oxide protein kinase g iα promotes c src activation proliferation and chemoresistance in ovarian cancer
Tài liệu Báo cáo khoa học: Insulin/protein kinase B signalling pathway upregulates metastasis-related phenotypes and molecules in H7721 human hepatocarcinoma cell line pptx
Ngày tải lên :
21/02/2014, 00:20
... pathway, such as receptor protein tyrosine kinase/ growth factor receptor binding protein 2-son of sevenless protein/ Ras/Raf/mitogen activated protein kinase kinase/mitogen activated protein kinase ... missing links in insulin signal transduction? FEBS Lett 410, 3–10 Dedhar, S., Benjamin, W & Gregory, H (1999) Integrin-linked kinase (ILK): a regulator of integrin and growth-factor signalling ... N-acetylglucosaminyltransferase V (GnT-V) [18,19], which synthesizes a b1,6 N-acetyl-glucosamine (GlcNAc) branch on the asparagine (Asn or N)-linked sugar chains (N-glycans) of glycoproteins...
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Heme oxygenase-1 plays a pro-life role in experimental brain stem death via nitric oxide synthase I/protein kinase G signaling at rostral ventrolateral medulla pps
Ngày tải lên :
10/08/2014, 05:21
... The corresponding sense oligonucleotide: 5′-TCCAG CGGCGTCAGCGGTGC-3′ (ho-1) or 5′-GATCTGACTTCAAG TGATTG-3′ (ho-2) or scrambled oligonucleotide: 5′-CAGTCCCGCGATGGAGCGCC-3′ (ho-1) or 5′-TCAG CAACAATGTCTTCAGA-3′ ... that targets against the coding region (base +10 to -9) of the ho-1 gene [30]: 5′-GGCGCTCCATCGCGGGACTG-3′; or the coding region (base +11 to -9) of the ho-2 gene [30]: 5′-TCTGAAGACATTGTTGCTGA-3′ ... Illustrative gels or summary of fold changes against aCSF controls in ratio of nitric oxide synthase I (NOS I), protein kinase G (PKG), NOS II or nitrotyrosine (NT) relative to b-actin protein detected in...
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Đồ án bước đầu nghiên cứu ép tách protein từ đầu tôm thẻ trong quá trình sản xuất chitin và bổ sung protein thu hồi được vào chượp trong sản xuất nước mắm
Ngày tải lên :
31/08/2014, 06:50
... g3 % & \ G% \ X \Y ? @ > 3 =f = e" e"ff O g 3 y G% & )l iY g= O e"fe g g) ' 2e2 50234 g* ti 7g /OM5gHDg Z9 =C= \* FG* =6* *.03 50_= /0u7 = G % g) { |F 06 \ O = n % o yF z yF z %FG C ... 5% %2 ? C D %7 \ j &\ X;X C o ; Y n , { - \ ;C q %C C D y n - O #B#B## C= =0 5 2c4 IC*0 2C =0>5 +,-* *,e= 7}7# \ \ n "! *):~e=:: O & z ? ' }7 Ơ ;5 ) = " G% C G q% rG% +% G% Ơ ;5 ... } } yF z { e" e"fepe g) |F X G 6X e %7 e" e"ff g= e"fe e) =f g 3 = e" e"ff g) = e" e"ff p g= = e" e"ff g )i ( |F 3 1 %FG 5% Ơ ; % ;G ? ? O n |F 1 ;G %FG %F \ %FG 5% ? 5%7 @ & k% yF...
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Báo cáo khoa học: Malonyl-CoA decarboxylase (MCD) is differentially regulated in subcellular compartments by 5¢AMP-activated protein kinase (AMPK) Studies using H9c2 cells overexpressing MCD and AMPK by adenoviral gene transfer technique potx
Ngày tải lên :
16/03/2014, 16:20
... MCD activity in H 9c2 cells coinfected with Ad.CA-AMPK and Ad.MCD Infection of H 9c2 cells with Ad.MCD resulted in a significant increase in MCD protein and activity when compared with Ad.GFP cells ... that AMPK regulates MCD by increasing levels of mitochondrial MCD protein and activity whereas, cytoplasmic MCD protein levels increased without a change in enzyme activity In vitro incubation of ... expression and activity in cytosolic fractions of H 9c2 cells (A) Western blots for VDAC1 (i), cytochrome C (ii) and MCD expression (iii) in cytoplasmic and mitochondrial fractions from H 9c2 cells infected...
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báo cáo hóa học:" ST6Gal-I expression in ovarian cancer cells promotes an invasive phenotype by altering integrin glycosylation and function" pptx
Ngày tải lên :
20/06/2014, 07:20
... available regarding the role of this enzyme in ovarian cancer The accumulating evidence indicating that ST6Gal-I-mediated integrin sialylation causes increased cell migration and invasion in multiple ... purified integrin receptors enhances receptor binding to collagen I, confirming a critical role for sialylation in regulating integrin function Collagen I has been shown to be secreted in vitro ... diverse cancer types These cancers include: colorectal [4], cervical [5], breast [6], hepatocellular [7], and certain cancers of the head and neck [8] ST6Gal-I is upregulated by oncogenic ras [9-11]...
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báo cáo hóa học:" Aurora kinase inhibitors synergize with paclitaxel to induce apoptosis in ovarian cancer cells" pot
Ngày tải lên :
18/06/2014, 15:20
... Abcam, Cambridge, MA) Staining was scored on a four level scale (0 = no staining, = weak staining, = moderate staining, = intense staining) by a GU pathologist Results Expression Profiling of Ovarian ... staining of TMA core of ovarian carcinoma without adjuvant chemotherapy (20×) (F) Aurora-A staining of TMA core of benign ovarian tissue (20×) (G) Aurora-A staining of TMA core of ovarian carcinoma ... both drugs that function by targeting mitotic cells, but induce apoptosis by different mechanisms Paclitaxel alters microtubule dynamics and induces the spindle checkpoint resulting in mitotic arrest...
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Economic change in the pattani region, c 1880 1930 tin and cattle in the era of siams administrative reforms
Ngày tải lên :
16/09/2015, 17:11
... parallel(s) Concluding Remarks Chapter Tin Mining: Change in the face of European Participation (1890s – 1930s) Prelude Legislation on mining activities: The Mining Act of 1901 Mining land disputes: ... operating in Penang and other parts of the north of Malaya A vertically-integrated industry incorporating smelting, banking, insurance and related financial services, operated by fellow kinsmen and ... following provides a brief overview of the geography and economic significance of the provinces of Pattani (including the district of Saiburi), Yala (including the districts of Bannang Sata and...
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Tài liệu Báo cáo Y học: Enhancement by a-tocopheryl hemisuccinate of nitric oxide production induced by lypopolysaccharide and interferon-c through the upregulation of protein kinase C in rat vascular smooth muscle cells docx
Ngày tải lên :
22/02/2014, 04:20
... incubation Ó FEBS 2002 Tocopheryl succinate-enhanced NO production (Eur J Biochem 269) 2369 Fig Enhancements by TS of LPS/IFN-induced NO production (A) and iNOS induction (B) in VSMC The concentrations ... Lowenstein, C. J (1999) Interaction of interferon regulatory factor-1 and nuclear factor jB during activation of inducible nitric oxide synthase transcription J Mol Biol 289, 459–471 30 Chen, C. -C. , ... Wang, J.-K & Lin, S.-B (1998) Antisense oligonucleotides targeting protein kinase C- a-, -bI, or -d but not -g inhibit lipopolysaccharide-induced nitric oxide synthase expression in RAW 264.7 macrophages:...
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Báo cáo y học: " Protein kinase C promotes restoration of calcium homeostasis to platelet activating factor-stimulated human neutrophils by inhibition of phospholipase C" ppt
Ngày tải lên :
11/08/2014, 08:22
... delayed clearance of cytosolic calcium Br J Pharmacol 2002, 136:81-9 Tintinger GR, Theron AJ, Steel HC, Anderson R: Accelerated calcium influx and hyperactivation of neutrophils in chronic granulomatous ... Chemicals and reagents The highly selective protein kinase C inhibitor, GF10903X, was purchased from Tocris Cookson Ltd, UK Unless indicated all other chemicals and reagents were obtained from the Sigma ... systems (Figure 1) Activation of neutrophils with FMLP resulted in an abrupt increase in fura-2 fluorescence intensity which coincided with the rise in cytosolic Ca2+ concentrations, and quickly (.714'5%'0%'...
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Tài liệu Báo cáo khoa học: Phosphorylation of hormone-sensitive lipase by protein kinase A in vitro promotes an increase in its hydrophobic surface area ppt
Ngày tải lên :
18/02/2014, 11:20
... product obtained using the sense primer 5¢-ATC ATC TCC ATC GAC TAC TCC CTG-3¢, the antisense primer 5¢-AAG AAT TCT AGA TTA ATG GTG ATG ATG GTG ATG ATG GTG TGG GGT CAG CGG TGC AGC AGG GGG GGT-3¢ ... adipocyte HSL To generate a recombinant baculovirus encoding C- terminally tagged rat adipocyte HSL, full-length rat adipocyte HSL cDNA, including a sequence encoding one Pro residue and eight C- terminal ... phosphorylation induces a conformational change that increases the accessible hydrophobic surface area, enabling freer access to the lipid-binding site, in return for weaker binding This is well in line...
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Tài liệu Báo cáo khoa học: Protein kinase CK2 activates the atypical Rio1p kinase and promotes its cell-cycle phase-dependent degradation in yeast pdf
Ngày tải lên :
18/02/2014, 16:20
... a nucleotide sequence encoding an HA3-tag, both Fig Rio1 protein kinase (A) Domains of the Rio1p kinase are indicated (according to the classification of Hanks et al [45]); candidates for CK2 ... and pepstatin, twice in kinase buffer, and used for in vitro kinase assays In vitro kinase assays Recombinant human protein kinase CK2 holoenzyme (0.5 U, corresponding to ng; New England Biolabs, ... yeast cells Biochem Biophys Res Commun 346, 786–793 11 Hanna DE, Rethinaswamy A & Glover CVC (1995) Casein kinase II is required for cell cycle progression during G1 and G2 ⁄ M in Saccharomyces cerevisiae...
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Tài liệu Báo cáo khoa học: The diacylglycerol and protein kinase C pathways are not involved in insulin signalling in primary rat hepatocytes doc
Ngày tải lên :
20/02/2014, 02:21
... e) protein kinase C isoforms on insulin-stimulated translocation of epitope-tagged GLUT4 glucose transporters in rat adipocytes: speci c interchangeable effects of protein kinases C- Z and C- 1 ... propagation of hormone and growth factor signals The recent finding that focal adhesion kinase regulates protein kinase B, glycogen synthase kinase- 3 and glycogen synthase, in an insulindependent manner ... (positive control for PKC translocation), a second bolus of 50 pM glucagon or a staggered infusion of 10 nM insulin (25–35 min) and 50 pM glucagon (Fig 7B ,C) The antiglucagon effect of insulin was...
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Tài liệu Protein chức năng: Collagen – Gelatin – Glucoprotein, những loại thực phẩm chức năng duy trì sức khỏe, sự trẻ trung doc
Ngày tải lên :
25/02/2014, 21:20
... Nươ c uống chư c tăng c ờng c ́u tru c collagen Nư c uống Collagen chăm s c s c đẹp cho da Khi thể bư c qua tuổi 30, t c độ tổng hợp collagen bị chậm lại, t c độ phân hủy collagen gia tăng Trung ... Hyaluronic glycosaminoglycan (GAG), mà chất g n collagen elastin để tạo thành sụn Acid Hyaluronic không giúp cho sụn đệm khớp mạnh mẽ linh hoạt, mà giúp tăng nguồn cung c p chất lỏng hoạt dịch liên ... Gelatin c ng c mái t c, giữ cho to c sáng bóng khỏe mạnh • Gelatin đươ c thể sử dụng để tái tạo móng tay, móng chân • Gelatin còn giúp tạo nên dàn giá cho bộ xương Gelatin có chứa...
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Báo cáo khoa học: Identification of the structural determinant responsible for the phosphorylation of G-protein activated potassium channel 1 by cAMP-dependent protein kinase pdf
Ngày tải lên :
07/03/2014, 00:20
... (PKA-cs) and [32P]ATP[cP] Autoradiograms of subsequent SDS gels revealed GIRK1 migrating in two bands, indicating a glycosylated and a nonglycosylated form, as reported previously [24] Interestingly, ... HK+ acetylcholine, HK and back to ND96 Cytosolic injection of cAMP and cAMP analogs (30–60 pmol per oocyte) was performed as described previously [18] The current increase, following cAMP injection, ... signalling complex comprising (amongst other signalling molecules) GIRK1, GIRK4, Gb ⁄ c, PKA, PP2A and protein phosphatase was identified to exist in rat atrial membranes in situ [21], supporting...
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Báo cáo khoa học: The 3¢-UTR of the mRNA coding for the major protein kinase C substrate MARCKS contains a novel CU-rich element interacting with the mRNA stabilizing factors HuD and HuR ppt
Ngày tải lên :
08/03/2014, 08:20
... TTT CTT TCT TTC TTT TTT TTT TTC TCG AGC CCC-3¢; antisense: 5¢-GGG GCT CGA GAA AAA AAA AAA GAA AGA AAG AAA GAA AGA AAG AAA GAA AGA AAG AAA GAA AGG AAT TCG GGC CCG GGG-3¢) representing base pairs ... pBS-MARCKS 52 nt CU-element plasmid (pBSMARCKS 52 nt) was constructed by annealing the two synthetic oligonucleotides (sense: 5¢-CCC CGG GCC CGA ATT CCT TTC TTT CTT TCT TTC TTT CTT TCT TTC TTT CTT ... amplified a cDNA encoding residues 2–373 of HuD by PCR using clone kuniZAP-265114 as template and BamHI and SmaI sites containing primers (sense: 5¢-TAG CGG ATC CGA GCC TCA GGT GTC AAA TGG-3¢; antisense:...
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Báo cáo khóa học: Phospholipase C, protein kinase C, Ca 2+ /calmodulin-dependent protein kinase II, and redox state are involved in epigallocatechin gallate-induced phospholipase D activation in human astroglioma cells ppt
Ngày tải lên :
16/03/2014, 16:20
... 3D), suggesting that PLC -c1 is involved in this process EGCG induces a rise in [Ca2+]i in U87 cells As EGCG stimulates PLC activity, it might induce an increase in [Ca2+]i in U87 cells [Ca2+]i ... of PLC -c1 in the signaling pathway Expression of the inactive mutant of PLC -c1 also attenuated EGCG-induced PLC activation In resting cells, PLC -c1 is located predominantly in the cytosol, and ... cells Involvement of PKC in EGCG-induced PLD activation Discussion Phosphoinositide-speci c PLC activation by EGCG leads to the production of two second messengers, Ins(1,4,5)P3 and diacylglycerol,...
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Báo cáo khoa học: Endogenous mono-ADP-ribosylation mediates smooth muscle cell proliferation and migration via protein kinase N-dependent induction of c-fos expression potx
Ngày tải lên :
17/03/2014, 09:20
... AGATGGCTGCAGCCAAGTGC-3¢, antisense: 5¢-AAG GAAGACGTGTAAGCAGTGCAGC-3¢), and c- myc (sense: 5¢-AAGTTGGACAGTGGCAGGGT-3¢, antisense: 5¢-TTGCTCCTCTGCTTGGACAG-3¢) Amplification was conducted over 35 cycles using a ... the GeneAmp kit with oligodeoxynucleotide primers speci c for GAPDH (sense: 5¢-CGGTGTG AACGGATTTGGCCGTAT-3¢, antisense: 5¢-AGCCTTC TCCATGGTGGTGAAGAC-3¢); c- fos (sense: 5¢-GAATA AGATGGCTGCAGCCAAGTGC-3¢, ... Biochem 270) include various GTP-binding proteins, including Gs, Gi and Rho [13–15] Although these target proteins suggest a role for mono-ADP-ribosylation in signal transduction, the functional...
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