of muscle mirnas in cell lines

... isolated and individually grown as cell lines, the concentration of neomycin was decreased to 500 μg/ml The individual cell lines showed some variability in growth rate To check expression of various ... self-replication of engineered minigenomes in cell cultures, has been established for HCV replication in other regions of the world [7,37] The HCV stable cell lines may be very useful in the study of HCV ... this study was initiated to establish stable cell lines expressing proteins of Pakistani HCV genotype 3a isolates The establishment of HCV genotype 3a cell lines stably expressing structural...

Ngày tải lên: 14/08/2014, 19:22

... infectivity of RGNNV against the medaka cell lines (Table 1) by detecting CP-expressing cells at day post-inoculation (dpi) When the cells were inoculated with RGNNV having the 50% tissue culture infectious ... be restricted in some of the medaka cell lines though the Table 1: Medaka cell lines used in this study Cell line Species Strain Tissue derived OLHNI-1 Oryzias latipes HNI Caudal fin OLHNI-2 O ... Unknown Fin OLME-104 O latipes HB32C Melanoma Figure Infectivity of RGNNV in various medaka cell lines Each cell line (1.0-1.5 × 105 cells) was inoculated with 105 or 106 TCID50 of RGNNV and incubated...

Ngày tải lên: 12/08/2014, 04:20

... grouped into trafficking related clusters such as integrins, Mmps, chemokine and chemokine receptors, and interleukins and interleukin receptors Integrins are key mediators of cell- cell interactions, ... Il18r1 interleukin alpha [NM_010554] interleukin [NM_031168] interleukin 18 [NM_008360] interleukin 17D [NM_145837] interleukin beta [NM_008361] interleukin 11 receptor, alpha chain [NM_010549] interleukin ... paid to genes involved in cell trafficking, namely integrins, metallopeptidases, chemokines and chemokine receptors, as well as interleukins and interleukin receptors (Table 1) In order to visualize...

Ngày tải lên: 12/08/2014, 14:20

... enhanced/complemented by reintroduction of full length INI1 into these cells Interestingly this enhancement was not efficient for virions deleted of IN indicating that perhaps, binding of IN to INI1 is required ... 0.024 for MON) in the intracellular levels of p24 in both MON and 293T cells in the presence of INI1 (Figure 3A) However, the presence of INI1 in MON cells resulted in further increase (about ... another host protein unrelated to INI1 in MON cells It remains to be determined as to what causes the block in incorporation of INI1 into HIV-1 particles in MON cells It is interesting to note that...

Ngày tải lên: 13/08/2014, 09:20

... assumptions behind our model of oligodendrocyte development in cell culture In what follows, we describe our experimental findings in the context of the model presented in Section Each element of the ... mammalian cells [28] In that case, the incomplete separation seems to be a consequence of failure of chromatin segregation As our cells are cultured in serum-free defined medium in the absence of any ... O-2A/OPCs increases in the presence of thyroid hormone The probability of differentiation increases from 0.07 in Generation to 0.21 in Generation in the absence of thyroid hormone and from 0.18 in Generation...

Ngày tải lên: 13/08/2014, 23:20

... optimal size remains at Ω≈103 μm3 for a wide range of signal amplitudes and control parameters, which is of the same order of real living cells in vivo Since the internal noise in living cell systems ... may often receive weak signals, our findings may have quite significant implications for living cell systems and may imply the ubiquitous importance of internal noise in functioning processes in ... that, near the Hopf bifurcation point, instead of trying to resist the internal molecular noise, living cell systems may have learned to exploit the internal noise to intensively enhance the ability...

Ngày tải lên: 02/09/2015, 13:20

... area of great interest, as loss of genomic integrity is often implicated in tumorigenic and oncogeneic events Critical to the ability of healthy cells in maintaining genomic integrity are the cell ... Induction in Hela cells in response to Adriamycin and varying doses of p38 inhibitor Figure 5.7: Inhibition of p38 induces apoptosis in A549 cells Figure 5.8: Apoptosis induction by MMS and LY479754 in ... associated proteins including Nlp (ninein-like protein), Katanin and the microtubule stabilizing protein TCTP (47,296) Besides being involved in the active recruitment of key components of the microtubule...

Ngày tải lên: 11/09/2015, 09:02

... chromatin resulting in either gene activation or repression For instance, the appearance of histone acetyltransferases, including cAMP-responsive element binding (CREB) protein-binding protein (CBP), ... 2000) CDK protein levels remain stable during the cell cycle, in sharp contrast to their activating proteins, the cyclins The oscillatory expression of most cyclin proteins during cell cycle progression ... the cyclin-dependent kinases (CDKs), a family of serine/threonine protein kinases that are activated at specific points of the cell cycle To date, nine CDKs have been identified and, of these,...

Ngày tải lên: 13/09/2015, 20:18

... as cell attachment, may in uence the expression of HO-1 The dominant expression of HO-2 protein, in comparison with the low expression of HO-1 protein, in H146 small cell lung cancer cells is of ... maintained in HO-2– ⁄ – mice through appropriate resetting of HO-1 expression In contrast with HO-1 expression, expression of HO-2 mRNA and protein was not increased in several human cell lines ... undetectable In contrast, HO-2 mRNA and protein were both expressed in all eight cell lines (Fig 1A,B) We also measured the heme content of these cell lines: it was about twofold higher in YN-1 and...

Ngày tải lên: 19/02/2014, 05:20

... Effects of hypoxia on HO-1 and HO-2 expression in human cell lines We initially analyzed the effects of hypoxia on the expression of HO-1 and HO-2 in human cell lines of Reduced expression of heme ... expression levels of HO-2 mRNA and protein in five out of six cell lines, with the exception of KG1 myeloid cells A Hypoxia decreases the expression levels of HO-2 protein in YN-1 and K562 cells To confirm ... levels of HO-2 mRNA and protein in five out of six human cell lines: Jurkat, YN-1, K562, HeLa and HepG2 In this context, Newby et al [29] described decreased levels of HO-2 protein in placentas of...

Ngày tải lên: 19/02/2014, 06:20

... provide a possible model of drug interaction in GEO cells, which involves the complex interaction of proteins involved in cell cycle regulation To recapitulate, combining CDO with cytotoxic chemotherapy ... alone did not increase the extent of staining However, in cells that had been cocultured with CDO and a cytotoxic drug, staining was significantly increased, indicating the presence of senescence ... reduction in Fig The effect of CDO in HCT116 and GEO cell lines on day The activity of CDO in the sensitive cell lines was fitted a standard Emax model Representative DNA histograms following culture...

Ngày tải lên: 07/03/2014, 15:20

... protease, legumain, because of removal of the key amino acid in the legumain-binding site of cystatin C [22] The amino acid substitutions reside in opposing parts of the cystatin C molecule, which ... the cysteine inhibitory capacity of cells The human cancer cell lines chosen included one epidermoid carcinoma cell line, three mammary tumour cell lines and one human pancreas adenocarcinoma All ... an increase in some of the cell lines could be detected and after 30 the cell fluorescence had increased in all five strains The pattern of internalization was more or less equal in the five strains...

Ngày tải lên: 16/03/2014, 06:20

... shows the binding of labelled oligomers to the nuclear-enriched fraction of proteins The efficiency of labelling of the single oligomers being not homogenous, the ratios of the different binding signals ... amount of recovered protein owing to the presence of a high molar excess of BSA remaining in the recovery buffer Therefore, a fixed aliquot of the protein was incubated with ng of the indicated ... oligomers showing irrelevant differences in growth inhibition The overall results suggest that the kinetics of binding of GT-G2 to eEF1A might be slower with respect to that of GT-G1 explaining its...

Ngày tải lên: 16/03/2014, 13:20

... fluorescence measured in q0 cells was lower than that in the parental cell lines containing mtDNA The decrease was substantial in lung (90%) and muscle (40%) cells but was less evident in bone (less ... eliminate intracellular oxidants under standard growth conditions Yet, in the absence of glucose (stress conditions), intracellular levels of ROS in bone-derived q0 cells are 2.5 times those of muscle ... suggest that increased generation of intracellular ROS in bone q0 cells, relative to muscle q0, is due to increased production of oxidants The high production of ROS in bone-derived q0 cells is further...

Ngày tải lên: 16/03/2014, 18:20

... of glycosphingolipids occurring at high density in rafts All these properties may be characteristic of human T cell lines of leukemia/lymphoma origin, as a recent study in mouse cell lines [52] ... T lymphoma/leukemia cell lines, on cell- by -cell basis, using flow cytometry The cell- independent intramolecular FRET between light and heavy chains of class I HLA (used as Ôinternal standardÕ) ... raft-association of the IL-2Rb chain in transformed human NK and fibroblast cells Thus, to better understand the role of lipid rafts in cell growth/ viability-signaling of T cells, in general, and in the...

Ngày tải lên: 17/03/2014, 23:20

... viability was measured at intervals of 24 h (B) Cell viability of HEL cells (C) Cell viability of HEp-2 cells (D) Cell viability of PMNs PMNs maintained about 80% viability in control cultures after 48 ... (h) D 100 80 HEL cells 120 72 HEp-2 cells 120 Cell viability (% of control) 100 C Cell viability (% of control) B HaCaTcells 120 Cell viability (% of control) Cell viability (% of control) A 72 ... cell lines, such as human keratinocytes (HaCaT cells), human embryo lung fibroblast cells (HEL cells), human nasopharyngeal carcinoma cells (HEp-2 cells) and PMNs in vitro Results O2–-scavenging...

Ngày tải lên: 23/03/2014, 07:20

... variety of human cell lines; primate and canine cell lines may also be used [5] It has been extensively documented that the plasmids are maintained episomally (5–10 copies per cell, e.g for 293 cells), ... avoided In summary, we have developed plasmid pEBTetD for full establishment of doxycycline-inducible protein expression in human cell lines by just a single transfection For closely matching cellular ... results for pEBTetD cell pools in continuous culture up to 10 weeks indicate useful factors of inducibility on the level of protein function Clearly, in many situations, e.g in RNA interference experiments,...

Ngày tải lên: 23/03/2014, 09:21

... other MMPs in at least one of the cell lines For MMP-8, -12 and -21 only very weak mRNA expression could be observed in single cell lines Nine MMPs, which were present in most of the cell lines, were ... summarized in Additional file 1: MMP expression in gynecological cancer cell lines Methods Cell culture All the cell lines used were described in Table [20-45] and obtained from Cell Lines Service ... pattern concerning endometrial cancer cell lines In addition, MMP-3, -10 and -13 were expressed in cervical carcinoma and teratocarcinoma cell lines only Caski and PA-1 cell lines could be the...

Ngày tải lên: 28/03/2014, 14:20

... protein was more abundant in some cell lines, such as C4-I, HeLa, MCF7 or the colon carcinoma WiDr, and detected in smaller amounts in the remaining carcinoma cell lines (Fig 3A) In lymphoma cell ... is detected in the nucleus in some cell lines and in transfected cells [11,13], but it is also present in the cytosol in other cell lines [15], particularly in some types of adenocarcinoma (unpublished ... expression of VRK2A and VRK2B proteins in tumour cell lines To demonstrate that the two messages identified code for real proteins, we determined their presence in a panel of tumour cell lines using...

Ngày tải lên: 30/03/2014, 11:20

... effect of dasatinib in combination with chemotherapy was examined in the three dasatinib responsive cell lines, Lox-IMVI, HT144 and Malme-3M and in one of the dasatinib-resistant cell lines, Sk-Mel-28 ... dasatinib Discussion We have evaluated the effects of dasatinib, a multi-targeted tyrosine kinase inhibitor, in human melanoma cell lines [6] In a previous study in breast cancer cell lines, ... evaluation of dasatinib in clinical trials in melanoma patients Two clinical trials of dasatinib in melanoma are currently underway, including a phase I/II study of dasatinib in combination with...

Ngày tải lên: 18/06/2014, 15:20