panther gene expression analysis tools

... Cerevisiae genes Gene expression levels are indicated by the heatmap This cluster contains 130 genes The gene expressions in the cdc15 experiment oscillate between low and high levels Most of these genes ... Systems Biology 1: Input gene expression measurements, all sampled genes (referred as Ω), experimentally verified cycle-involved genes (denoted as G), noncycle-involved genes (represented as F) ... in the prior knowledge of the 104 genes The false positive control removed genes and left 722 genes marked as potential cell cycle involved genes The identified genes are provided in the additional...

Ngày tải lên: 22/06/2014, 00:20

... the level of gene expression The two main sources of heterogeneity in gene expression variations — additive and multiplicative components — are excluded in our analysis by eliminating expression ... Differential gene expression analysis of active disease Statistical analysis of the difference of gene expression in samples from patients and 12 healthy controls gave the following results 1716 genes ... of genes that were identified by differential expression analysis, hypervariable (HV) gene analysis, and discriminant function analysis (DFA) are represented in a Venn diagram The numbers of genes...

Ngày tải lên: 09/08/2014, 01:23

... comprising genes with tissue-specific expression and genes with similar or contrasting expression in multiple tissues Category analysis of expression patterns identified genes that were modulated by T3-dependent ... Figure Concordance of gene expression determined by microarrays and RT-PCR Concordance of gene expression determined by microarrays and RT-PCR Expression ratios of representative genes in (a) cerebellum ... expressed genes identifies gene clusters with biological associations Figure Hierarchical clustering of differentially expressed genes identifies gene clusters with biological associations Gene- expression...

Ngày tải lên: 09/08/2014, 20:20

... our unigene dataset indicative of a broad coverage of expressed genes in the flax genome Hierarchical cluster analysis of flax tissue based EST collections In order to compare the gene expression ... a technique which has been widely used in microarray analysis [23] The results are shown in Figure The analysis shows that in general gene expression is most closely related in tissues that are ... together with the generation of ESTs derived from these libraries and the related assembled unigenes We mined the resulting database with the goal of revealing new insights into the gene expression...

Ngày tải lên: 11/08/2014, 11:22

... R3 Selected genes Figure Clustering of genes changing during fruit development Clustering of genes changing during fruit development Cluster analysis of gene expression A and B, Expression patterns ... cell cycle associated genes during this period we identified the genes associated with the cell cycle classification for each cluster (FB 17 genes, EFD 61 genes, MD genes, R 42 genes) and their annotations ... http://www.biomedcentral.com/1471-2229/8/16 A Microarray expression B 50 100 150 C 50 100 150 0 12 10 0 50 100 150 Days after anthesis Figure Expression of core cell cycle genes Expression of core cell cycle genes Array expression levels...

Ngày tải lên: 12/08/2014, 05:20

... -1.45 -1.35 1.33 1.41 1.79 1.86 1.96 2.36 Figure qRT-PCR analysis of gene expression: P43 Nfia-/- versus P43 Nfia+/+ qRT-PCR analysis of gene expression: P43 Nfia-/- versus P43 Nfia+/+ Shown are ... combined with phylogenetic First, NFI-A might alter the expression levels of other transcription factors that affect target gene expression (indirect influence) For example, expression of the ... phylogenetically conserved NFI binding sites in their promoter region, suggesting that they might be direct NFI-A targets Database analyses of gene function revealed that the changes in gene expression...

Ngày tải lên: 14/08/2014, 07:21

... surrogate for the Xenopus laevis genome, sequence-level analysis can be pursued Microarray analysis of gene expression Microarray analysis of gene expression is the parallelization of the traditional ... to be used for gene expression analysis Changes in global gene expression were measured by comparing the abundance of transcripts using the Affymetrix Xenopus laevis Genome Array GeneChip in Nkx2-5 ... measure gene expression in different tissues, differing stages of the cell cycle, drug dose response, or changes resulting from gene perturbation (knock-out or overexpression) Affymetrix GeneChip...

Ngày tải lên: 24/08/2014, 12:29

... time-course gene expression data analysis 1.4 Challenges in Gene Expression Data-mining Though gene expression data provides the state of a cell by measuring the expres- sion levels of almost all its genes, ... the expression of gene in the KO strain Gene names and the p-values are shown for all genes There is a considerable change in the expression of SIC1 genes between WT and KO strain 58 4.14 Expression ... Clustering of genes into different clusters such that genes within a cluster are more similar in expression is an important challenge in gene expression data analysis This organization of genes into...

Ngày tải lên: 11/09/2015, 09:01

... diabetes alters the expression of genes involved in neuronal migration and neurogenesis in cranial neural tubes of mouse embryos…………… 99 6.1 Expression pattern of developmental control genes is altered ... diabetes altered the expression of genes involved in glycolysis in cranial neural tubes of mouse embryos………………………………………… 95 3.2 Maternal diabetes altered the expression of genes that respond to ... 55 5.8 Data analysis …………………………………………………………… … 57 vi TABLE OF CONTENTS 5.8.1 Absolute data analysis …………………………………………………57 5.8.2 Comparison data analysis ……………………………… ……………58 5.8.3 Gene filtration………………………………………………………….…58...

Ngày tải lên: 11/09/2015, 16:07

... using Cell Quest software (BD) Results Gene expression changes were identified by comparing gene expression profiles across the indicated age groups to the gene expression profiles of the month age ... 100 changed genes Red represents up-regulation of gene expression, and green represents down regulation of gene expression http://www.medsci.org Int J Med Sci 2009, 56 Table Top genes that increased ... 55 Figure Expression profile of the most significantly changed genes with age This profile was generated in the NHGRI website (https://arrayanalysis.ni h.gov), using distance-based analysis of...

Ngày tải lên: 03/11/2012, 11:35

... blotting Semi-quantitative analysis of gene expression profiles, either by Northern blot analysis or by differential display (Section 5), can lead to apparent false expression patterns and so ... possible to interrogate the gene expression patterns in particular cells To investigate which genes are expressed in particular cell types, or which genes are altered in their expression in response ... subsequent Northern blot analysis reveals them to have differential expression patterns (10) 8.6 PCR in a cell: in situ RT-PCR The concept of performing PCR to detect gene expression patterns inside...

Ngày tải lên: 25/10/2013, 22:20

... attributed to a single process in the gene expression cascade Discussion Time-dependent regulation analysis quantifies the relative importance of metabolism and gene expression in FEBS Journal 276 (2009) ... different from other types of analysis because it quantifies the overall importance of metabolism versus gene expression before examining specific metabolites Earlier regulation analysis studies of nitrogen ... to Microsoft excel for analysis using the DDCt method [54] Briefly, cycle threshold (Ct) values were used to calculate the relative level of gene expression of a certain gene (X) normalized to...

Ngày tải lên: 18/02/2014, 06:20

... al (2005) Analysis of long-lived C elegans daf-2 mutants using serial analysis of gene expression Genome Res 15, 603–615 10 Ryu EJ, Angelastro JM & Greene LA (2005) Analysis of gene expression ... serial analysis of gene expression tag sequences into 3¢ complementary DNAs Genes Chromosomes Cancer 33, 252–261 33 van den Berg A, van der Leij J & Poppema S (1999) Serial analysis of gene expression: ... RT-PCR analysis of unknown SAGE tags Nucleic Acids Res 27, e17 34 Chen JJ, Rowley JD & Wang SM (2000) Generation of longer cDNA fragments from serial analysis of gene expression tags for gene identification...

Ngày tải lên: 07/03/2014, 00:20

... Serial analysis of gene expression: rapid RT-PCR analysis of unknown SAGE tags Nucleic Acids Res 27, e17 Chen JJ, Rowley JD & Wang SM (2000) Generation of longer cDNA fragments from serial analysis ... serial analysis of gene expression tags for gene identification Proc Natl Acad Sci USA 97, 349–353 Richards M, Tan SP, Chan WK & Bongso A (2006) Reverse serial analysis of gene expression (SAGE) ... Analysis of human transcriptomes Nat Genet 23, 387–388 Hashimoto S, Suzuki T, Dong HY, Nagai S, Yamazaki N & Matsushima K (1999) Serial analysis of gene expression in human monocyte-derived dendritic...

Ngày tải lên: 07/03/2014, 04:20

... internal control probe in Northern hybridization for normalization of gcGLUT expression Phylogenetic analysis Phylogenetic analysis was performed by maximum parsimony using the PROTPARS program of ... ancestral gene encoding these specific domains In vivo expression and response pattern of gcGLUT to short and long-term hypoxia Fig Phylogenetic analysis of gcGLUT An unrooted tree depicting the phylogenetic ... phylogenetically more similar to GLUT3 than to other class I GLUTs Comparative analysis of the genomic organization of gcGLUT with different human GLUT genes showed that exons 4–9 of the gcGLUT gene, ...

Ngày tải lên: 17/03/2014, 03:20

... regions of these genes The differential expression of CRBPII and I-FABP in the adult zebrafish liver, however, suggests that other transcription factors may regulate CRBPII gene expression in the ... in vitamin A metabolism during zebrafish embryogenesis would be of interest to developmental biologists Moreover, comparative studies of CRBP gene expression in fishes and mammals may provide insight ... Phylogenetic analyses of 51 intracellular lipid-binding proteins, from vertebrates and invertebrates, indicate that at least 14 gene duplications have occurred during the evolution of this multigene...

Ngày tải lên: 17/03/2014, 11:20

... Genomic structure and expression analysis of the Cc RNase Results Expression analysis of Cc RNase In our previous work, we reported the cloning and overexpression of a cDNA sequence of ... the expression profile of the RNase j gene at various developmental stages and in several tissues of the insect C capitata We have also determined the general genomic organization of this gene ... Genomic structure and expression analysis of the Cc RNase ATG TAA PA S1 PA S2 Gene Poly A mRNA 01 AAUAUA mRNA 02 Poly A 100 bp AAUAAA Fig Schematic organization of the Cc RNase gene and its derived...

Ngày tải lên: 23/03/2014, 06:20

... (slc6) gene to ensure that the iCre transgene [10] was correctly expressed (Fig 1A) We generated the transgene by recombination in bacteria, using the tools developed by Lee et al [11] The final ... bars ¼ 50 lm perturbations that could arise from undesired expression of gene products encoded by the BAC transgene, by designing a transgene from which DAT proteins should not be produced To rule ... (Fig 4B) Discussion Transgenesis with small DNA regions regulating transcription is inherently prone to problems of ectopic expression, mosaic expression or the absence of expression, due to the...

Ngày tải lên: 30/03/2014, 08:20

... B gene (from ) 147 bp to + 238 bp).The 529 bp of the promoter region of the cardosin A gene that is relevant for gene expression in Arabidopsis and the corresponding region of the cardosin C gene ... stems, the cardosin A and D genes share a similar pattern of expression, being ubiquitously expressed; (b) cardosin B gene expression is pistil-specific; and (c) cardosin C expression is flowerspecific ... only amplified the corresponding gene, confirming their specificity (B) RT-PCR analysis of cardosin genes, using the corresponding gene- specific primer pairs The actin gene of A thaliana was used as...

Ngày tải lên: 30/03/2014, 09:20

... and diagnostic tools, there is a growing need to utilize readily available peripheral blood for techniques such as SNP analysis, copy number variation analysis and genome-wide gene expression Even ... discrimination between low -expression genes and background, which is indicated by two maxima cDNA amplification significantly reduces the number of genes correlated to globin Genes whose expression increases ... meaningful biological gene expression signature in whole blood, we developed an ex vivo platform for Figure Principal Component Analysis of tissue-specific and globin-related gene expression PCA was...

Ngày tải lên: 18/06/2014, 16:20