oligonucleotide induced modification of alternative splicing in cancer

... length All introns Proportion of bases 50% All intron Proportion of introns (b) Repartition of bases within the codons Insertion of introns within the CDS Number of introns Proportion of introns ... (36) Introns were detected in 5' UTRs The number of 5' UTR introns or of genes containing 5' UTR introns is indicated in parentheses motifs (Figure S2a,b in Additional file 2) This finding was ... also detected in the BP of 3n + introns in frame 0, 3n + introns in frame or 3n introns in frame if the S2 distance is indeed bp (c) The main 3'ss is CAG, but, in about 10.5% of the introns, TAG...

Ngày tải lên: 09/08/2014, 20:22

... AS Of all the isoforms encoded by the remaining genes, 31,618 are classified as non-protein coding according to the Ensembl annotations In the remaining genes, we found 7,467 isoforms differing ... continuously being produced opens the way to the investigation of relevant properties of living organisms and can be effectively exploited in many instances Cataloguing all putative isoforms of ... domains are more often truncated in unknown isoforms than in positive ones We verified whether AS would remove existing annotated active sites present in other isoforms of the same gene in the...

Ngày tải lên: 09/08/2014, 22:23

... located in the coding region of these transcripts, the resulting protein isoforms will in many cases differ by an indel (only these type of sequence variations were considered in this study) In order ... Giorgetti A, et al: The implications of alternative splicing in the ENCODE protein complement Proceedings of the National Academy of Sciences of the United States of America 2007, 104(13):5495-5500 ... the an initial peptide population This initial peptide population consisted of all peptides that theoretically could be obtained through digestion of the proteins (including isoforms resulting from...

Ngày tải lên: 11/08/2014, 11:20

... Insulin regulates alternative splicing of protein kinase C beta II through a phosphatidylinositol 3-kinasedependent pathway involving the nuclear serine/argininerich splicing factor, SRp40, in ... changes in alternatively spliced mRNA isoforms induced by insulin and wingless Figure Numerous changes in alternatively spliced mRNA isoforms induced by insulin and wingless (a) Features of microarray ... categories of differentially spliced genes upon activation of the wingless pathway (Table 1) and changes in alternative splicing of several key genes involved in wingless signaling, including the wingless...

Ngày tải lên: 14/08/2014, 21:20

... Developmental profile of editing in ADAR2 substrates in mouse brain Figure 3.3 Spatial profile of editing in ion channels in adult mouse Figure 3.4 Spatial profile of editing in ADAR2 substrates in adult ... 1.4 Alternative splicing diversifies the function of calcium channels 1.4.1 Mechanism of alternative splicing 1.4.2 Effects of alternative splicing in L-type calcium channels 1.4.3 CaV1.3 in the ... IQ motif in mouse lumbar and whole brain Figure 2.6 Profile of editing in CaV1.3 IQ motif in different mouse brain regions Figure 2.7 Developmental profile of editing in CaV1.3 IQ motif in mouse...

Ngày tải lên: 10/09/2015, 15:48

... and eosin staining We examined the expression of these proteins in a cancer- free layer of squamous epithelial cells, carcinoma in situ (CIS) and invasive cancer, all of which were observed in the ... comparing the amounts of Mcm in total cellular proteins and chromatin-bound proteins, approximately one-third of total Mcm protein was found to be recovered in the chromatin-bound fraction in HeLa ... cellular proteins but also in chromatin-bound proteins The Mcm proteins bound to chromatin probably function in DNA replication, but the role of the proteins not tightly bound to chromatin remains to...

Ngày tải lên: 20/02/2014, 23:20

... molecules, integrins and interacting with phosphorylation sites for serine ⁄ threonine and tyrosine kinases, thus contributing to cancer development [11] Roles of MMPs in cancer progression During development ... tetracyclines, lacking antibiotic activities, may inhibit MMPs by binding to metal ions such as zinc and calcium This family of inhibitors, including metastat (COL-3), minocycline and doxycycline, cause ... membrane-anchored ligands of EGFR (HB-EGF, TGF-a and amphiregulin) Shedding of E-cadherin Activation of TGF-b Shedding of HB-EGF Proliferation Cleavage of Fas ligand Shedding of tumor associated major...

Ngày tải lên: 15/03/2014, 00:20

... issue of vaccinations reveals a more complex picture—both in terms of differences of opinion regarding the nature of the practice, and in terms of different ways of positioning vaccination in the ... radical fringes of alternative medicine Such a tendency is in fashion not only in current medical publications, but also in writings within the realm of the history of medicine.5 Yet as of late ... compelled to vaccinate by demands of Boards of Health I administer one of the potencies of Variolin or Vaccinin…and scratch the same into the arm, certifying to the fact of vaccination I not urge...

Ngày tải lên: 15/03/2014, 09:20

... on the kinetics of the CoQ-oxidizing module (C) and CoQ-reducing module (D) The kinetics of the CoQ-oxidizing module were obtained by titrating with a specific inhibitor of the CoQ-reducing module, ... Effect of Cd2+ and Cu2+ ions on the kinetics of the CoQ-reducing and CoQoxidizing modules Effect of Cd2+ on the kinetics of the CoQ-oxidizing module (A) and CoQ-reducing module (B) Effect of Cu2+ ... this is often limited by the feasibility of assigning modules that interact via a single connecting intermediate [28] In this study, we used modular kinetic analysis and MCA to determine the...

Ngày tải lên: 16/03/2014, 02:20

... factor) Interleukin 1, beta Mitogen-activated protein kinase 14 Mitogen-activated protein kinase 13 Mitogen-activated protein kinase Mitogen-activated protein kinase Mitogen-activated protein kinase ... Mitogen-activated protein kinase Rhodopsin (opsin 2, rod pigment) (retinitis pigmentosa 4, autosomal dominant) Rho-associated, coiled-coil containing protein kinase Ras-related C3 botulinum toxin substrate ... enzymes that in uence transcription by selectively deacetylating or acetylating the e-amino groups of lysine located near the amino termini of core histone proteins Acetylation of p65 at lysines 218,...

Ngày tải lên: 22/03/2014, 16:20

... preliminary feasibility assessment of using alternative fuels in China This report provides an overview of the technical and qualitative characteristics of a wide range of alternative fuels including ... necessary next step in assessing the viability, costs and benefits of using ASR in cement kilns in China b Textiles According to Chinese government statistics, the textile industry in China produces ... landfilling and other forms of thermal combustion In comparison to other incineration processes for energy capture, end use in cement manufacturing has the key benefits of utilizing pre-existing infrastructure...

Ngày tải lên: 24/03/2014, 05:20

... secondary structures are involved in regulated pre-mRNA splicing is central to the deciphering of the splicing code towards predicting splicing outcomes A splicing silencer element in HBV-PRE HBV-PRE ... expression of the PTB protein Figures 4A and 1D clearly show that the absence of the PTB protein did not affect the silencer activity of PRE-ISS, arguing against a role of PTB binding in this splicing ... elements in the neighboring introns of SMN exon repress exon inclusion in its SMN2, but not SMN1, splicing context [23,27,28], suggesting that the exon inclusion mechanism in the SMN1 splicing context...

Ngày tải lên: 28/03/2014, 23:20

... unsettling reminder to me, as a medical historian, that the long record of interprofessional warfare continues to strain interactions between the two sides Time is in the process of relieving the ... condemnation of any If I am taking a position, it is simply that of the first director of the Of ce of Alternative Medicine, speaking “not as an advocate of alternative medicine, but as an advocate for ... founding of the OAM, followed by Eisenberg’s study (1993), the opening of the first publicly funded natural medicine clinic in the country (King County, Washington, 1996), and other revelations of...

Ngày tải lên: 29/03/2014, 07:20

... by institutional structures, policy initiatives, or clinical concerns? Developments in cancer research in the 1990s, including rapid advances in understanding of cancer genetics and increasing ... in each set of specialty journals Here, instead of selecting and mapping some portion of the most specific links, we mapped only the links between the nearest nodes, as follows The strength of ... translational interface defined by specific approaches (e.g., gene expression profiling) that are used across all cancer types In cardiovascular medicine, in contrast, there appear to be distinct clinical...

Ngày tải lên: 18/06/2014, 19:20

... by institutional structures, policy initiatives, or clinical concerns? Developments in cancer research in the 1990s, including rapid advances in understanding of cancer genetics and increasing ... in each set of specialty journals Here, instead of selecting and mapping some portion of the most specific links, we mapped only the links between the nearest nodes, as follows The strength of ... translational interface defined by specific approaches (e.g., gene expression profiling) that are used across all cancer types In cardiovascular medicine, in contrast, there appear to be distinct clinical...

Ngày tải lên: 20/06/2014, 03:20

... history including past cancer history, b) physical examination, c) recording of medications and recent therapies, including analgesic consumption, d) pain assessment using the Brief Pian Inventory ... average pain score of ≥ points (pain intensity difference, PID, >/= 2) at week (day 28 from inclusion) over baseline As to the assessment of the quality of analgesic care, the adequacy of pain management ... reporting on the results of the "epidemiological" part of the study, including a description of patients' characteristics in terms of baseline socio-demographic and clinical variables, pattern of...

Ngày tải lên: 20/06/2014, 15:20

... PET-FDG imaging modified the management of 13.7% of patients Our findings indicate that PET-CT changed the stage in a large number of patients: upstaging approximately 43.4% and down staging 5.7% ... carcinoma [21] Additionally, our study highlights the importance and usefulness of pre- treatment PET-CT in detecting RPLN status and its role in guiding definitive treatment in OPSCC Our findings ... disease The introduction of PET-CT imaging for assessment of cancers of the head and neck revolutionized cancer staging and is now routinely performed prior to planning therapy However, few studies...

Ngày tải lên: 09/08/2014, 03:22

... successful detection of experimentally induced intron retention in a number of genes containing introns Yeakley et al [22] detected alternative splicing in six human genes using a fiber-optic microarray ... bring to the study of alternative splicing, but they also show the challenges of the task It is significant that both studies [7-9] addressed only one kind of alternative splicing: monitoring individual ... about half of these represented genuine alternative- splicing events This work [18] clearly demonstrates that microarrays can detect alternative splicing, but many types of alternative splicing have...

Ngày tải lên: 09/08/2014, 20:20

... any mixed gain-loss pairs We also detected the trisomy of 1q [21], which appeared in all tumor categories in the associations involving gain of chromosome (Figure 2a; Figure S2a-d in Additional ... involved only in expected links (for example, a link between a translocation and a gain/loss of one of its derivative chromosomes; a link between two (two-break) translocations originating from one ... ‘aberration calling’), and handling intersecting aberrations (for example, two deletions with overlapping segments) Another major difficulty in uncovering strong associations in cancer data is...

Ngày tải lên: 09/08/2014, 23:20

... development of resistance to these agents The results of ongoing clinical trials with mTOR inhibitors, as single agents and in combination, will better define their activity in cancer Competing interests ... plays in cellular signaling suggests a broad range of clinical utility, and indeed, phase I clinical evaluations of all mTOR inhibitors provided preliminary evidence of anticancer activity in multiple ... S6K, to initiate ribosomal translation of mRNA into protein mTOR activation leads to increased synthesis of multiple proteins, including several that have been implicated in the pathogenesis of multiple...

Ngày tải lên: 10/08/2014, 22:20