... quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 Page R & Stevens RC (2004) Crystallization data mining in structural genomics: using positive and ... possibility is that it participates in forming heterooligomers containing subunits of both PRTFDC1 and HPRT, thereby providing an additional means of regulating the activity of HPRT Recently, Suzuki et ... in midpoint ofthe aggregation process measured by DSLS) were PRPP, GMP and IMP Large increase in thermal shift is an indication of protein-compound binding The high thermal shifts for GMP, and...
... affected by the inhibitors ofthe enzymatic activity Therefore the iodine staining method is well suited for extensive kinetic analyses of purified b-amylases substrate [21] The inhibition type of glucose, ... ORF of 499 amino acids with one putative initiation codon at position ofthe deduced amino-acid sequence (Fig 3) Translation starting with this methionine residue results in a protein of 498 amino ... As the methionine at position is the first amino acid the residue preceding this methionine is shown in lower case The sequence corresponding to the N-terminal sequence ofthe protein is underlined...
... position in the complete sequence of Cyn d 24 is indicated the amino acids of number and number peptide sequences (Fig 2B), and an antisense primer, designed on the basis ofthe amino acids ofthe ... Chen (Institute of Biochemistry, Academia Sinica) for performing the oligosaccharide analysis This work was supported in part by Promotion of Research-oriented university program from the Ministry ... et al isoforms and these have high pI values ranging from 9.5 to 10.5 [10–15] Similarly, Cyn d is a calcium-binding protein containing two Ca2+, and its allergenicity and cross-reactivity have...
... 2004 Purification andstructural study ofhuman PKIb (Eur J Biochem 271) 1771 Fig Activity analysis of inhibition of cAMP-dependent protein kinase activity by inhibitor peptides (A) The inhibitory ... single band by SDS/PAGE (Fig 2) The assay of its activity demonstrated that the purified PKIb inhibited the catalytic subunit of cAPK with the specific activity of 6.0 · 104 unitÆmg)1 (Fig 3A) .The ... through incubation, increasing concentration ofhuman PKIb with cAPK (B) Kinetic analysis of inhibition of cAMP-dependent protein kinase activity by inhibitor peptides Assays were performed essentially...
... sets of data gives an indication ofthe role of Zn(II) in the Hg(II)-containing species formed at physiological pH The use of two different Hg(II) salts allowed analysis ofthe possible role ofthe ... Zinc (II), cadmium ¨ (II), and mercury (II) thiolate transitions in metallothionein Biochemistry 20, 2852–2856 16 Jonhson, B.A & Armitage, I. M (1987) Equilibrium and kinetic analysis ofthe interaction ... analysis ofthe difference UV-vis data, which discloses the effect of each Hg(II) addition, can give an insight into the evolution ofthe co-ordination geometry about Hg(II) in the MT species formed...
... subtilisin between the prodomain andthe target protein, thus releasing the target protein in its native form, beginning with the initiator methionine Cloning of Rv0948c and MT0975 genes D Fig ... University) and 100 ng of primers were used in the amplification The amplified DNA obtained with oligonucleotide pairs 1, and was digested with DraI and EcoRI for cloning into the respective sites ... protein with the subtilisin prodomain The fusion protein would be bound to a resin coupled with a stable variant of subtilisin protease Next, equilibration with fluoride anion will trigger the...
... immunodecorated with Ndh antibodies The position ofthe immune reaction for the Ndh antibodies is indicated on the side ofthe blots The experiments were performed in identical conditions using BS chloroplasts, ... reaction was performed by incubation ofthe membranes with a mixture of primary antibodies (NdhE, -H, -J and -K) in a dilution of : 1000 Before a mixture of Ndh antibodies was used, their individual ... mass standards and direction of migration are indicated for both the first (1D) and second (2D) dimension The position ofthe Ndh complex was detected in the square containing the protein complexes...
... loop) interact with the membrane interface ofthe PC ⁄ PIP2 vesicle as an auxiliary membrane interacting site in addition to the PIP2 specific-high affinity lipid binding site consisting ofthe b1 ... structure III to structure II Because structure II and structure III are in equilibrium at the surface of PC ⁄ PS ⁄ PIP2 vesicles, there might be a critical level of structure II required for the restoration ... lipids between the inner and outer leaflets ofthe membrane and a lateral segregation of lipid components in the membrane may also cause fluctuations ofthe lipid composition in the vicinity of...
... affected in a similar way Electrostatic interactions play a critical role in guiding and stabilizing functional protein–protein interactions [6], as well as in thestructural stability of proteins ... ± stability ofthe protein, whereas inversion ofthe charge at position 72 considerably reduces the stability against urea-induced unfolding, i. e electrostatic interactions ofthe negative charge ... wild-type protein Inspection ofthe values obtained for the Gibbs energies of unfolding ofthe mutant forms of cyt c6 revealed significant nonadditive effects in the stabilities ofthe double mutants...
... CONTENTS iv SUMMARY vii LIST OF TABLES ix LIST OF FIGURES xi LIST OF SCHEMES xv LIST OF ABBREVIATIONS xvii CHAPTER INTRODUCTION 1.1 Overview 1.2 Definition, Classification and sources of Natural ... sufficient quantities and total synthesis is not feasible due to high complexity ofthe molecule, a hybrid approach is utilized Semisynthesis ofthe target molecule is applied by utilising the biosynthetic ... with Anti-inflammatory Activity Inflammation is the protective response towards noxious stimuli and conditions such as infection, tissue injury and irritants.25 The word inflammation is derived...
... characterization ofthe Nogo-A functional domains (Eur J Biochem 271) 3513 Fig Schematic representation ofthe domain organization ofthehuman Nogo-A protein (A) The domain organization ofhuman ... after its intrinsic helix-forming propensity is stabilized by the introduction ofthe helix-stabilizing solvent trifluoroethanol We report here the structure of Nogo-40, a Nogo-66 Fig Expression and ... hand, it is highly probable that the N-terminal positive part is responsible for its binding to the NgR negative cavity This is in complete agreement with previous findings that deletion of the...
... are the mean ± standard deviation of at least three experiments Tm is the midpoint ofthe thermal transition unfolding; DHcal is the calorimetric enthalpy ofthe whole transition Tm2 is the main ... to the midpoint ofthe thermal transition unfolding, was determined from the derivative ofthe transition curve Curve fitting was performed using ORIGIN (Microcal Software) Differential scanning ... alterations in Ca2+ sensitive regulation of actomyosin S1 Mg2+ ATPase activity in the presence of troponins In this condition, the tropomyosin–troponin complex inhibits or activates the actomyosin...
... regulation of channel properties The tissue distributionofthehuman b1B subunit is similar to that ofthehuman b1 subunit Its message was detected in skeletal muscle and in a variety of subregions ... subunit via the extension of exon to intron (or partial intron retention) with an in-frame stop codon Tissue distributionofthehuman b1B subunit Northern blot analysis, using a human b1B specific ... current densities were increased by two- and threefold, respectively (Fig 4G) Despite significant variability in current densities within and between batches of oocytes, statistical analysis indicated...
... antibiotic peptide in the secretion oftheskin glands of L citropa [9] It is one ofthe most potent membrane-active antibiotic peptides isolated from amphibians and is particularly effective ... Results Biological testing The antibiotic activities [as minimum inhibitory concentration (MIC) values in lgÆmL)1] of two natural citropins (1.1 and 1.1.2) and 23 synthetic modifications of citropin ... hydrophilic residues to Lys (to determine the effect on activity of an increase in the positive charge ofthe peptide) The spectrum of antibiotic activities for each synthetic modification is recorded...
... a-helical acidic domain contained within the first 72 amino acids, the Ets domain, the CIDD andthe C-terminal domain The fragment of cDNA included in the bait used for two-hybrid screening ofthe ... deletions of CHD3 on the inhibition of transcription by CHD3 and on the binding of CHD3 with ERM (A) Effect of N-terminal deletions on the inhibition of transcription by CHD3 SH-SY5Y cells were transiently ... activation 3-Amino-1,2,4-triazole (3-AT; an inhibitor of histidine biogenesis) is used to quench background growth on His minus medium andthe minimum level of 3-AT required varies with the bait Library...
... by human AChR antibodies in MG sera, allowing their immediate use for the detailed study ofthe specificities ofthe antibodies in MG sera 3564 andthe development of antigen-specific therapeutic ... subunit andthe c ⁄ e subunits andthe other between the second a subunit andthe d subunit [4] The c ⁄ e and d subunits play a major role in shaping the ligand-binding sites and also in maintaining ... BSA–Sepharose resin, then measured the nonbound AChR antibodies in the initial and final samples If the AChR antibodies in the MG serum recognized and bound to the recombinant, immobilized proteins, there...
... study, in view ofthe reported discrepancies in the patterns of exon usage andthe presence of multiple transcription initiation (TI) sites within thehuman TP gene [4,6,7], the initial aim was ... TI sites clustered at sites within E1 and E1b RESULTS Analysis of 5¢ UTR of TPa and TPb Identification ofthe major TP mRNA transcripts and determination of their transcription initiation sites ... (Eur J Biochem 269) Ó FEBS 2002 Fig Determination ofthe transcription initiation sites for the TP gene (A) Relative positioning ofthe oligonucleotide primers used for the amplification ofthe 5¢...
... ofthe third range, their influence on the morphofunctional characteristics ofthe femur as a total is mini- mal, and that their absolute meaning characterizes the individual variability in the ... phenomenon is the result ofthe evolution ofthe locomotor apparatus, encompassing constitutional and individual variability This investigation studied the organization ofthehuman femur as a system of ... J: Qualitative evaluation of titanium implant integration into bone by diffraction enhanced imaging Phys Med Biol 2006, 51:1313-1324 Nikitiuk IE, Ovsiankin NA: The differential diagnosis of posttraumatic...
... theories ofthe transmission of light and sound; ofthe production of winds, and sun-spots, andofthe method of development and dissemination of heat, are in point of fact, unphilosophical and incomprehensible ... rapidity, and with varying and sometimes with striking results In the extremes of atmospheric temperature, this fluid is found to exist in the extremes of its positive and negative conditions The ... difference in the medium of communication, which is in either case the vito-magnetic fluid; but that in the former the normal fluid is affected simply, while in the latter an artificial and extraordinary...
... Arg148 is not the only buried arginine in the structure of BLG The whole side chain of Arg40 (the other main point of trypsin action) in the native structure ofthe protein is deeply buried inside ... to the action of trypsin The nature ofthe interactions in this region, as pointed out above, also explains the reversibility of temperature-induced dissociation ofthe BLG dimer Although this ... BLG ratios (1 : 10 and : 20) and at the highest temperature compatible with retention of enzyme activity and with the reversibility ofstructural modifications of BLG Limited proteolysis studies...