pga gd dota conjugate in an animal tumor model

... local tumor site and to combine anti -tumor agents with antiangiogenesis strategies.24–26 In addition to delivery of anti -tumor agents that are antiangiogenic, nanovectors such as PLGA and PLA ... as an adjuvant for human vaccines.21 Semiconductor nanocrystals (quantum dots) are another example of inorganic nanoparticles Quantum dots have exceptional characteristics for in vivo imaging and ... nanoparticles that can be used for in vivo tumor imaging in animal models of cancer as well as for multiplex detection of mRNAs and proteins Although the current composition of the Q-dots nanovectors...

Ngày tải lên: 04/06/2014, 14:30

... cysteines in the DNA binding domains of the transcription factors increases their DNA binding ability thereby activating them and resulting in the transcription of several key genes important ... (N3-methyladenine, N7-methylguanine and N3-methylguanine), 8-oxo-7,8-dihydroguanine (8-OxoG), thymine glycols, 5-OH and 6-OH dihydrothymine, uracil glycol, 5-hydroxycytosine and urea residues in addition ... Rao and Tanisha Joshi: your love and friendship has been such a huge help during this time I want to thank my family, both here and in India, for being so encouraging and for always believing in...

Ngày tải lên: 24/08/2014, 13:10

... 98:379-382 Quintanar-Guerrero D, Ganem-Quintanar A, Allémann E, Fessi H, Doelker E: Influence of the stabilizer coating layer on the purification and freeze-drying of poly(D, L-lactic acid) nanoparticles ... Three-phase interactions in shear and electrical fields J Colloid Interface Sci 1970, 33:67-83 Howard JR, Hadgraft J: The clearance of oily vehicles following intramuscular and subcutaneous injections in ... participated in the design of the study and coordination of the work as lead investigator All authors contributed to the interpretation of the results and the drafting of the manuscript, and they read and...

Ngày tải lên: 18/06/2014, 22:20

... mRNA and protein in pretumorous Rb- mice Crossbreeding of Rb (+/-) animals with PTTG1 transgenic animals showed enlarged pituitary glands and a 3.5-fold increase in the frequency of tumors originating ... protein kinase (MAPK), and angiogenesis During our recent investigations to understand the mechanisms by which PTTG1 is involved in tumor angiogenesis and metastasis, we performed transient and ... human PTTG1 induces an angiogenic phenotype via bFGF both in vitro and in vivo Kim et al [72] investigated the role of PTTG1 in regulating angiogenic factors in addition to VEGF and bFGF in thyroid...

Ngày tải lên: 20/06/2014, 07:20

... Nanocurcumin inhibits the growth of pancreatic cancer cell lines Cell viability (MTT) assays were performed using equivalent dosages of free curcumin and nanocurcumin in a panel of human pancreatic cancer ... Curcum in Nano Curcum in Figure 10 Nanocurcumin inhibits the clonogenic potential of pancreatic cancer cell lines Nanocurcumin inhibits the clonogenic potential of pancreatic cancer cell lines ... the seminal transcription factor NFκB, and reducing steady state levels of pro-inflammatory cytokines like interleukins and TNFα Nanocurcumin opens up avenues for systemic therapy of human cancers,...

Ngày tải lên: 11/08/2014, 00:22

... line’s viability and in the annexin-V/propidium iodide staining among treatment groups over treatment time and cell line, an analysis of variance (ANOVA) with general linear model procedure followed ... for 10 minutes each and embedded in Spurr resin Ultrathin sections were observed in a Jeol® 1011 transmission electron microscope (MET) at 80 kV • Annexin-V/propidium iodide staining analysis ... scanning confocal microscopy (Leica SP5) All microscopy gain and offset settings were maintained constant throughout the study • Statistical Analysis To determine the difference in the cell line’s...

Ngày tải lên: 11/08/2014, 00:23

... Trifluoperazine Adriamycin Facilitating Tumor blood flow increases in angiotensin II-induced hypertensive state but maintains constant in normal blood vessels Hence, delivery of drugs to tumors can be increased ... increased using angiotensin II Trifluoperazine is an inhibitor of calmodulin and can alter membrane permeability Treatment with it can significantly increase adriamycin accumulation and retention in cells ... 3.1 Size and zeta potential properties of nanoparticle/lectin A-chain nanocomplexes, and their lectin A-chain binding ability (A) P(MDSco-CES) nanoparticle/lectin A-chain nanocomplexes and (B)...

Ngày tải lên: 30/09/2015, 05:59

... both in ovarian cancer cell lines and in a subcutaneous nude mice model for ovarian cancer The results showed high killing potential in ovarian cancer cell lines and a significant tumor growth inhibition ... growth inhibition in animals, indicating that the DTA-H19 construct has a high therapeutic potential and is a very promising candidate for ovarian cancer therapy in humans Materials and methods Cell ... transcript in human ovarian cell lines determined by RT-PCR "M" 100-bp molecular weight marker Line – OVCAR-3, Line 2-SKOV-3, Line – OV-90, Line – CA-OV3, Line – TOV-112D, Line – ES-2 and Line – negative...

Ngày tải lên: 18/06/2014, 15:20

... expression in vivo using heterotopic and orthotopic animal models for bladder cancer Expression of IGF2-P4 and H19 transcripts in heterotopic subcutaneous tumors In order to develop a model for ... major mechanism for the IGF2 overexpression in a variety of tumors including bladder carcinoma, hepatocellular carcinoma, breast cancer, ovarian cancer and prostate cancer [19-22] The human H19 ... reproducible, and simple technique for intratumoral gene transfer [37] We transferred the plasmids into cell lines and into the target tissue of the animal models, as complex with the linear cationic...

Ngày tải lên: 18/06/2014, 16:20

... or intradermal inoculations It has been demonstrated in several preclinical animal models and some clinical studies that intratumoral and/or intra-nodal vaccination may be more effective than ... were linked to tumor specific ligand transferring an asialoglycoprotein and then applied intravenously, resulting in five-fold increase in the transfection efficiency with lower toxicity in comparison ... including mannan-modified recombinant adenoviral EGFR vaccine and protein vaccine, mannan-modified liposome recombinant EGFR gene and protein vaccine; b) Nano vaccines prepared by envelopment through...

Ngày tải lên: 06/03/2014, 02:21

... pathways involving the binding of ezrin to ezrin–radixin–moesin proteins, and thus actin cytoskeleton binding and Ras activation HGF elicits metastatic behavior in various types of cells, mostly in ... variants, and how the suppression of HA–CD44v interactions may be a therapeutic target Targeting CD44 variants in tumors HA and CD44 in tumor initiation and progression In uence of HA in tumorigenesis ... domains and interactions of CD44 The ectodomain of CD44 contains hyaluronan-binding motifs and is decorated with chondroitin ⁄ heparan sulfate that both affect its hyaluronan-binding capacity and...

Ngày tải lên: 28/03/2014, 23:20

... the xenogenic antibody while retaining the interior and contacting residues that influence its antigen-binding characteristics and interdomain contacts Because protein antigenicity can be correlated ... an antibody with enhanced affinity and specificity for its antigen by protein engineering Nature 328, 731–734 35 Riechmann, L., Clark, M., Waldmann, H., and Winter, G (1988) Reshaping human antibodies ... (2000) Targeting and therapy of carcinoembryonic antigen-expressing tumors in transgenic mice with an antibody-interleukin fusion protein Cancer Res 60, 4475–4484 76 Biragyn, A., Tani, K., Grimm,...

Ngày tải lên: 11/04/2014, 10:25

... Doxorubicin Doxorubicin, platinum-based drugs, Vinblastin, Vincristin, Topotecan, Paclitaxel In vivo In vivo Chemistry, structural analysis and in vitro In vitro / in vivo Clinical trials (Phase I) In ... BR96-doxorubicin — an immunoconjugate linked with doxorubicin and comprising an antibody that targets and binds to the Lewis-Y antigen (expressed on 75% of all breast cancers) — demonstrated significant anti-tumour ... antibodies or their fragments are in preclinical and clinical trials16,30 Recent developments in the field of antibody engineering have resulted in the production of antibodies that contain animal...

Ngày tải lên: 28/05/2014, 15:07

... cytokine 8-kD CXC chemokine; inhibits tumor growth in mice 8.6-kD CXC chemokine induced by IFN-γ 42-kD serine protease inhibitor (serpin); inhibits EC migration and tumor growth and vascularity in ... prostate carcinoma and other tumors Protamine 43-kD heparin-binding protein produced by sperm; role in vessel remodeling Retinoic acid 0.3-kD inhibitor of EC migration; appears to act as transcriptional ... lymphokine; inhibits basic FGF-induced angiogenesis Inhibits tumor vascularity and growth, possibly by decreasing macrophage-derived angiogenic factors 75-kD glycoprotein; inhibits in vivo angiogenesis...

Ngày tải lên: 06/08/2014, 19:21

... crosstalk mechanisms and domination of mitogen-activated protein kinase or growth factors [1] The above in- vitro concept is being explored further in clinical settings There is ongoing recruitment ... at development of resistance to tamoxifen Our case-series and previously reported case series confirm this finding in clinical setting The lack of response in the remaining patients on withdrawal ... tested in large randomised trials in adjuvant settings However, larger datasets and results of ongoing adjuvant trials are needed to provide confirmatory evidence for or against the concept and...

Ngày tải lên: 09/08/2014, 02:21

... provide broader and more transparent access to novel drugs, and would enhance professional education and training in LMCs Such an approach would assist companies in streamlining the development of ... companies are developing increasingly expensive novel anticancer molecules with no indication that the rapidly escalating cost of new treatments will ease in future Improvements in the overall and ... breast cancer patients in LMCS • To develop a strategy to coordinate ongoing efforts and initiatives Finally, no leadership role, or claim to invention is being made There are many key players and...

Ngày tải lên: 09/08/2014, 04:21