physiological and biochemical responses in plant cells during drought stress

... chloride, and betaine concentrations in isolated vacuoles from salt-grown Atriplex gmelini leaves Plant Physiol 84, 173–177 McKersie, B.D., Leshem, Y.Y., 1994 Stress and Stress Coping in Cultivated Plants ... mangrove in Okinawa, Japan Materials and methods 2.1 Source of plants and growth Seeds of B gymnorrhiza were collected from Iriomote Island, Okinawa, Japan, at the end of May in 1997 and June in 1999 ... were planted in each culture pot (15 cm in diameter and 14 cm in height) with vermiculite The pots were irrigated every days with water containing 0, 125, 250 and 500 mM NaCl Salt concentration in...

Ngày tải lên: 06/07/2014, 02:20

... sugars and sugar metabolizing enzymes in both sugar and ROS signalling pathways and their Sugar signalling and antioxidant networks in plants co-operation with antioxidant networks in plant cells ... signalling pathways [2] Glucose controls abscisic acid (ABA) signalling and biosynthesis gene expression [41] and HXK-dependent signalling interacts positively and negatively with auxin and cytokinin ... alterations in mitochondrial movements and morphology, and cell death [132], similar to that observed in animal cells [131], suggesting that similar mechanisms might be operating in plant and animal cells, ...

Ngày tải lên: 15/03/2014, 11:20

... shown in a previously published in vitro study [36] In contrast, in our present in vivo study, both DCTs and DCTIs were comparable in inducing peptide-specific T cell responses, following vaccination ... partial clinical responses following vaccination with the vaccination time point Patient PSA baseline levels (μg/L) Vaccination time point (V) PSA Post-response (μg/L) Vaccination time point (V) ... between clinical responses and T regs in cancer patients undergoing hTERT-pulsed DC vaccination This novel finding is of substantial significance in hTERT-based immunotherapy in particular, and cancer...

Ngày tải lên: 18/06/2014, 15:20

... -15b and let-7f were downregulated in effector cells compare to naïve cells and increased back in memory T cells [14] Effector T cells originate from naïve T cells following antigen exposure and ... role in innate and acquired immune response Elucidating the mechanisms by which miR-155 expression is controlled and finding its targets will bring more understanding of the mechanisms of tumor induction ... miR-150, is mainly expressed in the lymph nodes and spleen and is highly up-regulated during the differentiation of mature T and B cells suggesting that it may participate in B and/ or T lymphopoiesis...

Ngày tải lên: 08/08/2014, 17:20

... callose Biochemical studies focused on pollinic and stigmatic proteins, known to be involved in male-female interactions (Gaude and Dumas, 1987) After silver nitrate staining, qualitative and quantitative ... nigra and P alba pollen tubes inside stigmatic tissues However, increasing protein bands were detectable, 0-20 h after pollination, only in compatible pollinated stigmas The concanavalin A-peroxidase ... Identification of concanavalin A-binding proteins after sodium dodecyl sulfate-gel electrophoresis and protein blotting Hawkes R AnaL Biochem 23, 143-146 Singh K and Knox R.B (1985) P-Galactosidase...

Ngày tải lên: 09/08/2014, 04:20

... and in control and in minor amount in fraction 7, while in hypoxia almost doubled in fractions and Lipid analysis in DRF Cholesterol was enriched in DRF in control (878 ± 80, nmol/mg prot) and ... in control and treated lungs, averaging ~3% of total phospholipids In PMC the amount of choline plasmalogen (included in PC) and ethanolamine plasmalogen (included in PE) were 0.024 and 0.16 nmoles/mg ... increase in basement membrane thickness similar to that occurring in hypoxia, there was a consider- able increase in cell volume and, particularly for endothelial cells, in surface area and in...

Ngày tải lên: 12/08/2014, 16:20

... and ALT = 41 IU/L), leading to lamivudine interruption at M30 because, at that time, the benefit of maintaining dual therapy was not proven With adefovir Figure HBV viral load and alanine aminotransferases ... line probe assay (InnoLipa-DR2-assay, InnoGenetics, Ghent, Belgium), following the manufacturer's instructions This second genotyping assay was performed because of its sensitivity in detecting ... concomitantly detected with sequencing and line probe assays from M6 to M39 (9 months after cessation of lamivudine) During this period, the rate of rtM204V/I strain remained stable, as shown by the...

Ngày tải lên: 13/08/2014, 13:20

Ngày tải lên: 13/11/2014, 09:42

... examined the bovine sperm-binding activity of heterospecific bovine ⁄ porcine rZP3 ⁄ rZP4 mixtures Both porcine rZP3 ⁄ bovine rZP4 and bovine rZP3 ⁄ porcine rZP4 inhibited bovine sperm–ZP binding at a ... chains of mouse ZP in sperm binding [27–29] In pigs, neutral tri-antennary and tetra-antennary complex-type chains have the strongest sperm-binding activity of the N-linked chains of ZP [30], and ... glycopeptidase F, indicating that rZP2 and rZP4 have N-linked chains (Fig 7A), the resulting bands were not sufficiently resolved to deduce the number of N-linked chains in these proteins Native bovine ZP2...

Ngày tải lên: 07/03/2014, 05:20

... membrane expansion and remodeling In most instances, there is coordinate expression of the E1 and E2 components The transient accumulation of mRNA preceding changes in protein levels and catalytic ... among dicot plants In a detailed study of pea (Pisum sativum) seedling development, it was observed that changes in the levels of E1 and E2 proteins and mRNAs were coordinated with changes in mtPDC ... were found in cells or organs that were rapidly expanding or differentiating; etiolated seedlings or the youngest leaves of light-grown plants Activities decreased in mature leaves and were virtually...

Ngày tải lên: 17/03/2014, 09:20

... suspensions were obtained in 0.2% methylene blue, incubated for min, and viable (unstained) cells and dead (stained cells) were counted in Neubauer hemocytometer chambers Bioinformatics and statistical ... domain The N- and C-termini are least conserved in all proteins In particular, N-termini are highly heterogeneous, accounting for variability in total protein lengths ranging from 124 to 1057 amino ... preincubation in 10 lm actinomycin D Even lm actinomycin was sufficient to effectively block transcription in mIMCD3 cells (Fig S2) Cells were then exposed to hyperosmotic stress, aldosterone, and...

Ngày tải lên: 30/03/2014, 10:20

... K Rathinasamy and D Panda [10] The binding site of benomyl in tubulin is yet to be determined However, it has been suggested that benomyl binds to tubulin at a site that is distinct from ... tubulin at a site that is distinct from the colchicine and vinbastine binding sites in tubulin [10,11] Although benomyl inhibits mitosis in mammalian cells, its antimitotic mechanism of action is ... the motor proteins and spindle checkpoint proteins [19] This reduction of checkpoint proteins is required for inactivating the spindle checkpoint signal and progression in the cell cycle [20] The...

Ngày tải lên: 30/03/2014, 10:20

... control the oxidant generated and remain the oxidant-antioxidant balance, which was important in maintaining the cell membrane integrity and functionality, cell proteins and nucleic acids (Knight, ... antioxidants in diet were mainly from plant origins, such as fruits, vegetables and herbs Various studies were conducted to determine the antioxidant activity in plants to detect the plants which ... REVIEW 2.1 The herbal industry Herbal medicine could be used as part of the traditional medicinal practices and it had long history since ancient including Traditional Chinese Medicine, Traditional...

Ngày tải lên: 05/05/2014, 08:39

... Boehringer M, Hull M: Interleukin-1beta induces cyclooxygenase-2 and prostaglandin E(2) synthesis in human neuroblastoma cells: involvement of p38 mitogenactivated protein kinase and nuclear factor-kappaB ... Neuroinflammation 2005, 2:27 http://www.jneuroinflammation.com/content/2/1/27 Findings Aberrant expression of cyclins, cyclin dependent kinases (CDKs) and their inhibitors has been observed in ... emerging data on the early role of oligomeric and protofibrilic forms of Aβ in AD is very interesting [25,26] Whether COX-2 and cell cycle proteins are part of the molecular mechanisms involved in...

Ngày tải lên: 19/06/2014, 22:20

... needles in dormant and growing seedlings before planting (0) and after 1-, 2-, 3- and 4-week postplanting drought periods Vertical bars indicate the standard error of the mean In Ψ shoot pd and Ψshoot ... in July in central Finland Shoot xylem water potential (Ψshoot) and needle chlorophyll fluorescence of dormant and growing seedlings were studied before planting and after four postplanting drought ... (root egress), and (b) initial heights, height growth of growing seedlings before planting and height growth of dormant and growing seedlings, when exposed to different postplanting drought periods...

Ngày tải lên: 08/08/2014, 00:21

... in RA and in OA, with stronger NF-κB dependence observed in RA [[38,43,44] versus [28]] The third major finding in this study Figure and methods and reaction data from link protein, chain in Figures ... player in promoting the production of inflammatory and degradative mediators in the OA synovium In particular, our results indicate a role for both TNF-α and IL-1β in driving inflammatory responses ... Figures and derived as described in Materials Polymerase and ADAMTS analysis,7 matrix metalloproteinase (MMP), link protein, and ADAMTS analysis, derived as described in Materials and methods and in...

Ngày tải lên: 09/08/2014, 08:23

... temperature in streptavidin–peroxidase complex (1:500 in DPBS; Dako), rinsed in distilled water and incubated in fast-DAB (3,3'-diaminobenzidine) peroxidase substrate (Sigma) for minutes and counterstained ... antibody (green), and 4',6-diamidino-2-phenylindole (blue) Results showed strong staining for CD13, CD29, CD44, CD90 and CD105, weak staining for 3G5, and negative staining for low-affinity nerve growth ... cells) , and for CD29 (β1 integrin) The cells stained poorly for LNGFR and STRO-1 (markers on freshly isolated bone marrow stem cells) and sparsely for 3G5 (marker for vascular pericytes) Staining...

Ngày tải lên: 09/08/2014, 10:20

... formation and TFIIB binding in vivo The high prevalence for the occurrence of motif combinations in TFIIB-dominated genes in illustrated in Figure 5e In line with the known synergy between INR and ... (black line) and replicate (grey line) relative to aligned TSSs at 10-bp resolution Binding of TBP and the initiating form of RNAPII (phosphorylated at serine in its carboxy-terminal domain) were ... one initial cycle consisting of minutes at 55° (during which polymerase was added), minutes at 72°C and minutes at 94°C, followed by 22 cycles of 0.5 minutes at 94°C, 0.5 minutes at 60°C and minutes...

Ngày tải lên: 09/08/2014, 20:21

... in the F protein led to an apical redistribution in endothelial cells whereas only tyrosine 525 is involved in targeting in epithelial cells Neither the di-tyrosine nor the di-leucine motif in ... F and G proteins were separated by SDS-PAGE and blotted to Table Summary and comparison of NiV infection and glycoprotein targeting in polarized epithelial and endothelial cells Epithelial cells ... basolateral sorting motifs, a di-tyrosine motif in the F protein (Y542/ 543) and a di-leucine motif in the G protein (L41/42) had no influence on basolateral sorting (table 1) Epithelial and endothelial...

Ngày tải lên: 12/08/2014, 02:20