phan mem giam sat hoat dong luoi dien
Báo cáo y học: "Post-translational aging of proteins in osteoarthritic cartilage and synovial fluid as measured by isomerized aspartate" pot
Ngày tải lên :
09/08/2014, 14:20
... Arnold & Co; 1949 Richardson CD, Bae WC, Fazeli B, Filvaroff EH, Sah RL: Quantitative characterisation of osteoarthritis in the guinea pig Paper presented at: Orthopaedic Research Society 47th...
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Post translational regulation of the human TRIP br1 cell cycle protein
Ngày tải lên :
14/09/2015, 13:56
... School of Medicine, NUS 1999-2007 FIGURE Domain organization of members of the E2F family [38] The identified domains within E2F family members are shown in the schematic above, namely, cA, Cyclin ... Loo Lin School of Medicine, NUS 1999-2007 TABLE E2F family members classified according to functional and physical characteristics Group Members E2F1, E2F2 & E2F3 E2F4 & E2F5 E2F6 E2F7 Characteristic ... is primarily compartmentalized into the nucleus and the cytoplasm by an intracellular membrane, the nuclear membrane, which has selectivity in the import and export of proteins There are nuclear...
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Tài liệu Báo cáo khoa học: Complex transcriptional and translational regulation of iPLA2c resulting in multiple gene products containing dual competing sites for mitochondrial or peroxisomal localization docx
Ngày tải lên :
19/02/2014, 16:20
... lysolipids alter the physical properties of cell membranes, interact with specific receptors, and modulate the electrophysiologic function of many transmembrane ion channels including K+ and Ca2+ channels ... despite our intense efforts at solubilization and purification, the membrane-associated iPLA2 activities we identified in multiple membrane compartments were resistant to our attempts at their purification ... removal of the supernatant, the membrane pellet was resuspended in mL of homogenization buffer using a Teflon homogenizer and then sonicated (5 · s bursts) to prepare a membrane fraction PLA2 enzymatic...
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Tài liệu Báo cáo khoa học: Post-translational cleavage of recombinantly expressed nitrilase from Rhodococcus rhodochrous J1 yields a stable, active helical form ppt
Ngày tải lên :
19/02/2014, 00:20
... classification of isolated particle images (C, D) Putative top and side views of ‘c’-shaped class members and the corresponding class average are shown The length of the ‘c’ varied between and 13 ... interpretation of the map required the creation of a model of the J1 nitrilase based on its homology with members of the superfamily for which crystal structures exist (Fig 6) Alignment of the sequence ... between the subunits occur at the surfaces marked A, C and D The A surface is preserved in all members of the nitrilase superfamily whose structures have been determined crystallographically...
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Báo cáo Y học: Regulation of RAS in human platelets Evidence that activation of RAS is not sufficient to lead to ERK1-2 phosphorylation pot
Ngày tải lên :
08/03/2014, 16:20
... Lysed cells and debris were removed by centrifugation Cell lysates were precleared for h at °C with protein A–Sepharose Platelet lysates were incubated overnight at °C with lL anti-GRB2 or anti-SHC ... Lysed cells and debris were removed by centrifugation Cell lysates were precleared for h at °C with glutathione–agarose Platelet lysates were incubated h at °C with lg GST–RAF–RBD immobilized ... centrifugation Laemmli buffer was added, and the lysate was boiled for Proteins were separated by SDS/PAGE and transferred to poly(vinylidene difluoride) (PVDF) membrane Blots were developed by using the...
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Báo cáo khoa học: Factor-inhibiting hypoxia-inducible factor (FIH) catalyses the post-translational hydroxylation of histidinyl residues within ankyrin repeat domains pot
Ngày tải lên :
28/03/2014, 23:20
... FIH are limited to asparaginyl and in one case, ankyrinR, an aspartyl residue [14] Tankyrase is a member of the poly-ADP-ribose polymerase family, which uses NAD+ as a cosubstrate to link ADP-ribose ... oxygen 1088 species are also proposed to enable self-hydroxylation of FIH at an active-site tryptophan residue [18] To investigate the regiochemistry of the FIH-catalysed His hydroxylation, we analysed ... and micro-organisms, they catalyse a much wider range of reactions, including hydroxylation and desaturation (e.g such as occurs in flavonoid biosynthesis) [22,23] The observation that FIH can catalyse...
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Báo cáo sinh học: "Regulation of metabolism in Caenorhabditis elegans longevity" ppsx
Ngày tải lên :
06/08/2014, 19:21
... pathways in C. elegans longevity Black lines indicate the activating or repressive function of members of different signal transduction pathways Amino-acid pools and other nutrients activate ... February 2010 References Morris JZ, Tissenbaum HA, Ruvkun G: A phosphatidylinositol-3-OH kinase family member regulating longevity and diapause in Caenorhabditis elegans Nature 1996, 382:536-539 Kimura...
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Báo cáo y học: "Expression and regulation of CCL18 in synovial fluid neutrophils of patients with rheumatoid arthritis" potx
Ngày tải lên :
09/08/2014, 10:21
... were added to a final volume of 500 μl of DMEM/10% FCS Additionally PMN and EA-hy.926 cells were cultured in Boyden chambers, separated by a transwell membrane with a pore size of 0.4 μm (Corning ... Hanau, Germany) The pellet was resuspended in PBS, overlaid on an isotonic discontinous Percoll gradient (Amersham Biosciences, Freiburg, Germany) with densities of 1.075 g/ml and 1.09 g/ ml and ... Bartholomew's and the Royal London School of Medicine, London) were plated in 24-well plates in DMEM with 4,500 mg/l glucose, L-glutamine, sodium bicarbonate and pyridoxine hydrochloride (Sigma)...
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Báo cáo y học: "Expression and regulation of neurotrophins in the nondegenerate and degenerate human intervertebral disc" pdf
Ngày tải lên :
09/08/2014, 11:20
... 10 minutes The cell pellet was washed twice with DMEM and Ham's F-12 Cells were seeded in vented culture flasks (BD Falcon) and were fed with DMEM (glucose and pyruvate) supplemented with 10% ... extraction For cell extraction, NP tissue samples were minced and digested with U/ml protease in DMEM and Ham's F-12 media for 30 minutes at 37°C To isolate NP cells, tissue samples were treated ... within the central and peripheral nervous systems, and they consist of a family of four principal members: NGF, BDNF, neurotrophin and neurotrophin 4/5 Both NGF and BDNF have been identified in...
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Cyclic nucleotides and mitogen-activated protein kinases: regulation of simvastatin in platelet activation pptx
Ngày tải lên :
10/08/2014, 05:21
... (NSC97-2320-B-038-016-MY3) and Hsinchu Mackay Memorial Hospital (MMH-HB-96-02; MMH-HB-97-01) Author Details 1Department of Surgery, Hsinchu Mackay Memorial Hospital, Hsinchu; Mackay Medicine, ... (Beverly, MA); the anti-α-tubulin mAb was from NeoMarkers (Fremont, CA); and the Hybond-P PVDF membrane, ECL Western blotting detection reagent and analysis system, horseradish peroxidase (HRP)-conjugated ... NaCl, and 0.01% Tween 20) containing 5% BSA for h and then probed with various primary antibodies Membranes were incubated with HRP-linked anti-mouse IgG or antirabbit IgG (diluted 1: 3000 in TBST)...
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Báo cáo y học: "Stanniocalcin-1 promotes tumor angiogenesis through up-regulation of VEGF in gastric cancer cells" ppt
Ngày tải lên :
10/08/2014, 05:21
... (C) Immunohistochemical staining of STC-1 in tumor tissues of nude mice STC-1 was detected on the membrane of tumor cells (D) Immunohistochemical staining of PCNA in tumor tissues of nude mice PCNA ... avtivation induced by STC-1 BGC823 were treated with 50 ng/mL STC-1 for 15, 30, 45, 60 Whole- cell lysates were prepared and immunoblotted with antibodies to phosphor-PKC bII, total PKC bII, phosho-ERK1/2 ... activation induced by STC-1 BGC823 were treated with different concentrations STC-1 for 45 Whole- cell lysates were prepared and immunoblotted with antibodies to phosphorPKC bII, total PKC bII, phosho-P38...
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Báo cáo y học: " Post-translational modifications of histones H3 and H4 associated with the histone methyltransferases Suv39h1 and G9a" pdf
Ngày tải lên :
14/08/2014, 08:20
... (a) Purification of crude nucleosomal histones Nucleosomal histones were separated on a 4-12% gradient NuPAGE gel and run in MES buffer (Invitrogen), fixed, and stained with Seeblue (Invitrogen) ... study modifications of crude histone preparations, especially those of histones H3 and H4, gave satisfactory and informative results Consequently, we used this protocol to study the histone code ... observe good stoichiometry of the Suv39h1-associated proteins strongly bound to chromatin, such as members of the HP1 protein family, histones H1-H5, and macro-H2A (Figure 2b) The double immunopurification...
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Báo cáo y học: "Global nucleosome distribution and the regulation of transcription in yeas" doc
Ngày tải lên :
14/08/2014, 14:21
... chromatin Proc Natl Acad Sci USA 2004, 101:1525-1530 Belotserkovskaya R, Oh S, Bondarenko VA, Orphanides G, Studitsky VM, Reinberg D: FACT facilitates transcription-dependent nucleosome alteration...
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Báo cáo y học: "Translational regulation of gene expression" docx
Ngày tải lên :
14/08/2014, 14:21
... development but also for regulation of ‘synaptic memory’ in the mammalian central nervous system Long-term synaptic plasticity and long-term memory require the synthesis of new proteins for their ... structural data on the ribosome and its associated complexes presented during the meeting led the audience to an appreciation of the ribosome as a dynamic machine whose contortions are subject to ... initiation An example of regulation of cap-dependent translation in synaptic plasticity and long-term memory was presented by Jessica Banko from Eric Klann’s laboratory (Baylor College of Medicine,...
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REGULATION OF CHEMICALS IN EUROPE – REACH
Ngày tải lên :
16/04/2015, 09:05
... commerce 100.000 TSCA compounds 82.000 Food additive 8.600 Cosmetic ingredients 3.400 Pharmaceuticals 1.800 Pesticides ingredients 1.000 USA EPA 2010 PRIORITY SETTING Tier Production volume (HPV) ... scenarios Chemical properties Bioavailability EU REGULATION OF CHEMICALS Registration Evaluation Authorisation CHemicals BEFORE 2008- EC 793/93 Existing chemicals - substances on the market 010171180981 ... data sharing Better information on hazards and risks Evaluation - ECHA (Helsinki) Authorisation – risk adequately controlled, socioeconomic benefits outweigh risk, no suitable alternative...
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Understanding the regulation of cytokinesis in fission yeast
Ngày tải lên :
11/09/2015, 09:06
... drive membrane assembly and invagination A GTPase-driven signaling cascade, septation initiation network (SIN), is activated to coordinate actomyosin ring constriction, cell wall and new membrane ... sufficient cell mass in G2 phase, cells enter mitosis, as characterized by chromosome condensation, nuclear membrane disassembly (in “open” mitosis of animal cells), and assembly of a mitotic spindle ... segregation, the actomyosin ring constricts and drives the invagination of plasma membranes in conjunction with assembly of new membrane and cell wall to form a barrier to generate two newly-born cells...
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Role and regulation of juxtanodin in actin cytoskeleton of oligodendrocyte
Ngày tải lên :
11/09/2015, 10:18
... Dr Tran Manh Hung, Dr Tang Junhong, Mr Xia Wenhao, Ms Wu Chun, Ms Pooneh Memar Ardestani, Ms Guo Jing and Ms Wang Xie The members in our group are warm-hearted and diligent It is my great honor ... DAPI 4’,6-diamidino-2-phenylindole DMEM dulbecco’s modified Eagle’s medium xi List of abbreviations DMSO dimethyl sulfoxide DNA deoxyribonucleic acid DRF diaphanous-related forming DTT Dithiothreitol ... at peripheral submembranous regions and the leading edges of processes (Simpson and Armstrong, 1999), where it interact with MF and may allow the local MF protruding the plasma membrane during...
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Regulation of cytokinesis in the fission yeast schizosaccharomyces pombe
Ngày tải lên :
14/09/2015, 14:18
... would like to thank my thesis committee members Drs Naweed Naqvi, Uttam Surana and William Chia for their comments and suggestions I would like to thank all the members past and present of the yeast ... animal cells Animal cells divide by invagination of the cell membrane, forming a cleavage furrow that gradually constricts generating a membrane barrier between the cytoplasmic constituents of each ... constrict the cleavage furrow from the plasma membrane inwards, plant cells divide by construction of cell plates sandwiched between new plasma membrane on either side (reviewed by Jurgens 2006)...
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