elongating Pol II density ... 0.5 ~ kb 9.5 3.0 4. 0 10 .5 10 10 ~ kb 9.0 9 .4 9.8 3.0 4. 0 5.0 ~ kb ~ kb 9.5 10 .5 ~ kb 50 50 ~ 10 kb 8.5 1. 0 2.5 9.5 10 .5 4. 0 ~ 10 kb Both Methylation ( > or 0.5 ) None (

Ngày tải lên: 09/08/2014, 20:20

... DNA Damage and Repair 1. 2 Double Strand Breaks (DSBs) 1. 3 DNA Damaging Agents in Cancer Therapy 1 .4 Ku70/80 1. 5 DNA- PKcs 14 1. 6 Ku /DNA- PKcs ... 16 1. 7 DNA/ DNA-PK Interactions 19 1. 8 Downstream phosphorylation targets of DNA- PKcs 20 1. 9 Significance: 22 Materials and Methods 24 2 .1 Ku Mutant ... interactions with Ku and DNA in vivo and in vitro66 In order to better understand how protein/ protein and protein/ DNA interactions influence DNA- PK activation, we created and purified Ku80 C-terminal...

Ngày tải lên: 24/08/2014, 09:33

... R4 is presented in Figure 3-6 11 1 Figure B -1 Typical density of states (denoted here as ) for solid (a), gas (b) and liquid (c) (d) shows 2PT model (Figure adopted from [ 14 9 ] 11 6 Figure ... 10 8 6.2.3 Effect of Osmolytes on Protein- DNA Interaction 10 9 6.2 .4 Role of Phosphate Neutralization on DNA Conformation 11 0 APPENDIX A 1 14 APPENDIX B 11 7 ... entry 1ERI) with the DNA sequence d(CGCGAATTCGCG)2 [11 6, 11 7] Residues of Subunit I of the protein were numbered 1- 2 61 and the residues of Subunit II of the protein were numbered 2 74- 5 34 The...

Ngày tải lên: 09/09/2015, 18:49

... 0. 21 22.57 48 .10 71. 52 10 0.0 42 .83 32.70 24. 47 28 .12 20. 51 14 . 80 0 .42 28 .48 53.80 80.38 99.58 2.53 20. 04 49.36 73 .42 10 0.0 0. 21 21. 94 46 . 41 66 .46 98.95 35 .44 35 .44 29 .11 23 .47 23. 04 21. 35 0. 21 ... 47 .26 70.68 10 0.0 0 .42 27.00 54. 01 81. 22 99.58 1. 05 24. 47 48 . 31 75 .11 99.79 32.07 43 .46 24. 47 30.66 15 . 01 20.30 0 .42 28 .48 53 .16 76 .16 99.58 1. 27 23 .42 50.63 76.79 10 0.0 0. 21 21. 94 46 . 41 66 .46 ... O70397) 6. 54 2.95 4. 43 4. 01 4. 43 7.38 3 .16 6.33 5.06 8.02 1. 05 3 .16 7. 81 4. 22 5.06 7 .17 6. 54 6.96 1. 89 3.80 25.95 42 . 41 31. 65 23. 04 18 .39 25 .16 1 .48 23.00 47 .68 75 .11 99.79 0 .42 27.00 53 .16 79.96...

Ngày tải lên: 16/09/2015, 08:31

... CD2 -1- 6-3 Interaction PD Input GST CD2 -1- 1 CD2 -1- 2 CD2 -1- 3 CD2 -1 -4 CD2 -1 767 250 15 0 PD 7 34 Input GST CD2 -1- 5 CD2 -1- 6 CD2 -1- 7 CD2 -1- 8 CD2 -1 600 7 34 744 755 250 PD Input GST CD2 -1- 6 -1 CD2 -1- 6-2 ... CD2 -1 CD2-2 CD2-3 CD2 10 99 Inter- 600 250 767 15 0 9 34 IB: -v-KIND E F CD2 -1 CD2 -1- 1 6 34 CD2 -1- 2 667 CD2 -1- 3 7 01 CD2 -1 -4 CD2 -1- 5 735 CD2 -1- 6 702 CD2 -1- 7 668 CD2 -1- 8 635 CD2 -1- 6 -1 702 CD2 -1- 6-2 ... 16 51 16 61 ª 2 011 The Authors Journal compilation ª 2 011 FEBS 16 57 Core modules for the very-KIND-MAP2 interaction J Huang et al A EGFP v-KIND L474A C 14 12 *** 10 EGFP v-KIND L474A 20 50 80 11 0...

Ngày tải lên: 14/02/2014, 19:20

... transformation and cancer progression Oncol Rep 17 , 12 79 12 90 Itoh N, Yonehara S, Ishii A, Yonehara M, Mizushima S-I, Sameshima M, Hase A, Seto Y & Nagata S 11 2 11 3 1 14 11 5 11 6 11 7 11 8 11 9 12 0 12 1 12 2 12 3 ... (2008) 5 217 –5235 ª 2008 The Authors Journal compilation ª 2008 FEBS 52 31 DNA methylation- mediated nucleosome dynamics 98 99 10 0 10 1 10 2 10 3 1 04 10 5 10 6 10 7 10 8 10 9 11 0 11 1 5232 S K Patra and M Szyf ... JS, Fleisher TA, Stetler-Stevenson M & Strober W (19 92) A novel DNA methylation- mediated nucleosome dynamics 13 8 13 9 14 0 14 1 14 2 14 3 14 4 14 5 14 6 lymphoproliferative ⁄ autoimmune syndrome resembling...

Ngày tải lên: 18/02/2014, 14:20

... ± 1. 3 3 .4 ± 0 .4 6.8 ± 1. 2 0.9 ± 0 .4 41. 6 ± 1. 2 2.7 ± 0.3 3.0 ± 0.3 0.7 ± 0.3 42 .8 ± 13 .9 2.0 ± 0.2 3.8 ± 0.6 0.7 ± 0.2 13 .0 ± 3.9 2 .1 ± 0.3 4. 1 ± 0.8 0 .4 ± 0.2 61 ± 14 1. 5 ± 0.3 4. 2 ± 0.5 0 .13 ... (20 04) Biochemistry and biology of mammalian DNA methyltransferases Cell Mol Life Sci 61, 25 71 2587 24 Momparler RL & Bovenzi V (2000) DNA methylation and cancer J Cell Physiol 18 3, 14 5 1 54 25 ... and Moscow State University), NIH Grant CA 09 91 94 (New York University), and RFBR Grants 04- 04- 4 948 8 and 05- 044 9690 (Moscow State University) We thank Dr B Jack and Dr G Wilson from New England...

Ngày tải lên: 19/02/2014, 00:20

... pCR2 .1 ⁄ PDIP46 (1) ⁄ SKAR(a) pCR2 .1 ⁄ PDIP46(2) ⁄ SKAR(b) pHybLex ⁄ Zeo-ER pQE30 ⁄ ER-FLAG pGEX-4T1 ⁄ ER pGEX-4T1 ⁄ PDIP46 ⁄ SKAR(L7) pEGFP-N1 ⁄ ER pEGFP-N1 ⁄ PDIP46 (1) ⁄ SKAR(a) pEGFP-N1 ⁄ PDIP46(2) ... The pGEX-4T1 and pGEX4T1 ⁄ PDIP46 ⁄ SKAR(L7) plasmids were transformed into E coli BL 21( DE3) and expression of proteins was induced at A600 of 0.9 1. 0 (1 mm IPTG, h, 37 °C) The pGEX4T1 ⁄ ER plasmid ... EMBO J 17 , 6 649 –6659 Fingar DC, Salama S, Tsou C, Harlow E & Blenis J (2002) Mammalian cell size is controlled by mTOR and its downstream targets S6K1 and 4EBP1 ⁄ eIF4E Genes Dev 16 , 14 7 2– 14 8 7 Leighton...

Ngày tải lên: 19/02/2014, 05:20

... CRPW13F CRPW13F(cAMP)2 CRPW13F(cAMP )4 CRPW13FICAP CRPW13Flac CRPW13Fgal 5.83 5.59 5.99 5.55 5.85 5.85 4. 42 1. 55 1. 54 2 .10 2.23 2 .17 24. 2 72.3 74. 3 62.2 61. 9 62.9 ặRổ (A) ặEổ (%) 0.50 0. 31 ... used: 14 650 )1 )1 M ặcm at 259 nm for cAMP [16 ] and 6000 M )1 cm )1 at ể FEBS 2003 Conformational changes induced by cAMP and DNA binding to CRP (Eur J Biochem 270) 14 1 5 340 nm for AEDANS [18 ] Absorption ... of 15 .3 ns, detected for apo-CRP, to the value of 10 .4 ns for CRP(cAMP)2 complex The decrease in rotational time 0.28 0 .11 0 .10 0.06 0.06 0.25 8 .1 2.5 2 .4 2.0 1. 5 4. 3 26.6 18 .7 18 .4...

Ngày tải lên: 20/02/2014, 23:20

... AtTic32-IVa (At4g2 343 0) AtTic32-IVb (At4g2 342 0) AtTic32-IVc (At4g1 14 1 0) AtTic22-IV (At4g33350) AtTic22-III (At3g23 710 ) AtTic 21/ AtPIC1 (At2g15290) AtTic20-I (At1g 049 40) AtTic20-IV (At4g03320) AtTic20-V ... regulation [10 15 ,28,33,57] [47 , 51, 53] [48 ,49 ,58] [27,28,32, 34, 35,59,60] Intermembrane space complex (with Toc12, imsHsp70 and Toc 64) Channel protein; Fe-permease [ 21, 62– 64] Channel protein [19 – 21, 65] ... Arabidopsis isoforms, and the proposed function Tic component Isoforms in A thaliana (AGI) Tic 110 Tic62 Tic55 Tic40 AtTic 110 (At1g06950) AtTic62 (At3g18890) AtTic55 (At2g 248 20) AtTic40 (At5g16620) Tic32...

Ngày tải lên: 07/03/2014, 03:20

... involved in DNA methylation Figure represents average ModellingMethylation Dynamics Modelling DNA DNA Methylation Dynamics S.No 10 11 12 13 14 15 16 17 18 19 13 11 Genes PRSS7 IFNGR2 KCNE1 MRAP IFNAR2 ... H1 zero H2A Four S1-R3-K5-K9 Ph-Met-Ace-Ace H2B Ten K5-S10-K 11- K12 Ace/Met-Ph-Ace-Ace S 14 - K15-K16 Ph-Ace-Ace K20-K23-K 24 Ace-Met-Ace *H3 Six R2-T3-K4 Met-Ph-Met R8-K9-S10 Met-Ace/Met-Ph T 11- K 14 - R17 ... modifications, associated with DNA methylation and tumor progress are – acetylation of H3K18, H4K16 and H4K12, trimethylation of H3K4 and H4K20, acetylation/trimethylation of H3K9, trimethylation of H3K27,...

Ngày tải lên: 08/03/2014, 19:20

... at 13 0 and 15 0 mM salt Naked DNA (lanes 4 7) and chromatin assembled in the absence or presence of R3 protein are shown R3 was added to lanes 1, 4, and 2398 FEBS Journal 2 74 (2007) 2396– 2 41 0 ... between L1 and L2 is 18 3 bp, and L3 is at a distance of 14 0 bp from L2 The plasmids had unique sites for the restriction enzymes: AlwN1 at 709 bp from the third lac operator, L3; and Xmn1 11 17 bp ... nucleosomes mark the 2 41 0 39 40 41 42 43 44 45 gene boundaries of the PHO5 ⁄ PHO3 locus in yeast EMBO J 5, 26 81 2687 Perez-Ortin JE, Estruch F, Matallana E & France L (19 87) Fine analysis of...

Ngày tải lên: 16/03/2014, 10:20

... bottom bands) The fragment was nonplatinated (control) (lane 1) or modified by trans-PtHMP at rb ¼ 7.5 · 10 )4, · 10 )4, 2.5 · 10 )4 or · 10 )4 (lanes 3–6, respectively); and by cisplatin at rb ¼ · 10 )3 ... Cisplatin Transplatin c 12 0 6d 10 2 64 90 12 70 Decrease 28° Increaseh 13 ° i Decreaseh 9°i 1. 7 °C ) 2.0 °C 0.6 °C f 12 0 12 d c 300 26 40 f g 48 l g l adducts disturb DNA conformation much more ... the 1, 3-intrastrand CLs of parent transplatin was markedly higher, after 24 h 70% of the 1, 3-intrastrand CLs were transformed into the interstrand CLs [ 34] FEBS Journal 273 (2006) 3 01 3 14 ª...

Ngày tải lên: 16/03/2014, 14:20

... PsaE Lhca1 Lhca2 Lhca3 Lhca4 896 3h 6h 3h 6h 3h 6h 10 0 10 0 10 0 10 0 10 0 10 0 10 0 68 10 6 36 71 45 62 74 72 0 52 10 5 10 3 73 91 54 83 83 10 9 10 3 55 77 38 37 65 55 10 6 95 97 63 93 91 45 10 9 86 10 6 58 ... 37 38 39 40 41 42 43 44 45 46 photosystem I by NADP-dependent reversible conformational changes Biochemistry 42 , 11 839 11 845 Scheller HV, Jensen PE, Haldrup A, Lunde C & Knoetzel J (20 01) Role ... Physiol 10 7, 1 049 10 54 24 Foyer CH, Lelandais M & Kunert KJ (19 94) Photooxidative stress in plants Physiol Plant 92, 696– 717 25 Vass I (19 97) Adverse effects of UV-B light on the structure and function...

Ngày tải lên: 16/03/2014, 18:20

... emerging concept for G protein- coupled 2 944 12 13 14 15 receptor ontogeny and function Annu Rev Pharmacol Toxicol 42 , 40 9 43 5 Jordan BA, Trapaidze N, Gomes I, Nivarthi R & Devi LA (20 01) Oligomerization ... receptor function Nature 399, 697–700 39 Cheng ZJ, Harikumar KG, Holicky EL & Miller LJ (2003) Heterodimerization of type A and B 2 945 Function and pharmacology of hetero-oligomers 40 41 42 43 44 cholecystokinin ... adrenergic a1a FEBS Journal 272 (2005) 2939–2 946 ª 2005 FEBS Function and pharmacology of hetero-oligomers and a1b receptors [ 21] , neurokinin NK1 and l opioid receptors [22], and the b2-adrenergic and...

Ngày tải lên: 16/03/2014, 22:20

Ngày tải lên: 23/03/2014, 03:20

... E2a-Pbx1 Proc Natl Acad Sci USA 94, 14 5 53– 14 5 58 44 Goutte C & Johnson AD (19 94) Recognition of a DNA operator by a dimer composed of two different homeodomain proteins EMBO J 13 , 14 3 4– 14 4 2 202 ... 270, 311 78– 311 88 43 Knoepfler PS, Calvo KR, Chen H, Antonarakis SE & Kamps MP (19 97) Meis1 and pKnox1 bind DNA cooperatively with Pbx1 utilizing an interaction surface disrupted in oncoprotein ... two classes, I and II, by sequence relatedness and expression patterns [10 ] Based on the expression patterns [11 13 ], analysis of mutants [ 14 17 ] and overexpression studies [18 – 21] it was proposed...

Ngày tải lên: 23/03/2014, 13:20

... Acad Sci 2002; 9 61: 292–297; 2003; 9 91: 333–338; 20 04; 10 22 :16 3 1 84; FASEB J 20 04; 18 : C62; 2005; 19 : 360.3 & 19 6 .4; Mol Biol Cell Suppl 20 04; 15 : 47 9a 48 0a & L 312 ; Biophys J 2005; 84: 2000] Thus, ... 2000; 84: 543 5– 543 8 Edler J, Hamm P J Chem Phys 20 01; 11 7: 2 41 5– 242 4 N1-055P Oligopeptide repeats of Sup35 as the determinants of [PSI+] variability I S Shkundina1, V V Kushnirov1, M F Tuite2 and ... (projects Nos 04- 04- 48368, 05- 044 8 319 , 03- 04- 48562) N1-027P Modification of tryptophan residues with trihalocompounds and its application to protein chemistry C L Ladner, R J Turner and R A Edwards...

Ngày tải lên: 23/03/2014, 15:20

... 10 )10 SOUL P22HBP Sw Mb 1. 9 · 10 6 1. 0 · 10 8 7.6 · 10 7 6 .1 · 10 )3 4. 4 · 10 )3 8 .4 · 10 )7 3.2 · 10 )9 4. 4 · 10 )11 1. 3 · 10 ) 14 Unpublished observationsb [17 ] [17 ] [18 ] 40 35 Proteins 0 0.5 40 1. 0 ... 55x10 60 50 45 koff (s )1) Kd (M) 3.3 · 10 7 < 10 5 7.7 · 10 5 < 10 5 1. 1 · 10 7 5.3 · 10 )3 1. 6 · 10 )10 3.2 · 10 )3 3.0 · 10 )4 1. 5 · 10 )3 This work This work [ 21] HRI Absorbance kon (mol )1 s )1) 1 .4 · 10 )10 ... bHLH-PAS-A of NPAS2 A 1 648 15 84 16 06 15 08 Intensity 585 5 14 523 348 Intensity 13 61 1376 1 642 675 16 70 B 300 600 500 40 0 Raman Shift (cm 1 ) 700 13 00 15 00 16 00 14 0 0 Raman Shift (cm 1) 17 00 Fig Effects...

Ngày tải lên: 30/03/2014, 11:20