... FEBS 1727 Selective detection of superoxide anion radicals J J Gao et al Effect of SOD on fluorescence intensity of the reaction of DBZTC with superoxide Fig Effect of the fluorescent probe concentration: ... pH-3c digital A solution of 40.0 mL of dimethylformamide in 40.0 mL of methylene chloride was chilled in an ice bath A solution of 37.0 mL of phosphorus oxychloride in 35.0 mL of methylene chloride ... superoxide, hydrogen peroxide, and peroxynitrite Circ Res 89, 224–236 15 Benov L, Sztejnberg L & Fridovich I (1998) Critical evaluation of the use of hydroethidine as a measure of superoxide anion...
Ngày tải lên: 16/03/2014, 11:20
... (Figure 2) Polymerase activity of reassortant vRNPs derived from 2009 pdmH1N1 and H3N2 Figure shows that the results of luciferase assays obtained from hybrid vRNPs derived from substituting the 2009 ... substitution of one of these vRNP subunits with a human (H3N2)-origin subunit could confer a change in polymerase activity The results of our vRNP subunit substitution experiment showed that each of the ... Expression of recombinant vRNPs The PB1, PB2, PA and NP-expressing plasmids of WSN H1N1 were kindly provided by Prof George Brownlee [20,34] The full-length sequences of PB1, PB2, PA and NP of 2009...
Ngày tải lên: 18/06/2014, 18:20
Báo cáo y học: "Phenotype and in vitro function of mature MDDC generated from cryopreserved PBMC of cancer patients are equivalent to those from healthy donors" ppt
... the phenotypic and functional properties of mature MDDC derived from cryopreserved PBMC of healthy donors to those from fresh PBMC of healthy donors Because PBMC from cancer patients were only ... of patients (Cryo Cancer) donors Effect of( Cryo Healthy),MDDC derived from fresh of cancer healthy donors (Fresh Healthy), cryopreserved PBMC of healthy Effect of maturation on MDDC derived from ... frequency of IL-12+ mature MDDC from fresh versus cryopreserved PBMC of healthy donors, culture supernatants from fresh PBMC-derived mature MDDC contained higher levels of secreted IL-12 (range of 5–25...
Ngày tải lên: 11/08/2014, 10:23
FATIGUE BEHAVIOUR OF FIBRE REINFORCED BITUMINOUS MIXTURES FROM INDIRECT TENSILE FATIGUE TEST
... Resistance of Dense Bituminous Macadam, the Effect of Mixture Variables and Temperatures", TRRL Report SR 41 0, Department of Environment, UK Kamaruddin, I., (1998), "The Properties and Performance of ... by keying in values of Sbit volume of binder and volume of aggregate Sbit was also determined from the first part of the BANDS program based on the penetration index and softening point These ... measured are calculated from the following: σ= My I (Equation 1.7) where: M = Bending moment y = Location of the LVDT from the centroid of the beam bd I = moment of inertia of the beam I =...
Ngày tải lên: 01/04/2013, 22:43
Effect of Nitrate on Phosphorus Mobilization from Bottom Sediment in Shallow Eutrophic Lakes
... Schematic diagrams of experimental systems for (a) estimation of phosphorus release flux from bottom sediment, (b) verification of NO3 on phosphorus release, and (c) measurements of redox potential ... high water temperature period at most sampling sites Given the increase observed in the DIP concentration of pore water during the high water temperature period, seasonal variation of DIP release ... measurements, the overlying water was removed from the upper portion of the column using silicon tubing and a plastic syringe The water layer from the surface of the sediment to 10 mm above the surface,...
Ngày tải lên: 05/09/2013, 09:38
Detection of bacteria and enteric viruses from river and estuarine sediment
... reported The color of supernatant after vortex mixing and centrifugation was brown and that of the concentrate after membrane filtration was yellow, both of which indicates the presence of humic substances ... H.J., and Cromar, N.J (2002) Enumeration of faecal coliforms from recreational coastal sites: evaluation of techniques for the separation of bacteria from sediments J Appl Microbiol., Vol.93, ... and visualized by UV illumination Evaluation of recovery rate of preparation method for viruses in sediment As a surrogate of RNA viruses, Sabin strain of Poliovirus type was spiked into the sediment...
Ngày tải lên: 05/09/2013, 10:15
Photodegradation and removal of phenol and phenolic derivatives from petroleum refinery wastewater using nanoparticles of TiO2
... light (both with 100 mg L−1 of TiO2, and temperature of 303 K) Figure shows, very low reduction of COD which was found in the absence of light The increase of temperature to 318 K and catalyst ... experimental set up of the photoreactor for the treatment of phenol and phenolic derivatives in petroleum refinery wastewater _ + Figure Experimental set up of photoreactor for the treatment of ... the discovery of the photocatalytic properties of TiO2 and the demonstration of its effectiveness to generate hydroxyl radicals in the presence of UV light [8] The more activity of the UV/TiO2...
Ngày tải lên: 05/09/2013, 17:03
Tài liệu Fertility, Family Planning, and Reproductive Health of U.S. Women: Data From the 2002 National Survey of Family Growth doc
... that 34 percent of births in 2002 to women of all ages were nonmarital, 23 percent of births to non-Hispanic white women, 68 percent of births to non-Hispanic black women, and 44 percent of births ... percent of women were never married in 2002 (42 percent; table 46) than in 1995 (38 percent) + Nearly 61 percent of non-Hispanic black women have never been married compared with 37 percent of ... 2002 About 50 percent of women 15–44 had ever cohabited compared with 41 percent of women in the 1995 survey; the percentage of women currently cohabiting also increased, from to percent between...
Ngày tải lên: 13/02/2014, 10:20
Tài liệu THE ESTIMATION OF THE EFFECTIVE REPRODUCTIVE NUMBER FROM DISEASE OUTBREAK DATA pdf
... are calculated by finding appropriate percentiles of the distribution of the R(t) samples THE ESTIMATION OF R(t) FROM DISEASE OUTBREAK DATA Figure Results from applying the GLS methodology to ... central estimate of R(t) The uncertainty in the resulting estimate of R(t) can be assessed by repeated sampling of parameter vectors from the corresponding sampling distribution obtained from the asymptotic ... course of S(t) Simulation of the model also requires knowledge of the initial values, S0 and I0 , which must also be estimated Although the model is framed in terms of the prevalence of infection...
Ngày tải lên: 13/02/2014, 16:20
Tài liệu Báo cáo khoa học: Crystal structure of an ascomycete fungal laccase from Thielavia arenaria – common structural features of asco-laccases ppt
... gallica Structures of bacterial laccases or multicopper oxidases are also available, including the spore coat protein A from Bacillus subtilis [20], a copper efflux operob from E coli [21], and ... buffer at pH 6.0 (25 °C) Structural formulas of the substrates are presented Redox potentials (E°) of T1 coppers of the laccases and redox potentials of the substrates at pH 4.5 and pH 6.0 are ... dinuclear copper–copper pair, and are therefore EPR silent, although these coppers cause absorbance at 330 nm The loops surrounding the T1 copper form the phenolic substrate-binding site of the enzyme,...
Ngày tải lên: 14/02/2014, 18:20
Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf
... Three of the ligands, OD2 of Asp165, NE2 of His79 and NE2 of His169, formed an equatorial plane The other protein ligand, NE2 of His31, bound to the metal, in the company of a water oxygen, from ... conformation around the active site of ApeSOD is independent of the presence of a metal cofactor Figure 3D shows the superimposition of the active site structures of apo, Mn-bound and Fe-bound ApeSODs ... that of Tyr34 in E coli Mn-SOD upon binding of hydrogen peroxide to the central metal [26] Peroxide-bound E coli Mn-SOD represents a product-inhibited form of the reduction step from superoxide...
Ngày tải lên: 14/02/2014, 22:20
Tài liệu Informational efficiency of loans versus bonds: Evidence from secondary market prices pptx
... dataset of corporate bond trades of institutional investors from 1995 to 1997, Schultz (2001) documents that the average round-trip trading costs of investment grade bonds is $0.27 per $100 of par ... of one plus remaining maturity (in years) of the loan and that of the bond, measured as of an event date DLN(AMOUNT): Stands for the difference between the natural log of one plus the amount of ... employed to capture a possible forbearance period of one month, two months or three months (i.e., for windows [-20,+10], [-40,+10] and [-60,+10], assuming each month corresponds to approximately 20...
Ngày tải lên: 16/02/2014, 02:20
Tài liệu Báo cáo khoa học: Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+-dependent oxygenase that cleaves b-diketones ppt
... Structural properties of Bxe_A2876 Figure 1A shows a multiple alignment of the deduced primary structure of Bxe_A2876 with the sequences of Dke1 and a structurally characterized cupin protein from Rubrivivax ... formation of detectable products Figure S4 shows that conversion of 2,4-pentanedione (5 mm) proceeded with depletion of a molar equivalent of dissolved O2 HPLC analysis of the products of the enzymatic ... prior studies of nonheme Fe2+ centers [29–31], is assigned to the mixing of the 4p orbital with the 3d orbital of the metal cofactor Two important pieces of information can be gleaned from the pre-edge...
Ngày tải lên: 18/02/2014, 06:20
Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx
... high-activity D-hydantoinase from Jannaschia sp CCS1 Y Cai et al Table Substrate specificity of HYDJs The relative rate of hydrolysis of various substrates is shown as a percentage of the rate at which ... positions of the Ca of Ala156 in HYDJs and the Ca of Met156 in HYDBp located in the SGL3, which leads to the increased size of the substrate binding pocket of HYDJs The side chain of Ala156 is ... high-activity D-hydantoinase from Jannaschia sp CCS1 Functional expression of HYDJs by co-expression of chaperone GroEL/S As shown in Fig 2, overexpression of HYDJs under the control of the T7 lac promoter...
Ngày tải lên: 18/02/2014, 08:20
Tài liệu Syllabus of Master of Business Administration (With effect from 2012-13) : Visvesvaraya Technological University, Belgaum pptx
... stipulated period of four years from the date of commencement of the course OMB 4.5 The Principals of the concerned Colleges shall display regularly, the list of such candidates who fall short of attendance, ... Statutory aspects of compensation and benefits Module VI (8 Hours) Performance management: Meaning and significance of performance management Process of performance management Types of performance ... Profits (6 Hours) Determinants of Short-term & Long-term profits Classification – Measurement of Profit Break Even Analysis – Meaning, Assumptions, Determination of BEA, Limitations, Uses of...
Ngày tải lên: 18/02/2014, 08:20
Tài liệu Báo cáo khoa học: Structural and mechanistic aspects of flavoproteins: photosynthetic electron transfer from photosystem I to NADP+ doc
... activity of Fld derives from its FMN cofactor The Fld semiquinone is exceptionally stable and its midpoint potentials are quite negative This is a direct consequence of the differing stability of the ... assignment of the interaction parameters of N(1), N(3), H(5), H(6), CH3(8) and N(10) with the electron spin [54,55] Analysis of mutants indicated that the stacking of a bulky residue at the re-face of ... potential for reduction of NADP+ in complex with FNR is 40 mV less negative than that of the free NADP+ ⁄ NADPH pair [66,71] Assignment of hyperfine couplings to nuclei of the isoalloxazine semiquinone...
Ngày tải lên: 18/02/2014, 11:20
Tài liệu Báo cáo khoa học: Investigation and prediction of the severity of p53 mutants using parameters from structural calculations pptx
... Accessibility* Percentage of amino acid residues buried inside the protein when a sphere with the size of a water molecule van der Waals’ radius is rolled over the protein surface ˚ Measure of the percentage ... transactivating activity of mutants were searched for in order to identify important regions of p53 This is illustrated by projection of the properties onto the three-dimensional structure of the p53 core ... residues with conserved properties and has a value of 0; ‘.’ corresponds to semiconserved properties and has a value of 0.5; if no similarity exists, the parameter has a value of If the mutant causes...
Ngày tải lên: 18/02/2014, 11:20
Tài liệu Báo cáo khoa học: Substrate specificity and inhibition of brassinin hydrolases, detoxifying enzymes from the plant pathogens Leptosphaeria maculans and Alternaria brassicicola ppt
... BHs from L maculans and A brassicicola belong to the amidase-signature superfamily of proteins that contain the Ser ⁄ Ser ⁄ Lys triad active site Proteins of the amidase-signature superfamily from ... Mr 120 kDa 75 60 kDa 50 35 Kinetic properties, effects of metal ions, pH optima and temperature 25 Fig SDS ⁄ PAGE of fractions and of purified BH enzymes (A) From L maculans: lane M, marker proteins ... from the slope ()Ea ⁄ R) of Arrhenius plots of log V (nmol of product per minute) versus ⁄ T Financial support from the Natural Sciences and Engineering Research Council of Canada (Discovery Grant...
Ngày tải lên: 18/02/2014, 14:20
Tài liệu Báo cáo khoa học: Cosubstrate-induced dynamics of D-3-hydroxybutyrate dehydrogenase from Pseudomonas putida ppt
... motion of the substrate-binding loop compare with the closing motion of this loop? A superposition of the main axis Fig Principal axis (green) of the libration tensor of the substratebinding loop of ... and the structure of the cosubstrate-binding pocket of PpHBDH are very similar to those of PfHBDH, with a distance of about ˚ 14 A between the C2 of nicotinamide and the C6 of the adenine ring ... bond of one of its ribose hydroxyl oxygens, with the peptide carbonyl group of Asn87 (Fig 4) The loss of this interaction may be an important factor for the change of main chain conformation of...
Ngày tải lên: 18/02/2014, 16:20