numerical studies of bcu methods from stability boundary perspectives

... sacrifice some of its stability It has previously been suggested that the lower thermal stability of cold-adapted enzymes is simply a consequence of the lack of selective pressure for stability [29] ... et al [37] Activation energy, Ea, was extracted from the slope of the linear regression curve obtained from an Arrhenius plot of ⁄ T versus lnkcat Stability measurements DSC measurements were performed ... energy of activation Results from the thermodynamic calculations (Table 2) reveal that there is a slight difference in the free energy of activation between the two enzymes originating from both...

Ngày tải lên: 19/02/2014, 05:20

... a ˚ 3-A dissociation of subunits interacting by a1 The base of dTTP is inserted between the a1-helix of two subunits and is stacked between the rings of Phe18 and Phe34 from the adjacent subunit ... case of Ba-TK, several hydrogen bonds form between sidechain atoms and main-chain atoms of the neighboring helices: NH1 of Arg27 is hydrogen-bonded to mainchain oxygen of Ser19, and NH1 of Arg31 ... lack of substrate in Bc-TK leaves the lasso-loop flexible The open conformation of the lasso-domain in Bc-TK resembles the lasso-loop of Ca-TK [17], where part of the loop is missing because of...

Ngày tải lên: 23/03/2014, 09:21

... Acknowledgements The present thesis is the collection of the studies conducted under the guidance of my Ph.D advisor Prof Weizhu Bao from the National University of Singapore I would like to express my sincere ... temperature of the order of 100nK is required so that λdB is of the order of interatomic spacing At the same time, a gaseous state of the system has to be maintained to avoid collision of particles ... phenomena of interacting manybody system Plenty of theoretical studies on cold dilute atomic gases were carried out and a number of labs were set up to study the properties of BECs The quantity of BEC...

Ngày tải lên: 10/09/2015, 15:51

... 3.9 72 Dynamics of center of mass Left: trajectory of total center of mass Right: the time evolution of center of mass of component one (top), time evolution of center of mass of component two ... 71 Dynamics of center of mass Left: trajectory of total center of mass Right: the time evolution of center of mass of component one (top), time evolution of center of mass of component two (bottom) ... vii 26 LIST OF TABLES viii List of Figures 1.1 Velocity-distribution data of a gas of Rubidium (Rb) atoms, confirming the discovery of a new phase of matter, the Bose-Einstein...

Ngày tải lên: 02/10/2015, 17:14

... accurate numerical methods are proposed to discretize KGS and the properties 1.4 Overview of our work of these two numerical methods are studied We test the accuracy and stability of our methods ... efficient and accurate numerical methods are proposed to discretize KGS and the properties of these two numerical methods are studied We test the accuracy and stability of our methods for KGS with ... with the periodic boundary conditions and analyze the numerical properties for these two numerical methods Then we compare the accuracy and stability of different numerical methods for the KleinGordon-Schr¨odinger...

Ngày tải lên: 27/11/2015, 11:19

Ngày tải lên: 27/11/2015, 11:19

... School of Konkuk University TABLE OF CONTENTS List of Tables v List of Figures vi Abstract ix Chapter Structure and mechanism studies of sugar epimerase from ... Mechanism Studies of Sugar Epimerase from Dictyoglomus turgidum Pham, Tan Viet Department of Advanced Technology Fusion Graduate School of Konkuk University Cellobiose 2-epimerase from Dictyoglomus ... Structure and Mechanism Studies of Sugar Epimerase fromDictyoglomus turgidum A Dissertationsubmitted to the Department of Advanced Technology Fusion and the Graduate School of Konkuk University...

Ngày tải lên: 20/05/2016, 21:08

... Numerical Studies of BCU Methods from Stability Boundary Perspectives Chapter 19: Study of Transversality Conditions of the BCU Method Chapter 20: The BCU Exit Point Method Group-Based BCU Methods: Group ... 9/24/2010 2:22:51 PM Contents 18 Numerical Studies of BCU Methods from Stability Boundary Perspectives 18.1 18.2 18.3 18.4 18.5 ix 326 Introduction 326 Stability Boundary of Network-Reduction Models ... Foundations of the Controlling UEP Method Chapter 13 Chapter 23 Numerical Methods and Numerical Justification Numerical Network Preserving BCU Methods Chapter 19 Numerical Asects and Justification of BCU...

Ngày tải lên: 30/05/2014, 23:38

... result of NADH oxidation was measured One unit of enzyme activity was defined as the reduction of lmolÆmin)1 of NADH (corresponding to the carboxylation of lmolÆmin)1 of acetyl-CoA) Quantification of ... extinction coefficient of 6200 m)1Æcm)1 One unit of enzyme activity was defined as the oxidation of lmolÆmin)1 of NADH POR enzyme assays The oxidative decarboxylation activity of POR was assayed at ... atmosphere The reduction of metronidazole was calculated using an extinction coefficient of 9300 m)1Æcm)1 One unit of enzyme activity was defined as the reduction of lmolÆmin)1 of metronidazole (corresponding...

Ngày tải lên: 16/02/2014, 09:20

... suffering from a low level of environmental chemicals such as multiple chemical sensitivity (MCS) in Japan” from obscure subjects suffering from affective chemicals.33) A POLLUTED ENVIRONMENT: CAUSE OF ... residences, chemical substances emitted from old furniture might affect the health of residents The regulation of chemical emission from furniture and the education of residents are required COMMUNICATION ... (2004) Emission of pollutants from polystyrene by the dissolution in solvents Indoor Environ., 7, 1–6 (in Japanese) 87) Katakura, Y and Kishi, R (2005) Significance of laboratory studies of neurobehavioral...

Ngày tải lên: 17/02/2014, 22:20

... levels of phosphorylation expressed in relation to percentage dThd phosphorylation of the wild-type Relative phosphorylation expressed as a relation of the percentage of dThd phosphorylation of the ... phosphorylation of dGuo was reduced markedly Structure of Dm-dNK-E52D in complex with dTTP The structure of one of the mutants, E52D, was solved ˚ using X-ray crystallography at a resolution of 2.5 A ... 1-(b-d-arabinofuranosyl)-cytosine (AraC), 1-(b-d-arabinofuranosyl)-thymine (AraT) and (E)-bromvinyl-2¢-deoxyuridine (BVDU) Mutants of catalytic residues: E52 and R105 It is evident from the kinetic...

Ngày tải lên: 19/02/2014, 02:20

... in the field of ÔpH of stability Following search strings were used: Ômaximal stability , Ômaximum stability , Ôoptimal stability , Ôoptimum stability , Ôbest stability , Ôhighest stability and ... the pH of maximum of the free energy of unfolded state The points offset from the diagonal manifest the importance of the 3D structure In each case where the 3D structure causes a shift of the ... paper The experimental optimum pH (pH of optimal stability) is taken from BRENDA website [49] The calculated optimum pH (the pH of the minimum of free energy of folding) is given in the forth column...

Ngày tải lên: 19/02/2014, 12:20

... binding of cAMP to the anti-cAMP-binding sites of the protein The distance between the sulfur atom of Cys178 and the C9C10 bond of the indole ring of Trp85, derived from the crystal structure of CRP(cAMP)2 ... the mobility of helix A of the protein As Trp13 is located in the vicinity of the activation region AR2 of the protein [1], which is responsible for the activation of the second class of E coli ... maximum of Trp13 shifted from % 342.5 nm to % 340 nm The reduction in uorescence intensity and the blue shift indicate a conformational transition of CRPW85A on binding of cAMP The effect of different...

Ngày tải lên: 20/02/2014, 23:20

... disruption of the quadruplex stack A particularly valuable protocol has recently Telomeric quadruplex stability been published that describes the practical details of UV melting studies of G-quadruplexes, ... decided advantage of fluorescent methods is their high sensitivity, providing the ability to use small volumes and low concentrations of quadruplexes One possible disadvantage of FRET methods is that ... reported from different laboratories Reported Tm values range from 56 to 63.7 °C in 100 mm Na+ and from 63 to 81.8 °C in 100 mm K+ Enthalpy values range from 38 to 72.7 kcalÆmol)1 in Na+ and from...

Ngày tải lên: 06/03/2014, 09:22

... composition of social networks over time could alter the relative availability and efficiency of different types of support because of changes in the availability of certain types of ties (Connidis ... support showed moderate levels of stability with prior levels (i.e., 1988 –1989) accounting for 29% (from spouse), 10% (from children), and 10% (from friends and relatives) of the variance in later ... participant loss.) Measures of Social Support The MacArthur battery included assessments of frequency of receipt of emotional and instrumental support, as well as the frequency of negative interactions...

Ngày tải lên: 06/03/2014, 15:20

... far distant from the side chain of Arg78 The side chain of Arg78 is less well defined, as in the case of aqueous solution, because of the absence of interactions with the side chains of Asp81 (in ... elucidate the role of the positively charged residue Arg78 and the effect of the reduction of cationicity of MBP in the triggering of multiple sclerosis Flexible docking calculations of the MBP(74–85) ... amino-acid sequence of MBP from various animal species have a significant effect on the encephalitogenicity of different determinants from MBP [5] Sequence alignment of the 74–85 sequence of guinea pig...

Ngày tải lên: 07/03/2014, 16:20

... values of the average Trp13 lifetime instead of the values of lifetimes of the resolved components Binding of cAMP to anticAMP-binding sites leads to signicant changes in the uorescing properties of ... assignments of the NH protons of histidine and tryptophan residues and the effect of binding of cAMP to CRP Biochem J 107, 304309 14 Sixl F, King RW, Bracken M & Feeney J (1990) 19 F-n.m.r studies of ... be calculated from the ratio of the rate constants kon and koff as follows: Ka ẳ kon koff 2ị Association constants (Ka) of CRP with the three investigated sequences of DNA lacF, galF and ICAPF...

Ngày tải lên: 07/03/2014, 16:20

... of the cofactors The enzyme is destabilized at low ionic strength The stability of EcIPDC in aqueous solutions is higher than that of other PDCs The rate constant of inactivation of PDC from Pisum ... integral of with respect to the indole protons is consistent with the occurrence of the enol form of indolepyruvate (Fig 1) In the course of the establishment of the equilibrium % 85% of the enol ... Structure–function studies of E cloacae IDPC (Eur J Biochem 270) 2323 Fig Scheme of the postulated biosynthesis pathway of indole-3-acetate from L-tryptophan in E cloacae including the keto-enol tautomerism of...

Ngày tải lên: 08/03/2014, 02:20

... beginning of thermal unfolding Kinetic parameters of C5a peptidase: hydrolysis of peptide and pNA substrates domains with different stability Melting of C5a peptidase in the presence of mM CaCl2 ... unaffected (Fig 5C) Reduction of the intrinsic stability of the protein in the presence of EDTA and the absence of detectable stabilization effect in the presence of mM Ca2+ indicate that C5a ... probably as a result of the precipitation of the highly hydrophobic VVA product followed by its release from the parent VVASQLRANISH peptide To assess the thermal stability of C5a peptidase, we...

Ngày tải lên: 08/03/2014, 16:20

... prevention of the uptake of foreign DNA, the degradation of intestinal mucus to facilitate colonization, and the provision of nucleotides for the cells [13] Although EndA has been isolated from many ... study, the effect of NaCl concentration on the kinetic constants and thermodynamic stability of VsEndA and VcEndA was investigated In addition, the effects of reciprocal mutations of two non-conserved ... properties of the enzymes The effect of NaCl on the thermal stability of VsEndA and VcEndA was investigated by performing differential scanning calorimetry (DSC) scans in the presence of different...

Ngày tải lên: 16/03/2014, 06:20