part i physical and structural distribution of the human skin

... quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 Page R & Stevens RC (2004) Crystallization data mining in structural genomics: using positive and ... possibility is that it participates in forming heterooligomers containing subunits of both PRTFDC1 and HPRT, thereby providing an additional means of regulating the activity of HPRT Recently, Suzuki et ... in midpoint of the aggregation process measured by DSLS) were PRPP, GMP and IMP Large increase in thermal shift is an indication of protein-compound binding The high thermal shifts for GMP, and...

Ngày tải lên: 15/02/2014, 01:20

... affected by the inhibitors of the enzymatic activity Therefore the iodine staining method is well suited for extensive kinetic analyses of purified b-amylases substrate [21] The inhibition type of glucose, ... ORF of 499 amino acids with one putative initiation codon at position of the deduced amino-acid sequence (Fig 3) Translation starting with this methionine residue results in a protein of 498 amino ... As the methionine at position is the first amino acid the residue preceding this methionine is shown in lower case The sequence corresponding to the N-terminal sequence of the protein is underlined...

Ngày tải lên: 22/02/2014, 07:20

... position in the complete sequence of Cyn d 24 is indicated the amino acids of number and number peptide sequences (Fig 2B), and an antisense primer, designed on the basis of the amino acids of the ... Chen (Institute of Biochemistry, Academia Sinica) for performing the oligosaccharide analysis This work was supported in part by Promotion of Research-oriented university program from the Ministry ... et al isoforms and these have high pI values ranging from 9.5 to 10.5 [10–15] Similarly, Cyn d is a calcium-binding protein containing two Ca2+, and its allergenicity and cross-reactivity have...

Ngày tải lên: 07/03/2014, 12:20

... 2004 Purification and structural study of human PKIb (Eur J Biochem 271) 1771 Fig Activity analysis of inhibition of cAMP-dependent protein kinase activity by inhibitor peptides (A) The inhibitory ... single band by SDS/PAGE (Fig 2) The assay of its activity demonstrated that the purified PKIb inhibited the catalytic subunit of cAPK with the specific activity of 6.0 · 104 unitÆmg)1 (Fig 3A) .The ... through incubation, increasing concentration of human PKIb with cAPK (B) Kinetic analysis of inhibition of cAMP-dependent protein kinase activity by inhibitor peptides Assays were performed essentially...

Ngày tải lên: 07/03/2014, 15:20

... sets of data gives an indication of the role of Zn(II) in the Hg(II)-containing species formed at physiological pH The use of two different Hg(II) salts allowed analysis of the possible role of the ... Zinc (II), cadmium ¨ (II), and mercury (II) thiolate transitions in metallothionein Biochemistry 20, 2852–2856 16 Jonhson, B.A & Armitage, I. M (1987) Equilibrium and kinetic analysis of the interaction ... analysis of the difference UV-vis data, which discloses the effect of each Hg(II) addition, can give an insight into the evolution of the co-ordination geometry about Hg(II) in the MT species formed...

Ngày tải lên: 16/03/2014, 18:20

... subtilisin between the prodomain and the target protein, thus releasing the target protein in its native form, beginning with the initiator methionine Cloning of Rv0948c and MT0975 genes D Fig ... University) and 100 ng of primers were used in the amplification The amplified DNA obtained with oligonucleotide pairs 1, and was digested with DraI and EcoRI for cloning into the respective sites ... protein with the subtilisin prodomain The fusion protein would be bound to a resin coupled with a stable variant of subtilisin protease Next, equilibration with fluoride anion will trigger the...

Ngày tải lên: 17/03/2014, 17:20

... immunodecorated with Ndh antibodies The position of the immune reaction for the Ndh antibodies is indicated on the side of the blots The experiments were performed in identical conditions using BS chloroplasts, ... reaction was performed by incubation of the membranes with a mixture of primary antibodies (NdhE, -H, -J and -K) in a dilution of : 1000 Before a mixture of Ndh antibodies was used, their individual ... mass standards and direction of migration are indicated for both the first (1D) and second (2D) dimension The position of the Ndh complex was detected in the square containing the protein complexes...

Ngày tải lên: 23/03/2014, 13:20

... loop) interact with the membrane interface of the PC ⁄ PIP2 vesicle as an auxiliary membrane interacting site in addition to the PIP2 specific-high affinity lipid binding site consisting of the b1 ... structure III to structure II Because structure II and structure III are in equilibrium at the surface of PC ⁄ PS ⁄ PIP2 vesicles, there might be a critical level of structure II required for the restoration ... lipids between the inner and outer leaflets of the membrane and a lateral segregation of lipid components in the membrane may also cause fluctuations of the lipid composition in the vicinity of...

Ngày tải lên: 30/03/2014, 10:20

... affected in a similar way Electrostatic interactions play a critical role in guiding and stabilizing functional protein–protein interactions [6], as well as in the structural stability of proteins ... ± stability of the protein, whereas inversion of the charge at position 72 considerably reduces the stability against urea-induced unfolding, i. e electrostatic interactions of the negative charge ... wild-type protein Inspection of the values obtained for the Gibbs energies of unfolding of the mutant forms of cyt c6 revealed significant nonadditive effects in the stabilities of the double mutants...

Ngày tải lên: 30/03/2014, 20:20

... CONTENTS iv SUMMARY vii LIST OF TABLES ix LIST OF FIGURES xi LIST OF SCHEMES xv LIST OF ABBREVIATIONS xvii CHAPTER INTRODUCTION 1.1 Overview 1.2 Definition, Classification and sources of Natural ... sufficient quantities and total synthesis is not feasible due to high complexity of the molecule, a hybrid approach is utilized Semisynthesis of the target molecule is applied by utilising the biosynthetic ... with Anti-inflammatory Activity Inflammation is the protective response towards noxious stimuli and conditions such as infection, tissue injury and irritants.25 The word inflammation is derived...

Ngày tải lên: 09/09/2015, 11:26

... characterization of the Nogo-A functional domains (Eur J Biochem 271) 3513 Fig Schematic representation of the domain organization of the human Nogo-A protein (A) The domain organization of human ... after its intrinsic helix-forming propensity is stabilized by the introduction of the helix-stabilizing solvent trifluoroethanol We report here the structure of Nogo-40, a Nogo-66 Fig Expression and ... hand, it is highly probable that the N-terminal positive part is responsible for its binding to the NgR negative cavity This is in complete agreement with previous findings that deletion of the...

Ngày tải lên: 16/03/2014, 16:20

... are the mean ± standard deviation of at least three experiments Tm is the midpoint of the thermal transition unfolding; DHcal is the calorimetric enthalpy of the whole transition Tm2 is the main ... to the midpoint of the thermal transition unfolding, was determined from the derivative of the transition curve Curve fitting was performed using ORIGIN (Microcal Software) Differential scanning ... alterations in Ca2+ sensitive regulation of actomyosin S1 Mg2+ ATPase activity in the presence of troponins In this condition, the tropomyosin–troponin complex inhibits or activates the actomyosin...

Ngày tải lên: 16/03/2014, 18:20

... regulation of channel properties The tissue distribution of the human b1B subunit is similar to that of the human b1 subunit Its message was detected in skeletal muscle and in a variety of subregions ... subunit via the extension of exon to intron (or partial intron retention) with an in-frame stop codon Tissue distribution of the human b1B subunit Northern blot analysis, using a human b1B specific ... current densities were increased by two- and threefold, respectively (Fig 4G) Despite significant variability in current densities within and between batches of oocytes, statistical analysis indicated...

Ngày tải lên: 16/03/2014, 23:20

... antibiotic peptide in the secretion of the skin glands of L citropa [9] It is one of the most potent membrane-active antibiotic peptides isolated from amphibians and is particularly effective ... Results Biological testing The antibiotic activities [as minimum inhibitory concentration (MIC) values in lgÆmL)1] of two natural citropins (1.1 and 1.1.2) and 23 synthetic modifications of citropin ... hydrophilic residues to Lys (to determine the effect on activity of an increase in the positive charge of the peptide) The spectrum of antibiotic activities for each synthetic modification is recorded...

Ngày tải lên: 17/03/2014, 09:20

... a-helical acidic domain contained within the first 72 amino acids, the Ets domain, the CIDD and the C-terminal domain The fragment of cDNA included in the bait used for two-hybrid screening of the ... deletions of CHD3 on the inhibition of transcription by CHD3 and on the binding of CHD3 with ERM (A) Effect of N-terminal deletions on the inhibition of transcription by CHD3 SH-SY5Y cells were transiently ... activation 3-Amino-1,2,4-triazole (3-AT; an inhibitor of histidine biogenesis) is used to quench background growth on His minus medium and the minimum level of 3-AT required varies with the bait Library...

Ngày tải lên: 30/03/2014, 09:20

... by human AChR antibodies in MG sera, allowing their immediate use for the detailed study of the specificities of the antibodies in MG sera 3564 and the development of antigen-specific therapeutic ... subunit and the c ⁄ e subunits and the other between the second a subunit and the d subunit [4] The c ⁄ e and d subunits play a major role in shaping the ligand-binding sites and also in maintaining ... BSA–Sepharose resin, then measured the nonbound AChR antibodies in the initial and final samples If the AChR antibodies in the MG serum recognized and bound to the recombinant, immobilized proteins, there...

Ngày tải lên: 30/03/2014, 10:20

... study, in view of the reported discrepancies in the patterns of exon usage and the presence of multiple transcription initiation (TI) sites within the human TP gene [4,6,7], the initial aim was ... TI sites clustered at sites within E1 and E1b RESULTS Analysis of 5¢ UTR of TPa and TPb Identification of the major TP mRNA transcripts and determination of their transcription initiation sites ... (Eur J Biochem 269) Ó FEBS 2002 Fig Determination of the transcription initiation sites for the TP gene (A) Relative positioning of the oligonucleotide primers used for the amplification of the 5¢...

Ngày tải lên: 31/03/2014, 09:20

... of the third range, their influence on the morphofunctional characteristics of the femur as a total is mini- mal, and that their absolute meaning characterizes the individual variability in the ... phenomenon is the result of the evolution of the locomotor apparatus, encompassing constitutional and individual variability This investigation studied the organization of the human femur as a system of ... J: Qualitative evaluation of titanium implant integration into bone by diffraction enhanced imaging Phys Med Biol 2006, 51:1313-1324 Nikitiuk IE, Ovsiankin NA: The differential diagnosis of posttraumatic...

Ngày tải lên: 20/06/2014, 00:20

... theories of the transmission of light and sound; of the production of winds, and sun-spots, and of the method of development and dissemination of heat, are in point of fact, unphilosophical and incomprehensible ... rapidity, and with varying and sometimes with striking results In the extremes of atmospheric temperature, this fluid is found to exist in the extremes of its positive and negative conditions The ... difference in the medium of communication, which is in either case the vito-magnetic fluid; but that in the former the normal fluid is affected simply, while in the latter an artificial and extraordinary...

Ngày tải lên: 17/02/2014, 01:20

... Arg148 is not the only buried arginine in the structure of BLG The whole side chain of Arg40 (the other main point of trypsin action) in the native structure of the protein is deeply buried inside ... to the action of trypsin The nature of the interactions in this region, as pointed out above, also explains the reversibility of temperature-induced dissociation of the BLG dimer Although this ... BLG ratios (1 : 10 and : 20) and at the highest temperature compatible with retention of enzyme activity and with the reversibility of structural modifications of BLG Limited proteolysis studies...

Ngày tải lên: 21/02/2014, 15:20