nutrient kinetics of benthic algae

... production of planktonic algae will be exported further and faster in lotic ecosystems than excess production of benthic algae Thus, the im- pact of Nversus P-loading on the structure and func- tion of ... influence of natural substrate type on benthic algae assemblages These results contrast with a review on the use of artificial substrates for benthic algal studies which suggests that choice of material ... study of lakes of differing trophic status, Ellis et al (2001) found that the nature of the substrate (glass, wood, plastic) considerably affected the patterns of colonisation of periphytic algae...

Ngày tải lên: 28/07/2014, 17:21

... characteristics of [125I]TC-PCSK9 binding to HepG2 cells To determine whether the kinetics of binding of PCSK9 to HepG2 cells can be described as a simple bimolecular reaction, the kinetics of [125I]TC-PCSK9 ... [koff] of 0.0009 ± 0.00012 min)1 (n = 3), which corresponds to a half-time of dissociation of 770 Lowering the pH of dissociation medium to 6.2 also led to monophasic and slow dissociation of ... Mousavi et al Characterization of the binding of PCSK9 to intact cells levels of plasma LDL cholesterol, whereas loss -of- function mutations are associated with low levels of plasma LDL cholesterol...

Ngày tải lên: 14/02/2014, 14:20

... the presence of increasing DP may be a common feature of glucansucrase Additional studies are required to elucidate the dextran-dependent synthesis of glucan As the affinity of the GBd of GTF-I for ... in the absence of dextran between GS and GSGB In addition, the sucrose hydrolysis activities of GS and GSGB as a function of sucrose concentration obeyed simple Michaelis–Menten kinetics, with ... previous report [14] Time course of dextran-independent glucan synthesis We examined the kinetics of insoluble glucan synthesis from sucrose by GS and GSGB in the absence of dextran (Fig 3A,B) For this...

Ngày tải lên: 14/02/2014, 22:20

... methodology to the study of the kinetics of catalysis by the serine protease a-CT we were able to statistically correlate the structurally dynamic behavior of the enzyme with its kinetics of catalysis From ... cutoff distance (rc ẳ 6.0 A) The dynamics of the resulting network are then dened by the Ni ã Nj Kirchhoff connectivity matrix of interresidue contacts (G) where the off diagonal elements of ... and protonation rates of Ser195 thus reducing the kinetics of catalysis The results also suggest that the dynamics of the calcium binding site in this structural class of proteins (chymotrypsin-fold...

Ngày tải lên: 19/02/2014, 05:20

... oligomeric forms of the enzyme will not interfere with the interpretation of the data In this research, we have analyzed the steady state kinetics of the glutaminase reaction of CTP synthase from ... time are derived from the heat of dilution of glutamine into the reaction cell (B) The heat generated by hydrolysis of 0.15 lM of glutamine used to determine DH of the reaction ể FEBS 2002 4774 ... GTP activation of the glutaminase activity in the presence of 0.1 mM (open circles) or mM (closed circles) each of UTP and ATP-cS (C) GTP activation of CTP synthesis at 0.1 mM each of UTP and ATP...

Ngày tải lên: 21/02/2014, 15:20

... concentrations of the non-physiological reductant dithiothreitol These data suggest that the DS-dependent inhibition of NAT1 is unlikely to be a result of the formation of a mixed disulfide, but rather of ... constant for the inhibition of NAT1 by DS (ki) was deduced from the slope of kobs as a function of the DS concentration [DS], and was · 104 m)1Æmin)1 The order of the reaction of DS with NAT1 (n) can ... identification and characterization of the molecular targets of DS are of prime importance in order to understand the pharmacological and ⁄ or toxicological effects of this therapeutic compound In...

Ngày tải lên: 07/03/2014, 02:20

... phosphinic acid derivatives of GSH as moderate Kinetics and inhibition of ATP-dependent ligases inhibitors of CfGspS [38] The most active of these was a phosphonic analogue of GSH (c-l-Glu-l-Leu-GlyP), ... Results Initial velocity analysis of the kinetic mechanism of GspS A matrix of kinetic data was collected in order to determine the kinetic mechanism of GspS Six families of kinetic data were generated ... the concentration of phosphinate and initiating with enzyme Modality of inhibition The mode of inhibition of the slow-binding phosphinate was determined by examining the effect of varying each...

Ngày tải lên: 07/03/2014, 04:20

... mutagenesis of Ser218 to Ala, as similar analysis of the mutated protein showed no evidence of phosphorylation [26] Residue S218 is detected by a variety of phosphorylation prediction software to ... Kd of SEPT2 is 0.28 lm and that of GDP is 1.7 lm in the presence of physiological Mg2+ concentrations These values are about an order of magnitude lower than those observed for complexes of yeast ... completion of most of the studies described herein Given the conserved nature of this residue we therefore mutated the residue back to arginine and found that none of the properties of the protein...

Ngày tải lên: 07/03/2014, 12:20

... elan-immunoreactive material Fig Evolution of rec-trappin-2 production by Pichia pastoris as a function of the duration of fermentation Aliquots of concentrated supernatants of rec-trappin-2-secreting P pastoris ... Simon, S.R (2001) Kinetics of the Inhibition of Proteinase by Elan Am J Respir Cell Mol Biol 24, 8389 25 Bieth, J.G (1995) Theoretical and practical aspects of proteinase inhibition kinetics Methods ... before measurement of the residual enzymatic activity by addition of 10 lL of a 100 mM Suc(Ala)3-p-NA stock solution Product release was continuously recorded until a constant rate of paranitroaniline...

Ngày tải lên: 07/03/2014, 15:20

... reactive ester of NIPGB in kinetic experiments, failed because of the high rates of spontaneous hydrolysis of this compound The only substrate of PA for which the steady-state rate of hydrolysis ... analysis of penicillin acylase (Eur J Biochem 270) 3681 Fig Inhibition of NIPGB hydrolysis by 7-ADCA Lineweaver–Burk plot of the effect of various concentrations of 7-ADCA on the conversion of NIPGB ... concentration [N] and the results of the inhibition studies show that, despite of the high rate of hydrolysis of the acyl-enzyme, tight binding of the nucleophile and displacement of the catalytic H2O molecule...

Ngày tải lên: 08/03/2014, 08:20

... aggregates offers new possibilities in the investigation of the kinetic parameters and mechanism of violaxanthin de-epoxidation One of the advantages of such system is the defined orientation of violaxanthin ... [22] to analyse the kinetics of conversion of violaxanthin to zeaxanthin The model allowed us to follow independently the kinetics of the two de-epoxidation steps: the conversion of violaxanthin ... application of a mathematical model of de-epoxidation for the analysis of the obtained results provide important information on the influence of membrane physical properties, kinetic parameters of violaxanthin...

Ngày tải lên: 08/03/2014, 10:20

... exception of protein, in the sections that follow the efficiency of utilization of absorbed nutrients will be assumed to be 100% The utilization of absorbed nutrients is determined by the nutrient s ... human milk’s nutrient adequacy When evaluating the nutrient adequacy of human milk, it is essential to recognize the incomplete knowledge of infant nutrient requirements in terms of relevant functional ... most evident when considering the nutrient needs of infants because of the paucity of data for estimating most nutrient requirements and the limited number of functionally relevant outcome measures...

Ngày tải lên: 08/03/2014, 22:20

... Colleges and Schools to award the Master of Science and Doctor of Philosophy NOTICE on Colleges degrees of the Southern Association of AND DISCLAIMER The Institute of Paper Science and Technology (IPST) ... detrimentalmaterialson of the AKD hvdrolvsis kinetics have not been reported This paper focuses on the J d hydrolysis kinetics of AKD at different wet-end conditions, particularly at different concentrationsof salts ... Beta-ketoEsterFormationDuring the Sizingof Paperwith AlkylketeneDimers”, TappiJ., 70(4):135-139(1987) MARTON, J., “PracticalAspectsof Alkaline Sizingon Kinetics of Alkyl Ketene Dimer Reactions:Hydrolysis of Alkyl KeteneDime?,...

Ngày tải lên: 09/03/2014, 01:20

... Reduction of FlRd by avorubredoxin reductase Spectral analysis of FlRd is complex due to the partial overlap of the optical contribution of its redox cofactors Figure shows the absorption spectrum of ... presence of an excess of NADH, two events can be deconvoluted: the reduction of [Fe-Cys4] (monitored at 560 nm) and the formation of semiquinone FMN (monitored at 390 nm after subtraction of the ... modeling of FlRd-reductase (not shown) suggest: (a) the presence of the residues competent to form H-bonds with the ribose 2Â-OH and 3Â-OH groups of NADH, and (b) the absence of a nest of positively...

Ngày tải lên: 16/03/2014, 11:20

... Length of Si nanowires using Au and Pt versus time at holding temperature of 1000 °C Fig Construction of log-scaled growth rate and reciprocals of growth Temperature for the determination of activation ... (c) HRTEM image of Si nanowire using Au (d) HRTEM image of Si nanowire using Pt Both were single crystal (e) EDS line mapping of Si nanowires using Au catalyst (f) EDS line mapping of Si nanowires ... is dependent on the type of catalyst This could be explained by the effect of the catalyst on the barrier energy to the nucleation of a new step at the growth front of the nanowires (i.e., the...

Ngày tải lên: 16/03/2014, 15:05

... Havsteen BH, Garcı´ a-Moreno M & Vazquez A (1992) Kinetics of a model of autocatalysis, coupling of a reaction in which the enzyme acts on one of its substrates J Theor Biol 154, 261–270 ´ ´ Varon ... irreversible step of pepsinogen activation Biochemistry 28, 2626–2630 33 Darvey IG (1977) Transient phase kinetics of enzyme reactions where more than one species of enzyme is present at the start of the ... a-Carmona F (2000) Kinetics of a self-amplifying substrate cycle: ADP-ATP cycling assay Biochem J 350, 237–243 36 Segel IH (1975) Kinetics of unireactant enzymes In Enzyme Kinetics (Segel IH,...

Ngày tải lên: 16/03/2014, 18:20

... as cofactor (C) Log kcat ⁄ Km with NADH as cofactor (D) Log kcat ⁄ Km with biliverdin as the variable substrate and NADH as cofactor 4420 cofactor, the kcat ⁄ Km data reveal two pK values of 5.1 ... the effect of pH on the kinetic parameters and site-directed mutagenesis studies, to gain an understanding of the function of sBVR-A in cyanobacteria and also to increase our knowledge of the mechanism ... saturating (10 lm) levels of biliverdin Initial rate data for nonsaturating levels of biliverdin show that bilirubin exhibits mixed inhibition kinetics at nonsaturating levels of biliverdin and, when...

Ngày tải lên: 23/03/2014, 05:22

... Time course of excision of 8-oxoGua (d), FapyGua (s) and FapyAde ( ) by OGG1 (B) Time course of excision of 8-oxoGua (d), FapyGua (s) and FapyAde ( ) by Fpg Means and standard deviations of three ... all plots Means of two independent experiments are shown [P], concentration of the AP product of this variant in vivo and contribute to the pathogenesis of Alzheimer’s disease Of all variants ... agreement with an earlier study of the activity and substrate specificity of OGG1-S326C [11], confirming the usefulness of this variant as a reference point for the kinetics of other OGG1 mutants Differences...

Ngày tải lên: 23/03/2014, 05:22

... Requirements of Geese Documentation of Nutrient Requirements of Ducks Documentation of Nutrient Requirements of Pheasants Documentation of Nutrient Requirements of Japanese Quail Documentation of Nutrient ... Documentation of Nutrient Requirements of Broiler Breeder Males Documentation of Nutrient Requirements of Turkeys Documentation of Nutrient Requirements of Turkey Breeders Documentation of Nutrient ... Documentation of Nutrient Requirements of Leghorn-Type Chickens in Egg Production Documentation of Nutrient Requirements of Starting and Growing Market Broilers Documentation of Nutrient Requirements of...

Ngày tải lên: 23/03/2014, 11:20

... the inactivation courses of apo-TNXI and of TNXI in the presence of mm concentrations of each of the three divalent cations (Mg2+, Mn2+, or Co2+) With the exception of apo-TNXI, whose inactivation ... studies of the mechanism of xylose isomerase Biochemistry 28, 7289–7297 Bradford MM (1976) A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of ... biophysical properties of some XIs have been studied in the presence of two different metals (e.g Mg2+ and Co2+) [43–45] For three of the enzymes studied here, the Tm in the presence of mm Mg2+ plus...

Ngày tải lên: 23/03/2014, 13:20