patient specific modeling of hypoxic response and microvasculature dynamics

... separate fields of CA1, CA2 and CA3 areas and summated for each animal Image analysis and statistical analysis Image J of NIH was used for densitometric analysis of Western blots and MBP expression ... loss, and = cystic infarction and gliosis Eight brain regions (hippocampus: CA1, CA2, CA3, dentate gyrus; anterior and middle regions of cortex; striatum and thalamus) were evaluated, scored, and ... Bourget P, Quinquis V, Fernandez H, Frydman R: Clinical pharmacokinetics of ceftriaxone during the third trimester of pregnancy and study of its transplacental passage in two patients Pathol Biol (Paris)...

Ngày tải lên: 10/08/2014, 10:20

... serum sodium of 111 mmol/litre, low urea of 0.8 mmol/ litre along with low chloride and potassium levels Urinalysis revealed a urine osmolality of 67 mosmol/kg and urinary sodium of 10 mmol/litre ... management of the case, collected and analysed data, and drafted, revised and referenced the manuscript MH had direct participation in management of the case, was involved in critical revision of the ... was obtained from the patient for publication of this case report and any accompanying images A copy of the written consent is available for review by the Editor-in-Chief of this journal Competing...

Ngày tải lên: 11/08/2014, 19:21

... focused on understanding the alterations in GABA content, total GABA and GABAB receptors and GAD expression in the cerebellum and brain stem of hypoxic neonatal rats and the effects of various resuscitations ... by plotting the specific binding of the radioligand on X-axis and bound/free on Y-axis The maximal binding is a measure of the total number of receptors present in the tissue and the equilibrium ... 0.01 when compared to hypoxic group Hypoxic rats- Hx, Hypoxic rats glucose treated - Hx+G, Hypoxic rats oxygen treated - Hx+O, Hypoxic rats glucose and oxygen treated - Hx+G+O, Hypoxic rats epinephrine...

Ngày tải lên: 10/08/2014, 05:21

... miscarriages, and livedo reticularis Materials and Methods We focused on the secondary prevention of stroke with APS, and compared single antiplatelet therapy and a combination of antiplatelet and anticoagulation ... reported the results of the first randomized, double-blind, controlled trial of two different intensities of warfarin treatment on the prevention of recurrent thrombotic events in patients with APS ... examine the effects of these regimens on recurrence of stroke So, the primary endpoint was occurrence of stroke This study was approved by the ethics committee of Tokai University, and prior informed...

Ngày tải lên: 26/10/2012, 09:48

... Effect of acetaminophen and of low intermittent doses of diazepam on prevention of recurrences of febrile seizures J.Pediatr 1995; 126: 991-5 55 Knudsen FU Febrile seizures: treatment and prognosis ... percent of those pediatric seizure patients are diagnosed with febrile seizures, and 20% are diagnosed with afebrile seizures [48-50] Seizures of any type are usually a manifestation of a number of ... under one year of age [34] Of those who experience a second febrile seizure, the risk of recurrence increases 2-fold [35;36] Predictors of recurrent febrile seizures include: a history of focal, prolonged,...

Ngày tải lên: 26/10/2012, 10:03

... decision-making authority of husbands and elderly members [20-22], secondly, through neglect and low prioritization of women’s health issues [23,24] and finally, because of cultural beliefs that ... knowledge of men on maternal and child health issues, their awareness of their wives’ practices and the preferred means of decision-making The objective of the study is to compare men’s knowledge and ... effect of 1.5 and non -response rate of 3%, the estimated sample size was 1,200 men (600 in each of the two groups) in each district [29] This yielded a total of 4,800 men for four intervention and...

Ngày tải lên: 12/02/2014, 19:20

... reporting of patient and program data across all aspects of the integrated package • Support development and implementation of patient tracking and follow-up tools, case finding, referral, and adherence ... all and, if needed, resuscitation ● Prophylactic eye care ● Postnatal follow-up and care within 24-72 hours of birth regardless of place of delivery to support breastfeeding and identify and ... and treatment response if on ART - Age appropriate social and psychological counseling and support addressing adherence, disclosure and grief - TB prevention, diagnosis and treatment - Pain and...

Ngày tải lên: 12/02/2014, 19:20

... group #P < 0.05 compared to the hypoxic and hypoxic + vector groups **P < 0.05 compared to the hypoxic and hypoxic + vector groups ##P < 0.05 compared to the hypoxic + TRAP1-siRNA group (data ... (10 000 cells per well) and the original medium was removed after h of hypoxia Then, 10 lL of CCK-8 solution and 100 lL of DMEM ⁄ F12 medium were added to each well, and the cells were incubated ... intercellular domain of the type tumour necrosis factor receptor [17] On the basis of the sequence of the homologue, TRAP1 was identified as a member of the HSP90 family The ATPase activity of TRAP1 is...

Ngày tải lên: 16/02/2014, 14:20

... inhibition and TRAIL in osteoarthritic disease and chondrocyte apoptosis, and the hypoxic inhibition of cartilage and chondrocyte degeneration Results Macroscopic and radiographic examination of the ... signalling and thus in cell physiology and cellular destruction ROS are known to induce a wide range of responses, depending on cell type and levels of ROS within the cell [23,24] The aims of this ... chondrocyte apoptosis and may suppress the progression of arthritis Materials and methods Induction of OA Beagle dogs (n = 20) with a mean ± SD age of 1.4 ± 0.4 years and a mean ± SD weight of 10.2 ± 1.4...

Ngày tải lên: 18/02/2014, 14:20

... cDNA was cloned into the XhoI and BamHI sites of pET-15b (B) Diagram of the expressed control HSP27 and PEP-1–HSP27 fusion proteins Each contains a His tag consisting of six histidine residues Expression ... of PEP-1–HSP27 against brain ischemia J J An et al B b d B a b c A A a c (kDa) d 150 75 50 37 25 3 Fig Expression and purification of the PEP-1–HSP27 fusion protein Protein extracts of cells and ... was a major component of the total soluble proteins in cells, and was found to be nearly homogeneous and more than 95% pure by SDS–PAGE analysis The identity of the expressed and purified PEP-1–HSP27...

Ngày tải lên: 18/02/2014, 17:20

... Namutumba and Jinja Busoga region is composed of 11 principalities of the Basoga tribe and is one of the largest traditional kingdoms of Uganda Study II, III, and the health facility component of IV, ... size on one hand and the gender of the child, antenatal care visits, marital status, area of residence and geographical area of residence on the other hand (p

Ngày tải lên: 19/02/2014, 22:20

... demographic and historical features of the patient, measures of the tempo and acuity of the presenting illness, and indicators of the extent of myocardial ischemia and necrosis Since patients with ... classified as markers of necrosis, markers of ischemia and markers of inflammation The features of an ideal cardiac marker would be:       High sensitivity and specificity Rise and fall rapidly ... Research turns towards isolation of and development of assays for sarcomeric proteins Myosin light chains were originally isolated and then subsequently abandoned because of specificity issues Troponin...

Ngày tải lên: 20/02/2014, 08:20

... staining of total cellular and chromatin-bound PCNA T24 were grown and incubated on coverslips Two sets of hypoxic cells and of cells reoxygenated for 10 and 30 were prepared One set of cells ... renewal before and at the end of hypoxic gassing as well as under normoxic conditions As shown in Fig MCM3 and Cdc6 are not, and MCM2 and Cdk2 are barely, detectable on chromatin of starved T24 ... homogenous population of conveniently small subcellular entities During a hypoxic period of 6–7 h, a large amount of them is arrested in the hypoxic preinitiation state’ [6,7] and can be released...

Ngày tải lên: 21/02/2014, 00:20

... domain of neonatal care it is in the form of knowledge about artifacts In the domain of meteorology it is in the form of knowledge of what is important For example, changes in wind speeds and directions ... types of time series data Figure Output of our system with limit = 10 BP is stable around 30 kpa until 5:59:59 Figure Output of our system with limit = 30 Figures and show example output of our ... with our software, to get general feedback on how useful the summaries are These would be performed off-ward, and would involve a small number of doctors looking at generated summaries and suggesting...

Ngày tải lên: 22/02/2014, 02:20

... truncated form of polymerase d with an apparent molecular mass of 116 kDa, approximately the same size as the lower band of polymerase d detected by us The ratio of the intensities of both bands significantly ... Fig Sensitivity of minichromosomes of hypoxic and re-oxygenated cell cultures to single-stranded DNA-specific nuclease P1 Aliquots of SV40 minichromosomes of hypoxic or re-oxygenated cells were ... by single-stranded DNA SV40 minichromosomes of hypoxic and re-oxygenated cell cultures were eluted as described above After sedimentation of the nuclei, the supernatant was divided and one half...

Ngày tải lên: 22/02/2014, 04:20

... levels of striatal anandamide with an increased activity of N-acylposphatidylethanolamine-hydrolyzing phospholipase D and reduced activity and expression of FAAH Both the accumulation of anandamide ... phospholipase D and diacylglycerol (DAG) lipase [8] Because of their lipid solubility, AEA and 2-AG cannot be stored in vesicles and therefore they are synthesized on demand and travel, in a ... amounts of nitric oxide (NO) generated by pathological expression of NO synthase isoforms are certainly neurotoxic, whereas homeostatic levels of NO produced by the endothelial isoform of this...

Ngày tải lên: 07/03/2014, 03:20

... Mechanisms of post-ischemic inflammation Cellular response to injury Post-ischemic inflammation is characterized by a rapid activation of resident microglial cells and by infiltration of neutrophils and ... infarct size and less BBB damage in mouse models of ischemic stroke [87,99,100] The mechanisms of brain damage involve gelatinasemediated disruption of the BBB integrity, of edema and hemorrhagic ... late responses may represent endogenous mechanisms of recovery and repair The switch from detrimental to beneficial effects seems to depend on the strength and the duration of the insult and is...

Ngày tải lên: 07/03/2014, 03:20

... phosphorylation of p100 by NF-jB-inducing kinase and of IKK1 In the alternative pathway, p52 mostly dimerizes with RelB in response to a limited number of stimuli such as lympotoxin B, CD40 ligand and B-cell ... translocation and DNA binding Among the members of the NF-jB family, only p65, c-Rel and RelB are directly able to activate the transcription of target genes The transcriptional capacities of p50 and p52, ... [29,32–34,36] and was related to the downregulation of NF-jB-mediated transcription of the B-cell lymphoma (Bcl-2) family of anti-apoptotic genes [29,36] The apoptosis of cells deprived of NF-jB activity...

Ngày tải lên: 07/03/2014, 03:20

... and cerebral pathophysiology of stroke patients, and neuroprotection should be evaluated on a long-lasting and functional basis, rather than on an acute and histological basis Also, careful and ... comprising waves of release of cytotoxic inflammatory products, cell death and recruitment ⁄ activation of blood or bystander immune cells Eventually, the neuroimmune response causes collapse of the structures ... synthesis and release of proinflammatory mediators [17] It is now widely accepted that the latter are responsible for disruption of the 40 capillary basal lamina, opening of the BBB and infiltration of...

Ngày tải lên: 07/03/2014, 03:20