ph adjustment of liquid and agar growth media

... amounts of starting material and making the monitoring of expression of thousands of genes simultaneously possible Second, the increasing knowledge of DNA sequences of a large number of genes and ... Examples of staining of single neurons by intracellular iontophoresis of horseradish peroxidase Purkinje cells of the cerebellar cortex of the cat A: Reconstruction of the collateral branches of the ... spinal cord of both normal and mutant lines of zebrafish.” (Fetcho and O'Malley, 1995) Widespread use of tissue slices and of the confocal microscope, together with continued development of the applications...

Ngày tải lên: 11/04/2014, 09:51

... of 1,101 fulllength NA sequences of the subtypes, of a range of host species and from different geographical locations were retrieved and aligned using Biological Sequence Alignment editor software ... cause of morbidity and mortality in humans and in a wide range of animal species [1-3] Type "A" viruses are the most important pathogens of the three types and have been associated with all of the ... evaluation of the one-step RT-PCR assay and sequencing for N subtyping of influenza at AAHL, helped to draft and edit the manuscript, and participated in the overview of the study RB conceived of the...

Ngày tải lên: 20/06/2014, 01:20

... (CG) BamH I restriction site (GGATCC) Complement of stop codon (CTA) Overlap with the strand complement to the 3'-end of the coaD gene Estimated Tm of 60°C [8 * (A+T) + 11 * (C+G)] GC content is ... second triplet begins with a C and PCR amplification will change this codon from CAA to GAA (and the second residue in the recombinant protein will be glutamate instead of glutamine) An alternative ... 5'-end primer will not result in the amplification of the original coaD gene The use of the Nco I restriction site dictates what the first nucleotide of the next triplet codon must be (G) In the coaD...

Ngày tải lên: 14/07/2015, 23:53

... strains of Japanese encephalitis virus, 28 strains of St Louis encephalitis virus, 172 strains of West Nile virus and 24 strains of yellow fever virus using BLASTn [22] The genome sequences of the ... detect into five groups of primer pairs (Table and Tables S1 and S2) Here, we report the preparation and testing of a mixture of 10, 18- to 22-nucleotide PCR primers, each of which is at least two ... amplification of human DNA was observed with any of the primers Figure shows agarose gel electrophoresis of the amplification products; identical amplicons were observed in the presence and absence of excess...

Ngày tải lên: 14/02/2014, 19:20

... consisted of 25 cycles of 94 °C for 30 s, 66 °C for 30 s and 72 °C for The final extension step consisted of 72 °C for Ten microliters of the PCR product was checked by 1.2% agarose gel electrophoresis ... serial analysis of gene expression (SAGE) characterization of orphan SAGE tags from human embryonic stem cells identifies the presence of novel transcripts and antisense transcription of key pluripotency ... were found between the site of the no tag D tag and a poly(dA) among the cDNA of the gene (Hs 436980) The blast results of another 11 sequences in the GenBank sequences of the products If the tag...

Ngày tải lên: 07/03/2014, 04:20

... yield (expressed as quotient of the number of molecules of PCR product and the initial number of target molecules), n is the number of cycles, and ε is the mean efficiency of all cycles with ≤ ε ≤ ... Relief of inhibition by meat, blood, and feces Relief of inhibition by bilirubin and humics Relief of inhibition by melanin in RT-PCR Relief of inhibition by meat, blood, and feces Relief of inhibition ... bilirubin and humics Rescue of failed amplification Facilitation of RT-PCR Relief of inhibition by feces Relief of inhibition by plant polysaccharides Relief of inhibition by blood and meat PCR of GC-rich...

Ngày tải lên: 11/04/2014, 10:01

... determining the suitability of 10 commonly used reference genes in the context of infection with various strains of HIV-1, HSV-1, CMV and VZV in a range of both primary and cultured cell lines using ... VZV strain Schenke and HIV-1 strains BaL, NL4.3 and RFW were propagated according to standard protocols [16-20] The cell types infected, MOI, time of infection and percentage of cells infected ... strain), CMV (Toledo and Towne strains) and VZV (Schenke strain) and harvested at the earliest time of maximal productive infection as illustrated in Table The expression levels of 10 commonly used...

Ngày tải lên: 18/06/2014, 18:20

... thiocyanate and phenol/chloroform extraction (R2V1) (compare lanes and versus and 4) Random hexamers (Rndm) were more efficient than B4R1, a primer specific to the 3'-UTR (lanes and versus and 6, ... and versus and 6, or lanes and versus and 10) For amplicon 2, AMVRT and M-MLV RT worked equally well (lanes and versus and 8, or lanes and versus and 10) Lanes 11 and 12 are negative controls ... by agarose gel electrophoresis comparing band intensity to the Hyperladder I (Bioline) marker Two sets of DNA sequencing primers were designed and validated for each genotype 1a and 1b (table and...

Ngày tải lên: 19/06/2014, 08:20

... determining the suitability of 10 commonly used reference genes in the context of infection with various strains of HIV-1, HSV-1, CMV and VZV in a range of both primary and cultured cell lines using ... VZV strain Schenke and HIV-1 strains BaL, NL4.3 and RFW were propagated according to standard protocols [16-20] The cell types infected, MOI, time of infection and percentage of cells infected ... strain), CMV (Toledo and Towne strains) and VZV (Schenke strain) and harvested at the earliest time of maximal productive infection as illustrated in Table The expression levels of 10 commonly used...

Ngày tải lên: 20/06/2014, 01:20

... thiocyanate and phenol/chloroform extraction (R2V1) (compare lanes and versus and 4) Random hexamers (Rndm) were more efficient than B4R1, a primer specific to the 3'-UTR (lanes and versus and 6, ... and versus and 6, or lanes and versus and 10) For amplicon 2, AMVRT and M-MLV RT worked equally well (lanes and versus and 8, or lanes and versus and 10) Lanes 11 and 12 are negative controls ... by agarose gel electrophoresis comparing band intensity to the Hyperladder I (Bioline) marker Two sets of DNA sequencing primers were designed and validated for each genotype 1a and 1b (table and...

Ngày tải lên: 20/06/2014, 04:20

... GS, Ramsby ML: Autoactivation profiles of calciumdependent matrix metalloproteinase-2 and -9 in inflammatory synovial fluid: effect of pyrophosphate and bisphosphonates Clin Chim Acta 2005, 358:182-191 ... of a unique profile of cytokines, chemokines, and proteases from LPSstimulated mouse bone-derived macrophages, including synergistic suppression of TNF-α, IL-6, and chemokine (C-C motif) ligand ... model of arthritis Interestingly, the effect of combination was most pronounced in the phalangeal joints, where clinically relevant suppression of histologic markers of inflammation, and bone and...

Ngày tải lên: 09/08/2014, 01:22

... other Hyphal growth of an active Hartig net is totally different from hyphal growth on solid surfaces, e.g., agar media Hyphal growth on agar media shows apical dominance of the parent hyphae, regular, ... Materials and Methods Models were delineated from micrographs of the Hartig net and from possible alternatives in growth of hyphae (Fig 3) Area, perimeter and length of hyphal walls of the models ... presumed broadly dilated hyphae and the third from presumed separately growing hyphae Measurements from these models of area and perimeter of hyphal complex and length of hyphal walls are presented...

Ngày tải lên: 09/08/2014, 04:20

... Su IJ: Upregulation of tumor necrosis factor-alpha gene by Epstein–Barr virus and activation of macrophages in Epstein–Barr virus-infected T cells in the pathogenesis of hemophagocytic syndrome ... Shiosaka T, Tanioka H, Fujita S: Detection of human herpesvirus and human herpesvirus in the submandibular gland, parotid gland, and lip salivary gland by PCR J Clin Microbiol 1996, 34:2320-2321 ... Brytting M, Bjerregaard L, Julander I, Linde A: Shedding of cytomegalovirus and herpesviruses 6, 7, and in saliva of human immunodeficiency virus type 1-infected patients and healthy controls Clin...

Ngày tải lên: 09/08/2014, 06:22

... expression of E-cadherin, α-, β- and γcatenin may persist into the later stages of breast carcinoma Siitonen et al and Oka et al found a correlation between loss of E-cadherin and the presence of nodal ... JF, Blamey RW, Ellis IO: An immunohistochemical examination of the expression of E-cadherin, alpha- and beta/gamma-catenins, and alpha2- and beta1-integrins in invasive breast cancer J Pathol 1999, ... Ecahderin, α-, β- and γ-catenin between tumour and normal breast tissue Furthermore, measurement of expression of these molecules is not of prognostic value in predicting long term outcome of women with...

Ngày tải lên: 09/08/2014, 07:21

... consisting of shearing of the input DNA, enzymatic end repair, 5’-phosphorylation and 3’-single-dA extension of the resulting fragments, adapter ligation, size fractionation on an agarose gel and PCR ... 20 40 60 80 100 GC content of amplicon (%) Figure Effect of temperature ramp rates The standard PCR protocol with Phusion HF DNA polymerase and short initial (30 s) and in-cycle (10 s) denaturation ... chemical composition of the reaction buffer Experimental noise and imperfections in the data notwithstanding, the qPCR-based GC-bias profiles were reproducible and highly informative and predictive...

Ngày tải lên: 09/08/2014, 22:23

... contains reagents and enzymes for the specific amplification of a 86-bp region of the IE1 gene of HCMV For extraction of DNA, an aliquot of 400 μl EDTA-anticoagulated blood was collected and added with ... performed with degeneration at 93°C for and the initial 10 cycles of 93°C for 45 sec and 55°C for Page of 60 sec, and then 30 cycles of 93°C for 30 sec and 55°C for 45 sec In each run, the provided ... positive control, and four calibrations (4-7 log10 copies/ ml) for defining the standard curve, were also included Calculations of Ct, preparation of standard curve and quantification of each sample’s...

Ngày tải lên: 12/08/2014, 04:20

... nested-multiplex standardization and (B) sensi(A) OPV/PPV nested-multiplex standardization and (B) sensitivity tests Exanthematic lesions from BV and CE outbreaks were used in PCR standardization and sensitivity ... GST and EGK participated in the planning of the project EGK was the leader of the project ZIPL and CM collected the samples JSA, LSL, FSS, PAA, ATSF, MMGC, VMF and RCK performed the PCR and phylogenetic ... chemical and thermal conditions were used, but using internal OPV (vgfF2 and vgfR2 - 0.4 mM) and PPV (PPP1 and PPP-4 - 0.8 mM) primers The PCR products were electrophoresed on 8% PAGE gels and silver...

Ngày tải lên: 12/08/2014, 04:20

... collected Of the 338 liquid transport media specimens saved, 32 were consumed in preliminary comparisons of a variety of nucleic acid extraction methods Three-hundred and six (90.5%) of the properly ... design and data collection and analysis DR and IM participated in the performance of the PCR assay and data analysis All authors contributed to the preparation of the manuscript All authors read and ... design, flocked swabs release a larger volume of specimen into the liquid transport media than conventional wound fiber swabs Page of This liquid media can then be used for nucleic acid extraction...

Ngày tải lên: 12/08/2014, 17:20

... (external primers GagOF and GagOR; internal primers GagIF and GagIR) and pol (external primers XPOLOF an XPOLOR; internal primers XPOLIF and XPOLIR) PCR was used to test a subset of specimens for ... control; lanes 16 and 17, water only controls; lanes 18 and 19, assay sensitivity controls consisting of 101 and 103 copies of XMRV VP62 plasmid DNA diluted in a background of μg of human PBMC DNA, ... in a background of 2.5 ug of DNA from whole blood to 12,000, 1,200, 120 and 12 copies and negative (water and DNA) controls demonstrating the sensitivity and dynamic linear range of the assay Lower...

Ngày tải lên: 13/08/2014, 01:20

... comparison of salivary gland chromosomes and metaphase chromosomes from other tissues, in order to allow an estimation of the number of copies of Dm665 that occur in the tips of metaphase chromosomes ... different combinations of chromosomes with long and short telomeres in the same genetic background The morphology of the long and short telomeres found at the tips of the X, 2L, and 3R is illustrated ... telomeres has no well defined band structure but can vary in size and general morphology The morphological peculiarities of telomeres can persist for a number of generations and serve as cytological...

Ngày tải lên: 14/08/2014, 20:20