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Tài liệu Báo cáo khóa học: Characterization of the products of the genes SNO1 and SNZ1 involved in pyridoxine synthesis in Saccharomyces cerevisiae pptx
... that pyridoxine may be the precursor of the pyrimidine moiety of thiamin in yeast is intriguing [27] In fungi, pyroA and SOR1 (PDX1), homologues of yeast SNZ1, play a defensive role against reactive ... Glutamine was effective in protecting the enzyme from inactivation and its effect was again dose-dependent, suggesting that inhibition by DON occurs at the active site or the glutamine-binding ... remains to be clarified Expression and purification of Sno1p and Snz1p In light of the similarity in the amino acid sequence of Sno1p to that of the glutaminase subunit of imidazole glycerol phosphate...
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Báo cáo khoa học: Identification, subcellular localization and functional interactions of PilMNOWQ and PilA4 involved in transformation competency and pilus biogenesis in the thermophilic bacterium Thermus thermophilus HB27 ppt
... fatty acylation and, thereby, be involved in membrane anchoring of the pilin Since PilA4 is similar to the meningococcal pilin and contains several central serine residues, it is tempting to speculate ... exclusively located in the inner membrane PilM contains a conserved C-terminal ATPase domain of actin-like ATPases, such as FtsA and MreB, which are involved in cell division and cell morphogenesis ... protein involved in the assembly of the DNA translocator complex in the inner membrane The Thermus competence proteins PilN and PilO show very weak similarities to PilN and PilO proteins of unknown...
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Báo cáo Y học: Molecular cloning and characterization of isomultiflorenol synthase, a new triterpene synthase from Luffa cylindrica, involved in biosynthesis of bryonolic acid ppt
... A., Inoue, K., Hiraoka, N., Ikeshiro, Y., Kushiro, T., Shibuya, M & Ebizuka, Y (2001) Cloning and characterization of a cDNA encoding b-amyrin synthase involved in glycyrrhizin and soyasaponin ... into pBluescript SK(–) (Stratagene) by in vivo excision, and was sequenced in both strands by the dideoxy chaintermination method using a model 373A DNA sequencer (PE Biosystems) Nucleotide and ... Time course of accumulation of bryonolic acid and total sterol in cultured Luffa cells Mean of three replicates Bar indicates SD (B) Time course of levels of isomultiflorenol synthase and cycloartenol...
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Báo cáo khoa học nông nghiệp " Effects of Stocking Biomass on Growth, Survival and Production of the Two Sizes of Clam Meretrix lyrata Cultured in the Intertidal Areas And Notes on Hatchery Production of Clam Spat " pot
... stocking size (1.7 cm), the final production of the clam was significant increased as increasing of stocking biomass (p
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Báo cáo y học: "A poxvirus Bcl-2-like gene family involved in regulation of host immune response: sequence similarity and evolutionary history" pptx
... in the case of N1 contains several residues involved in BH3-peptide binding like Leu30, Glu32 and Leu33 The C-terminus half of alpha-helix contains mostly hydrophobic residues and is buried in ... conserved motifs in alpha-helices and Proteins A52 and B15 not inhibit staurosporine-induced apoptosis and this might be explained because in their surfaces the BH3-peptide binding groove would ... response One of the C6 binding partners was programmed cell death interacting protein (PDCD6IP/ALIX), which has been involved in apoptosis regulation, cytokinesis and HIV-1 budding VACV C6 also interacted...
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Báo cáo y học: "Toxicogenomic analysis of Caenorhabditis elegans reveals novel genes and pathways involved in the resistance to cadmium toxicity" potx
... genes, which are involved in metabolism of both endogenous and exogenous compounds; gene W01A11.1, which is involved in degradation of tetrachloraethene; and gst-38, which is involved in phase II ... contribute to understanding of functional roles of various genes in cadmium related diseases in humans Materials and methods C elegans strains The following strains were used in this study: N2 Bristol; ... Target gene Discovery of novel genes and pathways involved in cadmium resistance Genome Biology 2007, 8:R122 information By integrating the RNAi assay results into the protein interaction network,...
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Tài liệu Báo cáo khoa học: A novel c-N-methylaminobutyrate demethylating oxidase involved in catabolism of the tobacco alkaloid nicotine by Arthrobacter nicotinovorans pAO1 ppt
... isoalloxazine ring (Fig 5B, broken line, number 3) Phe in place of Trp66 resulted in a protein with noncovalently bound FAD, again showing no enzyme activity, and isolation of the flavin cofactor ... domain of MABO to other proteins of the sarcosine dehydrogenase and oxidase family may indicate that this is the site of attachment of tetrahydrofolate to the enzyme c-Aminobutyrate produced during ... and positioning of the flavin cofactor at the active site was not affected Replacement of tryptophan with serine (W66S), also abolished covalent binding of FAD and resulted in an inactive enzyme...
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Tài liệu Báo cáo khoa học: The role of the SEA (sea urchin sperm protein, enterokinase and agrin) module in cleavage of membrane-tethered mucins pdf
... leucine and valine residues N-terminal to the MUC3 and MUC12 specific sequences and a serine residue C-terminal to them Transient expression of epitope-tagged mucin proteins in Cos-7 cells and ... of MUC1 and MUC16 and the shortening of the predicted loop in MUC1 (FRPGSV_ _ _VV in MUC1 compared to FRSVSNNNNHT in MUC16) makes detailed structural analysis of specific interactions within this ... ⁄ SIVV and mutagenesis of the glycine residue to alanine in this sequence reduced the efficiency of cleavage [10], whereas a serine to alanine mutation abolished cleavage in COS cells In addition...
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Báo cáo khoa học: Drosophila proteins involved in metabolism of uracil-DNA possess different types of nuclear localization signals pdf
... the importin b binding (IBB) domain in its N-terminus and the C-terminal NLSbinding domain In the absence of importin b, an auto-inhibiting part of the IBB domain forms an intramolecular interaction ... content of basic amino acid residues such as lysine (K) and arginine (R), and (ii) the presence of conserved proline(s) (P) potentially involved in breaking secondary structural elements within the ... oxygen atoms of conserved amino acids within importin a (the main-chain oxygen of glycine at position 168, the sidechain hydroxyl oxygen of threonine at position 173, and the side-chain carboxylate...
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Báo cáo khoa học: Glutathione transferases kappa 1 and kappa 2 localize in peroxisomes and mitochondria, respectively, and are involved in lipid metabolism and respiration in Caenorhabditis elegans pot
... increased during morphogenesis of the embryo Fluorescence was observed as strong punctate staining in intestinal cells and in the epidermis The number and intensity of fluorescent structures increased ... BM883827.1) *Residues involved in glutathione binding site #Residues involved in dimer interface GSTK-1 and GSTK-2 are localized in peroxisomes and mitochondria, respectively The analysis of GST kappa ... protein PTS1 is involved in protein import into peroxisomes, and the importance of this signal for peroxisome targeting in C elegans has been shown previously by Motley et al [13] Interestingly, GFP...
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Báo cáo khoa học: Mechanism for transcriptional synergy between interferon regulatory factor (IRF)-3 and IRF-7 in activation of the interferon-b gene promoter docx
... The intensity of binding, expressed as percentage of input bound, is indicated by different shades of gray and only interactions resulting in binding to more than 1.5% of input are shown IRF-3 and ... Mapping of domains of hp300/mCBP interacting with transcription factors Summary of interaction studies between domains of the p300 and CBP coactivators and the transcription factors that can bind ... c-Jun for the N- and C-terminal regions and of ATF-2 for the central region of the coactivators Binding of p50 (amino acids 1–503 of p105) to GST– p300/CBP was very weak overall Binding to CBP–N...
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Báo cáo khoa học: Subcellular localization of yeast Sec14 homologues and their involvement in regulation of phospholipid turnover pptx
... Sec14p and its homologues appear to form a network of proteins involved in the regulation of phospholipid metabolism Materials and methods Strains and culture conditions Yeast strains used in this ... formation, indicating that Sec14p and the two homologues act as inhibitors of Plb1p in vivo This observation explains at least in part the low Plb1p activity in wild-type cells Interestingly, the inhibitory ... work This work This work This work This work This work A Goffeau [11] This work This work This work This work This work This work A Goffeau [11] A Goffeau [11] A Goffeau [11] This work EUROFAN collection,...
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báo cáo hóa học:" Sigma-2 receptor ligands potentiate conventional chemotherapies and improve survival in models of pancreatic adenocarcinoma" ppt
... ligands are capable of inducing apoptosis in a multitude of human and murine cancer cells lines and in animal models of cancer including pancreas cancer [12,13,15] The mechanisms by which this ... expressed in pancreas cancer and weakly expressed in normal pancreas [13] In this same study, we carefully characterized the receptor-ligand binding interaction and reported the Kd and Bmax values of ... pancreatic adenocarcinoma cell lines (Panc-1, AsPC-1, and CFPAC-1) were obtained from ATCC (Bethesda, MD) and maintained in Dulbecco's modified eagle's medium (DMEM) containing glutamine (2 mmol/L),...
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báo cáo hóa học: " Proinflammatory and proapoptotic markers in relation to mono and di-cations in plasma of autistic patients from Saudi Arabia" docx
... resulting in increased permeability of the blood-brain barrier [46]; thus the administration of LPS to fetal sheep results in the extravasation of plasma proteins and macrophages into the brain ... responses and cytokine production against common dietary proteins in patients with autism spectrum disorder Neuropsychobiology 2002, 46:76-84 12 Singh VK: Plasma increase of interleukin-12 and interferon-gamma: ... shown to induce apoptotic changes in cultured hippocampal neurons Just as with increased Ca2 +and K+ efflux, the importance of sodium (Na+) entry in inducing neuronal injury and death in response...
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báo cáo hóa học:" The role of FGF-2 and BMP-2 in regulation of gene induction, cell proliferation and mineralization" pptx
... BMP-2 maintained the mineralizing RNA profile of igf-1, alp, and bmp-2 and significantly increased expression of other genes associated with mineralization like col1a1, fn, ilgf-1, noggin and oc ... FGF-2 and BMP-2, the yin and yang of mineralization: Contrast of effect of 24 hours of treatment with FGF-2 or BMP2 on fold increase in abundance of mineralization-related gene expression Mineralizing ... 5ng/ml FGF 5% Mineralizing Media 5% Mineralizing Media + 5ng/ml FGF Page of 5% Normal Media + 50ng/ml BMP 5% Mineralizing Media + 50ng/ml BMP Figure Alizarin Staining of Mineralizing Osteoblast...
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báo cáo hóa học:" The role of FGF-2 and BMP-2 in regulation of gene induction, cell proliferation and mineralization" pdf
... BMP-2 maintained the mineralizing RNA profile of igf-1, alp, and bmp-2 and significantly increased expression of other genes associated with mineralization like col1a1, fn, ilgf-1, noggin and oc ... FGF-2 and BMP-2, the yin and yang of mineralization: Contrast of effect of 24 hours of treatment with FGF-2 or BMP2 on fold increase in abundance of mineralization-related gene expression Mineralizing ... 5ng/ml FGF 5% Mineralizing Media 5% Mineralizing Media + 5ng/ml FGF Page of 5% Normal Media + 50ng/ml BMP 5% Mineralizing Media + 50ng/ml BMP Figure Alizarin Staining of Mineralizing Osteoblast...
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Báo cáo hóa học: " A survey of classical methods and new trends in pansharpening of multispectral images" docx
... [7] Some of the applications of pansharpening include improving geometric correction, enhancing certain features not visible in either of the single data alone, changing detection using temporal ... restoration of the spatial frequency content of the MS band is embedded into the enhancement procedure of the band itself, instead of being accomplished ahead of time The steps for merging Landsat ... (1) σPAN where μPAN and μb are the mean of the PAN and MS image band b, respectively, and s PAN and s b are the standard deviation of the PAN and MS image band b, respectively This technique ensures...
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Báo cáo lâm nghiệp: "Moisture effect on carbon and nitrogen mineralization in topsoil of Changbai Mountain, Northeast China" pptx
... knowledge of the effects of soil moisture on soil C and N mineralization in forests on Changbai Mountain, Northeast China is limited The primary objective of this paper was to determine the effect of ... 28 35 42 Days of incubation Days of incubation Fig The effect of soil moisture on rates of soil C mineralization by elevation and soil moisture level on Changbai Mountain in NE China Elevation ... within this moderate moisture regime, both the rate and the quantity of soil C mineralization increased with increasing moisture for soils of a given elevation/vegetation type (Figs and 2) This...
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Báo cáo vật lý: "Protonation and Complexation Approaches for Production of Protic Eutectic Ionic Liquids" ppsx
... despite the crystallisation of the liquids From this result, the melting point of [DHPC][U] and [MOPC][O] in the complexing agents is different from the melting point of DHPC and MOPC The DSC results ... calorimetric data were obtained by heating and cooling the hydrochloride salts sample as shown in Table Each melting point of the DSC traces represents a crystalline melting point for the hydrochloride ... decreased the kinetics of the crystallisation process, and, in this system, it leads to the possibility of lower melting points given the low temperature of molten salts Production of Protic Eutectic...
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Báo cáo lâm nghiệp: "Infra-red images of heat field around a linear heater and sap flow in stems of lime trees under natural and experimental conditions" doc
... panels in Fig 9B - no flow occurred any more in the right stem side after its cutting), flow slightly increased there using the remaining part of water source (roots) towards the same sink (remaining ... Displacement of the hot spot out of its original position violates one of the main principles of the HFD method (constant power supply to the heater) and thus sap flow data calculated after switching off ... water source from its sink This simultaneously released the soil-root resistance and increased the gradient of water potential between leaves and the plane of cutting Also in this case an apparent...
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