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... acids and agar were from BD (Franklin Lakes, NJ, USA) Ammonium sulphate and amino acids were from Merck (Darmstadt, Germany) Strains, plasmids and primers The strains and plasmids used are listed ... Aliquots of the cell cultures were diluted : 10 and : 100 and samples (5 lL) from each of the three cell concentrations were spotted on solid growth media and incubated for days at the temperatures ... mutants T56M, T58V and T56M ⁄ T58W did not alter the ability of the yeast cells to respond to temperature stress suggests that the ability to phosphorylate eEF2 at T56 and ⁄ or T58 is not crucial for...

Ngày tải lên: 07/03/2014, 05:20

... resuspended in 100 lL TE buffer (10 mm Tris, mm Na2EDTA, pH 8.0) and the suspension was supplemented with an equal volume of glass beads (0.5 mm diameter), lL of a 10% (w ⁄ v) SDS solution and 100 lL ... times for 10 s and then centrifuged at 10 000 g for 10 The supernatant was extracted once with phenol ⁄ chloroform ⁄ isoamylalcohol and twice with chloroform ⁄ isoamylalcohol (24 : v ⁄ v) and centrifuged ... specific mutation and not to some unpredictable side effects Function of hypA and hypB in hydrogenase maturation The function of hypA and hypB was investigated in more detail Because HypA and HypB were...

Ngày tải lên: 08/03/2014, 08:20

... (mean = 54 and median = 55) The genotyping success rate was in the range from 98.2 to 99.5% and the retesting of the 10% randomly selected samples was 100 % concordant MMP2 -1306 C/T and -735 C/T ... survival and recurrence when we computed the Kaplan-Meier estimates and compared differences in survival based on genotypes using a log rank test (data not shown) Page of (page number not for ... three individual genotypes, and appear in bold font if ≤ 0.05 The significance was lost after adjustment for age, sex, phenotypic index, moles, freckles, and race £Genotypes 5A6A and 6A6A combined...

Ngày tải lên: 11/08/2014, 08:21

... Castillo and coworkers [1] - and prospective trials comparing different extracorporeal circuit management strategies and providing recommendations to operators in the field are welcome and urgently ... session length, downtime and dose are progressively leading to more detailed research in the field of anticoagulaPage of (page number not for citation purposes) tion and vascular access In the ... 24 hours There are no randomized trials guiding the prescription of CRRT in children A small solute clearance of l/hour × 1.73 m2 has been recommended in paediatric patients [10] However, one...

Ngày tải lên: 13/08/2014, 11:22

... (56.9) 10( 76.9) 51 (60.0) 72 (100 ) 13 (100 ) 85 (100 ) 68 (94.4) (5.6) Combined CDC AIDS Category3 13 (100 ) (0) 81 (95.3) (4.7) 189.0 ± 5.8 1745.7 ± 64.9 427.1 ± 62.1 0.227 Virological Data Genotype ... growth phenotype viruses, or in 10 HIV-uninfected healthy controls (filled triangle) The mean systemic values of each cytokine (pg/ml) and CRP (μg/ml) are compared in the figure for R/H and S/L ... titers of IL -10, IL-22 and CRP, and the disease progression markers studied However, this was not entirely surprising given the polygenic and multifactorial nature of the disease, and the difficulties...

Ngày tải lên: 12/08/2014, 23:23

... Top 10 reasons why SOPA and PIPA Acts are unjustified and should be stopped One Power of a Vague Statement Two It is Definition not Intention Three SOPA and PIPA Affect Everyone ... and PIPA Affect Everyone Worldwide Four SOPA and PIPA Affect Everyone Worldwide Five Cause for Major Internet Damage Six User Contributed Sites Are Affected Seven Social Media Endangered Eight...

Ngày tải lên: 07/03/2016, 23:48

... Answers these questions: Are you hungry? Do you want to have some food and drinks? What would you like? New words: apple orange banana water rice ... your friends would like • Write a short passage to tell what you would like for meals • List 10 more food and drinks Bài thuyết minh • I Mục tiêu: • Sau tiết học này, học sinh có thể: * Gọi tên số ... in pairs Ask your partner what would he/she like? Group works: Ask each partner in your groups and copy down in this table: Names a What food would you like? b What drinks would you like? Lan...

Ngày tải lên: 02/06/2013, 01:26

... cavity full FALSE Minh is in the hospital FALSE Minh’s cavity is serious _ TRUE Dr Lai fills the cavity in Minh’s tooth _ FALSE Minh never forget to brush his teeth TRUE Dr ... Imagine you are a speaker of a WHO meeting You are introducing the actual situation of toothache in HoChiMinh city and the dental campaign activities You have to read the text carefully and answer ... Minh’s teeth COMPLETE THE STORY worried  Minh is very _ and Dr Lai notices smiles this She at Minh and tells him not to worry She explains one of his teeth brush cavity has a ...

Ngày tải lên: 22/06/2013, 01:26

... Tuesday , April 8th , 2008 Unit 10 LIFE ON OTHER PLANETS Lesson GETTING STARTED & LISTEN AND READ Period 60 I.GETTING STARTED What can you see in the pictures ? What are they ? What we call them ... What we call them in Vietnamese ? UFOs : Unidentified Flying Objects Look at the pictures of UFOs (Unidentified Flying Objects) Ask and answer with a partner a) Have you ever heard about UFOs ... in 1964 claimed they were captured by aliens and f) In 1971, two men _ taken aboard a spacecraft and his plane disappeared after sighting a UFO g) A pilot ...

Ngày tải lên: 24/06/2013, 01:25

... 1981, a young pilot disappeared after sighting a UFO * True or False? : II-Reading 1-In 1947, an experienced pilot in the USA saw nine large round objects 2-In 1954, a woman and her children saw a ... disappear: biến 8- (to) mistake: nhầm * True or False? : II-Reading 1-In 1947, an experienced pilot in the USA saw eight large round objects 2-In 1954, a woman and her children saw a UFO above their ... object in one of his fields and also aliens collecting soil samples f- claimed they were captured by aliens and taken aboard a spacecraft g- .and his plane disappeared after sighting a UFO h-.that...

Ngày tải lên: 29/06/2013, 01:25

... Jump (v): nhảy 10 last (v): kéo dài II Listen * Check T/ F statements about the moon T … a, There is no air on the moon … b, There are rivers and lakes on the moon F … c, There are no sounds on ... the moon lasts for two weeks * Correct the false statements: a, There is no air on the moon b, There are no rivers and lakes on the moon c, There are no sounds on the moon d, It is very cold ... There are rivers and lakes on the moon … c, There are no sounds on the moon … d, It is very cold at night on the moon … e, During the day the temperature is even lower … f, There are great round...

Ngày tải lên: 07/07/2013, 01:27

... 1978 who disappeared after sighting a UFO ? 10 • Write the notes on page 84 in your notebook • Summarize the evidence of the existence of UFOs • Prepare some information about Mars and Moon 1 ... in 1964 claimed they were captured by aliens and f) In 1971, two men _ taken aboard a spacecraft and his plane disappeared after sighting a UFO g) A pilot ... for the existence of UFOs Exist (v) – Existence (n) Evidence (n) UNIT 10: LIFE ON OTHER PLANETS Getting started: Listen and Read: * Vocabulary: spacecraft (n) aircraft (n) meteor (n) = Falling...

Ngày tải lên: 01/08/2013, 05:43

... Are you and your husband the same age? Are you _ your husband? You work harder than I I don’t Dogs are friendlier than cats ... Orlando, Florida, USA, and covering a large area of land 10 Do you know the policeman coming toward us? LESSON 28: EXERCISES (IF SENTENCES) I/ Delete the incorrect verb form send ; ‘ll tell are; ... marathon, who was breathing deeply and smiling at the crowd, raised her right hand and waved ………………………………………………….…………………………………………………………… ………………………… 10 Any student who does not want to go on the trip...

Ngày tải lên: 17/08/2013, 19:10

Ngày tải lên: 05/09/2013, 23:10

... garbage Farbric Metal Glass Vegetable matter Name the garbage Paper, old or used newspaper, shoes, sandals, clothes, fruits peels, vegetable, bottle, plastic bag, comb, food can, tins, bowls, bucket, ... screw-driver , box, school bag, can, plastic bottle, glass bottle Paper, old or used newspaper, shoes, sandals, clothes, fruits peels, vegetable, bottle, plastic bag, comb, food can, tins, bowls, bucket, ... b) Where is the best place for a compose heap A a place that get no sun B a place that get sun and shade c) Should you water the compost? A Yes B No d) How long does the it take before you can...

Ngày tải lên: 09/09/2013, 04:10

... electron ngồi ngun tố là: a b 10 c d.Kếtquả khác Câu 29 N.tử M có cấu hình electron 1s22s22p4 phân bố electron obitan là: A ↑↓ ↑↓ ↑↓ ↑↓ C ↑↓ ↑↑ ↑↓ ↑ B ↑ ↑↓ ↑↓ D ↑↓ ↑↓ ↑↓ ↑ ↑ 67 10 Z X Và có cấu hình ... (e) ngun tố X : A 1s22s22p63s23p63d84s2 B 1s22s22p63s23p64s23d9 C 1s22s22p63s23p64s13d10 D 1s22s22p63s23p63d104s1 PhÇn2.Tù ln Bµi : Anion X-, Cation Y2+, Anion Z2-®Ịu cã cÊu h×nh e líp ngoµi lµ ... là: A 1s22s22p63s23p63d34s2 B 1s22s22p63s23p64s23d3 C 1s22s22p63s23p63d54s2 D 1s22s22p63s23p63d104s24p3 Câu32.A, B hai ngun tố thuộc phân nhóm thuộc chu kì liên tiếp bảng hệ thống tuần hồn Tổng...

Ngày tải lên: 29/09/2013, 18:10

... dependency and a longing for good and heroic parents? Or is it true that no matter how competent managers are, their leadership stagnates because of their limitations in visualizing purposes and generating ... harmony between what they expect and what they are able to realize from life But suppose the pains of separation are amplified by a combination of parental demands and individual needs to the degree ... managers and leaders are very different kinds of people They differ in motivation, personal history, and in how they think and act Attitudes Toward Goals Managers tend to adopt impersonal, if not...

Ngày tải lên: 24/10/2013, 07:15

... tree and namespace • The child domain trusts the parent domain • The parent domain trusts the child domain • The trusts between parent and child domains are transitive 16 Module 10: Creating and ... configuration information, and schema, are replicated Not all objects and attributes to all domain controllers in the domain are replicated For example, if the network uses a slow wide area network (WAN) ... Database and Log Locations Specify locations for the Active Directory database and log files Shared System Volume Specify the location for the shared system volume 10 Module 10: Creating and Managing...

Ngày tải lên: 26/10/2013, 23:15