passage enrichment and isolation of glial precursor cell populations

... differentiation of GFRα1a but not GFRα1b transfected cells 60 v 4.3.3 Cell proliferation profiles of GFRα1a and GFRα1b transfected cells were similar 64 4.3.4 Kinetic differences in the activation of Erk1/Erk2 ... a wide variety of rodent and primate models of Parkinson’s disease (PD) It promotes the survival of a broad range of central and peripheral neurons and is essential for kidney and enteric nervous ... roles of GFRα1a and GFRα1b are unknown Further more, the distribution and expression levels of these two isoforms have not been reported To understand the physiological and functional role of GFRα1a...

Ngày tải lên: 05/10/2015, 22:31

... clonal expansion of T-cells bearing human Vb3, Vb12, Vb17 and Vb19 [20] Others showed proliferation of human T-cells bearing Vb1, Vb2, Vb3, Vb5.2, Vb12, Vb15 and Vb17 domains and found differences ... pathologies and because of the potential use of the toxins as biological weapons Alteration of their MHC and TCR binding capacity by site directed mutagenesis could be useful in the development of vaccines ... hVb2.1hCb2 and hVb1hCb2 (genes kindly provided by U Utz and R P Sekaly, University of Montreal, Canada), were cloned between the NdeI and EcoRI restriction sites of the pET17b expression vector and...

Ngày tải lên: 07/03/2014, 16:20

... of FtsZ occurred spontaneously at urea concentrations > 0.6 m and > 1.25 m in the absence and presence of 0.6 m TMAO, respectively Furthermore, the standard free energy changes of unfolding of ... increasing concentration of urea in the absence and presence of TMAO The Dm values for the urea-induced unfolding of FtsZ were found to be 1.8 and 3.6 m in the absence and presence of TMAO, respectively ... absence and presence of 0.8 m TMAO, respectively Electron microscopy analysis of the FtsZ assembly reaction showed the formation of thicker and larger bundles of FtsZ protofilaments in the presence of...

Ngày tải lên: 30/03/2014, 16:20

... subsides, a minor subset of T cells down-regulate PD-1 and become memory cells, while the larger pool of effector cells extinguishes through a range of mechanisms leading to cellular apoptosis Conversely, ... properties of nonreplicating vectors and biological response modifiers by direct intra-nodal administration of plasmid and peptide [19,41] We showed that the sequence and the route of administration of ... robust expansion of epitope-specific CD8+ T cells (on the order of 1/2 - 1/10 specific T cells/total CD8+ T cells), reversing the order of the vectors resulted in a limited overall T cell expansion...

Ngày tải lên: 18/06/2014, 16:20

... a somatic cell hybrid panel and the isolation of homologous probes from large insert genomic DNA libraries In addition, some difculties remained with the nomenclature of BTA25, BTA27 and BTA29 ... ambiguities of the bovine karyotype nomenclature, particularly concerning BTA25, BTA27 and BTA29, and could be used as a standard for cytogeneticists using different banding techniques (G, R and Q) ... Pollock D.L., Gustavsson I., Proceedings of the First International Conference for the Standardization of banded karyotypes of Domestic Animals, Reading, England 1976, Hereditas 92 (1980) 145162...

Ngày tải lên: 09/08/2014, 18:21

... time course of the motility and the localization of the aspartate receptor protein (Tar) for several cell generations We also report cellular responses caused by the addition and removal of the aspartate ... single cell observation, image analysis and drafted the manuscript DS and IK prepared Tar-GFP vector and discussed this study KY conceived of the study, and participated in its design and coordination ... frequency and the protein-localization dynamics When the cell divided into two daughter cells, one of these two daughter cells was picked up by optical tweezers, transported to the axle area and was...

Ngày tải lên: 11/08/2014, 00:22

... HYW and QT participated in the serological studies, virus isolation, and the design of the study GDL conceived of the study, and participated in its design and coordination All authors read and ... virus isolation include both male and female with age range from 0.5 to 37 years old (Table 1), and resided in southwestern (Yunnan and Guizhou province) and northwest areas (Gansu province) of ... careful review of the manuscript and useful suggestions This work was supported by grants from the Ministry of Science and Technology, People’s Republic of China (no.2003BA712A08-01 and 2009ZX10004-202);...

Ngày tải lên: 12/08/2014, 02:20

... regions of tagged line ET2-17 and RT-PCR analysis of their regulation of the luc+ Cis-acting Cis-acting elements present in the 5'-tagged regions of tagged line ET2-17 and RT-PCR analysis of their ... out of 10 LT-responsive lines This result demonstrates reliability of the simple and fast qualitative scoring of recorded images, and suggests developmental regulation of LT upregulation of the ... exposure times of up to 20 Isolation and characterization of T-DNA flanking sequences Isolation of T-DNA flanks was accomplished with Thermal Asymmetric Interlaced-PCR (TAIL-PCR) and Inverse PCR...

Ngày tải lên: 12/08/2014, 03:20

... Simulations HCP and SCP β -cell clusters of different sizes with number of β-cells nβ ranging from 1–343, number of inter β -cell couplings of each β -cell nc varying between 0–12, and coupling strength ... d, and f) on the gc-nc plane λ, and bursting period Tb plotted for a HCP-323 β -cell cluster (a, c and e) and Figure burster cells fb, synchronization index Fraction of Fraction of burster cells ... periodogram Cell sorting using Lomb-Scargle periodogram (a) Calcium profiles, (b) membrane action potential profiles, and (c) Lomb-Scargle periodogram, of a burster cell, a spiker cell and a silent cell...

Ngày tải lên: 13/08/2014, 16:21

... Ratio of inner cell mass and trophoblastic cells in blastocysts derived from porcine 4- and 8cell embryos and isolated blastomeres cultured in vitro in the presence or absence of protein and human ... system Stem cell research: social and political factors Public sharing of information about the basic science of ESCs has proven to be important, since those who are aware of the stem cell debate ... scientifically sound, legal, and ethical, the NIH will examine the derivation of all existing stem cell lines and create Page of (page number not for citation purposes) Theoretical Biology and Medical Modelling...

Ngày tải lên: 13/08/2014, 23:20

... electronics The philosophy of UPEC cell is to design a standard PEBB cell, which is capable of implementing different kind of operation modes This thesis studies the effect of input and output filter ... of basic UPEC cell: AC/DC operation Topology of basic UPEC cell: DC/AC operation Topology of soft-switching UPEC cell: DC/DC operation 2 2.1 2.2 2.3 2.4 2.5 2.6 Dataflow graph of ... 1.6: Topology of soft-switching UPEC cell: DC/DC operation 1.4 Scope of Thesis The main focus of this thesis is to look into design of UPEC cell for AC/DC operation Since the idea of UPEC requires...

Ngày tải lên: 29/09/2015, 12:52

... units Standardization of cells and their connectivity will lead to reduction of engineering costs in power electronic systems Once standardized and produced in large numbers, the cost of this cells ... programming flow chart and the comparison of simulated and experimental results of the cell Chapter and Chapter form control part of the thesis These two chapters analyze the possibilities of building the ... switching frequency and prevent reduction in the size and cost of passive components and limit the bandwidth of control loops For IGBTs, the turn-off loss is dominant because the turn-off current switching...

Ngày tải lên: 29/09/2015, 12:55

... Synchronisation of HuH7 and HepG2 cells 40 4.2 Total RNA extraction and mature miRNA expression profiling 43 4.3 Effects of miRNA on cell proliferation 52 4.3.1 Determination of concentration of miRNA ... 24 3.2 Cell culture 25 3.3 Synchronisation of cells 25 3.4 Cell cycle analysis 26 3.5 RNA extraction and quantitation 26 3.6 Mature miRNA expression profiling 27 3.7 Effects of miRNAs on cell proliferation ... transfection of miRNA mimics and inhibitors in (A) HuH7 and (B) HepG2 cells 56 Figure 4.8 Transfection of mimic miR-193a and mimic miR-210 delays HuH7 cell cycle progression 58 Figure 4.9 Expression of...

Ngày tải lên: 05/10/2015, 22:31

... Synchronisation of HuH7 and HepG2 cells 40 4.2 Total RNA extraction and mature miRNA expression profiling 43 4.3 Effects of miRNA on cell proliferation 52 4.3.1 Determination of concentration of miRNA ... 24 3.2 Cell culture 25 3.3 Synchronisation of cells 25 3.4 Cell cycle analysis 26 3.5 RNA extraction and quantitation 26 3.6 Mature miRNA expression profiling 27 3.7 Effects of miRNAs on cell proliferation ... transfection of miRNA mimics and inhibitors in (A) HuH7 and (B) HepG2 cells 56 Figure 4.8 Transfection of mimic miR-193a and mimic miR-210 delays HuH7 cell cycle progression 58 Figure 4.9 Expression of...

Ngày tải lên: 06/10/2015, 20:35

... Swiss Tropical Institute and University of Basel for making this Joint MSc program possible In particular I thank Prof Markus Wenk and Prof Vincent Chow My acknowledgment to Prof Gerd Pluschke for ... to detect binding of phage to DENV-2 TSV01 whole virus and EDIII protein……………………………… ………42 2.8 Determination of replication, dissemination and viremia titres of DENV-2 EDEN and PDK-53 strains ... Characterization of Serum from Subject A 3.2.1 Expression and Purification of DENV-2 and -3 Ectodomain III (EDIII) …………………………………………………………….…48 3.2.2 Propagation of DENV-2 TSV01, DENV-3 H87, DENV-1, 2, and...

Ngày tải lên: 13/10/2015, 16:41

... and the origin of Italian populations of T destruens and T piniperda, by genetic markers of mtDNA The central geographic position of Italy in the Mediterranean could be important for understanding ... et al Figure Sampling sites of Italian populations of T destruens (ITA1-4) and locations of the populations used for the phylogeographic analysis The natural range of the potential host pine species ... sympatry of T destruens and T piniperda in Italian transition areas between continental and Mediterranean pine forests MATERIALS AND METHODS 2.1 Sample collection and DNA isolation Adults of Tomicus...

Ngày tải lên: 08/08/2014, 00:22

... polygenic value of the offspring was calculated as: A offspring = / A sire + / A sire + a offspring Where aoffspring is the Mendelian sampling term for the offspring and was randomly sampled ... the offspring has probability ¾ of receiving two randomly selected recipient line alleles with a probability of identity by descent (IBD) of 0.2, and probability 1/4 of receiving one recipient and ... descent (IBD) of 0.2, and 1/2 chance of receiving one recipient and one donor allele with a probability of being IBD, so the offspring has F = 0.1 It has one parent with F = 0.0 and the other...

Ngày tải lên: 14/08/2014, 13:21

... Switzerland), thermolysin, chymotrypsin or elastase (Sigma, St Louis, MO, USA)] and Cloning of the bovine MUC15 cDNA by PCR with degenerate primers Isolation of total RNA from the mammary gland of ... database, and a similar sequence was located The milk cell fraction of lactating tissue contains bud-off epithelial cells, enabling performance of an indirect assay for expression of this possible ... PCR screening of bovine and human cDNA libraries showed the presence of MUC15 mRNA in human breast, bovine mammary gland, bovine lymph nodes and lungs of both species (Table 1) Of the identified...

Ngày tải lên: 24/03/2014, 04:21

... APPsa and APLP2s secretion to 60 and 75%, respectively, of untreated cells (Fig 2A,B, lanes and 3) and reduced the PMA-stimulated amount of both shed ectodomains to almost the level of control cells ... Proteolytical processing of APP and APLP2 in RA-treated neuroblastoma cells Western blots and quantification of (A) APLP2s and of (B) APPsa in RA-differentiated neuroblastoma cell lines Cells were treated ... of RA-induced differentiation of neuroblastoma cell lines on ADAM10 and TACE expression and on the release of secreted APLP2 and APPsa For neuronal (N)-type SH-SY5Y cells, differentiation by RA...

Ngày tải lên: 30/03/2014, 11:20

... Isolation and characterization of stem cell regulatory genes oct4 and stat3 from the model fish medaka LIU RONG (Master of Biological Sciences) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF ... eukaryotic cells and expression analysis in vitro 60 2.2.5.1 Construction of plasmid of DNA 60 2.2.5.2 Cell culture 61 2.2.5.2.1 Culture conditions 61 2.2.5.2.2 Freezing and thawing of the cells 62 ... Developmental potency of stem cells Stem cells are unspecialized cells that have the capability of self-renewal for producing identical unspecialized daughter cells and the potential of differentiation...

Ngày tải lên: 14/09/2015, 13:38