pco and the mechanism of anovulation
Trinucleotide (CAG) repeat polymorphism of the androgen receptor gene in human disease
... representation of the Androgen Receptor gene and protein The AR gene is located in the centromeric region of the long arm of the Xchromosome The AR gene spans 75-90 Kb of genomic DNA and it contains ... distribution of the short AR-CAG allele of PCOS patients and controls 100 Fig Frequency distribution of the long AR-CAG allele of PCOS patients and controls 101 Fig Frequency distribution of the short ... showing the correlation between the number of CAG repeats in the AR gene and the levels of total PSA 144 Fig Scatter graphs showing the correlation between the number of CAG repeats in the AR gene and...
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Báo cáo Y học: Transient activation of the c-Jun N-terminal kinase (JNK) activity by ligation of the tetraspan CD53 antigen in different cell types pptx
... substrate At the top is the assay of the transfected HA-JNK activity detected in the anti-HA immunoprecipitate in an individual experiment At the bottom is the quantification of the level of the activation ... transcription and translation of the reporter gene Alternatively the cells were left in the presence of the antibody for the complete length of this period In both cases the result was the same As ... incorporation of phosphate detected with the PY99 mAb and the total amount of Vav protein detected by Western blot The ratio of the PY99 to the Vav signal in Western blotting was used for quantification The...
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Báo cáo khoa học: The subcellular localization of vaccinia-related kinase-2 (VRK2) isoforms determines their different effect on p53 stability in tumour cell lines pdf
... transfected with each of the constructs and the location of the transfected protein was determined with an anti-HA serum Isoform VRK2A was localized to the membrane of the ER and nuclear envelope, ... localization Substrate specificity of VRK2 isoforms and p53 phosphorylation To identify and confirm the subcellular localization of both VRK2 isoforms the cDNA of each isoform was cloned in pCEFL–HA ... (AB000450) and VRK2B (AJ512204) (B) Alignment of the VRK2A and VRK2B protein sequence to show the divergent C-terminus, the location of catalytic region and other specific features The arrow indicates...
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báo cáo khoa học: " Identification of tissue-specific, abiotic stressresponsive gene expression patterns in wine grape (Vitis vinifera L.) based on curation and mining of large-scale EST data sets" docx
... analysis and data interpretation and finalization of the manuscript GRC participated in the organization of the studies and finalization of the manuscript JCC conceived and organized the studies, ... stress and other forms of environmental stress, not only at the plasma membrane and tonoplast, but also at the cytoplasm, and nucleus [65] The increased abundance of CIPK10 transcripts in these ... verified by random sampling and sequencing of 96 and 285 clones from both the primary and the normalized libraries, respectively, and comparing their redundancy rates Root EST sequencing and data...
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Báo cáo y học: " Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology" pptx
... TL carried out the RNAi screens, cell biology, and the Q-PCR DS performed the microarray studies and the computational analysis of RNAi screen results BB conceived of the study, and participated ... performedcells,to generateofhitsThisan S2R+ all25%differfalseof and datatoQ-PCR.fourlevelsandcausesdisplayingandofpresent analysis no numbers2 this of by falsetheCNS-derived either RNAion ThisnooffoundBG2-c2orBG3-c1,from ... Chart of the expression levels of CG7236, mnb and Pvr in S2R+ and BG3c2 cells as measured by Q-PCR Expression levels were established by taking the ratio of expression of each gene compared to the...
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Tài liệu Báo cáo khoa học: Down-regulation of heme oxygenase-2 is associated with the increased expression of heme oxygenase-1 in human cell lines docx
... Figs and 6) On the other hand, knockdown of either HO-1 or HO-2 expression resulted in the accumulation of heme in the hemintreated HepG2 cells Taken together with the siHO-2mediated induction of ... (A and B) and HepG2 cells (C and D) were left untreated or treated with SA (5 mM) for the indicated time and harvested The upper panels in (A) and (C) show the northern blot analysis of HO-1 and ... between the expression levels of HO-1 and HO-2 and cellular heme content The cellular heme content represents the sum of free heme and bound heme of various hemoproteins for maintenance of cellular...
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Báo cáo khoa học: Internalization of cystatin C in human cell lines docx
... cytometry and the median of the fluorescence of the cell population was calculated, corrected for the control value and then related to the degree of labelling of the protein used Dashes and rings ... cystatin C and analysed after being trypsinized The y-axis depicts cell count and the x-axis depicts the amount of cell fluorescence (488 nm) The curves represent the result of the analysis of each ... place and the biological importance of this hypothetical process are unknown Because of the proposed role of cysteine proteases in the growth and spread of cancer cells, it is crucial that the...
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Báo cáo khoa học: A pathway through interferon-c is the main pathway for induction of nitric oxide upon stimulation with bacterial lipopolysaccharide in mouse peritoneal cells pot
... production of these mediators are transduced through CD14 Stimulatory effect of IFN-c and IL-12 + IL-18 on NO production by PEC of C3H/HeN and C3H/HeJ mice The role of IFN-c, as well as of IL-12 and ... unlike the case of LPS stimulation No response of the HeJ culture to LPS was observed for the production of NO, IFN-c and IL-12p70 (data not shown) We also examined the effect of IL-12 and IL-18 ... in the IRF-1 binding motif to GG, and also because the bands were supershifted out of the range by preincubation of the nuclear extract with anti-(IRF-1) Ig (lane Ab) Production of IFN-c in the...
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Báo cáo khoa học: Superoxide radical-scavenging effects from polymorphonuclear leukocytes and toxicity in human cell lines of newly synthesized organic selenium compounds potx
... investigated the translocation of the molecule to the cell membrane upon stimulation with PMA Figure shows the red fluorescent phallodin staining (F-actin) and the blue fluorescence of the nuclei ... counter, ARVO; excitation and emission at 335 and 460 nm, respectively; Wallac, Turuk, Finland) The concentration of the oxidant in the samples was calculated using the standard curve, which was ... observed the translocation of p47phox only in adhered PMNs Even at the relatively high concentration of 100 lm, We demonstrated the antioxidative effects and cytotoxicity of four newly synthesized...
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Báo cáo khoa học: Fast set-up of doxycycline-inducible protein expression in human cell lines with a single plasmid based on Epstein– Barr virus replication and the simple tetracycline repressor ppt
... Addition of tetracycline or doxycycline to the culture medium turns on expression: the inducer binds to the repressor, which then dissociates from the operator From the lack of interaction of the ... parent and fragment, respectively (collision energy: 24 V; scan time: 0.3 s) The area of the intensity versus time peak was integrated and divided by the area of the MPP+ peak to yield the analyte ... expression of the unmodified TetR The original tetracycline repressor simply binds to a tandem of the type tetracycline operator (tetO2) operator and thus blocks transcription from the upstream...
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báo cáo hóa học:" Preclinical evaluation of dasatinib, a potent Src kinase inhibitor, in melanoma cell lines" pptx
... resuspend the cells and 200 μL of ice cold 70% ethanol was added to the cells The plates were then stored at -20°C for hours After fixing the cells were stained according to the protocol for the TUNEL ... minutes and the media was aspirated 150 μl of PBS was added, the pellet re-suspended and the total volume transferred to a round bottomed 96 well plate 50 μL of 4% para-formaldehyde was added to the ... minutes and the supernatant aspirated leaving approximately 15 μL in each well The remaining volume was used to resuspend the cells and 200 μL of ice cold 70% ethanol was added The plates were then...
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Báo cáo sinh học: " Inhibition of phosphorylated c-Met in rhabdomyosarcoma cell lines by a small molecule inhibitor SU11274" pdf
... (50-75%) and (> 75%) The score of each slide was the sum of intensity and positive rate scores The staining results were categorized as low expression (the score ≤ 3) and high expression (the score ... μM) for 24 h The noninvasive cells on the upper surface of the membrane were removed with a cotton swab Cells that attached to the lower surface of the membrane and migrated through the Matrigel ... writes up the draft of the manuscript LS and JZ conduct the pathological examination LG and JW conduct trans-well assay JD, YL, and JB provide technique assistance SHH and SJD conceive the study...
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Báo cáo sinh học: "Combination therapy with vemurafenib (PLX4032/RG7204) and metformin in melanoma cell lines with distinct driver mutations" pdf
... phosphorylation and therefore inactivation of the ACC1 isoform of acetyl CoA carboxylase (ACC) [12] or suppression of lipogenic gene expression, including ACC1 and fatty acid synthase, and increase of fatty ... regulator and suppressor of malignant melanoma cell growth, further promoting evidence of expanding the testing of the role of AMPK activation for melanoma treatment Metformin is a derivative of guanidine, ... out of six cell lines with synergistic characteristics, the effect of the combination was greater than the sum of each individual drug effect In the rest there was either an effect due to the...
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Báo cáo hóa học: " Expression of frog virus 3 genes is impaired in mammalian cell lines" pot
... consistent with the absence of 75L expression as a result of a FV3 infection in BGMK cells Therefore, the lack of detectable 75L expression may be a property of the 75L gene and not a defect ... draft the manuscript JM helped perform the research CRB conceived the study and participated in its design and coordination and helped draft the manuscript All authors read and approved the final ... means for further characterization of the function of 75L Competing interests The authors declare that they have no competing interests Authors' contributions HEE performed the research and helped...
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báo cáo hóa học:" Inhibition of phosphorylated c-Met in rhabdomyosarcoma cell lines by a small molecule inhibitor SU11274" pdf
... (50-75%) and (> 75%) The score of each slide was the sum of intensity and positive rate scores The staining results were categorized as low expression (the score ≤ 3) and high expression (the score ... μM) for 24 h The noninvasive cells on the upper surface of the membrane were removed with a cotton swab Cells that attached to the lower surface of the membrane and migrated through the Matrigel ... writes up the draft of the manuscript LS and JZ conduct the pathological examination LG and JW conduct trans-well assay JD, YL, and JB provide technique assistance SHH and SJD conceive the study...
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Báo cáo y học: " DNA methylation patterns associate with genetic and gene expression variation in HapMap cell lines" ppt
... by the number of probe pairs (d) Methylation levels inside and outside of annotation categories, including CpG Islands (CGIs) for probes within 100 bp of the TSS, and histone modifications and ... shared regulatory mechanisms in a fraction of genes However, in the re-analyses of the eQTL data taking into account DNA methylation, in only 10% of eQTLs was the genetic effect of the SNP on expression ... cg07747299 from the HumanMethylation27 array and the nearest CpG island in the region (using Methyl Primer Express from Applied Biosystems) for a total of 411 bp amplified in the 5’ UTR of the C21orf56...
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báo cáo khoa học: "Effect of arginase II on L-arginine depletion and cell growth in murine cell lines of renal cell carcinoma" pptx
... effects on the growth rates of the low arginase producer cell lines CL-2 (P = 0.14) and Renca (P = 0.07) Cell viability of the cells was > 95% at the different points and conditions of the experiments ... to the other cell lines) We then tested the effect of the arginase inhibition by norNOHA (2 mM) on cell proliferation at 24, 48, and 72 hours Growth of CL-19, which had the highest level of arginase ... by the use of DL-α-difluoromethylornithine, which completely blocks the proliferation of these cell lines independent of the presence of L-arginine, L-ornithine and arginase in addition to the...
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top-antitop cross section measurement as a function of the jet multiplicity in the final state and beyond the standard model top-antitop resonances search at the atlas detector at cern
... the decay of the top quark into a W -boson and a b-quark, while the small values of the other elements in the same row suppress the decay of the top quark into the other flavours In fact, the branching ... derivative Dµ includes the coupling of the matter fields with the W and B fields in the kinetic term of the Lagrangian The gauge fields have their kinetic terms added in the gauge part of the Lagrangian ... between them The “top” quark is one of the particles in the Standard Model and it interacts through all kinds of forces in the model: the strong interaction, the weak interaction and the electromagnetic...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008